IgG2b Antibody | MARG2b-8

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Published customer image:
Mouse anti Rat IgG2b Heavy Chain antibody, clone MARG2b-8 used for the evaluation of antibody production in rats by flow cytometry.
Image caption:
Alloantibody production was suppressed after AAV-FGL2 treatment and adoptive transfer of splenocytes. Sera were collected from naive rats, or at the moment of rejection from rats treated with AAV-control or AAV-FGL2 (rejecting at < 30 days or > 120 days after transplantation) or receiving adoptive transfers (> 120 days after transplantation). Levels of donor-specific IgG1, IgG2a, and IgG2b antibodies were evaluated by cytofluorimetry and normalized to serum from naive rats (MFI / MFI syngeneic). Two way Anova, Bonferroni post test p value * <0.5; ** <0.01; ***<0.001.

From: Bézie S, Picarda E, Tesson L, Renaudin K, Durand J, Ménoret S, et al. (2015) Fibrinogen-Like Protein 2/Fibroleukin Induces Long-Term Allograft Survival in a Rat Model through Regulatory B Cells.
PLoS ONE 10(3): e0119686.

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  • Mouse anti Rat IgG2b Heavy Chain:Biotin
  • Mouse anti Rat IgG2b Heavy Chain
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    MARG2b-8
  • Isotype
    IgG1
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA195Edatasheet pdfdatasheet pdf0.25 mg
    MCA195
    MCA195BEdatasheet pdfdatasheet pdf0.5 mg
    MCA195B
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Rat IgG2b Heavy Chain antibody, clone MARG2b-8 recognizes the rat gamma 2b immunoglobulin heavy chain, and does not cross-react with other rat immunoglobulin classes or subclasses.

      The avidity of Mouse anti Rat IgG2b Heavy Chain antibody, clone MARG2b-8 for rat IgG2b = 4.7 x 109 M-1.
    • Intended Use
    • Target Species
      Rat
    • Product Form
      Purified IgG conjugated to Biotin - liquid
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography from tissue culture supernatant
      Purified IgG prepared by affinity chromatography from tissue culture supernatant
    • Preservative Stabilisers
      0.1%Sodium Azide
      50%Glycerol
      0.1% Sodium Azide (NaN3)
    • Immunogen
      Rat IgG2b myeloma protein (IR863)
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised BALB/c mice were fused with cells of the mouse Sp2/0 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
    • UniProt
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA500ng/ml
    • Application NameYesNoMin DilutionMax Dilution
      ELISA5ug/ml (as a coating antibody)

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional IgG2b Antibody Formats

    Formats Clone Applications Sizes available
    IgG2b Antibody : Purified MARG2b-8 E 0.25 mg
    IgG2b Antibody : Biotin MARG2b-8 E 0.5 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

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              References

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