CD14 Antibody | MIL2

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CD14 Antibody | MIL2 gallery image 1

Staining of pig peripheral blood granulocytes with Mouse anti Pig CD14 (MCA1218GA) followed by Goat anti Mouse IgG:FITC (STAR117F)

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CD14 Antibody | MIL2 gallery image 2

Published customer image:
Mouse anti Pig CD14 antibody, clone MIL2 used for the evaluation of CD14 expression on porcine leukocytes by flow cytometry.
Image caption:
Overview of flow cytometry analyses. (A) Gating strategy for identification of monocytes and macrophages. The peripheral blood is shown. Designation of population shown within each dot-plot is indicated above the dot-plot. Leukocytes were identified as viable (a) non-doublet (b) cells with typical light scatter properties of leukocytes (c). Then, macrophages were gated simply as CD203ahi leukocytes (d) and marked with blue color. Monocytes were gated as CD203alow/- SWC8-(e) CD172ahi(f) leukocytes where the CD203alow/- region was defined as the complementary region to the CD203ahi region. Then, SLA-DR+ monocytes were marked with red color and SLA-DR- monocytes were marked with green color (g). SLA-DR- region was defined as the complementary region to the SLA-DR+ region. Gating order is shown in the scheme (h). (B) Representative CD163 vs. CD14 dot-plots of macrophages (blue) and monocyte subpopulations (green: SLA-DR–, red: SLA-DR+) in various body compartments of control and APP-infected pigs.

From: Ondrackova P, Leva L, Kucerova Z, Vicenova M, Mensikova M, Faldyna M. Distribution of porcine monocytes in different lymphoid tissues and the lungs during experimental Actinobacillus pleuropneumoniae infection and the role of chemokines.
Vet Res. 2013 Oct 17;44:98.

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CD14 Antibody | MIL2 gallery image 3

Published customer image:
Mouse anti Pig CD14, clone MIL2 used for the separation of mococytes from porcine PBMCs by immunomagnetic cell sorting.
Image caption:
Porcine MoDCs at different culture times (400×).CD14+ monocytes were purified from porcine PBMCs and were cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS), 25 ng/mL recombinant porcine IL-4, and 10 ng/ml recombinant porcine GM-CSF. The culture medium was replenished every 3 days. (A) CD14+ monocytes after 2 days of culture. (B) CD14+ monocytes 3 days of culture. (C) CD14+ monocytes after 4 days of culture. (D) CD14+ monocytes after 5 days of culture.

From: Citation: Liu J, Tian Z-Y, Xiao Y-C, Wang X-L, Jin M-L, Shi D-S (2016) The Role of Porcine Monocyte Derived Dendritic Cells (MoDC) in the Inflammation Storm Caused by Streptococcus suis Serotype 2 Infection. PLoS ONE 11(3): e0151256.

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CD14 Antibody | MIL2 gallery image 4

Figure A. FITC conjugated mouse anti porcine CD14 (MCA1218F) and A647 conjugated mouse IgG1 isotype control (MCA928A647). Figure B. FITC conjugated mouse anti porcine CD14 (MCA1218F) and A647 conjugated mouse anti porcine CD45 (MCA1222A647). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.

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CD14 Antibody | MIL2 gallery image 5

Figure A. A647 conjugated mouse anti porcine CD45 (MCA1222A647) and FITC conjugated mouse IgG2b isotype control (MCA691F). Figure B. A647 conjugated mouse anti porcine CD45 (MCA1222A647) and FITC conjugated mouse anti porcine CD14 (MCA1218F). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.

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  • Mouse anti Pig CD14:FITC
  • Mouse anti Pig CD14
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    MIL2
  • Isotype
    IgG2b
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA1218FFdatasheet pdfdatasheet pdf0.1 mg
    MCA1218F
    MCA1218GAC, F, IFdatasheet pdfdatasheet pdf0.1 mg
    MCA1218GA
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Pig CD14, clone MIL2 recognizes porcine CD14. Clone MIL2 was clustered as porcine CD14 at the Third International Workshop on Swine Leukocyte Differentiation Antigens (Haverson et al. 2001) . Clone MIL2 immunoprecipitates a protein of ~50 kDa consistent with the expected apparent molecular weight of porcine CD14, and demonstrates the expected CD14 profile by dual labelling and competition studies. Further, pre-incubation of peripheral blood monocytes with MIL2 inhibits the binding of FITC labelled LPS, consistent with masking the CD14 LPS binding site (Thacker et al. 2001) .

      Mouse anti pig CD14, clone MIL2 demonstrates staining of both monocytes and neutrophils in peripheral blood by flow cytometry with a similar expression pattern to the anti human CD14 clone TüK4, lymphocytes and eosinophils are negative for MIL2 staining (Zelnickova et al. 2007). Cloning and characterization of porcine CD14 indicates a high degree of both functional and structural conservation when compared to CD14 from other mammalian species, the gene maps to chromosome 2 and is expressed on a wide range of tissues in a manner consistent with expression on myeloid cells. (Petersen et al. 2007, Sanz et al. 2007).
    • Intended Use
    • Target Species
      Pig
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Humanyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide (NaN3)
      1%Bovine Serum Albumin
      0.09% Sodium Azide (NaN3)
    • Immunogen
      Porcine peripheral blood lymphocytes.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.1mg/ml.
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunized Balb/c mice were fused with cells from the P2-X63-Ag.653 mouse myeloma.
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted.
      This product is photosensitive and should be protected from light.
      Avoid repeated freezing and thawing as this may denature the antibody.
      Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/251/200
      Immunofluorescence
      Immunohistology - Frozen

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional CD14 Antibody Formats

    Formats Clone Applications Sizes available
    CD14 Antibody : FITC MIL2 F 0.1 mg
    CD14 Antibody : Purified MIL2 C, F, IF 0.1 mg
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117A0.5 mgE, WB
      STAR117A
      Goat anti Mouse IgG/A/M:Alk. Phos.STAR87A1 mgC, E, WB
      STAR87A
      Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GA0.1 mgF, IF
      STAR117D488GA
      Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)STAR117D549GA0.1 mgF, IF
      STAR117D549GA
      Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)STAR117D649GA0.1 mgF, IF
      STAR117D649GA
      Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)STAR117D680GA0.1 mgF, WB
      STAR117D680GA
      Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GA0.1 mgF, IF, WB
      STAR117D800GA
      Rabbit F(ab')2 anti Mouse IgG:Dylight®800STAR8D800GA0.1 mgF, IF, WB
      STAR8D800GA
      Human anti Mouse IgG2b:FITCHCA038F0.1 mgF
      HCA038F
      Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
      STAR117F
      Goat anti Mouse IgG:FITC (Rat Adsorbed)STAR700.5 mgF
      STAR70
      Goat anti Mouse IgG (Fc):FITCSTAR120F1 mgC, F
      STAR120F
      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
      STAR9B
      Human anti Mouse IgG2b:HRPHCA038P0.1 mgE
      HCA038P
      Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117P0.5 mgE, WB
      STAR117P
      Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
      STAR77
      Goat anti Mouse IgG (Fc):HRPSTAR120P1 mgE, WB
      STAR120P
      Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
      STAR13B
      Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87P1 mgE
      STAR87P
      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
      STAR12A
      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF
      STAR76
      Human anti Mouse IgG2b:RPEHCA038PE100 TestsF
      HCA038PE

      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG2b Negative Control:FITCMCA691F100 TestsF
        MCA691F
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG2b Negative ControlMCA691100 TestsF
        MCA691

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              Source Reference

              References

              Further Reading

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