IgG2b antibody | MARG2b-3
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Mouse anti Rat IgG2b Heavy Chain
- Product Type
- Monoclonal Antibody
- Clone
- MARG2b-3
- Isotype
- IgG1
- Specificity
- IgG2b
- Region
- HEAVY CHAIN
Antibody Quality and Performance Guarantee
| Mouse anti Rat IgG2b Heavy Chain antibody, clone MARG2b-3 recognizes the gamma-2b heavy chain of rat immunoglobulin. |
- Target Species
- Rat
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Immunogen
- Rat IgG2b.
- Approx. Protein Concentrations
- IgG concentration 1 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
2.5ug/ml as coating antibody |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- ELISA
- Clone MARG2b-3 may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with our HRP-conjugated MARK-1/MARL-15 clone cocktail (MCA1296P) as the detection reagent and purified rat IgG2b (PRP05) as the standard.
References for IgG2b antibody
-
Le Roy, D. et al. (1999) Monoclonal antibodies to murine lipopolysaccharide (LPS)-binding protein (LBP) protect mice from lethal endotoxemia by blocking either the binding of LPS to LBP or the presentation of LPS/LBP complexes to CD14.
J Immunol. 162 (12): 7454-60. -
Ueta, H. et al. (2018) Single blood transfusion induces the production of donor-specific alloantibodies and regulatory T cells mainly in the spleen.
Int Immunol. 30 (2): 53-67.
- RRID
- AB_321837
- UniProt
- P20761
- GO Terms
- GO:0003823 antigen binding
View more products with IgG specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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