CD4 antibody | 44.38
Mouse anti Sheep CD4 antibody, clone 44.38 (MCA2213GA) used for the detection of CD4 expressing cells in lymphoid tissues by immunofluorescence microscopy.
Image caption:
Distribution of ovine NKp46+ cells in lymph nodes and mucosal surfaces. Sections from ovine pre-scapular lymph node (A - cortex and B - medullary zone), abomasum (C), duodenum (D), ileum (E), colon (F), uterus (G) and lung (H). NKp46+ cells: green; B-cells (IgM+) red; CD4+ T-cells blue in panel A and B; Epithelial cells (E-cadherin+) blue in parts C-H. White arrows: individual NKp46+ cells. Red arrows: putative intra-epithelial NKp46+ cells. "Fo": follicle and "P": paracortex. "L": lymphocytic aggregate. Isotype matched controls demonstrated diffuse and dull background fluorescence in both the red and green channels (data not shown), however this could easily be distinguished by character and/or intensity from the bright cell-surface staining seen with the NKp46 and IgM-specific antibodies. Autofluorescent artifacts such as those demonstrated by the yellow arrows (e.g. in C and D) were not uncommon in the tissues examined. A scale for each of the figure parts is given. Images are taken from tissues that were representative of samples taken from a minimum of three animals as determined by preliminary single colour immuncytochemistry analysis.
From: Connelley, T. et al. NKp46 defines ovine cells that have characteristics corresponding to NK cells. Vet Res. 2011 42: 37.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Sheep CD4 antibody, clone 44.38 (MCA2213GA) used for the evaluation of CD4 expression on ovine peripheral blood mononuclear cells by flow cytometry.
Image caption:
Analysis of the phenotype of ovine NKp46+ populations. (A)Two-colour flow cytometry analysis of ovine PBMC co-stained with EC1.1 and a selection of monoclonal antibodies against CD2, CD4, CD8, CD11b, CD16, WC1, IgM and MHCII. WC1 and IgM are surface molecules expressed by subsets of γδ T-cells and B-cells respectively. (B) Perforin expression by NKp46+ PBMC. Cells expressing NKp46 were stained with anti-perforin monoclonal antibody (solid line) or an isotype control (broken line). (C) Two-colour flow cytometry analysis of mesenteric and pre-scapular lymph nodes and the spleen cell populations co-stained with EC1.1 and monoclonal antibodies against CD2 and CD8. The results shown are representative of the results obtained from a minimum of five animals.
From: Connelley, T. et al. NKp46 defines ovine cells that have characteristics corresponding to NK cells. Vet Res. 2011 42: 37.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Phycoerythrin conjugated Mouse anti Bovine CD4 antibody, clone 44.38 (MCA2213PE) used for the evaluation of CD4 expression on ovine peritoneal cells by flow cytometry.
Image caption:
Percentage of cell populations in the peritoneal fluid during the early stages of infection. The specific cell populations are shown as follows: (a) TCD4; (b) TCD8; (c) WC1+γδ; (d) CD14; (e) MHCII; (f) CD83. Cell subsets of uninfected (Neg. Ctl) and infected sheep (Group 1) are shown in columns. Mann-Whitney U-test was used to compare data from Groups 1 and 3. Values represent the mean ± standard deviation (SD). Asterisks indicate different levels of significance between groups: *P ≤ 0.05, **P ≤ 0.01
From: Pérez-Caballero R, Javier Martínez-Moreno F, Zafra R, Molina-Hernández V, Pacheco IL, Teresa Ruiz-Campillo M, Escamilla A, Pérez J, Martnez-Moreno Á, Buffoni L.
Comparative dynamics of peritoneal cell immunophenotypes in sheep during the early and late stages of the infection with Fasciola hepatica by flow cytometric analysis.
Parasit Vectors. 2018 11(1):640.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Sheep CD4 antibody, clone 44.38 (MCA2213) used to identify CD4 expressing lymphocytes from sheep peripheral blood by flow cytometry
Image caption:
Cells were collected and stained with anti-CD4/CD8 antibodies after being fixed and permeabilized. Data were acquired by flow cytometry.
Gating strategy for flow cytometry. Data are presented as means.
From: Yang J, Lv Y, Zhu Y, Li S, Tao J, Chang L, Zhu M, Zhao J, Wang Y, Wu C, Zhao W.
Baseline T-lymphocyte and cytokine indices in sheep peripheral blood.
BMC Vet Res. 2022 May 5;18(1):165.
doi: 10.1186/s12917-022-03268-7.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Sheep CD4 antibody, clone 44.38 (MCA2213) used to identify CD4 expressing lymphocytes from sheep peripheral blood by flow cytometry
Image caption:
Cells were collected and stained with anti-CD4/CD8 antibodies and intracellular cytokine antibodies after being fixed and permeabilized. Data were acquired by flow cytometry.
Gating strategy for flow cytometry. Data are presented as means.
From: Yang J, Lv Y, Zhu Y, Li S, Tao J, Chang L, Zhu M, Zhao J, Wang Y, Wu C, Zhao W.
