IgA Antibody | MARA-1

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  • Mouse anti Rat IgA Heavy Chain
  • Mouse anti Rat IgA Heavy Chain:FITC
  • Mouse anti Rat IgA Heavy Chain:Biotin
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    MARA-1
  • Isotype
    IgG1
3 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA191C, E, IFdatasheet pdfdatasheet pdf0.25 mg
    MCA191
    MCA191FFdatasheet pdfdatasheet pdf0.5 mg
    MCA191F
    MCA191BEdatasheet pdfdatasheet pdf0.5 mg
    MCA191B
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Rat IgA Heavy Chain antibody, clone MARA-1 recognizes the alpha heavy chain of rat immunoglobulin. Mouse anti Rat IgA Heavy Chain antibody, clone MARA-1 shows no cross-reactivity with other rat immunoglobulin classes.
    • Intended Use
    • Target Species
      Rat
    • Product Form
      Purified IgG - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG conjugated to Biotin - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography from tissue culture supernatant
      Purified IgG prepared by affinity chromatography from tissue culture supernatant
      Purified IgG prepared by affinity chromatography from tissue culture supernatant
    • Preservative Stabilisers
      0.1% sodium azide
      0.1%Sodium Azide
      50%Glycerol
      0.1%Sodium Azide
      50%Glycerol
    • Immunogen
      Purified IR1060 IgA rat myeloma protein.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1 mg/ml
      IgG concentration 1 mg/ml
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA5ug/ml as coating antibody
      Immunofluorescence
      Immunohistology - Frozen
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry5ug/ml
    • Application NameYesNoMin DilutionMax Dilution
      ELISA500ng/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
      Mouse anti Rat IgA Heavy Chain antibody, clone MARA-1 may be used as a coating antibody in a sandwich ELISA in combination with Mouse anti Rat κ/λ antibody, clone MARK-1/MARL-15 (Product code MCA1296P) as detection reagent and purified rat IgA as antigen..
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional IgA Antibody Formats

    Formats Clone Applications Sizes available
    IgA Antibody : Purified MARA-1 C, E, IF 0.25 mg
    IgA Antibody : Biotin MARA-1 E 0.5 mg
    IgA Antibody : FITC MARA-1 F 0.5 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

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      Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117A0.5 mgE, WB
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      Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GA0.1 mgF, IF
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      Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)STAR117D680GA0.1 mgF, WB
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      Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GA0.1 mgF, IF, WB
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      Rabbit F(ab')2 anti Mouse IgG:Dylight®800STAR8D800GA0.1 mgF, IF, WB
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      Goat anti Mouse IgG:FITC (Rat Adsorbed)STAR700.5 mgF
      STAR70
      Goat anti Mouse IgG (Fc):FITCSTAR120F1 mgC, F
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      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
      STAR9B
      Human anti Mouse IgG1:HRPHCA036P0.1 mgE
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      Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117P0.5 mgE, WB
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      Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
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      Goat anti Mouse IgG (Fc):HRPSTAR120P1 mgE, WB
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      Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
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      Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87P1 mgE
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      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
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      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF
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      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Streptavidin:APCSTAR119100 TestsF
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      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative Control:FITCMCA1209F0.1 mgF
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        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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