CD163 Antibody | ED2

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CD163 Antibody | ED2 gallery image 1

Staining of rat peritoneal macrophages with Mouse anti Rat CD163: FITC (MCA342F)

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CD163 Antibody | ED2 gallery image 2

Staining of rat peritoneal macrophages with Mouse anti Rat CD163: RPE (MCA342PE)

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CD163 Antibody | ED2 gallery image 3

Staining of acetone fixed, cryostat sectioned rat spleen with Mouse anti Rat CD163 (MCA342GA)

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CD163 Antibody | ED2 gallery image 4

Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD163 antibody (MCA342) followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 (STAR132P) as a detection reagent. Low power

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CD163 Antibody | ED2 gallery image 5

Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD163 antibody (MCA342) followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 (STAR132P) as a detection reagent. Medium power

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CD163 Antibody | ED2 gallery image 6

Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD163 antibody (MCA342) followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 (STAR132P) as a detection reagent. Medium power

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CD163 Antibody | ED2 gallery image 7

Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD163 antibody (MCA342) followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 (STAR132P) as a detection reagent. High power

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CD163 Antibody | ED2 gallery image 8

Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD163 antibody (MCA342), red an A and Mouse anti Rat CD8 (MCA48), green in B. C is the merged image with nuclei counter-stained blue using DAPI. Low power

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CD163 Antibody | ED2 gallery image 9

Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD163 antibody (MCA342), red an A and Mouse anti Rat CD8 (MCA48), green in B. C is the merged image with nuclei counter-stained blue using DAPI. High power

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CD163 Antibody | ED2 gallery image 10

Published customer image:
Mouse anti Rat CD163, clone ED2 used for the demonstration of infiltrating macrophages in corneal graft tissue by immunohistochemistry on acetone fixed cryosections.
Image caption:
Analysis of innate immune cells into a corneal graft. Ox-62+ DC and CD163+ macrophages were stained at the time points of corneal allograft rejection and calculated within the graft. Additionally CD161+ cells were counted within rejected corneal allografts to finally prove the efficacy of the depletion protocol in the peripheral tissue. Representative histological staining is shown for Ox-62 (A), CD163 (C), and CD161 (E) in NK deficient and control animals. B: No statistical difference was observed for infiltrating Ox-62+ DC. D: CD163+ macrophages infiltrated to a statistically significantly stronger extent in 3.2.3-treated animals when compared to control treated animals (*p<0.01). F: No CD161+ cells were stained in 3.2.3-treated recipients when compared to control treated control animals (**p<0.001).

From: Schwartzkopff J, Schlereth SL, Berger M, Bredow L, Birnbaum F, Böhringer D, Reinhard T. NK cell depletion delays corneal allograft rejection in baby rats.
Mol Vis. 2010 Oct 2;16:1928-35.

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CD163 Antibody | ED2 gallery image 11

Published customer image:
Mouse anti rat CD163, clone ED2 used for the evaluation of CD163 expression in temporomandibular joint cartilage by immunohistochemistry on paraffin embedded tissue sections and western blotting on cartilage lysate.
Image caption:
Enhanced phagocytic activity and increased CD163 and TNF-α expression in degraded TMJ cartilage. A: The gross surface morphology of rat temporomandibular joint (TMJ) condyles from control (4C) and experimental (4E, 8E, 12E) groups. Pit lesions are indicated by arrows. B: Comparison of the mRNA levels of MMP-3, MMP-9, CD163, TNF-α, IL-1, ACP-1, integrin-β1 and integrin-α4 in the condylar cartilage of control (C) and experimental (E) groups. C: Transmission electron micrographs of TMJ cartilage from control group (left top panel) and the regions with grossly damaged cartilage from experimental groups (the others panels). The apoptotic (outlined with the red dashed line) and necrotic chondrocytes are shown by arrow heads. Note that within the degraded TMJ cartilage some cells were phagocytizing neighboring apoptotic and necrotic cells. D: Serial sections of condylar cartilage from the 8-week old control (upper panels) and experimental (lower panels) groups, stained with H&E (HE), or co-stained with CD163 and TUNEL. F: fibrous layer; P: proliferative layer; H: hypertrophic layer. E: Comparison of the protein levels of CD163 and TNF-α in the condylar cartilage of control (C) and experimental (E) groups by Western blotting (left panel). Graph representing the quantification of the Western blotting results, normalized to the expression of ß-actin. *P<0.05, **P<0.01. 4C: 4-week old control group; 4E: 4-week old experimental group; 8C: 8-week old control group; 8E: 8-week old experimental group; 12C: 12-week old control group; 12E: 12-week old experimental group.

