CD11b Antibody | ED8

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CD11b Antibody | ED8 gallery image 1

Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD11 antibody, clone ED8 (MCA619) followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 (STAR132P) as a detection reagent. Low power

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CD11b Antibody | ED8 gallery image 2

Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD11 antibody, clone ED8 (MCA619) followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 (STAR132P) as a detection reagent. Medium power

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CD11b Antibody | ED8 gallery image 3

Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD11 antibody, clone ED8 (MCA619) followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 (STAR132P) as a detection reagent. High power

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CD11b Antibody | ED8 gallery image 4

Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD11b antibody, clone ED8 (MCA619), red in A and CD4 (MCA55), green in B. C is the merged image with nuclei counterstained blue using DAPI. Low power

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CD11b Antibody | ED8 gallery image 5

Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD11b antibody, clone ED8 (MCA619), red in A and CD4 (MCA55), green in B. C is the merged image with nuclei counterstained blue using DAPI. High power

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CD11b Antibody | ED8 gallery image 6

Published customer image:
Lithium inhibits lipopolysaccharide (LPS)-induced microglial activation. Primary microglial cells were pretreated with LiCl at 0.3, 1 and 3 mM for 30 minutes and then stimulated with LPS at 10 ng/ml for 24 hours. (a) and (b) For flow cytometric analysis, the cells were incubated with PE-conjugated ED8 antibody at 37°C for 1 hour. **P <0.01 versus the response to medium alone. #P <0.05, ##P <0.01 versus LPS treatment groups. The data are presented as the mean?±?s.e.m. of three independent experiments. (c) The cells were stained with ED8 antibody and upregulated ED8-immunopositive expression (green) on the activated microglia was observed using confocal scanning. The blue staining represents DAPI. Scale bar 50 µm.

From: Dong H, Zhang X, Dai X, Lu S, Gui B, Jin W, Zhang S, Zhang S, Qian Y. Lithium ameliorates lipopolysaccharide-induced microglial activation via inhibition of toll-like receptor 4 expression by activating the PI3K/Akt/FoxO1 pathway. J Neuroinflammation. 2014 Aug 14;11:140.

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CD11b Antibody | ED8 gallery image 7

Published customer image:
TLR4 regulates microglial activation. Primary microglial cells were transfected with TLR4 siRNA (100 nM) or control (siControl) for 48 hours and then stimulated with lipopolysaccharide (LPS) (10 ng/ml) for 24 hours. (a) Suppression of TLR4 expression by TLR4 siRNA. (b) Quantification of IL-6 and TNF-a production in media, **P <0.01 versus the response to medium alone. ##P <0.01 versus LPS treatment groups. The data are presented as the mean?±?s.e.m. of three independent experiments. (c) The cells were stained with ED8 antibody and upregulated ED8-immunopositive expression (green) on activated microglia was observed using confocal. The blue staining represents DAPI. Scale bar 50 µm.

From: Dong H, Zhang X, Dai X, Lu S, Gui B, Jin W, Zhang S, Zhang S, Qian Y. Lithium ameliorates lipopolysaccharide-induced microglial activation via inhibition of toll-like receptor 4 expression by activating the PI3K/Akt/FoxO1 pathway. J Neuroinflammation. 2014 Aug 14;11:140.

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CD11b Antibody | ED8 gallery image 8

Published customer image:
Mouse anti Rat CD11b antibody, clone ED8 used for the evaluation of CD11b expression on spinal cord mononuclear cells by flow cytometry.
Image caption:
Aging increases the number of all spinal cord mononuclear cells, and CD45 int/hi CD11b?+?nonlymphoid cells, recovered on the 7 th d.p.i. from AO rat spinal cord. (a) Bar graph indicates the total number of mononuclear cells (MNC) isolated from spinal cord of young and aged rats on the 7 th d.p.i. (Panel b) Flow cytometry density plots indicate the expression of CD45 on CD11b?+?cells from spinal cord of (left) young and (right) aged rats on the 7 th d.p.i. CD11b?+?cells were gated as shown in the flow cytometry histograms. Numbers in the flow cytometry histograms represent the percentage of CD11b?+?cells. R1?=?CD11b?+?CD45 hi cells; R2?=?CD11b?+?CD45 int cells. Numbers in the table indicate the percentage of cells in the specified region (R). Bar graph indicates the number of CD11b?+?cells in spinal cords from young and aged rats. (Panel c) Bar graphs indicate the number of (left) CD11b?+?CD45 int and (right) CD11b?+?CD45 hi cells in spinal cords from young and aged rats on the 7 th d.p.i. (d) Bar graph indicates the fold change in expression of mRNA for SOCS1 in spinal cord mononuclear cells from aged relative to young rats on the 7 th d.p.i. as determined by RT-qPCR. Data are normalized to β-actin (ACTB). All results are presented as means?±?SEM (n?=?9/group). Data are representative of one of two experiments with similar results. *p?
From: Stojić-Vukanić Z, Nacka-Aleksić M, Pilipović I, Vujnović I, Blagojević V, Kosec D, Dimitrijević M, Leposavić G. Aging diminishes the resistance of AO rats to EAE: putative role of enhanced generation of GM-CSF Expressing CD4+ T cells in aged rats. Immun Ageing. 2015 Oct 6;12:16.

