CD44 Antibody | 5035-41.1D

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CD44 Antibody | 5035-41.1D gallery image 1

Figure A. RPE conjugated rat anti mouse CD3 (MCA500PE) and FITC conjugated mouse IgG1 isotype control (MCA1209F). Figure B. RPE conjugated rat anti mouse CD3 (MCA500PE) and FITC conjugated mouse anti mouse CD44 (MCA1014F). All experiments performed on red cell lysed murine splenocytes gated on lymphocytes in the presence of murine SeroBlock (BUF041A).

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CD44 Antibody | 5035-41.1D gallery image 2

Figure A. APC conjugated rat anti mouse CD3 (MCA500APC) and FITC conjugated mouse IgG1 isotype control (MCA1209F). Figure B. APC conjugated rat anti mouse CD3 (MCA500APC) and FITC conjugated mouse anti mouse CD44 (MCA1014F). All experiments performed on murine splenocytes in the presence of murine SeroBlock (BUF041A).

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  • CD44 Antibody | 5035-41.1D thumbnail image 1
  • CD44 Antibody | 5035-41.1D thumbnail image 2
  • Mouse anti Mouse CD44
  • Mouse anti Mouse CD44:FITC
  • Mouse anti Mouse CD44
  • Mouse anti Mouse CD44:FITC
  • Mouse anti Mouse CD44:FITC
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    5035-41.1D
  • Isotype
    IgG1
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA1014FFdatasheet pdfdatasheet pdf0.1 mg
    MCA1014F
    MCA1014GF, IPdatasheet pdfdatasheet pdf0.25 mg
    MCA1014G
    MCA1014FBFdatasheet pdfdatasheet pdf0.5 mg
    MCA1014FB
    MCA1014FTFdatasheet pdfdatasheet pdf25 µg
    MCA1014FT
    MCA1014GTF, IPdatasheet pdfdatasheet pdf25 µg
    MCA1014GT
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Ly24.2 found on >90% bone marrow cells and spleen cells, 56% of thymus cells and 90% of lymph node cells.

      This antibody recognises the following strains of mice:- C57BL/6, C57BL/10, C57/L, C58A, AKR, 129, SJL, NZB, C3H, CE, CBA/H.
    • Intended Use
    • Target Species
      Mouse
    • Product Form
      Purified IgG - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
    • Immunogen
      Spleen cells from B6PL-Ly-2a / Ly-3a B6-Ly-1a mice
    • Purity
    • Approx. Protein Concentrations
      Pack Size: 0.25 mgIgG concentration 1.0 mg/ml
      Pack Size: 0.5 mgIgG concentration 0.5 mg/ml
      Pack Size: 25 µg, 0.1 mgIgG concentration 0.1mg/ml
      Pack Size: 0.25 mgIgG concentration 1.0 mg/ml
      Pack Size: 0.5 mgIgG concentration 0.5 mg/ml
      Pack Size: 25 µg, 0.1 mgIgG concentration 0.1mg/ml
      Pack Size: 0.5 mgIgG concentration 0.5 mg/ml
      Pack Size: 25 µg, 0.1 mgIgG concentration 0.1mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised mice were fused with cells of the mouse P3-NS1-1-Ag4 myeloma cell line.
    • Storage
      Store at +4oC for one month or at -20oC for longer.

      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC for one month or at -20oC for longer.

      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/501/100
      Immunoprecipitation
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/10
      NeatPack Size: 0.1 mg, 25 µg
      1/50Pack Size: 0.5 mg
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/501/100
      Immunoprecipitation
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/10
      NeatPack Size: 0.1 mg, 25 µg
      1/50Pack Size: 0.5 mg
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/10
      NeatPack Size: 0.1 mg, 25 µg
      1/50Pack Size: 0.5 mg

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional CD44 Antibody Formats

    Formats Clone Applications Sizes available
    CD44 Antibody : FITC 5035-41.1D F 0.5 mg | 25 µg | 0.1 mg
    CD44 Antibody : Purified 5035-41.1D F, IP 25 µg | 0.25 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse Seroblock FcRBUF041A0.1 mgF
          BUF041A
          Mouse Seroblock FcRBUF041B0.5 mgF
          BUF041B

          Recommended Positive Controls

            Histology Controls

              References

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