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CD80 antibody | IL-A159

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Anti Bovine CD80 Antibody, clone IL-A159 thumbnail image 1 Anti Bovine CD80 Antibody, clone IL-A159 thumbnail image 2 Anti Bovine CD80 Antibody, clone IL-A159 thumbnail image 3 Anti Bovine CD80 Antibody, clone IL-A159 thumbnail image 4 Anti Bovine CD80 Antibody, clone IL-A159 thumbnail image 5 Anti Bovine CD80 Antibody, clone IL-A159 thumbnail image 6
Anti Bovine CD80 Antibody, clone IL-A159 gallery image 1
Figure A. FITC conjugated mouse anti bovine CD14 (MCA2678F) and RPE conjugated mouse IgG1 isotype control (MCA928PE). Figure B. FITC conjugated mouse anti bovine CD14 (MCA2678F) and RPE conjugated mouse anti bovine CD80 (MCA2436PE). All experiments performed on red cell lysed bovine blood gated on lymphocytes. Data acquired on the ZE5 Cell Analyzer.
Anti Bovine CD80 Antibody, clone IL-A159 gallery image 2
Figure A. RPE conjugated mouse anti bovine CD80 (MCA2436PE) and FITC conjugated mouse IgG1 isotype control (MCA928F). Figure B. RPE conjugated mouse anti bovine CD80 (MCA2436PE) and FITC conjugated mouse anti bovine CD14 (MCA2678F). All experiments performed on red cell lysed bovine blood gated on lymphocytes. Data acquired on the ZE5 Cell Analyzer.
Anti Bovine CD80 Antibody, clone IL-A159 gallery image 3
Published customer image:
Mouse anti Bovine CD80 antibody, clone IL-A159 (MCA2436GA) used for the identification of CD80 expressing lymph node cells from naïve and contagious bovine pleuropneumonia infected infetcted cattle
Image caption:
Effect of free Mmm galactan on surface expression of CD40 ( A ) and CD80 ( B ) on bovine macrophages. Cells were incubated for 48 h with galactan (EPS) at 0.1, 1 and 10 μg/mL, or with LPS and medium only (C−) as positive and negative controls respectively. Cell-surface expression of CD40/CD80 was measured by flow cytometry and results expressed as mean (±SD) percentages of positive cells for a given marker (n = 3). Asterisks indicate statistically significant differences (p < 0.05) with the negative control.

From: Totté P, Puech C, Rodrigues V, Bertin C, Manso-Silvan L, Thiaucourt F.
Free exopolysaccharide from Mycoplasma mycoides subsp. mycoides possesses anti-inflammatory properties.
Vet Res. 2015 Oct 21;46(1):122.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Anti Bovine CD80 Antibody, clone IL-A159 gallery image 4
Figure A. FITC conjugated mouse anti bovine CD62L (MCA1649F) and RPE conjugated mouse IgG1 isotype control (MCA928PE). Figure B. FITC conjugated mouse anti bovine CD62L (MCA1649F) and RPE conjugated mouse anti bovine CD80 (MCA2436PE). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.
Anti Bovine CD80 Antibody, clone IL-A159 gallery image 5
Figure A. RPE conjugated mouse anti bovine CD80 (MCA2436PE) and FITC conjugated mouse IgG1 isotype control (MCA928F). Figure B. RPE conjugated mouse anti bovine CD80 (MCA2436PE) and FITC conjugated mouse anti bovine CD62L (MCA1649F). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.
Anti Bovine CD80 Antibody, clone IL-A159 gallery image 6
Figure A. FITC conjugated mouse anti bovine CD14 (MCA2678F) and RPE conjugated mouse IgG1 isotype control (MCA928PE). Figure B. FITC conjugated mouse anti bovine CD14 (MCA2678F) and RPE conjugated mouse anti bovine CD80 (MCA2436PE). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.

Mouse anti Bovine CD80:RPE

Product Type
Monoclonal Antibody
Clone
IL-A159
Isotype
IgG1
Specificity
CD80

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Product Code Applications Pack Size List Price Your Price Qty
MCA2436PE
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 100 Tests loader
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Mouse anti Bovine CD80 antibody, clone IL-A159 recognizes the bovine CD80 cell surface antigen, expressed by dendritic cells, activated macrophages and activated B cells. CD80 plays a key role in co-stimulation of T cells during the primary immune response.

