CD4 antibody | 44.38
Mouse anti Sheep CD4 antibody, clone 44.38 (MCA2213GA) used for the detection of CD4 expressing cells in lymphoid tissues by immunofluorescence microscopy.
Image caption:
Distribution of ovine NKp46+ cells in lymph nodes and mucosal surfaces. Sections from ovine pre-scapular lymph node (A - cortex and B - medullary zone), abomasum (C), duodenum (D), ileum (E), colon (F), uterus (G) and lung (H). NKp46+ cells: green; B-cells (IgM+) red; CD4+ T-cells blue in panel A and B; Epithelial cells (E-cadherin+) blue in parts C-H. White arrows: individual NKp46+ cells. Red arrows: putative intra-epithelial NKp46+ cells. "Fo": follicle and "P": paracortex. "L": lymphocytic aggregate. Isotype matched controls demonstrated diffuse and dull background fluorescence in both the red and green channels (data not shown), however this could easily be distinguished by character and/or intensity from the bright cell-surface staining seen with the NKp46 and IgM-specific antibodies. Autofluorescent artifacts such as those demonstrated by the yellow arrows (e.g. in C and D) were not uncommon in the tissues examined. A scale for each of the figure parts is given. Images are taken from tissues that were representative of samples taken from a minimum of three animals as determined by preliminary single colour immuncytochemistry analysis.
From: Connelley, T. et al. NKp46 defines ovine cells that have characteristics corresponding to NK cells. Vet Res. 2011 42: 37.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Sheep CD4 antibody, clone 44.38 (MCA2213GA) used for the evaluation of CD4 expression on ovine peripheral blood mononuclear cells by flow cytometry.
Image caption:
Analysis of the phenotype of ovine NKp46+ populations. (A)Two-colour flow cytometry analysis of ovine PBMC co-stained with EC1.1 and a selection of monoclonal antibodies against CD2, CD4, CD8, CD11b, CD16, WC1, IgM and MHCII. WC1 and IgM are surface molecules expressed by subsets of γδ T-cells and B-cells respectively. (B) Perforin expression by NKp46+ PBMC. Cells expressing NKp46 were stained with anti-perforin monoclonal antibody (solid line) or an isotype control (broken line). (C) Two-colour flow cytometry analysis of mesenteric and pre-scapular lymph nodes and the spleen cell populations co-stained with EC1.1 and monoclonal antibodies against CD2 and CD8. The results shown are representative of the results obtained from a minimum of five animals.
From: Connelley, T. et al. NKp46 defines ovine cells that have characteristics corresponding to NK cells. Vet Res. 2011 42: 37.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Sheep CD4:Alexa Fluor® 647
Mouse anti Sheep CD4:FITC
Mouse anti Sheep CD4
Mouse anti Sheep CD4:RPE
- Product Type
- Monoclonal Antibody
- Clone
- 44.38
- Isotype
- IgG2a
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| MCA2213A647 | F | 100 Tests/1ml | ||||
| MCA2213F | F | 0.1 mg | ||||
| MCA2213GA | C F IF IP | 0.1 mg | ||||
| MCA2213PE | F | 100 Tests |
Mouse anti Sheep CD4 antibody, clone 44.38 recognizes the ovine CD4 cell surface glycoprotein, which is expressed by a subset of mature T lymphocytes. Mouse anti Sheep CD4 antibody, clone 44.38 immunoprecipitates a protein of ~56 kDa under reducing conditions.
Product Details
- Target Species
- Sheep
- Species Cross-Reactivity
-
Target Species Cross Reactivity Goat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Alexa Fluor® 647 - liquid
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Reconstitute with 1 ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Carrier Free
- Yes
- Immunogen
- Fetal thymocytes.
- Approx. Protein Concentrations
- IgG concentration 0.05mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse P3-NS1/1-Ag-4-1 myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 12 months from date of reconstitution.
More Information
- UniProt
- P05542 Related reagents
- GO Terms
- GO:0016020 membrane
- GO:0045058 T cell selection
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Regulatory
- For research purposes only
Applications of CD4 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | 1/10 | |
| Flow Cytometry | Neat | 1/10 | |
| Flow Cytometry | 1/50 | 1/100 | |
| Immunofluorescence | |||
| Immunohistology - Frozen | |||
| Immunoprecipitation | |||
| Flow Cytometry | Neat | 1/5 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system uising appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system uising appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system uising appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system uising appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG2a Negative Control:Alexa Fluor® 647 | MCA929A647 | 100 Tests/1ml | F | ||
| Mouse IgG2a Negative Control:FITC | MCA929F | 100 Tests | F | ||
| Mouse IgG2a Negative Control:RPE | MCA929PE | 100 Tests | F |
Product Specific References
References for CD4 antibody
-
Maddox, J.F. et al. (1985) Surface antigens, SBU-T4 and SBU-T8, of sheep T lymphocyte subsets defined by monoclonal antibodies.