From: Jiao K, Zhang J, Zhang M, Wei Y, Wu Y, Qiu ZY, et al. (2013) The Identification of CD163 Expressing Phagocytic Chondrocytes in Joint Cartilage and Its Novel Scavenger Role in Cartilage Degradation.
PLoS ONE 8(1): e53312.

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CD163 Antibody | ED2 gallery image 12

Published customer image:
Mouse anti rat CD163, clone ED2 used for the evaluation of CD163 expression in temporomandibular joint cartilage cells by immunofluorescence microscopy and flow cytometry.
Image caption:
Increase in CD163+ cells with enhanced phagocytic activity in experimentally-induced arthritic cartilage of rat TMJs. A: Flow cytometry analysis and comparison of the percentage of total CD163+ cells and CD163+ cells with phagocytic activity within isolated type II collagen expressing (Col-II+) cells from TMJ cartilage from the 8-week experimental group and their age-matched controls. B: Confocal microscope images of the CD163+ cells and assessment of their phagocytic activity in primary cells isolated from TMJ cartilage. The images reveal an increase in CD163+ cells and enhanced co-localization with the FITC-labeled cell debris in 8-week experimental group compared with the age-matched controls. C: Serial confocal images (1–4) of the primary cells isolated from TMJ cartilage of 3-week old rats co-cultured with DiO-labeled cellular debris. Sections were stained with a CD163 antibody and a Cy3-conjugated secondary antibody. Note that the CD163+ cells showed membrane staining (red) and the cell debris (green) was located inside the cell membrane. D: Dynamic observation of the phagocytic process involving living CD163+ cells sorted from TMJ cartilage engulfing cellular debris. Note that the DiO-labeled debris is undergoing phagocytosis by the CD163+ cell indicated within the white box. E: Comparison of the nitric oxide (NO) concentration and amount of intracellular reactive oxygen species (ROS) in the primary cells isolated from TMJ cartilage from 8-week experimental group and their age-matched controls. *P<0.05.

From: Jiao K, Zhang J, Zhang M, Wei Y, Wu Y, Qiu ZY, et al. (2013) The Identification of CD163 Expressing Phagocytic Chondrocytes in Joint Cartilage and Its Novel Scavenger Role in Cartilage Degradation.
PLoS ONE 8(1): e53312.

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CD163 Antibody | ED2 gallery image 13

Published customer image:
Mouse anti rat CD163, clone ED2 used for the evaluation of CD163 expression in temporomandibular joint cartilage by immunohistochemistry on paraqffin embedded tissue sections and and flow cytometry on isolated cells.
Image caption:
CD163+ chondrocytes in normal joint cartilage of 3-week old rats. A: Immunohistochemical staining of CD163 in cartilage from the TMJ and knee. The CD163+ cells located below the superior zone of the TMJ and knee cartilage, show intense membrane and cytoplasmic staining (arrows). Rat liver and muscle were selected as positive and negative controls, respectively, for the detection of CD163. Membrane staining of CD163+ cells was observed in liver (indicated by arrows), but no CD163+ cells were detected in muscle. As additional controls, TMJ and knee cartilage was also stained with an isotype control antibody. B: Flow cytometric analysis and graphical representation of the percentage of total CD163+ cells and CD163+ cells with phagocytic activity within the Col-II+ cells isolated from TMJ cartilage (n = 3; *P<0.05).

From: Jiao K, Zhang J, Zhang M, Wei Y, Wu Y, Qiu ZY, et al. (2013) The Identification of CD163 Expressing Phagocytic Chondrocytes in Joint Cartilage and Its Novel Scavenger Role in Cartilage Degradation.
PLoS ONE 8(1): e53312.

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CD163 Antibody | ED2 gallery image 14

Published customer image:
Mouse anti rat CD163, clone ED2 used for the evaluation of CD163 expression in temporomandibular joint cartilage cells by flow cytometry.
Image caption:
CD163+ chondrocytes in normal joint cartilage of 3-week old rats. A: Immunohistochemical staining of CD163 in cartilage from the TMJ and knee. The CD163+ cells located below the superior zone of the TMJ and knee cartilage, show intense membrane and cytoplasmic staining (arrows). Rat liver and muscle were selected as positive and negative controls, respectively, for the detection of CD163. Membrane staining of CD163+ cells was observed in liver (indicated by arrows), but no CD163+ cells were detected in muscle. As additional controls, TMJ and knee cartilage was also stained with an isotype control antibody. B: Flow cytometric analysis and graphical representation of the percentage of total CD163+ cells and CD163+ cells with phagocytic activity within the Col-II+ cells isolated from TMJ cartilage (n = 3; *P<0.05).

