CD71 Antibody | OX-26

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CD71 Antibody | OX-26 gallery image 1

Staining of mouse spleen cells with Mouse anti Rat CD71:Low Endotoxin (MCA155EL)

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CD71 Antibody | OX-26 gallery image 2

Staining of mouse spleen with Mouse anti Rat CD71:Biotin (MCA155B)

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CD71 Antibody | OX-26 gallery image 3

Staining of rat spleen cells with Mouse anti Rat CD71:RPE (MCA155PE)

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CD71 Antibody | OX-26 gallery image 4

Staining of rat spleen lymphocytes with Mouse anti Rat CD71 (MCA155G)

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CD71 Antibody | OX-26 gallery image 5

Published customer image:
FITC conjugated Mouse anti Rat CD71 antibody, clone OX-26 used for the evaluation of CD71 expression on hair follicle stem cells by flow cytometry.
Image caption:
Flow cytometric analysis of follicle stem cells separated by Vario MACS. (a–d) Single-color flow cytometric analysis of the selected follicle stem cells. X axis represents fluorescence intensity, and Y axis represents cell number. (a) Bulge region cell suspension without antibody staining was used as control. (b) CD34bri cells were isolated by Vario MACS. (c) Control for α6 separation. (d) α6bri cells were isolated by Vario MACS. (e–h) Two-color flow cytometric analysis of follicle stem cells selected by Vario MACS. Cells were divided into four subpopulations: a PEbri FITCdim cells; b PEbri FITCbri cells; c PEdim FITCdim cells; and d PEdim FITCbri cells. (e) Control. (f) CD71 and CD34 was detected with FITC (X axis) and with PE (Y axis). The majority of the isolated cells were CD34briCD71dim cells, characterized by high level expression of CD34 and low level of CD71. No CD34dimCD71bri cells were detected in our experiments. (g) Control for α6 and CD71 combined separation; (h) The α6briCD71dim cells were the main subpopulation of the separated cells.

From: Huang E, Lian X, Chen W, Yang T, Yang L.
Characterization of rat hair follicle stem cells selected by vario magnetic activated cell sorting system.
Acta Histochem Cytochem. 2009 Oct 30;42(5):129-36.

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  • Mouse anti Rat CD71
  • Mouse anti Rat CD71
  • Mouse anti Rat CD71
  • Mouse anti Rat CD71:RPE
  • Mouse anti Rat CD71:FITC
  • Mouse anti Rat CD71:Biotin
  • Mouse anti Rat CD71:Low Endotoxin
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  • Product Type
    Monoclonal Antibody
  • Clone
    OX-26
  • Isotype
    IgG2a
5 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA155GAC, F, IF, Pdatasheet pdfdatasheet pdf0.1 mg
    MCA155GA
    MCA155FTFdatasheet pdfdatasheet pdf0.1 mg
    MCA155FT
    MCA155BFdatasheet pdfdatasheet pdf0.1 mg
    MCA155B
    MCA155RC, F, IF, P, WBdatasheet pdfdatasheet pdf0.25 mg
    MCA155R
    MCA155ELC, F, IF, P, WBdatasheet pdfdatasheet pdf0.5 mg
    MCA155EL
    MCA155GC, F, IF, P, WBdatasheet pdfdatasheet pdf1 mg
    MCA155G
    MCA155PEFdatasheet pdfdatasheet pdf100 Tests
    MCA155PE
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Rat CD71 antibody, clone OX-26 recognizes rat CD71, also known as transferrin receptor, a homodimeric type II transmembrane protein, expressed by all proliferating cells and cells with a requirement for iron, including reticulocytes and capillary endothelium in brain. Clone 0X-26 also binds to a number of non-dividing normal tissues.

      The balance between a sufficient amount of iron uptake and prevention of accumulation of excess iron within a cell, is vitally important to maintain cellular functions such as oxygen and electron transport and mitochondrial energy metabolism, whilst preventing permanent cell and tissue damage. Transferrin receptor (CD71), transferrin and ferritin have been identified as specialised proteins which control the uptake, transport and storage of free iron in tissues, thereby maintaining iron homeostasis (Crihton et al. 1992).

