IgE antibody | MARE-1

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Anti Rat IgE Antibody, clone MARE-1 gallery image 1 — **Published customer image:**
Mouse anti Rat IgE antibody, clone MARE-1 (**MCA193**) used to stimulate mast cells *in vitro* as a positive control prior to ELISA measurement of CCL3 production.
**Image caption:**
Effect of LL-37 on CCL3 production. For CCL3 measurement, mast cells were incubated with different concentrations of LL-37, anti-IgE at 5 μg/mL (positive control), or medium alone (NS) for 2 h. Results are the mean ± SD of four independent experiments (N=8). Comparisons between groups were carried out by using Student's t-test for small groups. Differences were considered significant at P < 0.05 and are labeled with an asterisk (*) on each graph.

From: Agier J, Brzezińska-Błaszczyk E, żelechowska P, Wiktorska M, Pietrzak J, Różalska S.
Cathelicidin LL-37 Affects Surface and Intracellular Toll-Like Receptor Expression in Tissue Mast Cells.
J Immunol Res. 2018 Feb 19;2018:7357162.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Mouse anti Rat IgE:Biotin

Mouse anti Rat IgE:FITC

Mouse anti Rat IgE:HRP

Mouse anti Rat IgE

Product Type: Monoclonal Antibody
Clone: MARE-1
Isotype: IgG1

Product Code	Applications	Pack Size
MCA193B	E WB	0.5 mg
MCA193F	F	0.5 mg
MCA193P	E WB	0.5 mg
MCA193	E WB	0.25 mg

Mouse anti Rat IgE antibody, clone MARE-1 recognizes rat epsilon heavy chain of immunoglobulin IgE and does not cross-react with other classes of rat immunoglobulin.

Mouse anti Rat IgE antibody, clone MARE-1 binds to rat IgE with an avidity of 4x10⁹M^-1

Product Details

Target Species

Rat

Product Form

Purified IgG conjugated to Biotin - liquid

Product Form

Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid

Product Form

Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid

Product Form

Purified IgG - liquid

Preparation

Purified IgG prepared by affinity chromatography on IR2 rat IgE myeloma from tissue culture supernatant

Preparation

Purified IgG prepared by affinity chromatography from tissue culture supernatant

Preparation

Purified IgG prepared by affinity chromatography from tissue culture supernatant

Preparation

Purified IgG prepared by immunoaffinity on Protein G from tissue culture supernatant

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.1%	Sodium Azide
50%	Glycerol

Preservative Stabilisers

0.1%	Sodium Azide
50%	Glycerol

Preservative Stabilisers

0.01%	Thiomersal
50%	Glycerol

Preservative Stabilisers

0.1%	Sodium Azide

Immunogen

Rat IR162, IR1016, IR2 and IR410 IgE myeloma proteins.

Approx. Protein Concentrations

IgG concentration 1 mg/ml

Approx. Protein Concentrations

IgG concentration 1 mg/ml

Approx. Protein Concentrations

IgG concentration 1.0 mg/ml

Approx. Protein Concentrations

IgG concentration 1.0 mg/ml

Fusion Partners

Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line.

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee: 18 months from date of despatch.
Guarantee: 18 months from date of despatch.
Guarantee: 18 months from date of despatch.
Guarantee: 18 months from date of despatch.

More Information

UniProt: P01855 Related reagents
Entrez Gene: Ighe Related reagents
GO Terms: GO:0003823 antigen binding
Regulatory: For research purposes only

Applications of IgE antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA			500ng/ml as detecting antibody
Western Blotting
Flow Cytometry			5ug/ml
ELISA			0.5ug/ml
Western Blotting
ELISA			5ug/ml as coating antibody
Western Blotting

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 50ul of the suggested working dilution to label 2 x 10⁷ cells in 100ul.
ELISA: This antibody may be used as a coating antibody in a sandwich ELISA in combination with clone MARK-1/MARL-15 (Product code MCA1296P) as detection reagent and purified rat IgE (Product code PRP07A).

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Negative Isotype Controls Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Mouse IgG1 Negative Control:FITC	MCA1209F	0.1 mg	F

Useful Reagents Available

Description	Product Code	Pack Size	Applications
AbGUARD® HRP Stabilizer Plus	BUF052A	100 ml	C E P WB
AbGUARD® HRP Stabilizer Plus	BUF052B	500 ml	C E P WB
AbGUARD® HRP Stabilizer Plus	BUF052C	1000 ml	C E P WB
TMB Signal+	BUF054A	100 ml	E
TMB Core	BUF056A	100 ml	E
TMB Core+	BUF062A	100 ml	E

Application Based External Images

ELISA

Product Specific References

References for IgE antibody

Negrão-Corrêa, D. et al. (1996) Intestinal transport and catabolism of IgE: a major blood-independent pathway of IgE dissemination during a Trichinella spiralis infection of rats.
J Immunol. 157 (9): 4037-44.
Bazin, H. et al. (1984) Rat monoclonal antibodies. I. Rapid purification from in vitro culture supernatants.
J Immunol Methods. 66 (2): 261-9.
Bazin, H. et al. (1974) Transplantable immunoglobulin-secreting tumours in rats. IV. Sixty-three IgE-secreting immunocytoma tumours.
Immunology. 26 (4): 713-23.
Bazin, H. et al. (1978) Transplantable IgD immunoglobulin-secreting tumors in rat.
J Immunol. 121 (5): 2077-82.
Bazin, H. et al. (1984) Rat monoclonal antibodies. II. A rapid and efficient method of purification from ascitic fluid or serum.
J Immunol Methods. 71 (1): 9-16.
Cho, J.K. & Cho, S.W. (2000) Shared epitope for monoclonal IR162 between Anisakis simplex larvae and Clonorchis sinensis and cross-reactivity between antigens.
J Parasitol. 86 (5): 1145-9.
Silveira, M.R. et al. (2002) Infection with Strongyloides venezuelensis induces transient airway eosinophilic inflammation, an increase in immunoglobulin E, and hyperresponsiveness in rats.
Infect Immun. 70: 6263-72.
Korinek, M. et al. (2016) Anti-allergic potential of Typhonium blumei: inhibition of degranulation via suppression of PI3K/PLCγ2 phosphorylation and calcium influx.
Phytomedicine. 23 (14): 1706-15.
Bąbolewska, E. & Brzezińska-błaszczyk, E. (2015) Human-derived cathelicidin LL-37 directly activates mast cells to proinflammatory mediator synthesis and migratory response.
Cell Immunol. 293 (2): 67-73.
Agier, J. et al. (2018) Cathelicidin LL-37 Affects Surface and Intracellular Toll-Like Receptor Expression in Tissue Mast Cells.
J Immunol Res. 2018: 7357162.

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