IgG2b antibody | MARG2b-8
Mouse anti Rat IgG2b Heavy Chain antibody, clone MARG2b-8 (MCA195) used for the evaluation of antibody production in rats by flow cytometry.
Image caption:
Alloantibody production was suppressed after AAV-FGL2 treatment and adoptive transfer of splenocytes. Sera were collected from naive rats, or at the moment of rejection from rats treated with AAV-control or AAV-FGL2 (rejecting at < 30 days or > 120 days after transplantation) or receiving adoptive transfers (> 120 days after transplantation). Levels of donor-specific IgG1, IgG2a, and IgG2b antibodies were evaluated by cytofluorimetry and normalized to serum from naive rats (MFI / MFI syngeneic). Two way Anova, Bonferroni post test p value * <0.5; ** <0.01; ***<0.001.
From: Bézie S, Picarda E, Tesson L, Renaudin K, Durand J, Ménoret S, et al. (2015)
Fibrinogen-Like Protein 2/Fibroleukin Induces Long-Term Allograft Survival in a Rat Model through Regulatory B Cells.
PLoS ONE 10(3): e0119686.
This is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Rat IgG2b Heavy Chain antibody, clone MARG2b-8 recognizes the rat gamma 2b immunoglobulin heavy chain, and does not cross-react with other rat immunoglobulin classes or subclasses.
The avidity of Mouse anti Rat IgG2b Heavy Chain antibody, clone MARG2b-8 for rat IgG2b = 4.7 x 109 M-1.
The avidity of Mouse anti Rat IgG2b Heavy Chain antibody, clone MARG2b-8 for rat IgG2b = 4.7 x 109 M-1.
Product Details
- Target Species
- Rat
- Product Form
- Purified IgG conjugated to Biotin - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.1% Sodium Azide 50% Glycerol - Preservative Stabilisers
- 0.1% Sodium Azide (NaN3)
- Immunogen
- IR863 rat IgG2b myeloma protein.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the mouse Sp2/0 myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P20761
- GO Terms
- GO:0003823 antigen binding
- Regulatory
- For research purposes only
Applications of IgG2b antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1000ng/ml | ||
| ELISA | 5ug/ml (as a coating antibody) |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available
Product Specific References
References for IgG2b antibody
-
Sato, K. et al. (2001) Carbon monoxide generated by heme oxygenase-1 suppresses the rejection of mouse-to-rat cardiac transplants.
J Immunol. 166 (6): 4185-94. -
Bézie, S. et al. (2015) Fibrinogen-like protein 2/fibroleukin induces long-term allograft survival in a rat model through regulatory B cells.
PLoS One. 10 (3): e0119686. -
Bézie, S. et al. (2015) Compensatory Regulatory Networks between CD8 T, B, and Myeloid Cells in Organ Transplantation Tolerance.
J Immunol. 195 (12): 5805-15.
