Ranibizumab antibody | AbD29858_hIgG1
A microtiter plate was coated overnight with various antigens at a concentration of 5 μg/ml. After washing and blocking with PBST+5% BSA, detection was performed using HRP conjugated Human Anti-Ranibizumab Antibody, clone AbD29858_hIgG1 (HCA316P) at a concentration of 2 μg/ml in HISPEC Assay Diluent (BUF049A) followed by QuantaBlu Fluorogenic Peroxidase Substrate.
A microtiter plate was coated overnight with recombinant human VEGF-A (PHP293), ranibizumab or human Fab VH3-23/kappa1 at a concentration of 5 µg/ml. After washing and blocking with PBST+5% BSA, the human VEGF-A/ranibizumab complex was formed by adding 2 µg/ml ranibizumab to the wells coated with VEGF-A. Detection was performed using HRP conjugated Human Anti-Ranibizumab Antibody, clone AbD29858_hIgG1 (HCA316P) titrated to the given concentrations in HISPEC Assay Diluent (BUF049A) followed by QuantaBlu Fluorogenic Peroxidase Substrate. Data are shown as the mean of three measurements.
A microtiter plate was coated overnight with recombinant human VEGF-A (PHP293) at a concentration of 5 µg/ml. After washing and blocking with PBST+5% BSA, 10% human serum was added spiked with increasing concentrations of ranibizumab. Detection was performed using HRP conjugated Human Anti-Ranibizumab Antibody, clone AbD29858_hIgG1 (HCA316P) at a concentration of 2 µg/ml in HISPEC Assay Diluent (BUF049A) followed by QuantaBlu Fluorogenic Peroxidase Substrate. Data are shown as the mean of three measurements.
Filter by Application:
E ResetHuman anti Ranibizumab:HRP
- Product Type
- Monoclonal Antibody
- Clone
- AbD29858_hIgG1
- Isotype
- IgG1
- Specificity
- Ranibizumab
Antibody Quality and Performance Guarantee
| Human Anti-Ranibizumab Antibody, clone AbD29858_hIgG1 is a non-inhibitory, anti-idiotypic antibody in full immunoglobulin format that specifically recognizes the monoclonal antibody drug ranibizumab and ranibizumab when in complex with recombinant human vascular endothelial growth factor A (VEGF-A). It does not recognize free human VEGF-A or bevacizumab. This antibody can be used to measure levels of free ranibizumab and biosimilars in patient samples. Clone AbD29858_hIgG1 in full immunoglobulin format can be used to develop a pharmacokinetic (PK) antigen capture format assay to measure free ranibizumab that has been captured via immobilized VEGF-A. Ranibizumab (Lucentis) is a humanized Fab fragment antibody (VH3-23/kappa1), originating from the same parental mouse antibody as bevacizumab (Avastin); it has 4 amino acid exchanges in the heavy chain and 1 amino acid exchange in the light chain compared to bevacizumab. Ranibizumab was first approved to treat wet age-related macular degeneration (AMD), a common form of age-related vision loss. It is also approved for the treatment of macular edema following retinal vein occlusion, diabetic macular edema, and diabetic retinopathy. Ranibizumab inhibits angiogenesis by binding to VEGF-A, blocking its interaction with VEGF receptors on the surface of endothelial cells, and thus preventing the subsequent growth of new blood vessels. View a summary of all anti-ranibizumab antibodies |
- Product Form
- Human IgG1 antibody (lambda light chain) selected from the HuCAL phage display library and expressed in a human cell line. This antibody is conjugated to horseradish peroxidase (HRP) and supplied as liquid.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Source
- HKB-11
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Thiomersal
- Immunogen
- Ranibizumab
- Affinity
- The intrinsic affinity of Human anti Ranibizumab antibody, clone AbD29858_hIgG1 is KD=18 nM as measured by real time, label-free molecular interaction analysis on immobilized VEGF/Ranibizumab complex.
- Approx. Protein Concentrations
- Antibody concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
Lucentis is a trademark of Genentech.
This product is shipped frozen.
When ready to use, thaw and aliquot the sample as needed. Store aliquots at -70°C, if available, otherwise store at -20°C. It is not recommended to keep aliquots at 4°C for more than one week.
When ready to use, thaw and aliquot the sample as needed. Store aliquots at -70°C, if available, otherwise store at -20°C. It is not recommended to keep aliquots at 4°C for more than one week.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.
- ELISA
- Human anti Ranibizumab antibody, clone AbD29858_hIgG1 can be used in a direct or indirect ELISA on Ranibizumab or as a detection reagent for free Ranibizumab captured via immobilized VEGF-A.
Protocol: PK antigen capture ELISA to measure free drug.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Recombinant Human IgG1 Lambda Allotype G1m3 | HCA049 | E | 0.1 mg |
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| List Price | Your Price | ||||||
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| Description | Recombinant Human IgG1 Lambda Allotype G1m3 | ||||||
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Hispec Assay Diluent | BUF049A | E IY | 50 ml | Log in | |||
| List Price | Your Price | ||||||
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| Description | Hispec Assay Diluent | ||||||
| Recombinant Human VEGF-A | PHP293 | E | 0.2 mg |
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| List Price | Your Price | ||||||
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| Description | Recombinant Human VEGF-A | ||||||
- Synonyms
- Lucentis
- Licensed Use
- For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
View more products with Ranibizumab specificity
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