      An imbalance in iron homeostasis within the brain has been linked with the neurodegenerative diseases, Alzheimer’s, Parkinson’s, Huntington’s and Multiple Sclerosis (Benarroch 2009).

      Mouse anti rat CD71 clone OX-26 is reported as suitable for use in immunoelectron microscopy (Lipardi et al. 2002). OX-26 detects a band of ~95kDa in Western blotting under reducing conditions and ~195 kDa under non-reducing conditions reflecting it's homodimeric structure.
    • Intended Use
    • Target Species
      Rat
    • Product Form
      Purified IgG - liquid
      Purified IgG - liquid
      Purified IgG - liquid
      Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG conjugated to Biotin - liquid
      Purified IgG - liquid
    • Reconstitution
      Reconstitute with 1 ml distilled water
    • Preparation
      Pack Size: 0.1 mgPurified IgG prepared by affinity chromatography on Protein G
      Pack Size: 1 mg, 0.25 mgPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Pack Size: 0.1 mgPurified IgG prepared by affinity chromatography on Protein G
      Pack Size: 1 mg, 0.25 mgPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Pack Size: 0.1 mgPurified IgG prepared by affinity chromatography on Protein G
      Pack Size: 1 mg, 0.25 mgPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on protein A.
      Purified IgG prepared by affinity chromatography on Protein G
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide
      0.09%Sodium Azide
      0.09%Sodium Azide
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      5%Sucrose
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      None present.
    • Immunogen
      PHA activated rat lymphocytes.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1 mg/ml
      IgG concentration 1 mg/ml
      IgG concentration 1 mg/ml
      IgG concentration 0.1 mg/ml
      IgG concentration 0.1 mg/ml
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised Balb/c mice were fused with cells from the NS1 mouse myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      12 months from date of reconstitution.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
      peptidase activity
      melanosome
      cell surface
      proteolysis
      response to hypoxia
      response to retinoic acid
      chaperone binding
      response to iron ion
      acute-phase response
      response to manganese ion
      plasma membrane
      response to copper ion
      integral to membrane
      mitochondrion
      response to organic substance
      Hsp70 protein binding
      endocytosis
      transferrin receptor activity
      aging
      extracellular vesicular exosome
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
      1/50 Pack Size: 1 mg, 0.1 mg
      1/10 Pack Size: 0.25 mg
      1/200Pack Size: 1 mg, 0.1 mg
      1/100Pack Size: 0.25 mg
      Immunofluorescence
      Immunohistology - Frozen
      Immunohistology - Paraffin
      Western Blotting
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
      1/50 Pack Size: 1 mg, 0.1 mg
      1/10 Pack Size: 0.25 mg
      1/200Pack Size: 1 mg, 0.1 mg
      1/100Pack Size: 0.25 mg
      Immunofluorescence
      Immunohistology - Frozen
      Immunohistology - Paraffin
      Western Blotting
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
      1/50 Pack Size: 1 mg, 0.1 mg
      1/10 Pack Size: 0.25 mg
      1/200Pack Size: 1 mg, 0.1 mg
      1/100Pack Size: 0.25 mg
      Immunofluorescence
      Immunohistology - Frozen
      Immunohistology - Paraffin
      Western Blotting
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/101/100
      Immunofluorescence
      Immunohistology - Frozen
      Immunohistology - Paraffin
      Western Blotting

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional CD71 Antibody Formats

    Formats Clone Applications Sizes available
    CD71 Antibody : RPE OX-26 F 100 Tests
    CD71 Antibody : Low Endotoxin OX-26 C, F, IF, P, WB 0.5 mg
    CD71 Antibody : Purified OX-26 C, F, IF, P, WB 0.1 mg | 1 mg | 0.25 mg
    CD71 Antibody : FITC OX-26 F 0.1 mg
    CD71 Antibody : Biotin OX-26 F 0.1 mg
    • Copyright © 2016 Bio-Rad

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        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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