CD68 Antibody | ED1

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CD68 Antibody | ED1 gallery image 1

Staining of rat liver, with induced hepatocellular damage, with Mouse anti Rat CD68, clone ED1 (MCA341GA)

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Staining of rat peritoneal macrophages withAlexa Fluor®647 conjugated Mouse anti Rat CD68, clone ED1 (MCA341A647) following permeabilisation with Leucoperm (BUF09)

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Staining of rat peritoneal macrophages with Alexa Fluor® 488 conjugated Mouse anti Rat CD68, clone ED1 (MCA341A488). following permeabilisation with Leucoperm (BUF09)

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Mouse anti Rat CD68 antibody, clone ED1 used for the detection of macrophages in rat skin by immunohistochemistry.
Image caption:
Histological staining of control and HBOT wounds at post-wounding days 7 and 29. A–D) H&E staining. E–H) CD34 immunohistochemistry. I+J) CD68 immunohistochemistry.

From: uk B, Tong M, Fijneman EMG, van Neck JW (2014) Hyperbaric Oxygen Therapy to Treat Diabetes Impaired Wound Healing in Rats.
PLoS ONE 9(10): e108533.

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CD68 Antibody | ED1 gallery image 5

Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD68 antibody, clone ED1 (MCA341) followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 (STAR132P) as a detection reagent. Low power

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Mouse anti Rat CD68 antibody, clone ED1 used for the detection of macrophages in rat liver by immunohistochemistry and western blotting.
Image caption:
Effects of RPE on Kupffer cells activation in liver. (a) Hepatic CD68 expression detected with immunohistology (original magnification, ×400), (b) Hepatic CD68 expression detected with western-blot. Values represent the mean ± SD of three independent experiments. *, versus control; #, versus ethanol.

From: Jing-Hua Peng, Tuan Cui, Zhao-Lin Sun, et al., “Effects of Puerariae Radix Extract on Endotoxin Receptors and TNF- Expression Induced by Gut-Derived Endotoxin in Chronic Alcoholic Liver Injury,”
Evidence-Based Complementary and Alternative Medicine, vol. 2012, Article ID 234987, 12 pages, 2012.

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Mouse anti Rat CD68 antibody, clone ED1 used for the detection of macrophages in rat brain by western blotting.
Image caption:
Western blotting of ED1 (Marker of activated microglia) and TRAIL (tumor necrosis factor-related apoptosis-inducing ligand). Brains were treated with PBS or rAAV2/IL12 and then implanted with tumor on the last day of Week 2 post viral vector injection. The brains were used for Western blotting analysis of the expression of ED1, 34 kDa, and TRAIL, 104 kDa, from microglial cells on the last day of week 1, 2, and 3 following tumor implantation. The brain slices for Western blotting analysis are shown at the bottom. All brains were harvested on the last day of week 1, week 2, and week 3 post tumor implantation. The scale bars indicate 2 mm in brain sections.

From: The treatment of glioblastoma multiforme through activation of microglia and TRAIL induced by rAAV2-mediated IL-12 in a syngeneic rat model. Chiu TL, Wang MJ, Su CC.
J Biomed Sci. 2012 Apr 22;19:45. doi: 10.1186/1423-0127-19-45.

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CD68 Antibody | ED1 gallery image 8

Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD68 antibody, clone ED1 (MCA341) followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 (STAR132P) as a detection reagent. Medium power

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CD68 Antibody | ED1 gallery image 9

Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD68 antibody, clone ED1 (MCA341) followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 (STAR132P) as a detection reagent. High power

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Mouse anti Rat CD68 antibody, clone ED1 used for the detection of macrophages in rat brain by immunohistochemistry.
Image caption:
Immunohistochemistry of ED1 stain in brain sections treated with rAAV2/IL12 (A, B, C, D, n = 4), AAV2/GFP (E, F, n = 2), or PBS (G, H, n = 2), accompanied with tumor implantation; and that in the brain section treated with nothing (n = 1). Immunohistochemistry of brain sections was performed for ED1 on the last day of week 3 after tumor implantation. The brain sections were stained with hematoxylin for nuclei and ED1 for activated microglial cells. The 1st column shows the brain sections pictured before immunostaining; the 2nd column shows the brain sections pictured after staining; the 3rd to 6th columns show pictures taken at four quadrants (black squares in 2nd column) of the tumor adjacent to the normal tissue in the right hemisphere. ED1-positive cells show dark brown. The scale bars indicate 2 mm in 1st and 2nd columns and 100 µm in 3rd-6th columns.

From: Chiu TL, Wang MJ, Su CC. The treatment of glioblastoma multiforme through activation of microglia and TRAIL induced by rAAV2-mediated IL-12 in a syngeneic rat model.
J Biomed Sci. 2012 Apr 22;19:45.

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Mouse anti Rat CD68 antibody, clone ED1 used for the detection of macrophages in rat brain by immunohistochemistry.
Image caption:
Immunohistochemical stains of ED1 and TRAIL in brain sections treated with AAV2/IL12 (A, B) or PBS (C, D), accompanied with tumor implantation; and that in the brain treated with nothing (E). Brain sections used in Figure 4 were also stained with hematoxylin for nuclei, ED1, and TRAIL, and pictured as in Figure 4. The 1st and 2nd columns show the low and high power fields of ED1 stain, respectively; the 3rd and 4th columns show the low and high power fields of TRAIL stain. Cells stained with ED1 or TRAIL show dark brown. The scale bars indicate 100 µm in low power field and 50 µm in high power field.

