SARS-CoV Nucleoprotein antibody | 3851

Mouse anti SARS-CoV Nucleoprotein

Product Type
Monoclonal Antibody
Clone
3851
Isotype
IgG2b
Specificity
SARS-CoV Nucleoprotein

Product Code Applications Pack Size List Price Your Price Qty
MCA6373
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E WB 0.1 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Mouse anti SARS-CoV nucleoprotein antibody recognizes nucleoprotein, also known as nucleocapsid protein and Protein N.

The nucleoprotein (N) is the most abundant viral protein in SARS-CoV-infected cells (Chang et al. 2014). It is one of the four structural proteins essential for viral assembly, alongside the spike (S), envelope (E), and membrane (M) proteins (Bartlam et al. 2005). The nucleoprotein encloses the viral genome; during assembly of the virion, nucleoprotein binds to viral RNA and forms the helical nucleocapsid (Zhu et al. 2004). It appears to be a multifunctional protein, and plays an important role in enhancing viral transcription and assembly (McBride et al. 2014). The N protein of a range of coronaviruses are abundantly expressed and are highly immunogenic, and high concentrations of anti-nucleoprotein IgG antibodies have been detected in patients with SARS. These properties have made the nucleoprotein of SARS-CoV-2 a target of interest for developing a vaccine against COVID-19 (Dutta et al. 2020).

Target Species
Viral
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Recombinant fragment aa1-49 of the SARS nucleoprotein
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1.0 ug/ml
Western Blotting 1.0 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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UniProt
P59595
GO Terms
GO:0003723 RNA binding
GO:0030529 ribonucleoprotein complex
GO:0005198 structural molecule activity
GO:0019013 viral nucleocapsid
GO:0044172 host cell endoplasmic reticulum-Golgi intermediate compartment
GO:0044177 host cell Golgi apparatus
GO:0044220 host cell perinuclear region of cytoplasm