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CD45R antibody | 20.96

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Staining of sheep peripheral blood lymphocytes with Mouse anti Sheep CD45R:RPE (MCA2221PE)

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Mouse anti Sheep CD45R:RPE

Product Type
Monoclonal Antibody
Clone
20.96
Isotype
IgG1
Specificity
CD45R

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Product Code Applications Pack Size List Price Your Price Qty
MCA2221PE
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 100 Tests loader
List Price Your Price
loader

Mouse anti Sheep CD45R antibody, clone 20.96 recognizes the ovine CD45R cell surface antigen, a ~220 kDa glycoprotein, expressed by all B lymphocytes and a subset of T lymphocytes. In immunohistochemical staining the antibody labels all lymphocytes within the B cell follicles as well as scattered cells in interfollicular areas.

Target Species
Sheep
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
1% bovine serum albumin
5% sucrose
Immunogen
Sheep thymocytes
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells of the mouse NS-1 myeloma cell line
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE 488nm laser 496 578
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at +4°C.
After reconstitution store at +4°C.
DO NOT FREEZE.
This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 1 x 106 cells in 100μl

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:RPE MCA928PE F 100 Tests
List Price Your Price
Description Mouse IgG1 Negative Control:RPE

Source Reference

  1. Mackay, C. et al. (1987) A monoclonal antibody to the p220 component of sheep LCA identifies B cells and a unique lymphocyte subset.
    Cell. Immunol. 110: 46 -55.

References for CD45R antibody

  1. Lee, C.S. et al. (1988) Identification of a unique lymphocyte subpopulation in the sheep uterus.
    Immunology. 63: 157-64.
  2. Dutia, B.M. et al. (1993) Analysis of the monoclonal antibodies comprising WC6.
    Vet Immunol Immunopathol. 39: 193-9.
  3. Leemans, I. et al. (2001) Comparative studies on surface phenotypes of Theileria lestoquardi and T. annulata schizont-infected cells.
    Parasitol Res. 87: 768-77.
  4. Sedgmen, B.J. et al. (2002) Cellular and molecular characterisation of the ovine rectal mucosal environment.
    Vet Immunol Immunopathol. 86: 215-20.
  5. Debes, G.F. et al. (2005) Chemokine receptor CCR7 required for T lymphocyte exit from peripheral tissues.
    Nat Immunol. 6: 889-94.
  6. Boppana, D.K. et al. (2005) Cellular infiltration at skin lesions and draining lymph nodes of sheep infested with adult Hyalomma anatolicum anatolicum ticks.
    Parasitology. 131: 657-67.
  7. Tanaka, Y. et al. (2008) Sustained macroscopic engraftment of cynomolgus embryonic stem cells in xenogeneic large animals after in utero transplantation.
    Stem Cells Dev. 17: 367-81.
  8. Olivier, M. et al. (2012) Capacities of Migrating CD1b Lymph Dendritic Cells to Present Salmonella Antigens to Naive T Cells.
    PLoS One. 7: e30430.
    9. View The Latest Product References
  9. Lacroux, C. et al. (2012) Prionemia and leukocyte-platelet-associated infectivity in sheep transmissible spongiform encephalopathy models.
    J Virol. 86 (4): 2056-66.
  10. Flameng, W. et al. (2014) Coating with fibronectin and stromal cell-derived factor-1α of decellularized homografts used for right ventricular outflow tract reconstruction eliminates immune response-related degeneration.
    J Thorac Cardiovasc Surg. 147 (4): 1398-404.e2.

Flow Cytometry

RRID
AB_609598

MCA2221PE

151292

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