From: Chiu TL, Wang MJ, Su CC. The treatment of glioblastoma multiforme through activation of microglia and TRAIL induced by rAAV2-mediated IL-12 in a syngeneic rat model.
J Biomed Sci. 2012 Apr 22;19:45.

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CD68 Antibody | ED1 gallery image 12

Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD68 antibody, clone ED1 (MCA341), red in A and Mouse anti Rat CD4 (MCA55), green in B. C is the merged image with nuclei counter-stained blue using DAPI. Low power

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Mouse anti Rat CD68 antibody, clone ED1 used for the detection of macrophages in rat brain by flow cytometry.
Image caption:
Fluorescence-activated cell sorting analysis of cells isolated from adjuvant-induced arthritis rat synovial tissue. Twenty-four hours after systemic administration of N-(2-hydroxypropyl)methacrylamide copolymer labeled with Alexa Fluor® 488 (P-Alexa), cells were isolated from adjuvant-induced arthritis rat synovial tissue for fluorescence-activated cell sorting (FACS) analysis. IgG1 was used as control for CD68 and prolyl-4-hydroxylase. IgG2a was used as control for CD11c. The result for IgG2a is similar to that for IgG1 with the upper right quadrant detected at 2.45% (data not shown).

From: Quan et al. Arthritis Research & Therapy 2010 12:R170.

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CD68 Antibody | ED1 gallery image 14

Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD68 antibody, clone ED1 (MCA341), red in A and Mouse anti Rat CD4 (MCA55), green in B. C is the merged image with nuclei counter-stained blue using DAPI. Medium power

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CD68 Antibody | ED1 gallery image 15

Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD68 antibody, clone ED1 (MCA341), red in A and Mouse anti Rat CD4 (MCA55), green in B. C is the merged image with nuclei counter-stained blue using DAPI. High power

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Mouse anti Rat CD68 antibody, clone ED1 used for the detection of macrophages in rat joints by immunofluorescence.
Image caption:
Immunohistochemical staining of sections from inflamed joints with anti-CD68 or anti-prolyl-4-hydroxylase antibodies. Representative confocal microscopic images of (a) anti-CD68 and (b) anti-prolyl-4-hydroxylase immunohistochemical staining of decalcified, Alexa Fluor® 488-labeled N-(2-hydroxypropyl)methacrylamide copolymer (P-Alexa)-treated adjuvant-induced arthritis (AA) rat ankle joint sections are summarized in this figure. Each panel is composed of four sub-images: antibody red staining, P-Alexa green signal, differential interference contrast (DIC) image and the co-localization of the three. The co-localization of red and green color in both panels yields a yellow color, which confirms the uptake of the HPMA copolymer conjugate by CD68-positive (macrophage-like) or prolyl-4-hydroxylase-positive (fibroblast-like) synoviocytes in AA rat ankle joints.

From: Quan et al. Arthritis Research & Therapy 2010 12:R170.

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Mouse anti Rat CD68 antibody, clone ED1 used for the detection of macrophages in rat brain by immunohistochemistry.
Image caption:
Immunohistochemical analysis of microglial activation and neuronal loss on day 3 post-treatment with UCN. Representative photomicrographs show: (A) low power images of peri-hematomal regions as indicated by squares; (B) high power images stained with OX-42; (C) ED-1; and (D) NeuN. (E) Average cell numbers (OX 42+, ED-1+, and NeuN+) in each plane (total 6 mm2) was calculated from 54 squares in 9 planes. Values are shown as mean ± SD, calculated and analyzed by Student's t test. *p < 0.05 vs. ICH + saline group.

From: Liew HK, Pang CY, Hsu CW, Wang MJ, Li TY, Peng HF, Kuo JS, Wang JY. Systemic administration of urocortin after intracerebral hemorrhage reduces neurological deficits and neuroinflammation in rats.
J Neuroinflammation. 2012 Jan 19;9:13.

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Mouse anti Rat CD68 antibody, clone ED1 used for the detection of macrophages in rat brain by immunofluorescence.
Image caption:
Immunohistochemical stains of ED1 and TRAIL in brain sections treated with AAV2/IL1. Immunohistochemistry for CD68 in the optic nerve. There is a cellular accumulation indicated by DAPI at the crushed sites of the optic nerve. Some of the cells are positively stained with CD68 suggesting that there is a recruitment of microglia/macrophages to the crushed sites. Bar = 100 µm.

From: Suzuki H, Oku H, Horie T, Morishita S, Tonari M, et al. (2014) Changes in Expression of Aquaporin-4 and Aquaporin-9 in Optic Nerve after Crushing in Rats.
PLoS ONE 9(12): e114694.

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Mouse anti Rat CD68 antibody, clone ED1 used for the detection of macrophages in rat optic nerve by immunofluorescence.
Image caption:
Immunohistochemical stains of ED1 and TRAIL in brain sections treated with AAV2/IL1. Immunohistochemistry for CD68 in the optic nerve. There is a cellular accumulation indicated by DAPI at the crushed sites of the optic nerve. Some of the cells are positively stained with CD68 suggesting that there is a recruitment of microglia/macrophages to the crushed sites. Bar = 100 µm.