From: Jiao K, Zhang J, Zhang M, Wei Y, Wu Y, Qiu ZY, et al. (2013) The Identification of CD163 Expressing Phagocytic Chondrocytes in Joint Cartilage and Its Novel Scavenger Role in Cartilage Degradation.
PLoS ONE 8(1): e53312.

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CD163 Antibody | ED2 gallery image 15

Published customer image:
Mouse anti rat CD163, clone ED2 used for the evaluation of CD163 expression in temporomandibular joint cartilage by flow cytometry on isolated cells.
Image caption:
Exogenous TNF-α increased CD163 expression in primary chondrocytes from TMJ cartilage of 3-week old rats. A: The primary cells isolated from TMJ cartilage of 3-week old rats were positive for type II collagen (Col-II) and aggrecan, as detected by immunofluorescence and toluidine blue, respectively (400× magnification). B: A time-course of induction of CD163 mRNA expression in primary cells isolated from TMJ cartilage and treated with 10 ng/ml of TNF-α. C–D: Flow cytometric analysis and graphical representation of the percentage of CD163+ cells within the primary cells isolated from TMJ cartilage and treated with 10 ng/ml of TNF-α.

From: Jiao K, Zhang J, Zhang M, Wei Y, Wu Y, Qiu ZY, et al. (2013) The Identification of CD163 Expressing Phagocytic Chondrocytes in Joint Cartilage and Its Novel Scavenger Role in Cartilage Degradation.
PLoS ONE 8(1): e53312.

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CD163 Antibody | ED2 gallery image 16

Published customer image:
Mouse anti rat CD163, clone ED2 used for the evaluation of CD163 expression in temporomandibular joint cartilage by immunofluorescence microscopy on isolated cells.
Image caption:
TNF-a increased the phagocytic and migratory activities of CD163+ cells isolated from rat TMJ cartilage. A–B: Confocal microscope images (A) and graphical representation (B) of the numbers of CD163+ cells and their phagocytic activity within primary cells isolated from TMJ cartilage and treated with vehicle, TNF-α alone, or TNF-α and a CD163 neutralizing antibody. The co-localization of the CD163+ cell with DiO-labeled cell debris (arrows), indicates that the cell debris is undergoing phagocytosis by the CD163+ cells, as shown in the insets. Bar: 50 μm. C: Transwell assay combined with immunohistochemical staining of CD163 indicates the migratory potential of CD163+ cells in response to 10 ng/ml TNF-α, which is impaired in the presence of the TNF-α neutralizing antibody (AT, 1 μg/ml). Arrows indicate the migrating CD163+ cells. Five fields were selected at random (at 200× magnification), and the number of CD163+ cells and total cells in each image were counted. **P<0.01..

From: Jiao K, Zhang J, Zhang M, Wei Y, Wu Y, Qiu ZY, et al. (2013) The Identification of CD163 Expressing Phagocytic Chondrocytes in Joint Cartilage and Its Novel Scavenger Role in Cartilage Degradation.
PLoS ONE 8(1): e53312.

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CD163 Antibody | ED2 gallery image 17

Published customer image:
Mouse anti rat CD163, clone ED2 used for the quantitation of CD163 expressing cells in bronchiolar alvage by flow cytometry and immunofluorescence.
Image caption:
Quantification of CD163+ BAL cells by flow-cytometry. Fig (3a) shows representative flow-cytometry histograms from studied groups. Fluorescence was compared to unstained controls for 35,000 events in each histogram display, and the stained APC-CD163 positive events were compensated for unstained events by subtraction. A 5% error margin was accepted in the unstained image. GLN and IL-1/LPS showed no interaction on the percentage of CD163+ events (3b). The percentage of CD163+ events was significantly increased by GLN but not affected by IL-1/LPS. GLN and IL-1/LPS significantly interacted on the number of CD163+ macrophages (3c). CD163+ macrophage numbers were significantly increased in GLN+IL-1/LPS+ rats by both GLN and IL-1/LPS. Fig 3d shows immunofluorescence representation of CD163 stained lung regions from the GLN-IL-1/LPS+ and GLN+IL-1/LPS+ groups (white arrow points to zoomed cell in bottom right corner) (DAPI nuclear staining = blue; CD163 = red). (The significance of differences among the four groups was analyzed by two-way ANOVA. See statistical methods section for details on paired comparisons. Statistical significance was accepted as p<0.05: * compared to respective GLN- group, # compared to respective IL-1/LPS- group, ^ significant interaction between GLN and IL-1/LPS group).

