Mouse anti Sheep CD41/CD61 antibody, clone CO.35E4 recognizes ruminant CD41/CD61, also known as GpIIb/IIIa. CD41/CD61 is a calcium-complex dependent heterodimer and is expressed almost exclusively on platelets and on their precursors, the megakaryocytes. This complex is an activation-dependent receptor for RGD (Arg-Gly-Asp)-containing glycoproteins such as fibrinogen, fibronectin, von Willebrand factor, thrombospondin and vitronectin. It plays a central role in platelet aggregation and haemostasis.
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Sheep leucocytes.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised mice were fused the cells of the SP2-0/Ag14 mouse myeloma cell line.
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of CD41/CD61 antibody
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in his/her own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
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Secondary Antibodies Available
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Product Specific References
References for CD41/CD61 antibody
Brodersen, R. et al. (1998) Analysis of the immunological cross reactivities of 213 well characterized monoclonal antibodies with specificities against various leucocyte surface antigens of human and 11 animal species.
Vet Immunol Immunopathol. 64 (1): 1-13.
Pintado, C.O. et al. (1995) Characterisation of a membrane receptor on ruminants and equine platelets and peripheral blood leukocytes similar to the human integrin receptor glycoprotein IIb/IIIa (CD41/61).
Vet Immunol Immunopathol. 44 (3-4): 359-68.
Lalko, C.C. et al. (2003) Equine platelet CD62P (P-selectin) expression: a phenotypic and morphologic study.
Vet Immunol Immunopathol. 91 (2): 119-34.
Major, T.C. et al. (2010) The attenuation of platelet and monocyte activation in a rabbit model of extracorporeal circulation by a nitric oxide releasing polymer.
Biomaterials. 31: 2736-45.
Brainard, B.M. et al. (2012) Treatment with aspirin or clopidogrel does not affect equine platelet expression of P selectin or platelet-neutrophil aggregates.
Vet Immunol Immunopathol. 149: 119-25.
Iwaszko-Simonik, A. et al. (2015) Expression of surface platelet receptors (CD62P and CD41/61) in horses with recurrent airway obstruction (RAO).
Vet Immunol Immunopathol. 164 (1-2): 87-92.
Finding, E.J.T. et al. (2018) Cell specific microvesicles vary with season and disease predisposition in healthy and previously laminitic ponies.
Vet Immunol Immunopathol. 202: 85-92.
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