From: Suzuki H, Oku H, Horie T, Morishita S, Tonari M, et al. (2014) Changes in Expression of Aquaporin-4 and Aquaporin-9 in Optic Nerve after Crushing in Rats.
PLoS ONE 9(12): e114694.

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Mouse anti Rat CD68 antibody, clone ED1 used for the detection of macrophages in rat optic nerve by immunofluorescence.
Image caption:
Immunohistochemical stains of ED1 and TRAIL in brain sections treated with AAV2/IL1. Immunohistochemistry for CD68 in the optic nerve. There is a cellular accumulation indicated by DAPI at the crushed sites of the optic nerve. Some of the cells are positively stained with CD68 suggesting that there is a recruitment of microglia/macrophages to the crushed sites. Bar = 100 µm.

From: Suzuki H, Oku H, Horie T, Morishita S, Tonari M, et al. (2014) Changes in Expression of Aquaporin-4 and Aquaporin-9 in Optic Nerve after Crushing in Rats.
PLoS ONE 9(12): e114694.

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Mouse anti Rat CD68 antibody, clone ED1 used for the detection of macrophages in rat optic nerve by immunofluorescence.
Image caption:
Immunohistochemical stains of ED1 and TRAIL in brain sections treated with AAV2/IL1. Immunohistochemistry for CD68 in the optic nerve. There is a cellular accumulation indicated by DAPI at the crushed sites of the optic nerve. Some of the cells are positively stained with CD68 suggesting that there is a recruitment of microglia/macrophages to the crushed sites. Bar = 100 µm.

From: Suzuki H, Oku H, Horie T, Morishita S, Tonari M, et al. (2014) Changes in Expression of Aquaporin-4 and Aquaporin-9 in Optic Nerve after Crushing in Rats.
PLoS ONE 9(12): e114694.

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Mouse anti Rat CD68 antibody, clone ED1 used for the detection of CD68 expressing cells in rat brain by immunofluorescence on free-floating sections.
Image caption:
Implantation mode and embedding dependent glial reactions. A) Representative images of rigid, flex and flex embedded mode implantations after 6 weeks, showing mode dependent alterations in astrocytosis, as shown in red (GFAP), microglia responses, as shown in green (ED1) and merged (DAPI in blue (total cell nuclei)). Scale bar: 50 µm. Cephalocaudal direction corresponds to top-to-bottom in image. B and C) Quantification of the GFAP and ED1 densities, respectively, that surrounds the different implantation sites in the inner ROI (0–50 µm). The box corresponds to the 25th and 75th percentiles, the median value is indicated by the horizontal line within each box, and the whiskers show the minimum and maximum values. The horizontal lines indicate statistical differences.

From: Köhler P, Wolff A, Ejserholm F, Wallman L, Schouenborg J, et al. (2015) Influence of Probe Flexibility and Gelatin Embedding on Neuronal Density and Glial Responses to Brain Implants.
PLoS ONE 10(3): e0119340.

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Mouse anti Rat CD68 antibody, clone ED1 used for the detection of CD68 expressing macrophages in the spleen of liposome treated rats by immunofluorescence on formalin fixed cryosections.
Image caption:
Confirmation of Macrophage Cell Depletion Examined in the Spleens of Liposome-Treated Animals. A-B) 1 week after liposome administration no macrophage cells were present in the spleens of treated animals, as confirmed by the absence of CD68 (macrophage marker) immunoreactivity (***P < 0.0001; I). C-D) By 2 week after liposome treatment completion, macrophage cells (CD68+) had already began to repopulate in the spleen of treated animals (*P < 0.05; I). E-F) At the end of the experimental period (day 35) macrophage numbers in the spleen of treated animals had returned to normal levels, as compared to untreated animals (G-H). I) Quantification of immunoreactive CD68+ cells as shown in panels A-H, demonstrated a significant cellular reduction during liposome administration and during repopulation 2 weeks after treatment completion. No differences in macrophage cells were found at the end of the study. Columns represent an averaged mean (5 sections per animal, n = 5) and error bars indicate error of mean (+/- S.E.). Scale bars A, C, E, G 500 µm, enlarged views B, D, F, H 200 µm.

From: Salegio EA, Pollard AN, Smith M, Zhou XF. Macrophage presence is essential for the regeneration of ascending afferent fibres following a conditioning sciatic nerve lesion in adult rats.
BMC Neurosci. 2011 Jan 20;12:11.

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Mouse anti Rat CD68 antibody, clone ED1 used for the detection of CD68 expressing macrophages in rat kidney by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Renal ED-1 immunostaining of the rats treated for 28 days: sham (A), losartan (B), leptin (C) and leptin plus losartan (D). ED-1-positive cell quantification (in cells/area) (E). Magnification of the leptin-treated rats ED-1 positive cell area. G = glomeruli; arrowheads = ED-1-positive cells; bar = 100 µm. *p<0.05 versus the sham; #p<0.05 versus the leptin-treated rats and $p<0.05 versus the losartan-treated. For the rats treated for 28 days: sham (n = 6); losartan (n = 6), leptin (n = 8); leptin plus losartan (n = 6).