From: Fernandez-Bustamante A, Agazio A, Wilson P, Elkins N, Domaleski L, He Q, et al. (2015) Brief Glutamine Pretreatment Increases Alveolar Macrophage CD163/Heme Oxygenase-1/p38-MAPK Dephosphorylation Pathway and Decreases Capillary Damage but Not Neutrophil Recruitment in IL-1/LPS-Insufflated Rats.
PLoS ONE 10(7): e0130764.

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CD163 Antibody | ED2 gallery image 18

Published customer image:
Mouse anti Rat CD163 antibody, clone ED2 used for the identification of activated macrophages in rat prostate ventral lobe by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
(A) Representative sections showing mainly non-malignant parts of ventral prostate lobe of Dunning tumor-bearing and histologically normal prostate tissue in control rats stained to visualize CD163+ macrophages (brown, 200X magnification, the tumor border (marked T) is seen in the periphery of the sections). (B) Volume density of CD163+ macrophages in the tumor-adjacent normal prostate tissue (TINT) and in controls. a; significantly different than controls, b; significantly different than corresponding tumor at day 7, and c; significantly different than corresponding tumor at day 10, P<0.05, n = 5–13.

From: Adamo HH, Strömvall K, Nilsson M, Halin Bergström S, Bergh A (2015) Adaptive (TINT) Changes in the Tumor Bearing Organ Are Related to Prostate Tumor Size and Aggressiveness.
PLoS ONE 10(11): e0141601.

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  • Mouse anti Rat CD163
  • Mouse anti Rat CD163:Biotin
  • Mouse anti Rat CD163:Alexa Fluor® 647
  • Mouse anti Rat CD163:Low Endotoxin
  • Mouse anti Rat CD163:FITC
  • Mouse anti Rat CD163
  • Mouse anti Rat CD163:RPE
(Rated 4.0 out of 5 based on 1 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    ED2
  • Isotype
    IgG1
6 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA342GAC, F, IF, IP, P *, WBdatasheet pdfdatasheet pdf0.1 mg
    MCA342GA
    MCA342BFdatasheet pdfdatasheet pdf0.1 mg
    MCA342B
    MCA342FFdatasheet pdfdatasheet pdf0.1 mg
    MCA342F
    MCA342RC, F, IF, IP, P *, WBdatasheet pdfdatasheet pdf0.25 mg
    MCA342R
    MCA342ELC, F, IF, IP, P *, WBdatasheet pdfdatasheet pdf0.5 mg
    MCA342EL
    MCA342PEFdatasheet pdfdatasheet pdf100 Tests
    MCA342PE
    MCA342A647Fdatasheet pdfdatasheet pdf100 Tests/1ml
    MCA342A647
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Rat CD163, clone ED2 recognises the rat ED2 cell surface glycoprotein (Dijkstra et al. 1985). A 175 kDa molecule also known as rat CD163, a member of the group B scavenger receptor cysteine-rich (SRCR) family and an erythroblast adhesion receptor (Fabriek et al. 2007).

      Mouse anti rat CD163, clone ED2 was shown to detect approximately 50% of peritoneal macrophages, a subset of splenic macrophages, and most tissue macrophages. However, no staining was observed in monocytes or alveolar macrophages (Dijkstra et al. 1985, Beelen et al. 1987). In freshly isolated bone marrow, expression of CD163 was limited to mature macrophages only (Barbe et al. 1990).

      Clone ED2 may be used in immunohistology using antigen retrieval, and has also been described reacting with paraffin-embedded material following PLP fixation (Periodate-lysine-paraformaldehyde), see Whiteland et al.
    • Intended Use
    • Target Species
      Rat
    • Product Form
      Purified IgG - liquid
      Purified IgG conjugated to Biotin - liquid
      Purified IgG conjugated to Alexa Fluor® 647 - liquid
      Purified IgG - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG - liquid
      Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
    • Reconstitution
      Reconstitute with 1 ml distilled water
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernanant.
    • Preservative Stabilisers
      0.09%Sodium Azide
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      None present
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      5%Sucrose
    • Immunogen
      Rat Spleen cell homogenate.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.5 mg/ml
      IgG concentration 0.1 mg/ml
      IgG concentration 0.05 mg/ml
      IgG concentration 1.0mg/ml
      IgG concentration 0.1 mg/ml
      IgG concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0-Ag 14 mouse myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      12 months from date of reconstitution.
    • Acknowledgements
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/101/100
      Immunofluorescence
      Immunohistology - Frozen1/501/100
      Immunoprecipitation
      Western Blotting
      Immunohistology - Paraffin(1)
      (1)
      This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
      Immunofluorescence
      Immunohistology - Frozen
      Immunoprecipitation
      Western Blotting
      Immunohistology - Paraffin(1)
      (1)
      This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/101/100
      Immunofluorescence
      Immunohistology - Frozen1/501/100
      Immunoprecipitation
      Western Blotting
      Immunohistology - Paraffin(1)
      (1)
      This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
      Liver
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
      Liver
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
      Liver
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional CD163 Antibody Formats

