CD45R antibody | 20.96
Mouse anti Sheep CD45R
- Product Type
- Monoclonal Antibody
- Clone
- 20.96
- Isotype
- IgG1
- Specificity
- CD45R
Antibody Quality and Performance Guarantee
| Mouse anti Sheep CD45R antibody, clone 20.96 recognizes the ovine CD45R cell surface antigen, a ~220 kDa glycoprotein, expressed by all B lymphocytes and a subset of T lymphocytes. In immunohistochemical staining the antibody labels all lymphocytes within the B cell follicles as well as scattered cells in interfollicular areas. |
- Target Species
- Sheep
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Sheep thymocytes
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the mouse NS-1 myeloma cell line
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/50 | 1/100 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 1 x 106 cells in 100μl
Source Reference
-
Mackay, C. et al. (1987) A monoclonal antibody to the p220 component of sheep LCA identifies B cells and a unique lymphocyte subset.
Cell. Immunol. 110: 46 -55.
References for CD45R antibody
-
Lee, C.S. et al. (1988) Identification of a unique lymphocyte subpopulation in the sheep uterus.
Immunology. 63: 157-64. -
Dutia, B.M. et al. (1993) Analysis of the monoclonal antibodies comprising WC6.
Vet Immunol Immunopathol. 39: 193-9. -
Leemans, I. et al. (2001) Comparative studies on surface phenotypes of Theileria lestoquardi and T. annulata schizont-infected cells.
Parasitol Res. 87: 768-77. -
Sedgmen, B.J. et al. (2002) Cellular and molecular characterisation of the ovine rectal mucosal environment.
Vet Immunol Immunopathol. 86: 215-20. -
Debes, G.F. et al. (2005) Chemokine receptor CCR7 required for T lymphocyte exit from peripheral tissues.
Nat Immunol. 6: 889-94. -
Boppana, D.K. et al. (2005) Cellular infiltration at skin lesions and draining lymph nodes of sheep infested with adult Hyalomma anatolicum anatolicum ticks.
Parasitology. 131: 657-67. -
Tanaka, Y. et al. (2008) Sustained macroscopic engraftment of cynomolgus embryonic stem cells in xenogeneic large animals after in utero transplantation.
Stem Cells Dev. 17: 367-81. -
Olivier, M. et al. (2012) Capacities of Migrating CD1b Lymph Dendritic Cells to Present Salmonella Antigens to Naive T Cells.
PLoS One. 7: e30430.
View The Latest Product References
-
Lacroux, C. et al. (2012) Prionemia and leukocyte-platelet-associated infectivity in sheep transmissible spongiform encephalopathy models.
J Virol. 86 (4): 2056-66. -
Flameng, W. et al. (2014) Coating with fibronectin and stromal cell-derived factor-1α of decellularized homografts used for right ventricular outflow tract reconstruction eliminates immune response-related degeneration.
J Thorac Cardiovasc Surg. 147 (4): 1398-404.e2.
MCA2221GA
150233
168258
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