CD41/CD61 antibody | CO.35E4
Mouse anti Sheep CD41/CD61
- Product Type
- Monoclonal Antibody
- Clone
- CO.35E4
- Isotype
- IgG1
- Specificity
- CD41/CD61
Antibody Quality and Performance Guarantee
| Mouse anti Sheep CD41/CD61 antibody, clone CO.35E4 recognizes ruminant CD41/CD61, also known as GpIIb/IIIa. CD41/CD61 is a calcium-complex dependent heterodimer and is expressed almost exclusively on platelets and on their precursors, the megakaryocytes. This complex is an activation-dependent receptor for RGD (Arg-Gly-Asp)-containing glycoproteins such as fibrinogen, fibronectin, von Willebrand factor, thrombospondin and vitronectin. It plays a central role in platelet aggregation and haemostasis. |
- Target Species
- Sheep
- Species Cross-Reactivity
-
Target Species Cross Reactivity Goat Cat Rabbit Dog Horse Bovine - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Sheep leucocytes.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized mice were fused the cells of the SP2-0/Ag14 mouse myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
||
| Flow Cytometry | |
1/50 | 1/200 |
| Immunoprecipitation | |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in his/her own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
References for CD41/CD61 antibody
-
Pintado, C.O. et al. (1995) Characterisation of a membrane receptor on ruminants and equine platelets and peripheral blood leukocytes similar to the human integrin receptor glycoprotein IIb/IIIa (CD41/61).
Vet Immunol Immunopathol. 44 (3-4): 359-68. -
Brodersen, R. et al. (1998) Analysis of the immunological cross reactivities of 213 well characterized monoclonal antibodies with specificities against various leucocyte surface antigens of human and 11 animal species.
Vet Immunol Immunopathol. 64 (1): 1-13. -
Lalko, C.C. et al. (2003) Equine platelet CD62P (P-selectin) expression: a phenotypic and morphologic study.
Vet Immunol Immunopathol. 91 (2): 119-34. -
Major, T.C. et al. (2010) The attenuation of platelet and monocyte activation in a rabbit model of extracorporeal circulation by a nitric oxide releasing polymer.
Biomaterials. 31: 2736-45. -
Brainard, B.M. et al. (2012) Treatment with aspirin or clopidogrel does not affect equine platelet expression of P selectin or platelet-neutrophil aggregates.
Vet Immunol Immunopathol. 149: 119-25. -
Iwaszko-Simonik, A. et al. (2015) Expression of surface platelet receptors (CD62P and CD41/61) in horses with recurrent airway obstruction (RAO).
Vet Immunol Immunopathol. 164 (1-2): 87-92. -
Finding, E.J.T. et al. (2018) Cell specific microvesicles vary with season and disease predisposition in healthy and previously laminitic ponies.
Vet Immunol Immunopathol. 202: 85-92. -
Theuerkauf, K. et al. (2022) Activated platelets and platelet-leukocyte aggregates in the equine systemic inflammatory response syndrome.
J Vet Diagn Invest. 10406387221077969.
View The Latest Product References
- Synonyms
- GPIIb-IIIa
MCA1095GA
151142
161500
168537
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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