CSF1R Antibody | ROS-AV170

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CSF1R Antibody | ROS-AV170 gallery image 1

Mouse anti Chicken CSF1R (CD115) immunohistochemical staining of quail, Cortunix cortunix, bursa of Fabricius.

Cryostat sections were cut from 4 week old quail bursa of Fabricius. Clone ROS-AV170 specifically stains the bursal secretory dendritic cells (BSDC) located in the medulla and the macrophages of the interfollicular connective tissue. Image courtesy of Dr Nandor Nagy, Semmelweis University, Hungary

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CSF1R Antibody | ROS-AV170 gallery image 2

Mouse anti Chicken CSF1R (CD115) immunohistochemical staining of guinea fowl, Agelastes meleagredisbursa of Fabricius.

Clone ROS-AV170 specifically stains the bursal secretory dendritic cells (BSDC) located in the medulla. Clone ROS-AV170 also recognizes some CSF1R+ve macrophages in the interfollicular connective tissue. Image courtesy of Dr Nandor Nagy, Semmelweis University, Hungary

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CSF1R Antibody | ROS-AV170 gallery image 3

Mouse anti Chicken CSF1R (CD115) immunohistochemical staining of Grey Patridge, Perdix perdix bursa of Fabricius.

Cryostat sections we cut from 4 week old partridge bursa of Fabricius. Clone ROS-AV170 specifically recognized membrane expressed antigen on the bursal secretory dendritic cells (BSDC) in the medulla and by the macrophages (arrows) of the interfollicular connective tissue. Image courtesy of Dr Nandor Nagy, Semmelweis University, Hungary

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CSF1R Antibody | ROS-AV170 gallery image 4

Mouse anti Chicken CSF1R (CD115) immunofluorescence staining of chicken bursa of Fabricius.

Cryostat sections of 6 week old chicken bursa of Fabricius. A) Clone ROS-AV170 identifies strong membrane-bound immunoreaction on the cell surface of the bursal secretory dendritic cells (BSDC). B) Double immunofluorescence staining with Mouse anti vimentin, clone 3B4 (green), (product code OBT3104) and Mouse anti Chicken CSF1R, clone ROS-AV170 (red), (product code MCA5956GA) shows that colocalization of vimentin with CSF1R occurs only in bursal dendritic cells. Image courtesy of Dr Nandor Nagy, Semmelweis University, Hungary

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CSF1R Antibody | ROS-AV170 gallery image 5

Staining of CSF1R transfected CHO cells with Mouse anti Chicken CSF1R (MCA5957GA) followed by FITC conjugated Goat anti Mouse IgG (STAR117F) following permeabilization with Leucoperm (BUF09)

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CSF1R Antibody | ROS-AV170 gallery image 6

Staining of CSF1R transfected CHO cells with Mouse anti Chicken CSF1R (MCA5957A488) following permeabilization with Leucoperm (BUF09)

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CSF1R Antibody | ROS-AV170 gallery image 7

Staining of CSF1R transfected CHO cells with Mouse anti Chicken CSF1R (MCA5957A647) following permeabilization with Leucoperm (BUF09)

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  • Mouse anti Chicken CSF1R:Alexa Fluor® 488
  • Mouse anti Chicken CSF1R
  • Mouse anti Chicken CSF1R:Alexa Fluor® 647
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    ROS-AV170
  • Isotype
    IgG1
3 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA5956GAC, F, IFdatasheet pdfdatasheet pdf0.1 mg
    MCA5956GA
    MCA5956A488F, IFdatasheet pdfdatasheet pdf100 Tests/1ml
    MCA5956A488
    MCA5956A647F, IFdatasheet pdfdatasheet pdf100 Tests/1ml
    MCA5956A647
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Chicken CSF1R, clone ROS-AV170, recognizes the chicken homologue of human CSF1R, also known as CD115, CSF-1R, M-CSF receptor and c-fms. CSF1R is a single pass type 1 transmembrane protein belonging to the type III protein kinase family and is the cell surface receptor for both CSF1 and IL-34. Evidence from comparative sequence analysis across a range of vertebrate species indicates that CSF1 and IL-34 interact with distinct regions of CSF1R (Garceau V. et al. 2010).

      CSF1R cell surface expression appears to be one of the earliest events in macrophage lineage cells (Garceau V. et al. 2010). Clone ROS-AV170 is a monoclonal antibody that has been generated specifically to recognize chicken CSF1R . Clone ROS-AV170 has been shown to recognize the CSF1R antigen in some, but not all closely related avian species.

