MHC Class I RT1Ac antibody | OX-27
Mouse anti Rat MHC Class I RT1Ac antibody, clone OX-27 (MCA156R) used for the assessment of chimerism in a rat transplant model by flow cytometry.
Image caption:
Peripheral blood multilineage chimerism in Group 5. Multilineage peripheral blood persisted for 12-13 weeks following vascularized hind-limb transplant, carried out by hind-limb transplant (HLT), at a low level in Group 5. At 13 weeks following vascularized hind-limb transplant, peripheral blood chimerism could hardly be detected by flow cytometry in 5 of 6 tolerant recipients in Group 5.
From: Quan Liu, Yong Wang, Atsunori Nakao, Wensheng Zhang, Vijay Gorantla, and Xin Xiao Zheng, “Heart Allograft Tolerance Induced and Maintained by Vascularized Hind-Limb Transplant in Rats,”
Clinical and Developmental Immunology, vol. 2013, Article ID 483856, 6 pages, 2013.
Mouse anti Rat MHC class I RT1Ac antibody, clone OX-27 (MCA156R) used for the evaluation of peripheral chimerism in a rat transplant model by flow cytometry.
Image caption:
Dynamic changes in peripheral and myeloid chimerism. (a) Time kinetics of chimerism in groups 5 and 6. At POD 7, they showed almost the same chimerism level (P>0.05). At POD 21, the level of chimerism in group 6 was mildly decreased, but it increased sharply in group 5 (P<0.05). At POD 42, chimerism in group 5 returned to a total level 8.7%±0.6% but it decreased to background level in group 6 (P<0.05). In addition, donor-origin CD45RA+ B cells in group 5 prevailed beginning at POD 21 and maintained thereafter. (b) Myeloid chimerism was detectable at 6.3±0.5% at POD 100 in group 5 and it never appeared in group 6 when allografts were rejected (n = 6, P<0.05).
From: Wang Y, Zheng Z, Wang Y, Liu J, Li N, et al. (2012)
Role of Donor-Specific Regulatory T Cells in Long-Term Acceptance of Rat Hind Limb Allograft.
PLoS ONE 7(8): e43825.
Mouse anti Rat MHC class I antibody, clone OX-27 (MCA156R) used for the determination of levels of chimerism in a rat transplant model by flow cytometry and immunofluorescence
Image caption:
Flow cytometry analysis for myeloid chimerism and immunocytofluorescence staining for the presence of donor-origin cells in recipients' innate femurs. (a)The proportion of donor-origin cells observed from 2 representative recipients at POD 30 were at 5.7% and 5.5%, respectively, with the background level assessed at 0.1%. (b, c) The presence of RT1n cells in the contralateral femurs of recipients in group 5 and their absence in other groups was confirmed by immunocytofluorescence (×20).
From: Wang Y, Zheng Z, Wang Y, Liu J, Li N, et al. (2012)
Role of Donor-Specific Regulatory T Cells in Long-Term Acceptance of Rat Hind Limb Allograft.
PLoS ONE 7(8): e43825.
Mouse anti Rat MHC Class I RT1Ac:FITC
Mouse anti Rat MHC Class I RT1Ac
Mouse anti Rat MHC Class I RT1Ac:RPE
- Product Type
- Monoclonal Antibody
- Clone
- OX-27
- Isotype
- IgG2a
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| MCA156F | F | 0.1 mg | ||||
| MCA156GA | C F | 0.1 mg | ||||
| MCA156R | C F | 0.25 mg | ||||
| MCA156PE | F | 100 Tests |
Mouse anti Rat MHC Class I RT1Ac antibody, clone OX-27 recognizes a polymorphic determinant (c haplotype) of rat Class I MHC Antigen (RT-1A).
Product Details
- Target Species
- Rat
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Reconstitute with 1 ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1.0% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide - Preservative Stabilisers
0.09% Sodium Azide 1.0% Bovine Serum Albumin 5% Sucrose - Carrier Free
- Yes
- Immunogen
- PHA activated rat lymphocytes.
- Approx. Protein Concentrations
- Isotype concentration 0.1 mg/ml
- Approx. Protein Concentrations
- Isotype concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.
This product should be stored undiluted.
DO NOT FREEZE. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 18 months from date of reconstitution.
More Information
- Regulatory
- For research purposes only
Applications of MHC Class I RT1Ac antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | ||
| Flow Cytometry | 1/50 | 1/100 Pack Size: 0.1 mg 1/50 Pack Size: 0.25 mg |
|
| Immunohistology - Frozen | |||
| Flow Cytometry | Neat |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG2a Negative Control:FITC | MCA1210F | 0.1 mg | F | ||
| Mouse IgG2a Negative Control | MCA1210 | 100 Tests | F | ||
| Mouse IgG2a Negative Control:RPE | MCA1210PE | 100 Tests | F |
Product Specific References
References for MHC Class I RT1Ac antibody
-
Jefferies, W.A. et al. (1985) Analysis of lymphopoietic stem cells with a monoclonal antibody to the rat transferrin receptor.
Immunology. 54 (2): 333-41. -
Barclay, A.N. (1981) The localization of populations of lymphocytes defined by monoclonal antibodies in rat lymphoid tissues.
Immunology 42: 593-600. -
Huang, W.C. et al. (2010) Vascularized bone grafts within composite tissue allotransplants can autocreate tolerance through mixed chimerism with partial myeloablative conditioning: an experimental study in rats.
Plast Reconstr Surg. 125 (4): 1095-103. -
Hikita, N. et al. (1997) Use of topical FK506 in a corneal graft rejection model in Lewis rats.
Invest Ophthalmol Vis Sci. 38 (5): 901-9. -
Sharland, A. et al. (1999) Evidence that apoptosis of activated T cells occurs in spontaneous tolerance of liver allografts and is blocked by manipulations which break tolerance.
Transplantation. 68:1736-45. -
Zhu H et al. (2015) Rat model of heterotopic toe allotransplantation.
J Surg Res. pii: S0022-4804(15)00283-8. -
Liu, Q. et al. (2013) Heart allograft tolerance induced and maintained by vascularized hind-limb transplant in rats.
Clin Dev Immunol. 2013: 483856. -
Wang Y et al. (2012) Role of donor-specific regulatory T cells in long-term acceptance of rat hind limb allograft.
PLoS One. 7 (8): e43825. -
von Websky, M.W. et al. (2016) Recombinant HLA-G as Tolerogenic Immunomodulant in Experimental Small Bowel Transplantation.
PLoS One. 11 (7): e0158907. -
Gu, C. et al. (2016) Triptolide Reduces the Required Dose of Tacrolimus by Attenuating Inflammation, Enhancing Immunosuppression, and Increasing Donor Chimerism in a Heterotopic Hindlimb Transplantation Model.
Plast Reconstr Surg. 138 (6): 1243-1253.
