IgG2b antibody | MARG2b-8
Mouse anti Rat IgG2b Heavy Chain antibody, clone MARG2b-8 (MCA195) used for the evaluation of antibody production in rats by flow cytometry.
Image caption:
Alloantibody production was suppressed after AAV-FGL2 treatment and adoptive transfer of splenocytes. Sera were collected from naive rats, or at the moment of rejection from rats treated with AAV-control or AAV-FGL2 (rejecting at < 30 days or > 120 days after transplantation) or receiving adoptive transfers (> 120 days after transplantation). Levels of donor-specific IgG1, IgG2a, and IgG2b antibodies were evaluated by cytofluorimetry and normalized to serum from naive rats (MFI / MFI syngeneic). Two way Anova, Bonferroni post test p value * <0.5; ** <0.01; ***<0.001.
From: Bézie S, Picarda E, Tesson L, Renaudin K, Durand J, Ménoret S, et al. (2015)
Fibrinogen-Like Protein 2/Fibroleukin Induces Long-Term Allograft Survival in a Rat Model through Regulatory B Cells.
PLoS ONE 10(3): e0119686.
This is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Rat IgG2b Heavy Chain antibody, clone MARG2b-8 recognizes the rat gamma 2b immunoglobulin heavy chain, and does not cross-react with other rat immunoglobulin classes or subclasses.
The avidity of Mouse anti Rat IgG2b Heavy Chain antibody, clone MARG2b-8 for rat IgG2b = 4.7 x 109 M-1.
The avidity of Mouse anti Rat IgG2b Heavy Chain antibody, clone MARG2b-8 for rat IgG2b = 4.7 x 109 M-1.
Product Details
- Target Species
- Rat
- Product Form
- Purified IgG conjugated to Biotin - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.1% Sodium Azide 50% Glycerol - Preservative Stabilisers
- 0.1% Sodium Azide (NaN3)
- Immunogen
- IR863 rat IgG2b myeloma protein.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the mouse Sp2/0 myeloma cell line.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P20761
- GO Terms
- GO:0003823 antigen binding
- Regulatory
- For research purposes only
Applications of IgG2b antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1000ng/ml | ||
| ELISA | 5ug/ml (as a coating antibody) |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Product Specific References
References for IgG2b antibody
-
Sato, K. et al. (2001) Carbon monoxide generated by heme oxygenase-1 suppresses the rejection of mouse-to-rat cardiac transplants.
J Immunol. 166 (6): 4185-94. -
Bézie, S. et al. (2015) Fibrinogen-like protein 2/fibroleukin induces long-term allograft survival in a rat model through regulatory B cells.
PLoS One. 10 (3): e0119686. -
Bézie, S. et al. (2015) Compensatory Regulatory Networks between CD8 T, B, and Myeloid Cells in Organ Transplantation Tolerance.
J Immunol. 195 (12): 5805-15.
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