Baseline T-lymphocyte and cytokine indices in sheep peripheral blood.
BMC Vet Res. 2022 May 5;18(1):165.
doi: 10.1186/s12917-022-03268-7.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Sheep CD4 antibody, clone 44.38 (MCA2213GA) used to label sheep peripheral blood mononuclear cells by flow cytometry.
Image caption:
Gating Strategy forFlow Cytometry:
More that 10,000 events were acquired ba a Macsquant flow cytometer for each sample and analysed with Flowlogic software using the following strategy.
The main cell population was selected using side scatter - Area (SS-A) versus FSC-A in P1 gate. Doublet cells were excluded in gate P2. Cells from P1/P2 gate were analsed for the specific staining as shown in histogram overlays showing staining with specific antibodies (in red) in comparison with their respective isotypes (in blue). Markers (M, M1, M2, M3, M4, M5) were set up according to the control isotypes to determine the percentage of positive cells. The same strategy was applied to all samples.
From: Ducournau C, Moiré N, Carpentier R, Cantin P, Herkt C, Lantier I, Betbeder D, Dimier-Poisson I.
Effective Nanoparticle-Based Nasal Vaccine Against Latent and Congenital Toxoplasmosis in Sheep.
Front Immunol. 2020 Sep 9;11:2183.
doi: 10.3389/fimmu.2020.02183.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Sheep CD4 antibody, clone 44.38 (MCA2213GA) used to label CD4 expressing sheep lymphocytes by flow cytometry.
Image caption:
CD4+ and CD8+ T cells in PBMCs. Peripheral blood samples were collected at different immunization times, and CD4+ and CD8+ T cells in PBMCs were analyzed using flow cytometry. (A) Proportion of CD4+ and CD8+ T cells in PBMCs of each group at week 8. (B, C) show the trend of the proportion of CD4+ and CD8+ T cells at different times, respectively. (D) The ratio of CD4+ to CD8+ T cells in each group of PBMCs at week 8. (E) The trend of the ratio of CD4+ to CD8+ T cells at different times. Data were obtained from 9 sheep, and results are presented as mean ± SD (ns, P> 0.05).
From: Yang J, Zhao Y, Fu Y, Lv Y, Zhu Y, Zhu M, Zhao J, Wang Y, Wu C, Zhao W.
Recombinant antigen P29 of Echinococcus granulosus induces Th1, Tc1, and Th17 cell immune responses in sheep.
Front Immunol. 2023 Dec 11;14:1243204.
doi: 10.3389/fimmu.2023.1243204.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Sheep CD4
- Product Type
- Monoclonal Antibody
- Clone
- 44.38
- Isotype
- IgG2a
- Specificity
- CD4
Antibody Quality and Performance Guarantee
| Mouse anti Sheep CD4 antibody, clone 44.38 recognizes the ovine CD4 cell surface glycoprotein, expressed by a subset of mature T lymphocytes. Mouse anti Sheep CD4 antibody, clone 44.38 immunoprecipitates a protein of ~56 kDa under reducing conditions. |
- Target Species
- Sheep
- Species Cross-Reactivity
-
Target Species Cross Reactivity Goat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Fetal thymocytes.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the mouse P3-NS1/1-Ag-4-1 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/50 | 1/100 |
| Immunofluorescence | |
||
| Immunohistology - Frozen | |
||
| Immunoprecipitation | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
References for CD4 antibody
-
Mackay, C.R. et al. (1986) Three distinct subpopulations of sheep T lymphocytes.
Eur J Immunol. 16 (1): 19-25. -
Mackay, C.R. et al. (1986) Thymocyte subpopulations during early fetal development in sheep.
J Immunol. 136 (5): 1592-9. -
Mackay, C.R. et al. (1987) A monoclonal antibody to the p220 component of sheep LCA identifies B cells and a unique lymphocyte subset.
Cell Immunol. 110 (1): 46-55. -
Debes, G.F. et al. (2005) Chemokine receptor CCR7 required for T lymphocyte exit from peripheral tissues.
Nat Immunol. 6: 889-94. -
Foulon, E. et al. (2008) Two populations of ovine bone marrow-derived dendritic cells can be generated with recombinant GM-CSF and separated on CD11b expression.
J Immunol Methods. 339: 1-10. -
Umeshappa, C.S. et al. (2010) Cell-mediated immune response and cross-protective efficacy of binary ethylenimine-inactivated bluetongue virus serotype-1 vaccine in sheep.
Vaccine. 28: 2522-31. -
Gillan, S. et al. (2010) Identification of immune parameters to differentiate disease states among sheep infected with Mycobacterium avium subsp. paratuberculosis.
Clin Vaccine Immunol. 17: 108-17. -
Breugelmans, S. et al. (2010) Immunoassay of lymphocyte subsets in ovine palatine tonsils.
Acta Histochem. 113: 416-22.
View The Latest Product References
-
Brown, M.N. et al. (2010) Chemoattractant receptors and lymphocyte egress from extralymphoid tissue: changing requirements during the course of inflammation.
