v-myb/c-myb antibody | 10A3
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Mouse anti v-myb/c-myb
- Product Type
- Monoclonal Antibody
- Clone
- 10A3
- Isotype
- IgG2a
- Specificity
- v-myb/c-myb
Antibody Quality and 
Unrivaled 
| Mouse anti v-myb/c-myb antibody, clone 10A3 recognizes both v-myb and c-myb. Myb is a transcription factor related to Myc, and is expressed primarily in haematopoietic cells and immature gastroepithelial cells. Mouse anti v-myb/c-myb antibody, clone 10A3 does not recognise xenopus A-myb. |
- Target Species
- Xenopus
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Human Zebrafish - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- Recombinant v-myb protein, DNA binding protein.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised B110x Balb/c F1 mice were fused with cells of the mouse SP2/0 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunoprecipitation |  |
||
| Western Blotting | |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
References for v-myb/c-myb antibody
-
Sleeman, J.P. (1993) Xenopus A-myb is expressed during early spermatogenesis.
Oncogene. 8 (7): 1931-41. -
Grabher, C. et al. (2011) Zebrafish microRNA-126 determines hematopoietic cell fate through c-Myb.
Leukemia. 25 (3): 506-14.
- RRID
- AB_1125374
- UniProt
- Q08759
- Entrez Gene
- myb
- GO Terms
- GO:0003677 DNA binding
- GO:0005634 nucleus
- GO:0006350 transcription
- GO:0030528 transcription regulator activity
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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