From: Thieme K, Oliveira-Souza M (2015) Renal Hemodynamic and Morphological Changes after 7 and 28 Days of Leptin Treatment: The Participation of Angiotensin II via the AT1 Receptor.
PLoS ONE 10(3): e0122265.

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Mouse anti Rat CD68 antibody, clone ED1 used for the detection of macrophages in rat kidney by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
ED-1 (macrophage) staining from STNx rats. As illustrated by representative photomicrographs, in sham rats (A), only occasional macrophages were observed in the interstitium, while STNx rats (B) were associated with numerous macrophages. When compared to PPG (C) and Irb (D) mono-therapy, treatment of STNx animals with PPG+Irb (E) was associated with a further reduction in the number of macrophages. Magnification x200. Quantitative data (F) are expressed as mean ± SEM. *, P <0.05 vs sham; #, P <0.05 vs vehicle-treated;f, P <0.05 vs Irb-treated STNx rats. Animal numbers: Sham = 20, STNx = 19, STNx+PPG = 17, STNx+Irb = 19 and STNx+PPG+Irb = 16.

From: Ayoub MA, Zhang Y, Kelly RS, See HB, Johnstone EKM, et al. (2015) Functional Interaction between Angiotensin II Receptor Type 1 and Chemokine (C-C Motif) Receptor 2 with Implications for Chronic Kidney Disease.
PLoS ONE 10(3): e0119803.

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Mouse anti Rat CD68 antibody, clone ED-1 used for the identification of CD68 expressing cells in rat kidney by immunohistochemistry
Image caption:
ED-1 stain of kidney tissue. The groups are (A) control, (B) EG-exposed, (C) EG+STS-exposed, (D) EG+SC-exposed, (E) EG+SS-exposed animal groups. The ED-1 stain was markedly increased in EG-exposed animals but decreased in all treatment groups. The significance levels are with reference to the EG group. The data are means ± SD from 7–8 animals per group. ***p < 0.001.

From: Bijarnia RK, Bachtler M, Chandak PG, van Goor H, Pasch A (2015) Sodium Thiosulfate Ameliorates Oxidative Stress and Preserves Renal Function in Hyperoxaluric Rats. PLoS ONE 10(4): e0124881.

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Mouse anti Rat CD68 antibody, clone ED1 used for the identification of macrophages in venous thrombi by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Effects of Temporary Dietary Iron Restriction on Leukocyte Recruitment, Neovascularization, and Plasmin System of Stasis-Induced Venous Thrombi in Rats. (A) Representative images of CD68 staining of the venous thrombi. Scale bars: 100 µm. (B) Quantification of CD68 positive cells in the venous thrombi of the DVT and DVT+IR groups (n = 6 in each group). (C) Representative images of vWF staining of the venous thrombi. Scale bars: 50 μm. Arrows show vascular channels. (D) Quantification of vascular channels in the venous thrombi of the DVT and DVT+IR groups (n = 6 in each group). The number of CD68 positive cells or vascular channels in 10 randomly selected high power fields (HPF) was counted in a blinded manner. (E) CD31 protein expression (top; representative gel blots depicting expression of CD31 and GAPDH, bottom; relative levels of expression) in the venous thrombi of the DVT and DVT+IR groups (n = 5 in each group). (F) Intrathrombotic gene expression of uPA, tPA, PAI-1, and fibrinogen a chain in the DVT and DVT+IR groups (n = 4 in each group). *p < 0.05 versus the DVT group.

From: Oboshi M, Naito Y, Sawada H, Hirotani S, Iwasaku T, Okuhara Y, et al. (2015) Temporary Dietary Iron Restriction Affects the Process of Thrombus Resolution in a Rat Model of Deep Vein Thrombosis.
PLoS ONE 10(5): e0126611.

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Dot Blot showing FSC/SSC gated rat peritonial macrophages dual stained with CD68 (MCA341A488) at neat and CD11b (MCA275A647) at neat. Isotype control pair in red. Permeabilisation was with Leucoperm (BUF09)

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Mouse anti Rat CD68 antibody, clone ED-1 used for the detection of infiltrating macrophages in lung tissue by immunofluorescence.
Image caption:
IH causes the accumulation of macrophages and upregulates β3AR expression in the lungs. (A) Representative bright-field images of lung sections from the N and IH rats and images of immunofluorescent staining of such sections with anti-ED-1 antibody, anti-β3AR antibody, or both (merged images). Calibration bar = 200 μm for 40 x, 50 μm for 200 x. (B, C) The numbers of ED-1- and β3AR-positive cells per field (200 x) were counted using Image Pro Plus ver. 4.1 (n = 6 each, mean ± S.D.) (D) Ratio of the percentage of β3AR-positive cells to the percentage of ED-1-positive cells (n = 6 each, mean ± S.D.) (E) Representative images of double immunocytochemical staining using anti-ED-1 and β3AR antibody in BALF-derived macrophages. Calibration bar = 50 μm. (F) Western blot analysis of β3AR in lung homogenate solutions from the N and IH rats (n = 6 each, mean ± S.D.) (G) The expression level of β3AR mRNA in lung tissue samples from the N and IH rats (n = 6 each, mean ± S.E.M.) (H) Western blot analysis of β3AR in BALF-derived pulmonary macrophages obtained after 6 weeks of IH or normoxic exposure (n = 5 each, mean ± S.D.) *Significant difference between the N and IH rats (*P<0.05, **P<0.01).