Immunology. 55 (4): 739-48. -
Mackay, C.R. et al. (1986) Three distinct subpopulations of sheep T lymphocytes.
Eur J Immunol. 16 (1): 19-25. -
Mackay, C.R. et al. (1987) A monoclonal antibody to the p220 component of sheep LCA identifies B cells and a unique lymphocyte subset.
Cell Immunol. 110 (1): 46-55. -
Mackay, C.R. et al. (1986) Thymocyte subpopulations during early fetal development in sheep.
J Immunol. 136 (5): 1592-9. -
Breugelmans, S. et al. (2010) Immunoassay of lymphocyte subsets in ovine palatine tonsils.
Acta Histochem. 113: 416-22. -
Connelley, T. et al. (2011) NKp46 defines ovine cells that have characteristics corresponding to NK cells.
Vet Res. 42: 37. -
Brown, M.N. et al. (2010) Chemoattractant receptors and lymphocyte egress from extralymphoid tissue: changing requirements during the course of inflammation.
J Immunol. 185: 4873-82. -
Chan, S.S. et al. (2002) Generation and characterization of ovine dendritic cells derived from peripheral blood monocytes.
Immunology. 107: 366-72. -
Foulon, E. et al. (2008) Two populations of ovine bone marrow-derived dendritic cells can be generated with recombinant GM-CSF and separated on CD11b expression.
J Immunol Methods. 339: 1-10. -
Debes, G.F. et al. (2005) Chemokine receptor CCR7 required for T lymphocyte exit from peripheral tissues.
Nat Immunol. 6: 889-94. -
Gillan, S. et al. (2010) Identification of immune parameters to differentiate disease states among sheep infected with Mycobacterium avium subsp. paratuberculosis.
Clin Vaccine Immunol. 17: 108-17. -
Lacroux, C. et al. (2011) Prionemia and leuco-platelet associated infectivity in sheep TSE models.
J Virol. 86: 2056-66. -
Summers, C. et al. (2012) The distribution of immune cells in the lungs of classical and atypical ovine pulmonary adenocarcinoma.
Vet Immunol Immunopathol. 146: 1-7. -
Lybeck, K.R. et al. (2012) Intestinal Strictures, Fibrous Adhesions and High Local Interleukin-10 Levels in Goats Infected Naturally with Mycobacterium avium subsp. paratuberculosis.
J Comp Pathol. 148: 157-72. -
Umeshappa, C.S. et al. (2010) Cell-mediated immune response and cross-protective efficacy of binary ethylenimine-inactivated bluetongue virus serotype-1 vaccine in sheep.
Vaccine. 28: 2522-31. -
Kalyanasundaram, A. et al. (2015) Comparative immunoprophylactic efficacy of Haemonchus contortus recombinant enolase (rHcENO) and Con A purified native glycoproteins in sheep.
Exp Parasitol. 154: 98-107. -
Goh S et al. (2016) Identification of Theileria lestoquardi Antigens Recognized by CD8+ T Cells.
PLoS One. 11 (9): e0162571. -
Wattegedera, S.R. et al. (2017) Enhancing the toolbox to study IL-17A in cattle and sheep.
Vet Res. 48 (1): 20. -
Gómez, D. et al. (2015) Effector T Cell Egress via Afferent Lymph Modulates Local Tissue Inflammation.
J Immunol. 195 (8): 3531-6. -
Silva, A.P. et al. (2015) Encapsulated Brucella ovis Lacking a Putative ATP-Binding Cassette Transporter (ΔabcBA) Protects against Wild Type Brucella ovis in Rams.
PLoS One. 10 (8): e0136865. -
Greer, A.W. et al. (2018) Immune development and performance characteristics of Romney sheep selected for either resistance or resilience to gastrointestinal nematodes.
Vet Parasitol. 250: 60-7.
Further Reading
-
Lybeck, K. R. et al. (2009) Neutralization of interleukin-10 from CD14(+) monocytes enhances gamma interferon production in peripheral blood mononuclear cells from Mycobacterium avium subsp. paratuberculosis-infected goats.
Clin. Vaccine. Immunol. 16: 1003-11.
