CD45 antibody | VPM18
Mouse anti Sheep CD45
- Product Type
- Monoclonal Antibody
- Clone
- VPM18
- Isotype
- IgG1
- Specificity
- CD45
Antibody Quality and Performance Guarantee
| Mouse anti Sheep CD45, clone VPM18 recognizes the ovine homologue of human CD45 otherwise known as LCA (leucocyte common antigen), a type I transmembrane glycoprotein and member of the protein-tyrosine phosphatase (PTP) family, expressed at high levels on all nucleated cells of haematopoietic origin, and which exists in several isoforms generated by the alternative splicing. CD45 is a signal transduction protein vital for T and B cell antigen receptor-mediated activation, and is involved in many aspects of cellular processes, including growth, differentiation and oncogenic transformation. CD45 associates with many cell surface antigens, including CD2, CD3, CD4, CD26 and CD90. Mouse anti Sheep CD45, clone VPM18 recognizes all isoforms of the ovine CD45 molecule. |
- Target Species
- Sheep
- Species Cross-Reactivity
-
Target Species Cross Reactivity Human Bovine - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Sheep T lymphocytes.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS0 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/25 | 1/200 |
| Immunohistology - Frozen | |
||
| Immunoprecipitation | |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse anti Sheep CD45R | MCA2221GA | F | 0.1 mg |
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| List Price | Your Price | ||||||
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| Description | Mouse anti Sheep CD45R | ||||||
| Mouse anti Sheep CD45R:RPE | MCA2221PE | F | 100 Tests | Log in | |||
| List Price | Your Price | ||||||
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| Description | Mouse anti Sheep CD45R:RPE | ||||||
| Mouse anti Bovine CD14:FITC | MCA2678F | F | 0.1 mg |
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| List Price | Your Price | ||||||
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| Description | Mouse anti Bovine CD14:FITC | ||||||
| Mouse anti Bovine CD14 | MCA2678GA | F IF | 0.1 mg |
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| List Price | Your Price | ||||||
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| Description | Mouse anti Bovine CD14 | ||||||
References for CD45 antibody
-
Hopkins, J. & Dutia, B.M. (1990) Monoclonal antibodies to the sheep analogues of human CD45 (leucocyte common antigen), MHC class I and CD5. Differential expression after lymphocyte activation in vivo.
Vet Immunol Immunopathol. 24 (4): 331-46. -
Pastoret P-P., et. al. (1998) Immunology of cattle (in Handbook of Veterbrate Immunology)
Chapter XIII, p445 -
Darzi, S. et al. (2016) Tissue response to collagen containing polypropylene meshes in an ovine vaginal repair model.
Acta Biomater. 39: 114-23.
- Synonyms
- LCA
- RRID
- AB_1220450
MCA896GA
0313
163722
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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