Target Species
Bovine
Species Cross-Reactivity
Target SpeciesCross Reactivity
Sheep
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the X63.Ag8.653 myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE 488nm laser 496 578
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at +4oC.
Following reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/50
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:RPE MCA928PE F 100 Tests
>
List Price Your Price
Description Mouse IgG1 Negative Control:RPE

Description Product Code Applications Pack Size List Price Your Price Quantity
Bovine Dendritic Cell Growth Kit PBP014KZZ FN 1 ml
List Price Your Price
Description Bovine Dendritic Cell Growth Kit
Bovine Dendritic Cell Growth Kit PBP015KZZ FN 5 ml
List Price Your Price
Description Bovine Dendritic Cell Growth Kit

References for CD80 antibody

  1. Glew, E.J. et al. (2003) Differential effects of bovine viral diarrhoea virus on monocytes and dendritic cells.
    J Gen Virol. 84 (Pt 7): 1771-80.
  2. Rhodes, S.G. et al. (2003) 1,25-dihydroxyvitamin D3 and development of tuberculosis in cattle.
    Clin Diagn Lab Immunol. 10 (6): 1129-35.
  3. Epardaud, M. et al. (2004) Enrichment for a CD26hi SIRP- subset in lymph dendritic cells from the upper aero-digestive tract.
    J Leukoc Biol. 76 (3): 553-61.
  4. Langelaar, M.F. et al. (2005) Mycobacterium avium ssp. paratuberculosis recombinant heat shock protein 70 interaction with different bovine antigen-presenting cells.
    Scand J Immunol. 61 (3): 242-50.
  5. Bonneau, M. et al. (2006) Migratory monocytes and granulocytes are major lymphatic carriers of Salmonella from tissue to draining lymph node.
    J Leukoc Biol. 79: 268-76.
  6. Hart, J. et al. (2011) Theileria annulata-transformed cell lines are efficient antigen-presenting cells for in vitro analysis of CD8 T cell responses to bovine herpesvirus-1.
    Vet Res. 42: 119.
  7. Ikebuchi, R. et al. (2013) Blockade of bovine PD-1 increases T cell function and inhibits bovine leukemia virus expression in B cells in vitro.
    Vet Res. 44: 59.
  8. Totté P et al. (2015) Free exopolysaccharide from Mycoplasma mycoides subsp. mycoides possesses anti-inflammatory properties.
    Vet Res. 46 (1): 122.
    9. View The Latest Product References
  9. Corripio-Miyar, Y. et al. (2017) 1,25-Dihydroxyvitamin D3 modulates the phenotype and function of Monocyte derived dendritic cells in cattle.
    BMC Vet Res. 13 (1): 390.
  10. Risalde, M.A. et al. (2020) BVDV permissiveness and lack of expression of co-stimulatory molecules on PBMCs from calves pre-infected with BVDV.
    Comp Immunol Microbiol Infect Dis. 68: 101388.
  11. Edwards, J.H. et al. (2021) Integration and functional performance of a decellularised porcine superflexor tendon graft in an ovine model of anterior cruciate ligament reconstruction.
    Biomaterials. 279: 121204.
  12. Marzo, S. et al. (2021) Characterisation of dendritic cell frequency and phenotype in bovine afferent lymph reveals kinetic changes in costimulatory molecule expression
    Vet Immunol Immunopathol. 19 Nov: 110363.
  13. Liu, J. et al. (2020) Theileria annulata. transformation altered cell surface molecules expression and endocytic function of monocyte-derived dendritic cells.
    Ticks Tick Borne Dis. 11 (3): 101365.
  14. Stabel, J.R. et al. (2022) B cell phenotypes and maturation states in cows naturally infected with Mycobacterium avium subsp. Paratuberculosis.
    PLoS One. 17 (12): e0278313.
  15. Vafaee, T. et al. (2022) Repopulation of decellularised porcine pulmonary valves in the right ventricular outflow tract of sheep: Role of macrophages.
    J Tissue Eng. 13: 20417314221102680.
  16. Wu, H. et al. (2023) Electrical stimulation of piezoelectric BaTiO3 coated Ti6Al4V scaffolds promotes anti-inflammatory polarization of macrophages and bone repair via MAPK/JNK inhibition and OXPHOS activation.
    Biomaterials. 293: 121990.

Flow Cytometry

UniProt
O46405

MCA2436PE

152220 165936 INN1612

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