V5-Tag Antibody | SV5-Pk2

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V5-Tag Antibody | SV5-Pk2 gallery image 1

V5 tagged protein detected with Mouse anti V5-tag (MCA2892)

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  • Mouse anti V5-Tag
  • Mouse anti V5-Tag
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    SV5-Pk2
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2892GAE, IP, WBdatasheet pdfdatasheet pdf0.1 mg
    MCA2892GA
    MCA2892E, IP, WBdatasheet pdfdatasheet pdf1 mg
    MCA2892
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti V5-Tag antibody, clone SV5-Pk2 recognizes a small epitope, termed Pk, present on the P/V proteins of the paramyxovirus, SV5. Mouse anti V5-Tag antibody, clone SV5-Pk2 has been used to detect recombinant proteins, some of which include transmembrane and secreted proteins, which have been tagged with this epitope. Usually, a 14 amino acid tag has been added to the recombinant proteins, although a smaller epitope of 9 amino acids (that as a peptide inhibit the binding of the monoclonal antibody to its native protein) has also been successfully used. The 14 amino acid epitope is; gly lys pro ile pro asn pro leu leu gly leu asp ser thr. (The 9 amino acid epitope is underlined).
    • Intended Use
    • Target Species
      Viral
    • Product Form
      Purified IgG - liquid
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide
      0.09%Sodium Azide
    • Immunogen
      A recombinant peptide corresponding to the PK tag fused to a class II - restricted T helper cell epitope.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1 mg/ml
      IgG concentration 1 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0 Ag14 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
    • Acknowledgements
      This product is manufactued under an exclusive license from the University of St. Andrews, UK.
      This product is manufactued under an exclusive license from the University of St. Andrews, UK.
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Immunoprecipitation
      Western Blotting1/1001/500
    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Immunoprecipitation
      Western Blotting1/1001/500

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional V5-Tag Antibody Formats

    Formats Clone Applications Sizes available
    V5-Tag Antibody : Purified SV5-Pk2 E, IP, WB 0.1 mg | 1 mg
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

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      Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117A0.5 mgE, WB
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      STAR9B
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      STAR117P
      Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
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      Goat anti Mouse IgG (Fc):HRPSTAR120P1 mgE, WB
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      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF
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      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117A0.5 mgE, WB
      STAR117A
      Goat anti Mouse IgG/A/M:Alk. Phos.STAR87A1 mgC, E, WB
      STAR87A
      Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GA0.1 mgF, IF
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      Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)STAR117D549GA0.1 mgF, IF
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      Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)STAR117D649GA0.1 mgF, IF
      STAR117D649GA
      Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)STAR117D680GA0.1 mgF, WB
      STAR117D680GA
      Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GA0.1 mgF, IF, WB
      STAR117D800GA
      Rabbit F(ab')2 anti Mouse IgG:Dylight®800STAR8D800GA0.1 mgF, IF, WB
      STAR8D800GA
      Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
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      Goat anti Mouse IgG:FITC (Rat Adsorbed)STAR700.5 mgF
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      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
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      Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
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      Goat anti Mouse IgG (Fc):HRPSTAR120P1 mgE, WB
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      Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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