    Formats Clone Applications Sizes available
    CD163 Antibody : RPE ED2 F 100 Tests
    CD163 Antibody : Biotin ED2 F 0.1 mg
    CD163 Antibody : Alexa Fluor® 647 ED2 F 100 Tests/1ml
    CD163 Antibody : Purified ED2 C, F, IF, IP, P *, WB 0.1 mg | 0.25 mg
    CD163 Antibody : Low Endotoxin ED2 C, F, IF, IP, P *, WB 0.5 mg
    CD163 Antibody : FITC ED2 F 0.1 mg
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

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      STAR119
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117A0.5 mgE, WB
      STAR117A
      Goat anti Mouse IgG/A/M:Alk. Phos.STAR87A1 mgC, E, WB
      STAR87A
      Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GA0.1 mgF, IF
      STAR117D488GA
      Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)STAR117D549GA0.1 mgF, IF
      STAR117D549GA
      Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)STAR117D649GA0.1 mgF, IF
      STAR117D649GA
      Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)STAR117D680GA0.1 mgF, WB
      STAR117D680GA
      Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GA0.1 mgF, IF, WB
      STAR117D800GA
      Rabbit F(ab')2 anti Mouse IgG:Dylight®800STAR8D800GA0.1 mgF, IF, WB
      STAR8D800GA
      Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
      STAR117F
      Goat anti Mouse IgG:FITC (Rat Adsorbed)STAR700.5 mgF
      STAR70
      Goat anti Mouse IgG (Fc):FITCSTAR120F1 mgC, F
      STAR120F
      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
      STAR9B
      Human anti Mouse IgG1:HRPHCA036P0.1 mgE
      HCA036P
      Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117P0.5 mgE, WB
      STAR117P
      Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
      STAR77
      Goat anti Mouse IgG (Fc):HRPSTAR120P1 mgE, WB
      STAR120P
      Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
      STAR13B
      Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87P1 mgE
      STAR87P
      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
      STAR12A
      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF
      STAR76
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117A0.5 mgE, WB
      STAR117A
      Goat anti Mouse IgG/A/M:Alk. Phos.STAR87A1 mgC, E, WB
      STAR87A
      Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GA0.1 mgF, IF
      STAR117D488GA
      Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)STAR117D549GA0.1 mgF, IF
      STAR117D549GA
      Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)STAR117D649GA0.1 mgF, IF
      STAR117D649GA
      Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)STAR117D680GA0.1 mgF, WB
      STAR117D680GA
      Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GA0.1 mgF, IF, WB
      STAR117D800GA
      Rabbit F(ab')2 anti Mouse IgG:Dylight®800STAR8D800GA0.1 mgF, IF, WB
      STAR8D800GA
      Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
      STAR117F
      Goat anti Mouse IgG:FITC (Rat Adsorbed)STAR700.5 mgF
      STAR70
      Goat anti Mouse IgG (Fc):FITCSTAR120F1 mgC, F
      STAR120F
      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
      STAR9B
      Human anti Mouse IgG1:HRPHCA036P0.1 mgE
      HCA036P
      Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117P0.5 mgE, WB
      STAR117P
      Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
      STAR77
      Goat anti Mouse IgG (Fc):HRPSTAR120P1 mgE, WB
      STAR120P
      Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
      STAR13B
      Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87P1 mgE
      STAR87P
      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
      STAR12A
      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF
      STAR76

      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative ControlMCA12090.1 mgF
        MCA1209
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative Control:BiotinMCA1209B0.1 mgF
        MCA1209B
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative Control:Alexa Fluor® 647MCA1209A647100 Tests/1mlF
        MCA1209A647
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative Control:Low EndotoxinMCA1209EL0.5 mgF
        MCA1209EL
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative Control:FITCMCA1209F0.1 mgF
        MCA1209F
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative ControlMCA12090.1 mgF
        MCA1209
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative Control:RPEMCA1209PE100 TestsF
        MCA1209PE

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              Liver
              Liver
              Liver

              References

              Write your review

              ED2-AlexaFluo647
              4/5stars
              by
              Submitted: 6 Nov 2014
              Antibody used for FACS, dilution 1:10, good signal.

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