MHC Class I RT1Ac antibody | OX-27
Mouse anti Rat MHC Class I RT1Ac antibody, clone OX-27 (MCA156R) used for the assessment of chimerism in a rat transplant model by flow cytometry.
Image caption:
Peripheral blood multilineage chimerism in Group 5. Multilineage peripheral blood persisted for 12-13 weeks following vascularized hind-limb transplant, carried out by hind-limb transplant (HLT), at a low level in Group 5. At 13 weeks following vascularized hind-limb transplant, peripheral blood chimerism could hardly be detected by flow cytometry in 5 of 6 tolerant recipients in Group 5.
From: Quan Liu, Yong Wang, Atsunori Nakao, Wensheng Zhang, Vijay Gorantla, and Xin Xiao Zheng, “Heart Allograft Tolerance Induced and Maintained by Vascularized Hind-Limb Transplant in Rats,”
Clinical and Developmental Immunology, vol. 2013, Article ID 483856, 6 pages, 2013.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat MHC class I antibody, clone OX-27 (MCA156R) used for the determination of levels of chimerism in a rat transplant model by flow cytometry and immunofluorescence
Image caption:
Flow cytometry analysis for myeloid chimerism and immunocytofluorescence staining for the presence of donor-origin cells in recipients' innate femurs. (a)The proportion of donor-origin cells observed from 2 representative recipients at POD 30 were at 5.7% and 5.5%, respectively, with the background level assessed at 0.1%. (b, c) The presence of RT1n cells in the contralateral femurs of recipients in group 5 and their absence in other groups was confirmed by immunocytofluorescence (×20).
From: Wang Y, Zheng Z, Wang Y, Liu J, Li N, et al. (2012)
Role of Donor-Specific Regulatory T Cells in Long-Term Acceptance of Rat Hind Limb Allograft.
PLoS ONE 7(8): e43825.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat MHC class I RT1Ac antibody, clone OX-27 (MCA156R) used for the evaluation of peripheral chimerism in a rat transplant model by flow cytometry.
Image caption:
Dynamic changes in peripheral and myeloid chimerism. (a) Time kinetics of chimerism in groups 5 and 6. At POD 7, they showed almost the same chimerism level (P>0.05). At POD 21, the level of chimerism in group 6 was mildly decreased, but it increased sharply in group 5 (P<0.05). At POD 42, chimerism in group 5 returned to a total level 8.7%±0.6% but it decreased to background level in group 6 (P<0.05). In addition, donor-origin CD45RA+ B cells in group 5 prevailed beginning at POD 21 and maintained thereafter. (b) Myeloid chimerism was detectable at 6.3±0.5% at POD 100 in group 5 and it never appeared in group 6 when allografts were rejected (n = 6, P<0.05).
From: Wang Y, Zheng Z, Wang Y, Liu J, Li N, et al. (2012)
Role of Donor-Specific Regulatory T Cells in Long-Term Acceptance of Rat Hind Limb Allograft.
PLoS ONE 7(8): e43825.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat MHC Class I RT1Ac:FITC
Mouse anti Rat MHC Class I RT1Ac
Mouse anti Rat MHC Class I RT1Ac:RPE
- Product Type
- Monoclonal Antibody
- Clone
- OX-27
- Isotype
- IgG2a
| Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|
| MCA156F | F | 0.1 mg |
|
|
| MCA156GA | C F | 0.1 mg |
|
|
| MCA156R | C F | 0.25 mg |
|
|
| MCA156PE | F | 100 Tests |
|
Mouse anti Rat MHC Class I RT1Ac antibody, clone OX-27 recognizes a polymorphic determinant (c haplotype) of rat Class I MHC Antigen (RT-1A).
Product Details
- Target Species
- Rat
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Reconstitute with 1 ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1.0% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide - Preservative Stabilisers
0.09% Sodium Azide 1.0% Bovine Serum Albumin 5% Sucrose - Carrier Free
- Yes
- Immunogen
- PHA activated rat lymphocytes.
- Approx. Protein Concentrations
- Isotype concentration 0.1 mg/ml
- Approx. Protein Concentrations
- Isotype concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Storage
- Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.
This product should be stored undiluted.
DO NOT FREEZE. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of MHC Class I RT1Ac antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | ||
| Flow Cytometry | 1/50 | 1/100 Pack Size: 0.1 mg 1/50 Pack Size: 0.25 mg |
|
| Immunohistology - Frozen | |||
| Flow Cytometry | Neat |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG2a Negative Control:FITC | MCA1210F | F | 0.1 mg |
|
|
| Mouse IgG2a Negative Control | MCA1210 | F | 100 Tests |
|
|
| Mouse IgG2a Negative Control:RPE | MCA1210PE | F | 100 Tests |
|
Product Specific References
References for MHC Class I RT1Ac antibody
-
Jefferies, W.A. et al. (1985) Analysis of lymphopoietic stem cells with a monoclonal antibody to the rat transferrin receptor.
Immunology. 54 (2): 333-41. -
Barclay, A.N. (1981) The localization of populations of lymphocytes defined by monoclonal antibodies in rat lymphoid tissues.
Immunology 42: 593-600. -
Huang, W.C. et al. (2010) Vascularized bone grafts within composite tissue allotransplants can autocreate tolerance through mixed chimerism with partial myeloablative conditioning: an experimental study in rats.
Plast Reconstr Surg. 125 (4): 1095-103. -
Hikita, N. et al. (1997) Use of topical FK506 in a corneal graft rejection model in Lewis rats.
Invest Ophthalmol Vis Sci. 38 (5): 901-9. -
Sharland, A. et al. (1999) Evidence that apoptosis of activated T cells occurs in spontaneous tolerance of liver allografts and is blocked by manipulations which break tolerance.
Transplantation. 68:1736-45. -
Zhu H et al. (2015) Rat model of heterotopic toe allotransplantation.
J Surg Res. pii: S0022-4804(15)00283-8. -
Liu, Q. et al. (2013) Heart allograft tolerance induced and maintained by vascularized hind-limb transplant in rats.
Clin Dev Immunol. 2013: 483856. -
Wang Y et al. (2012) Role of donor-specific regulatory T cells in long-term acceptance of rat hind limb allograft.
PLoS One. 7 (8): e43825. -
von Websky, M.W. et al. (2016) Recombinant HLA-G as Tolerogenic Immunomodulant in Experimental Small Bowel Transplantation.
PLoS One. 11 (7): e0158907. -
Gu, C. et al. (2016) Triptolide Reduces the Required Dose of Tacrolimus by Attenuating Inflammation, Enhancing Immunosuppression, and Increasing Donor Chimerism in a Heterotopic Hindlimb Transplantation Model.
Plast Reconstr Surg. 138 (6): 1243-1253. -
Schweizer, R. et al. (2020) Adipose-derived stromal cell therapy combined with a short course nonmyeloablative conditioning promotes long-term graft tolerance in vascularized composite allotransplantation.
Am J Transplant. 20 (5): 1272-84.
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