From: Nagai H, Kuwahira I, Schwenke DO, Tsuchimochi H, Nara A, Ogura S, et al. (2015) Pulmonary Macrophages Attenuate Hypoxic Pulmonary Vasoconstriction via β3AR/iNOS Pathway in Rats Exposed to Chronic Intermittent Hypoxia.
PLoS ONE 10(7): e0131923.

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CD68 Antibody | ED1 gallery image 30

Dot Blot showing FSC/SSC gated rat peritonial macrophages dual stained with CD68 (MCA341A488) at neat and CD163 (MCA342A647) at a 1/5 dilution. Two distinct populations of CD68 positive macrophages were observed, CD163-low M1 macrophages and CD163-high M2 macrophages. Isotype control pair in red.Permeabilisation was with Leucoperm (BUF09)

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Mouse anti Rat CD68 antibody, clone ED-1 used for the detection of infiltrating macrophages in lung tissue by immunofluorescence.
Image caption:
Pulmonary macrophages in IH rats expressed pro-inflammatory markers including iNOS. The pulmonary macrophages were obtained from BALF after 6 weeks of IH or normoxic exposure. (A) Immunocytochemical staining of pro-inflammatory markers iNOS, CD11c, and IL-6 was performed. (B) Western blot analysis of iNOS, IL-6, and TNFα in BALF-derived macrophages (n = 5 each, mean ± S.D.). *Significant difference between the N and IH rats (*P<0.05).

From: Nagai H, Kuwahira I, Schwenke DO, Tsuchimochi H, Nara A, Ogura S, et al. (2015) Pulmonary Macrophages Attenuate Hypoxic Pulmonary Vasoconstriction via β3AR/iNOS Pathway in Rats Exposed to Chronic Intermittent Hypoxia.
PLoS ONE 10(7): e0131923.

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Mouse anti Rat CD68 antibody, clone ED-1 used to identify macrophages in formalin fixed, paraffin embedded rat kidney tissue sections by immunohistochemistry.
Image caption:
Decreased interstitial inflammation, increased periglomerular and glomerular macrophages after cessation of Ang II. Ang II increased the influx of interstitial and periglomerular macrophages. After Ang II withdrawal, the number of interstitial ED1 positive cells decreased, but was still elevated versus control rats (A). Periglomerular macrophages slightly increased after Ang II cessation, however this was not signifcant (B). For glomerular macrophages the opposite trend was found, with a decrease of macrophages per glomerulus after Ang II. After stopping infusion, Ang II-infused rats showed an increase in glomerular macrophages (C). Representative photomicrographs of ED-1 positive cells in interstitium (D) and in and around glomeruli (E). (# p<0.05, ## p<0.01, ### p<0.001 vs. control at time of biopsy; **p<0.01, ***p<0.001 vs. Ang II at time of biopsy; $ p<0.05 vs. control at time of sacrifice).

From: Frenay A-RS, Yazdani S, Boersema M, van der Graaf AM, Waanders F, van den Born J, et al. (2015) Incomplete Restoration of Angiotensin II - Induced Renal Extracellular Matrix Deposition and Inflammation Despite Complete Functional Recovery in Rats.
PLoS ONE 10(6): e0129732.

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Mouse anti Rat CD68 antibody, clone ED-1 used for the detection of infiltrating macrophages in lung tissue by immunofluorescence.
Image caption:
Acute intratracheal administration of clodronate restores HPV in IH rats. (A) Representative bright-field images and images of immunofluorescent staining using anti-ED-1 antibody of lung sections with or without clodronate. Clodronate (500 μg of clodronate in 100 μL of saline) was injected intratracheally just after the end of the 6-week IH/normoxia exposure period. Calibration bar = 200 µm. (B) Representative microangiographic images of the small pulmonary arteries in the N and IH rats obtained 3 days after the i.t. administration of clodronate. The black arrows point to branches that underwent vasoconstriction. (C) Relationship between vessel size and the extent of the pulmonary vasoconstriction induced in response to acute hypoxia. Data are presented as mean ± S.E.M. values. *Significant change in vessel diameter compared with the baseline conditions (**P<0.01).

From: Nagai H, Kuwahira I, Schwenke DO, Tsuchimochi H, Nara A, Ogura S, et al. (2015) Pulmonary Macrophages Attenuate Hypoxic Pulmonary Vasoconstriction via β3AR/iNOS Pathway in Rats Exposed to Chronic Intermittent Hypoxia.
PLoS ONE 10(7): e0131923.

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Mouse anti Rat CD68 antibody, clone ED-1 used for the detection of infiltrating macrophages in lung tissue by immunofluorescence.
Image caption:
An increase in the number of macrophages is observed in alveolar spaces. Representative images of immunohistochemical staining with anti-β3AR antibody in paraffin embedded lung sections. (A) Almost all intra-alveolar cells in IH rats were strongly stained by anti-β3AR antibody. In contrast, these cells in N rats were not. These results suggest that the brown cells are macrophages. Nuclei were counterstained with Haematoxylin. Calibration bar = 10 μm. (B) These images show the distribution of macrophages in alveolar spaces. Calibration bar = 50 μm. (C) The number of macrophages in alveolar spaces were significantly increased in IH rats compared to these in N rats (n = 6 each, mean ± S.D.). *Significant difference between N and IH rats (**P<0.01).

