IgM antibody | MARM-4

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Mouse anti Rat IgM Heavy Chain:Biotin

Mouse anti Rat IgM Heavy Chain:FITC

Mouse anti Rat IgM Heavy Chain:HRP

Mouse anti Rat IgM Heavy Chain

Product Type: Monoclonal Antibody
Clone: MARM-4
Isotype: IgG1

Product Code	Applications	Pack Size
MCA189B	E	0.5 mg
MCA189F	F	0.5 mg
MCA189P	E	0.5 mg
MCA189	E	0.25 mg

Mouse anti Rat IgM Heavy Chain antibody, clone MARM-4 recognizes the mu heavy chain of rat immunoglobulin and does not cross-react with other immunoglobulin classes.

Product Details

Target Species

Rat

Product Form

Purified IgG conjugated to Biotin - liquid

Product Form

Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid

Product Form

Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid

Product Form

Purified IgG - liquid

Preparation

Purified IgG prepared by affinity chromatography from tissue culture supernatant

Preparation

Purified IgG prepared by affinity chromatography from tissue culture supernatant

Preparation

Purified IgG prepared by affinity chromatography. from tissue culture supernatant

Preparation

Purified IgG prepared by affinity chromatography from tissue culture supernatant

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09%	Sodium Azide
50%	Glycerol

Preservative Stabilisers

0.1%	Sodium Azide
50%	Glycerol

Preservative Stabilisers

0.01%	Thiomersal
50%	Glycerol

Preservative Stabilisers

0.1%	Sodium Azide

Immunogen

IR202 rat IgM myeloma protein

Approx. Protein Concentrations

IgG concentration 1.0 mg/ml

Approx. Protein Concentrations

IgG concentration 1.0 mg/ml

Approx. Protein Concentrations

IgG concentration 1.0 mg/ml

Approx. Protein Concentrations

IgG concentration 1.0 mg/ml

Fusion Partners

Spleen cells from immunized BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line.

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.

More Information

Regulatory: For research purposes only

Applications of IgM antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA			125ng/ml as detecting antibody
Flow Cytometry			10ug/ml
ELISA			0.5ug/ml
ELISA			5ug/ml as coating antibody

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 50ul of the suggested working dilution to label 10⁶ cells in 100ul.
ELISA: This antibody may be used as a coating antibody in a sandwich ELISA in combination with clone MARK-1/MARL-15 (Product code MCA1296P) as detection reagent and purified rat IgM (Product code PRP08).

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Human anti Mouse IgG1:HRP	HCA036P	0.1 mg	E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Negative Isotype Controls Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Mouse IgG1 Negative Control:FITC	MCA1209F	0.1 mg	F

Useful Reagents Available

Description	Product Code	Pack Size	Applications
AbGUARD® HRP Stabilizer Plus	BUF052A	100 ml	C E P WB
AbGUARD® HRP Stabilizer Plus	BUF052B	500 ml	C E P WB
AbGUARD® HRP Stabilizer Plus	BUF052C	1000 ml	C E P WB
TMB Signal+	BUF054A	100 ml	E
TMB Core	BUF056A	100 ml	E
TMB Core+	BUF062A	100 ml	E

Product Specific References

References for IgM antibody

Bazin, H. et al. (1974) Three classes and four (sub)classes of rat immunoglobulins: IgM, IgA, IgE and IgG1, IgG2a, IgG2b, IgG2c.
Eur J Immunol. 4 (1): 44-8.
Bazin, H. et al. (1978) Transplantable IgD immunoglobulin-secreting tumors in rat.
J Immunol. 121 (5): 2077-82.
Khalifeh, M.S. et al. (2010) Investigation ofthe role of tumour necrosis factor-{alpha}, interleukin-1{beta}, interleukin-10, nitric oxide and rheumatoid factor-immunoglobulin M in a rat model of arthritis.
Lab Anim. 44: 143-9.
Bézie, S. et al. (2015) Compensatory Regulatory Networks between CD8 T, B, and Myeloid Cells in Organ Transplantation Tolerance.
J Immunol. 195 (12): 5805-15.

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