MHC Class I RT1Ac antibody | OX-27

Mouse anti Rat MHC Class I RT1Ac

Product Type
Monoclonal Antibody
Clone
OX-27
Isotype
IgG2a
Specificity
MHC Class I RT1Ac

Product Code Applications Pack Size List Price Your Price Qty
MCA156GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F 0.1 mg loader
List Price Your Price
loader
MCA156R
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F 0.25 mg loader
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loader
Search for Batch Specific Datasheets

Mouse anti Rat MHC Class I RT1Ac antibody, clone OX-27 recognizes a polymorphic determinant (c haplotype) of rat Class I MHC Antigen (RT-1A).

Target Species
Rat
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
PHA activated rat lymphocytes.
Approx. Protein Concentrations
Isotype concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 1/100 Pack Size: 0.1 mg
1/50 Pack Size: 0.25 mg
Immunohistology - Frozen
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

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Description Product Code Applications Pack Size List Price Your Price Quantity
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Description Mouse IgG2a Negative Control

References for MHC Class I RT1Ac antibody

  1. Jefferies, W.A. et al. (1985) Analysis of lymphopoietic stem cells with a monoclonal antibody to the rat transferrin receptor.
    Immunology. 54 (2): 333-41.
  2. Barclay, A.N. (1981) The localization of populations of lymphocytes defined by monoclonal antibodies in rat lymphoid tissues.
    Immunology 42: 593-600.
  3. Huang, W.C. et al. (2010) Vascularized bone grafts within composite tissue allotransplants can autocreate tolerance through mixed chimerism with partial myeloablative conditioning: an experimental study in rats.
    Plast Reconstr Surg. 125 (4): 1095-103.
  4. Hikita, N. et al. (1997) Use of topical FK506 in a corneal graft rejection model in Lewis rats.
    Invest Ophthalmol Vis Sci. 38 (5): 901-9.
  5. Sharland, A. et al. (1999) Evidence that apoptosis of activated T cells occurs in spontaneous tolerance of liver allografts and is blocked by manipulations which break tolerance.
    Transplantation. 68:1736-45.
  6. Zhu H et al. (2015) Rat model of heterotopic toe allotransplantation.
    J Surg Res. pii: S0022-4804(15)00283-8.
  7. Liu, Q. et al. (2013) Heart allograft tolerance induced and maintained by vascularized hind-limb transplant in rats.
    Clin Dev Immunol. 2013: 483856.
  8. Wang Y et al. (2012) Role of donor-specific regulatory T cells in long-term acceptance of rat hind limb allograft.
    PLoS One. 7 (8): e43825.
  9. View The Latest Product References
  10. von Websky, M.W. et al. (2016) Recombinant HLA-G as Tolerogenic Immunomodulant in Experimental Small Bowel Transplantation.
    PLoS One. 11 (7): e0158907.
  11. Gu, C. et al. (2016) Triptolide Reduces the Required Dose of Tacrolimus by Attenuating Inflammation, Enhancing Immunosuppression, and Increasing Donor Chimerism in a Heterotopic Hindlimb Transplantation Model.
    Plast Reconstr Surg. 138 (6): 1243-1253.
  12. Schweizer, R. et al. (2020) Adipose-derived stromal cell therapy combined with a short course nonmyeloablative conditioning promotes long-term graft tolerance in vascularized composite allotransplantation.
    Am J Transplant. 20 (5): 1272-84.

RRID
AB_323877
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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