From: Nagai H, Kuwahira I, Schwenke DO, Tsuchimochi H, Nara A, Ogura S, et al. (2015) Pulmonary Macrophages Attenuate Hypoxic Pulmonary Vasoconstriction via β3AR/iNOS Pathway in Rats Exposed to Chronic Intermittent Hypoxia.
PLoS ONE 10(7): e0131923.

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Mouse anti Rat CD68 antibody, clone ED-1 used for the detection of infiltrating macrophages in lung tissue by immunofluorescence.
Image caption:
eNOS and nNOS are not upregulated in BALF-derived macrophages in IH rats. (A) Representative images of double immunocytochemical staining with anti-ED-1, and eNOS or nNOS antibody in BALF-derived macrophages. Calibration bar = 50 μm. (B) Western blot analysis of eNOS in BALF-derived macrophages (n = 5 each, mean ± S.D.) nNOS was undetectable in both N and IH rats.

From: Nagai H, Kuwahira I, Schwenke DO, Tsuchimochi H, Nara A, Ogura S, et al. (2015) Pulmonary Macrophages Attenuate Hypoxic Pulmonary Vasoconstriction via β3AR/iNOS Pathway in Rats Exposed to Chronic Intermittent Hypoxia.
PLoS ONE 10(7): e0131923.

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Mouse anti Rat CD68 antibody, clone ED1 used for the identification of macrophages in rat prostate ventral lobe by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
(A) Representative sections from the ventral prostate lobe of Dunning tumor-bearing and control rats stained to visualize CD68+ macrophages (brown, 200X magnifications, T; tumor). (B) Volume density of CD68+ macrophages in the tumor-adjacent prostate tissue (TINT) and in controls. a; significantly different than controls, b; large G tumors significantly different than small G tumors, and c; significantly different than corresponding tumor at day 7, P<0.05, n = 5–13. (C) Scatterplot of the volume density of CD68+ macrophages in the tumor-bearing organ plotted against tumor weight (correlation coefficients are given in the result text).

From: Adamo HH, Strömvall K, Nilsson M, Halin Bergström S, Bergh A (2015) Adaptive (TINT) Changes in the Tumor Bearing Organ Are Related to Prostate Tumor Size and Aggressiveness.
PLoS ONE 10(11): e0141601.

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Mouse anti Rad CD68 antibody, clone ED1 (MCA341GA) used for the identification of macrophages in mouse retina by immunohistochemistry on formalin fixed, paraffin embedded tissue sections in a model of Neuromyelitisoptica/ spectrum disorder (NMO/SD)
Image caption:
Histological characterization of inflammatory lesions in the retina. Consecutive retinal sections were reacted with antibodies against CD3, ED1, rat IgG, AQP4, GFAP, glutamine synthetase (GS), C9, and human IgG. Positive reaction products are shown in brown or red (C9 only). All sections were counterstained with hematoxylin to reveal nuclei in blue. Bars?=?100 μm. The tissue derived from animals injected with AQP4268–285-specific T cells and NMO-IgG (ENMO), with AQP4268–285-specific T cells and human control IgG (T?+?co IgG) and healthy controls, and show inflammation at the inner blood retinal barrier (IBRB), at the outer blood retinal barrier (OBRB), simultaneously at both retinal barriers (I/OBRB), or an absence of inflammation at these sites in the control animals. The insert shown for the GS stained retina of the rat injected with T cells and co IgG refers to the area labeled by a white star in the adjacent overview. It demonstrates that there is no loss of GS reactivity at this site, but the lumen of a blood vessel. Bar?=?100 μm.

From: Zeka B, Hastermann M, Kaufmann N, Schanda K, Pende M, Misu T, Rommer P, Fujihara K, Nakashima I, Dahle C, Leutmezer F, Reindl M, Lassmann H, Bradl M.
Aquaporin 4-specific T cells and NMO-IgG cause primary retinal damage in experimental NMO/SD.
Acta Neuropathol Commun. 2016 Aug 8;4(1):82.

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CD68 Antibody | ED1 gallery image 38

Published customer image:
Mouse anti RatCD68 antibody, clone ED1 (MCA341GA) used for the identification of microglia in rat brain following traumatic brain injury using immunohistochemistry. Lymphocytes (a + b) are identified using Mouse anti Rat CD43 antibody, clone W3/13 (MCA54G) in the same experiment.
Image caption:
AhR double labeling in brain sections from day 1 after TBI. a Most AhR+ non-neuronal cells (brown) co-expressed W3/13 (blue). The boxed areas indicate the regions that were further observed under high-power magnification shown in (b). c and d: However, most AhR+ non-neuronal cells (blue) did not co-localize with ED1+ microglia (brown, c) or GFAP+ astrocytes (brown, d). Scale bar?=?100 μm for (a, c) and d; 50 μm for (b)

From: Xu K, Yang Z, Shi R, Luo C, Zhang Z.
Expression of aryl hydrocarbon receptor in rat brain lesions following traumatic brain injury.
Diagn Pathol. 2016 Aug 9;11(1):72.