      Clone ROS-AV170 secretes an antibody that is an excellent marker for chicken macrophages.
    • Intended Use
    • Target Species
      Chicken
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Gooseno
      Grey partridgeyes
      Guinea Fowlyes
      Duckno
      Quailyes
      Turkeyno
      Mouseno
      Ratno
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG conjugated to Alexa Fluor® 488 - liquid
      Purified IgG - liquid
      Purified IgG conjugated to Alexa Fluor® 647 - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
      0.09% Sodium Azide (NaN3)
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
    • Immunogen
      Purified ChCSF1R-Fc fusion protein
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.05 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 0.05 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunized Balb/c mice were fused with cells of the Sp2/0-Ag14 myeloma cell line
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch
      18 months from date of despatch
      18 months from date of despatch
    • Acknowledgements
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
      Immunofluorescence
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/501/200
      Immunofluorescence
      Immunohistology - Frozen
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
      Immunofluorescence

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional CSF1R Antibody Formats

    Formats Clone Applications Sizes available
    CSF1R Antibody : Alexa Fluor® 488 ROS-AV170 F, IF 100 Tests/1ml
    CSF1R Antibody : Alexa Fluor® 647 ROS-AV170 F, IF 100 Tests/1ml
    CSF1R Antibody : Purified ROS-AV170 C, F, IF 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117A0.5 mgE, WB
      STAR117A
      Goat anti Mouse IgG/A/M:Alk. Phos.STAR87A1 mgC, E, WB
      STAR87A
      Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GA0.1 mgF, IF
      STAR117D488GA
      Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)STAR117D549GA0.1 mgF, IF
      STAR117D549GA
      Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)STAR117D649GA0.1 mgF, IF
      STAR117D649GA
      Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)STAR117D680GA0.1 mgF, WB
      STAR117D680GA
      Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GA0.1 mgF, IF, WB
      STAR117D800GA
      Rabbit F(ab')2 anti Mouse IgG:Dylight®800STAR8D800GA0.1 mgF, IF, WB
      STAR8D800GA
      Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
      STAR117F
      Goat anti Mouse IgG:FITC (Rat Adsorbed)STAR700.5 mgF
      STAR70
      Goat anti Mouse IgG (Fc):FITCSTAR120F1 mgC, F
      STAR120F
      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
      STAR9B
      Human anti Mouse IgG1:HRPHCA036P0.1 mgE
      HCA036P
      Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117P0.5 mgE, WB
      STAR117P
      Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
      STAR77
      Goat anti Mouse IgG (Fc):HRPSTAR120P1 mgE, WB
      STAR120P
      Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
      STAR13B
      Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87P1 mgE
      STAR87P
      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
      STAR12A
      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF
      STAR76

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human anti Chicken CD25:Alexa Fluor® 647HCA173A647100 Tests/1mlF
          HCA173A647
          Mouse anti Chicken CD25MCA5925GA0.1 mgE, F, WB
          MCA5925GA
          Mouse anti Chicken CD14MCA5926GA0.1 mgE, WB
          MCA5926GA
          Mouse anti Guinea Fowl Bu-1a/bMCA5958GA0.1 mgC, IP, WB
          MCA5958GA
          Mouse anti Chicken Monocytes/MacrophagesMCA57700.25 mgC, F, P *
          MCA5770
          Mouse anti Human VimentinOBT310450 µgC, E, IF, P *, WB
          OBT3104
          Mouse anti Chicken Bu-1A:RPEMCA2170PE100 TestsF
          MCA2170PE
          Mouse anti Chicken CD45:RPEMCA2413PE100 TestsF
          MCA2413PE
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human anti Chicken CD25:Alexa Fluor® 647HCA173A647100 Tests/1mlF
          HCA173A647
          Human anti Chicken CD25:FITCHCA173F0.1 mgF
          HCA173F
          Mouse anti Chicken Bu-1A:FITCMCA2170F0.1 mgF
          MCA2170F
          Mouse anti Chicken CD14MCA5926GA0.1 mgE, WB
          MCA5926GA
          Mouse anti Human VimentinOBT310450 µgC, E, IF, P *, WB
          OBT3104
          Mouse anti Chicken Bu-1A:RPEMCA2170PE100 TestsF
          MCA2170PE
          Mouse anti Chicken CD45:RPEMCA2413PE100 TestsF
          MCA2413PE
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human anti Chicken CD25:FITCHCA173F0.1 mgF
          HCA173F
          Mouse anti Chicken Bu-1A:FITCMCA2170F0.1 mgF
          MCA2170F
          Mouse anti Chicken CD14MCA5926GA0.1 mgE, WB
          MCA5926GA
          Mouse anti Guinea Fowl Bu-1a/bMCA5958GA0.1 mgC, IP, WB
          MCA5958GA
          Mouse anti Chicken Monocytes/MacrophagesMCA57700.25 mgC, F, P *
          MCA5770
          Mouse anti Chicken Bu-1A:RPEMCA2170PE100 TestsF
          MCA2170PE
          Mouse anti Chicken CD45:RPEMCA2413PE100 TestsF
          MCA2413PE

          Recommended Positive Controls

            Histology Controls

              References

              Further Reading

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