J Immunol. 185: 4873-82. -
Lacroux, C. et al. (2011) Prionemia and leuco-platelet associated infectivity in sheep TSE models.
J Virol. 86: 2056-66. -
Connelley, T. et al. (2011) NKp46 defines ovine cells that have characteristics corresponding to NK cells.
Vet Res. 42: 37. -
Summers, C. et al. (2012) The distribution of immune cells in the lungs of classical and atypical ovine pulmonary adenocarcinoma.
Vet Immunol Immunopathol. 146: 1-7. -
Lybeck, K.R. et al. (2012) Intestinal Strictures, Fibrous Adhesions and High Local Interleukin-10 Levels in Goats Infected Naturally with Mycobacterium avium subsp. paratuberculosis.
J Comp Pathol. 148: 157-72. -
Silva, A.P. et al. (2015) Encapsulated Brucella ovis Lacking a Putative ATP-Binding Cassette Transporter (ΔabcBA) Protects against Wild Type Brucella ovis in Rams.
PLoS One. 10 (8): e0136865. -
Kalyanasundaram, A. et al. (2015) Comparative immunoprophylactic efficacy of Haemonchus contortus recombinant enolase (rHcENO) and Con A purified native glycoproteins in sheep.
Exp Parasitol. 154: 98-107. -
Gómez, D. et al. (2015) Effector T Cell Egress via Afferent Lymph Modulates Local Tissue Inflammation.
J Immunol. 195 (8): 3531-6. -
Goh, S. et al. (2016) Identification of Theileria lestoquardi Antigens Recognized by CD8+ T Cells.
PLoS One. 11 (9): e0162571. -
Wattegedera, S.R. et al. (2017) Enhancing the toolbox to study IL-17A in cattle and sheep.
Vet Res. 48 (1): 20. -
Greer, A.W. et al. (2018) Immune development and performance characteristics of Romney sheep selected for either resistance or resilience to gastrointestinal nematodes.
Vet Parasitol. 250: 60-7. -
Higgins, J.L. et al. (2018) Cell mediated immune response in goats after experimental challenge with the virulent Brucella melitensis strain 16M and the reduced virulence strain Rev. 1.
Vet Immunol Immunopathol. 202: 74-84. -
Pérez-caballero, R. et al. (2018) Comparative dynamics of peritoneal cell immunophenotypes in sheep during the early and late stages of the infection with Fasciola hepatica by flow cytometric analysis.
Parasit Vectors. 11 (1): 640. -
Ramos, A. et al. (2018) Melatonin enhances responsiveness to Dichelobacter nodosus. vaccine in sheep and increases peripheral blood CD4 T lymphocytes and IgG-expressing B lymphocytes.
Vet Immunol Immunopathol. 206: 1-8. -
Curina, G. et al. (2018) Evaluation of immune responses in mice and sheep inoculated with a live attenuated Brucella melitensis. REV1 vaccine produced in bioreactor.
Vet Immunol Immunopathol. 198: 44-53. -
Baliu-piqué, M. et al. (2019) Age-related distribution and dynamics of T-cells in blood and lymphoid tissues of goats.
Dev Comp Immunol. 93: 1-10. -
Wooldridge, A.L. et al. (2019) Maternal allergic asthma during pregnancy alters fetal lung and immune development in sheep: potential mechanisms for programming asthma and allergy.
J Physiol. 597 (16): 4251-62. -
Schwarz, E.R. et al. (2020) Experimental Infection of Mid-Gestation Pregnant Female and Intact Male Sheep with Zika Virus.
Viruses. 12 (3): 291. -
Zhang, H. et al. (2020) Thiamine ameliorates inflammation of the ruminal epithelium of Saanen goats suffering from subacute ruminal acidosis.
J Dairy Sci. 103 (2): 1931-43. -
Ducournau, C. et al. (2020) Effective Nanoparticle-Based Nasal Vaccine Against Latent and Congenital Toxoplasmosis in Sheep.
Front Immunol. 11: 2183. -
Nashiruddullah, N. et al. (2021) Dermal Response to Experimental Orfvirus (ORFV) Infection in Goats, Mice and Rabbit
Indian J Anim Res. 56 (8): B-4266 1003-9. -
Yang, J. et al. (2022) Baseline T-lymphocyte and cytokine indices in sheep peripheral blood.
BMC Vet Res. 18 (1): 165. -
Yang, J. et al. (2023) Recombinant antigen P29 of Echinococcus granulosus induces Th1, Tc1, and Th17 cell immune responses in sheep.
Front Immunol. 14: 1243204.
Further Reading
-
Lybeck, K. R. et al. (2009) Neutralization of interleukin-10 from CD14(+) monocytes enhances gamma interferon production in peripheral blood mononuclear cells from Mycobacterium avium subsp. paratuberculosis-infected goats.
Clin. Vaccine. Immunol. 16: 1003-11.
- UniProt
- P05542
- GO Terms
- GO:0016020 membrane
- GO:0045058 T cell selection
MCA2213GA
148434
165851
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