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CD68 Antibody | ED1 gallery image 39

Published customer image:
Mouse anti Rat CD68 (MCA341GA) used to identify microglia in nanowire neural implants in rat striatum by immunoflurescence.
Image caption:
Inflammatory tissue response, cell nuclei and neuronal density (inner ROI). Quantification in the inner ROI (0–50 μm) of ED1-positive area (a), GFAP-positive area (b), NeuN density (c), and cell nuclei density (d) at 1 year for HfOx-coated GaP nanowires (biostable) and SiOx-coated GaP nanowires (degradable). The boxes correspond to median values with indication of the 25 and 75 percentiles, and the whiskers show the minimum and maximum values. Mann–Whitney test was used. Representative fluorescent images of the tissue response 1 year post injection of e 2 μm long HfOx-coated nanowires (biostable) and f 2 μm long SiOx-coated GaP nanowires (degradable). ED1-positive cells (green), GFAP-positive cells (red) and cell nuclei (blue). Scale bar 100 μm.

From: Gällentoft L, Pettersson LM, Danielsen N, Schouenborg J, Prinz CN, Linsmeier CE.
Impact of degradable nanowires on long-term brain tissue responses.
J Nanobiotechnology. 2016 Aug 9;14(1):64.

Enlarge
CD68 Antibody | ED1 gallery image 40

Published customer image:
Mouse anti Rat CD68 (MCA341GA) used to identify microglia in nanowire neural implants in rat striatum by immunoflurescence.
Image caption:
Confocal images 1 year post nanowire injection. Representative laser scanning confocal microscopy images of the scar area after injection of 2 μm long HfOx-coated (a–e) and SiOx-coated GaP (f–j) nanowires 1 year post injection. The intact HfOx-coated and fragmented SiOx-coated GaP nanowires are visualized in white using scattered laser light mode. The images demonstrate the difference in nanowire cell load and degradability of the two types of nanowires studied. Note also the few rod-shaped SiOx-coated nanowires found in single, small ED1-positive cells (arrows). ED1-positive cells (green), GFAP-positive cells (red), cell nuclei (blue) and nanowires (white, scattered laser light). Scale bar 20 μm.

From: Gällentoft L, Pettersson LM, Danielsen N, Schouenborg J, Prinz CN, Linsmeier CE.
Impact of degradable nanowires on long-term brain tissue responses.
J Nanobiotechnology. 2016 Aug 9;14(1):64.

Enlarge
CD68 Antibody | ED1 gallery image 41

Published customer image:
Mouse anti Rat CD68 (MCA341GA) used to identify microglia in nanowire neural implants in rat striatum by immunoflurescence.
Image caption:
Close-up confocal images. Merged close-up of laser scanning confocal microscopy images showing ED1-positive cells in the scar area after injection of 2 μm long HfOx-coated (a) and SiOx-coated GaP (b) nanowires 1 year post injection. The images show internalization of intact HfOx-coated and degraded SiOx-coated GaP nanowires in microglia/macrophages. ED1-positive cells (green), GFAP-positive cells (red), cell nuclei (blue) and nanowires (white, scattered laser light). Scale bar 5 μm.

From: Gällentoft L, Pettersson LM, Danielsen N, Schouenborg J, Prinz CN, Linsmeier CE.
Impact of degradable nanowires on long-term brain tissue responses.
J Nanobiotechnology. 2016 Aug 9;14(1):64.

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  • Mouse anti Rat CD68:RPE
  • Mouse anti Rat CD68:Alexa Fluor® 700
  • Mouse anti Rat CD68
  • Mouse anti Rat CD68:Alexa Fluor® 488
  • Mouse anti Rat CD68:Biotin
  • Mouse anti Rat CD68:Alexa Fluor® 647
  • Mouse anti Rat CD68:FITC
  • Mouse anti Rat CD68
(Rated 5.0 out of 5 based on 1 customer reviews)
    Mouse anti rat CD68, clone ED1 recognizes the rat ED1 antigen. The ED1 antigen is expressed on most macrophages populations, as well as on monocytes and is considered as a pan-macrophage marker in the rat.
    • Product Type
      Monoclonal Antibody
    • Clone
      ED1
    • Isotype
      IgG1
    7 Formats Available
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      MCA341FF *datasheet pdfdatasheet pdf0.1 mg
      MCA341F
      MCA341GAC, F *, IF, IP, P *datasheet pdfdatasheet pdf0.1 mg
      MCA341GA
      MCA341RC, F *, IF, IP, P *, R, WBdatasheet pdfdatasheet pdf0.25 mg
      MCA341R
      MCA341PEF *datasheet pdfdatasheet pdf100 Tests
      MCA341PE
      MCA341A488F *datasheet pdfdatasheet pdf100 Tests
      MCA341A488
      MCA341BCdatasheet pdfdatasheet pdf100 Tests
      MCA341B
      MCA341A700F *datasheet pdfdatasheet pdf100 Tests/1ml
      MCA341A700
      MCA341A647F *datasheet pdfdatasheet pdf100 Tests/1ml
      MCA341A647
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      More Images
      References
      Reviews
      -
      • Mouse anti rat CD68, clone ED1 recognizes the rat ED1 antigen, a heavily glycosylated protein of 90-110 KDa, also known as rat CD68 (Dijkstra et al. 1985).

        The ED1 antigen is expressed on most macrophages populations, as well as on monocytes and is considered as a pan-macrophage marker in the rat (Damoiseaux et al. 1994). ED1 is expressed predominantly on the lysosomal membrane and lightly on the cell surface (Dijkstra et al. 1985).

