CD172a Antibody | OX-41

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CD172a Antibody | OX-41 gallery image 1

Published clone specific image
Alloimmunity-associated cytotoxicity is mediated through the NKG2D receptor. (A) Liver expression of nkg2d on day ten after liver transplantation. (B) Representative NKG2D expression levels in blood NK cells (left) and monocytes (right) of allogeneic (black) and syngeneic (grey) recipients. Isotype was used as control (dashed lines). (C) Sorted blood NK cell cytotoxicity inhibition with anti-NKG2D antibody or with anti-NKp30 antibody. (D) Levels of NKG2D ligand (rae1l, rrlt and irp94) expression in the liver on day ten after transplantation. (E) Levels of NKG2D ligand (rae1l, rrlt and irp94) expression in rat HCC cell lines. (F) Representative level of recombinant NKG2D-Fc binding to rat HCC cells lines. *p<0.05, **<0.01.

From: Lacotte S, Oldani G, Slits F, Orci LA, Rubbia-Brandt L, et al. (2014) Alloimmune Activation Promotes Anti-Cancer Cytotoxicity after Rat Liver Transplantation. PLoS ONE 9(3): e91515.

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CD172a Antibody | OX-41 gallery image 2

Immunofluorescence staining of rat lymphnode cryosection with Mouse anti Rat CD172a antibody (MCA273), red in A and Mouse anti Rat CD4 (MCA55), green in B. C is the Merged image with nuclei counter-stained blue using DAPI. Low power

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CD172a Antibody | OX-41 gallery image 3

Immunofluorescence staining of rat lymphnode cryosection with Mouse anti Rat CD172a antibody (MCA273), red in A and Mouse anti Rat CD4 (MCA55), green in B. C is the Merged image with nuclei counter-stained blue using DAPI. Medium power

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CD172a Antibody | OX-41 gallery image 4

Immunofluorescence staining of rat lymphnode cryosection with Mouse anti Rat CD172a antibody (MCA273), red in A and Mouse anti Rat CD4 (MCA55), green in B. C is the Merged image with nuclei counter-stained blue using DAPI. High power

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CD172a Antibody | OX-41 gallery image 5

Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD172a antibody (MCA274) followed by horseradish peroxidase conjugated Goat anti Mouse IgG2a (STAR133P) as a detection reagent. Low power

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CD172a Antibody | OX-41 gallery image 6

Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD172a antibody (MCA274) followed by horseradish peroxidase conjugated Goat anti Mouse IgG2a (STAR133P) as a detection reagent. Medium power

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CD172a Antibody | OX-41 gallery image 7

Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD172a antibody (MCA274) followed by horseradish peroxidase conjugated Goat anti Mouse IgG2a (STAR133P) as a detection reagent. High power

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  • Mouse anti Rat CD172a:RPE
  • Mouse anti Rat CD172a:FITC
  • Mouse anti Rat CD172a
  • Mouse anti Rat CD172a
  • Mouse anti Rat CD172a
  • Mouse anti Rat CD172a:Biotin
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    OX-41
  • Isotype
    IgG2a
4 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA274FFdatasheet pdfdatasheet pdf0.1 mg
    MCA274F
    MCA274GAC, F, P *datasheet pdfdatasheet pdf0.1 mg
    MCA274GA
    MCA274BC, F, P *datasheet pdfdatasheet pdf0.1 mg
    MCA274B
    MCA274RC, F, P *, WBdatasheet pdfdatasheet pdf0.25 mg
    MCA274R
    MCA274GC, F, P *, WBdatasheet pdfdatasheet pdf1 mg
    MCA274G
    MCA274PEFdatasheet pdfdatasheet pdf100 Tests
    MCA274PE
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Rat CD172a antibody, clone OX-41 recognizes rat CD172a, also known as Signal regulatory protein (SIRP). CD172a is expressed selectively by myeloid cells and by neurons.

      MRC OX-41 binds to a different epitope on SIRP than ED9. It does not block the interaction of CD172a - CD47.
    • Intended Use
    • Target Species
      Rat
    • Product Form
      Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG - liquid
      Purified IgG - liquid
      Purified IgG - liquid
      Purified IgG conjugated to Biotin - liquid
    • Reconstitution
      Reconstitute with 1 ml distilled water
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide
      1.0%Bovine Serum Albumin
      5%Sucrose
      0.09%Sodium Azide
      1.0%Bovine Serum Albumin
      0.09%Sodium Azide
      0.09%Sodium Azide
      0.09%Sodium Azide
      0.09%Sodium Azide
      1.0%Bovine Serum Albumin
    • Immunogen
      Rat peritoneal macrophages.
    • Purity
    • Approx. Protein Concentrations
      Isotype concentration 0.1 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 0.1 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised BALB/c mice were fused with the NS0/U mouse myeloma cell line.
    • Storage
      Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of reconstitution.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
      1/100 Pack Size: 1 mg, 0.1 mg
      1/10 Pack Size: 0.25 mg
      1/200Pack Size: 1 mg, 0.1 mg
      1/50Pack Size: 0.25 mg
      Immunohistology - Frozen
      Western Blotting
      Immunohistology - Paraffin(1)
      (1)
      This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
      1/100 Pack Size: 1 mg, 0.1 mg
      1/10 Pack Size: 0.25 mg
      1/200Pack Size: 1 mg, 0.1 mg
      1/50Pack Size: 0.25 mg
      Immunohistology - Frozen
      Western Blotting
      Immunohistology - Paraffin(1)
      (1)
      This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
      1/100 Pack Size: 1 mg, 0.1 mg
      1/10 Pack Size: 0.25 mg
      1/200Pack Size: 1 mg, 0.1 mg
      1/50Pack Size: 0.25 mg
      Immunohistology - Frozen
      Western Blotting
      Immunohistology - Paraffin(1)
      (1)
      This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
      Immunohistology - Frozen
      Immunohistology - Paraffin(1)
      (1)
      This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional CD172a Antibody Formats

    Formats Clone Applications Sizes available
    CD172a Antibody : FITC OX-41 F 0.1 mg
    CD172a Antibody : Purified OX-41 C, F, P *, WB 1 mg | 0.1 mg | 0.25 mg
    CD172a Antibody : RPE OX-41 F 100 Tests
    CD172a Antibody : Biotin OX-41 C, F, P * 0.1 mg
    • Copyright © 2016 Bio-Rad

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              References

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