CD26 Antibody | CC69

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  • Mouse anti Bovine CD26
  • Mouse anti Bovine CD26:Alexa Fluor® 647
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    CC69
  • Isotype
    IgG1
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA1652GF, IPdatasheet pdfdatasheet pdf0.25 mg
    MCA1652G
    MCA1652A647Fdatasheet pdfdatasheet pdf100 Tests/1ml
    MCA1652A647
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Bovine CD26 antibody, clone CC69 recognizes bovine Dipeptidyl peptidase 4, also known as CD26, WC10, Adenosine deaminase complexing protein or Activation molecule 3 (Lee et al. 2001). Bovine CD26 is a 765 amino acid single pass type II transmembrane glycoprotein which can be cleaved between residues 37-38 to release a soluble form of the protein. CD26 can exist as a monomer, forms a homodimer for optimal dipeptidase activity or it can form a heterodimer with seprase (UniProt: P81425).

      CD26 is expressed by a subpopulation of CD2+ T cells, dendritic cells and weakly by most B cells and is also seen on some non-haematopoietic cells, especially gut epithelium. Myeloid cells and the majority of WC1+ve T cells do not express CD26.
    • Intended Use
    • Target Species
      Bovine
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Sheepyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
      Purified IgG conjugated to Alexa Fluor®647 - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide
      0.09%Sodium Azide
      1%Bovine Serum Albumin
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
      IgG concentration 0.05 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
      aminopeptidase activity
      integral to membrane
      dipeptidyl-peptidase activity
      endocytic vesicle
      cell adhesion
      proteolysis
      serine-type endopeptidase activity
      lamellipodium membrane
      receptor activity
      protease binding
      apical plasma membrane
      positive regulation of cell proliferation
      receptor binding
      cell surface
      negative regulation of extracellular matrix disassembly
      T cell costimulation
      invadopodium membrane
      endothelial cell migration
      protein homodimerization activity
      extracellular region
      membrane raft
    • UniProt
    • Entrez Gene
    • Acknowledgements
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/501/200
      Immunoprecipitation
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional CD26 Antibody Formats

    Formats Clone Applications Sizes available
    CD26 Antibody : Alexa Fluor® 647 CC69 F 100 Tests/1ml
    CD26 Antibody : Purified CC69 F, IP 0.25 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

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      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative ControlMCA928100 TestsF
        MCA928
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative Control:Alexa Fluor® 647MCA928A647100 Tests/1mlF
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        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Flow Cytometry

              References

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