        The expression of ED1 antigen being predominantly cytoplasmic (Dijkstra et al. 1985), flow cytometry results are improved by the use of a membrane permeabilization procedure, such as Leucoperm, prior to staining.
      • Intended Use
      • Target Species
        Rat
      • Species Cross-Reactivity
        Target SpeciesCross Reactivity
        Bovineyes
        Horseno
        N.B. Antibody reactivity and working conditions may vary between species.
      • Product Form
        Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
        Purified IgG conjugated to Alexa Fluor® 700 - liquid
        Purified IgG - liquid
        Purified IgG conjugated to Alexa Fluor® 488 - liquid
        Purified IgG conjugated to Biotin - liquid
        Purified IgG conjugated to Alexa Fluor® 647 - liquid
        Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
        Purified IgG - liquid
      • Reconstitution
        Reconstitute with 1 ml distilled water
      • Preparation
        Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
      • Preservative Stabilisers
        0.09%Sodium Azide
        1%Bovine Serum Albumin
        0.09%Sodium Azide
        1%Bovine Serum Albumin
        Pack Size: 0.1 mg
        0.09%Sodium Azide
        Pack Size: 0.25 mg0.09% sodium azide.
        0.09%Sodium Azide
        1%Bovine Serum Albumin
        0.09%Sodium Azide
        1%Bovine Serum Albumin
        0.09%Sodium Azide
        1%Bovine Serum Albumin
        0.09%Sodium Azide
        1%Bovine Serum Albumin
        Pack Size: 0.1 mg
        0.09%Sodium Azide
        Pack Size: 0.25 mg0.09% sodium azide.
      • Immunogen
        Rat spleen cells
      • Purity
      • Approx. Protein Concentrations
        IgG concentration 0.05 mg/ml
        Pack Size: 0.1 mgIgG concentration 0.5 mg/ml
        Pack Size: 0.25 mgIgG concentration 1.0 mg/ml
        IgG concentration 0.05 mg/ml
        IgG concentration 0.1 mg/ml
        IgG concentration 0.05 mg/ml
        IgG concentration 0.1 mg/ml
        Pack Size: 0.1 mgIgG concentration 0.5 mg/ml
        Pack Size: 0.25 mgIgG concentration 1.0 mg/ml
      • Reagents In The Kit
      • Preparing The Antibody
      • Test Principle
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
      • Fusion Partners
        Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0-Ag14 mouse myeloma cell line.
      • Storage
        Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

        This product should be stored undiluted.

        DO NOT FREEZE. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

        Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

        Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

        Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

        Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      • Shelf Life
        12 months from date of reconstitution.
        18 months from date of despatch.
        18 months from date of despatch.
        18 months from date of despatch.
        18 months from date of despatch.
        18 months from date of despatch.
        18 months from date of despatch.
        18 months from date of despatch.
      • Acknowledgements
        This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
        This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
        This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
      • Regulatory
        For research purposes only
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry(1)Neat1/10
        (1)
        Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry(1)Neat
        (1)
        Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
      • Application NameYesNoMin DilutionMax Dilution
        Immunofluorescence
        Immunohistology - Frozen
        Immunoprecipitation
        Flow Cytometry(1)1/501/100
        Immunohistology - Paraffin(2)1/100
        Radioimmunoassays
        Western Blotting
        (1)
        Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
        (2)
        This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry(1)Neat
        (1)
        Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
      • Application NameYesNoMin DilutionMax Dilution
        Immunohistology - Frozen1/10
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry(1)Neat
        (1)
        Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry(1)Neat
        (1)
        Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
      • Application NameYesNoMin DilutionMax Dilution
        Immunofluorescence
        Immunohistology - Frozen
        Immunoprecipitation
        Flow Cytometry(1)1/501/100
        Immunohistology - Paraffin(2)1/100
        Radioimmunoassays
        Western Blotting
        (1)
        Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
        (2)
        This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase

      • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Flow Cytometry
        Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
        This clone is suitable for use in western blotting applications. Bio-Rad recommend MCA341R for this purpose.
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use

      Additional CD68 Antibody Formats

      Formats Clone Applications Sizes available
      CD68 Antibody : FITC ED1 F * 0.1 mg
      CD68 Antibody : Alexa Fluor® 647 ED1 F * 100 Tests/1ml
      CD68 Antibody : Alexa Fluor® 488 ED1 F * 100 Tests
      CD68 Antibody : RPE ED1 F * 100 Tests
      CD68 Antibody : Purified ED1 C, F *, IF, IP, P *, R, WB 0.1 mg | 0.25 mg
      CD68 Antibody : Biotin ED1 C 100 Tests
      CD68 Antibody : Alexa Fluor® 700 ED1 F * 100 Tests/1ml
      • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

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          Mouse IgG1 Negative Control:Alexa Fluor® 488MCA1209A488100 Tests/1mlF
          MCA1209A488
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse IgG1 Negative Control:Alexa Fluor® 647MCA1209A647100 Tests/1mlF
          MCA1209A647
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse IgG1 Negative Control:FITCMCA1209F0.1 mgF
          MCA1209F
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse IgG1 Negative ControlMCA12090.1 mgF
          MCA1209

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

                Source Reference

                References

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                Submitted: 30 Aug 2014

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