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  • Mouse anti Rat CD11b
  • Mouse anti Rat CD11b:RPE
  • Mouse anti Rat CD11b:FITC
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  • Product Type
    Monoclonal Antibody
  • Clone
    ED8
  • Isotype
    IgG1
3 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA619RC *datasheet pdfdatasheet pdf0.25 mg
    MCA619R
    MCA619PEFdatasheet pdfdatasheet pdf100 Tests
    MCA619PE
    MCA619FFdatasheet pdfdatasheet pdf100 Tests
    MCA619F
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Rat CD11b antibody, clone ED8 recognizes a membrane antigen on rat macrophages, monocytes, dendritic cells and granulocytes. It also recognizes small ramified microglia in the central nervous system. No other cell types are positive for ED8. The recognized antigen is a heterodimer consisting of ~160 and ~95 kDa, belonging to the family of adhesion molecules CD11b/CD18, also designated as Mac-1 antigen or CR3. Mouse anti Rat CD11b antibody, clones ED7 (MCA618R) and ED8 may recognize closely related epitopes on the same molecule. Both clones ED7 and ED8 induce homotypic aggregation of granulocytes (Drasković-Pavlović et al. 1999).
    • Intended Use
    • Target Species
      Rat
    • Product Form
      Purified IgG - liquid
      Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
    • Reconstitution
      Reconstitute with 1 ml distilled water
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by ion exchange chromatography
      Purified IgG prepared by ion exchange chromatography
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      5%Sucrose
      0.09%Sodium Azide
      1%Bovine Serum Albumin
    • Immunogen
      Rat spleen cell homogenate with Freund's complete adjuvant.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
      IgG concentration 0.1 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised BAL/B/c mice were fused with cells of the Sp2/0 Ag-14 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      12 months from date of reconstitution.
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Paraffin
      Immunohistology - Frozen(1)
      (1)
      The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
      Immunohistology - Paraffin

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional CD11b Antibody Formats

    Formats Clone Applications Sizes available
    CD11b Antibody : RPE ED8 F 100 Tests
    CD11b Antibody : Purified ED8 C * 0.25 mg
    CD11b Antibody : FITC ED8 F 100 Tests
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117A0.5 mgE, WB
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      Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GA0.1 mgF, IF
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      STAR117D649GA
      Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)STAR117D680GA0.1 mgF, WB
      STAR117D680GA
      Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GA0.1 mgF, IF, WB
      STAR117D800GA
      Rabbit F(ab')2 anti Mouse IgG:Dylight®800STAR8D800GA0.1 mgF, IF, WB
      STAR8D800GA
      Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
      STAR117F
      Goat anti Mouse IgG:FITC (Rat Adsorbed)STAR700.5 mgF
      STAR70
      Goat anti Mouse IgG (Fc):FITCSTAR120F1 mgC, F
      STAR120F
      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
      STAR9B
      Human anti Mouse IgG1:HRPHCA036P0.1 mgE
      HCA036P
      Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117P0.5 mgE, WB
      STAR117P
      Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
      STAR77
      Goat anti Mouse IgG (Fc):HRPSTAR120P1 mgE, WB
      STAR120P
      Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
      STAR13B
      Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87P1 mgE
      STAR87P
      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
      STAR12A
      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF
      STAR76

      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative Control:RPEMCA1209PE100 TestsF
        MCA1209PE
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative Control:FITCMCA1209F0.1 mgF
        MCA1209F

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Functional Assays

              References

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