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IgE antibody | MARE-1

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Anti Rat IgE Antibody, clone MARE-1 thumbnail image 1 Anti Rat IgE Antibody, clone MARE-1 thumbnail image 2
Anti Rat IgE Antibody, clone MARE-1 gallery image 1
Published customer image:
Mouse anti Rat IgE antibody, clone MARE-1 (MCA193) used to stimulate mast cells in vitro as a positive control prior to ELISA measurement of CCL3 production.
Image caption:
Effect of LL-37 on CCL3 production. For CCL3 measurement, mast cells were incubated with different concentrations of LL-37, anti-IgE at 5 μg/mL (positive control), or medium alone (NS) for 2 h. Results are the mean ± SD of four independent experiments (N=8). Comparisons between groups were carried out by using Student's t-test for small groups. Differences were considered significant at P < 0.05 and are labeled with an asterisk (*) on each graph.

From: Agier J, Brzezińska-Błaszczyk E, żelechowska P, Wiktorska M, Pietrzak J, Różalska S.
Cathelicidin LL-37 Affects Surface and Intracellular Toll-Like Receptor Expression in Tissue Mast Cells.
J Immunol Res. 2018 Feb 19;2018:7357162.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Rat IgE Antibody, clone MARE-1 gallery image 2
Published customer image:
Mouse anti Rat IgE antibody, clone MARE1 (MCA193) used as a challenge in a cytokine release assay for the determination of multiple cytokine production values in vitro.
Image caption:
FcεRI-mediated release of de novo synthesized mediators upon rPMC stimulation with TLR3 ligand. rPMCs were treated with medium alone, poly(I:C) at 10 μg/mL, anti-IgE at 5 μg/mL, or both poly (I:C) and anti-IgE for 2 (a) or 12 h (b–f). CysLT (a), IFN-α (b), IFN-β (c), TNF (d), CCL3 (e), and CXCL8 (f) were measured in cell supernatants using ELISA. Bars for the poly(I:C) at 10 μg/mL and medium alone demonstrate the same data set as in Figure 3. Results are presented as the mean ± SD of 4 independent experiments, and each experiment was done in duplicate (n = 8). ∗p <0.05, ∗∗p <0.01, and ∗∗∗p <0.001; n.s.: not significant.

From: Witczak P, Brzezińska-Błaszczyk E, Agier J.
The Response of Tissue Mast Cells to TLR3 Ligand Poly(I:C) Treatment.
J Immunol Res. 2020 Feb 24;2020:2140694.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

Mouse anti Rat IgE:Biotin

Mouse anti Rat IgE:FITC

Mouse anti Rat IgE:HRP

Mouse anti Rat IgE

Product Type
Monoclonal Antibody
Clone
MARE-1
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA193B
Datasheet
E WB
SDS
0.5 mg loader

MCA193F
Datasheet
F
SDS
0.5 mg loader

MCA193P
Datasheet
E WB
SDS
0.5 mg loader

MCA193
Datasheet
E WB
SDS
0.25 mg loader

Mouse anti Rat IgE antibody, clone MARE-1 recognizes rat epsilon heavy chain of immunoglobulin IgE and does not cross-react with other classes of rat immunoglobulin.

Mouse anti Rat IgE antibody, clone MARE-1 binds to rat IgE with an avidity of 4x109M-1

Product Details

Target Species
Rat
Product Form
Purified IgG conjugated to Biotin - liquid
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on IR2 rat IgE myeloma from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography from tissue culture supernatant
Preparation
Purified IgG prepared by immunoaffinity on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1%Sodium Azide
50%Glycerol
Preservative Stabilisers
0.1%Sodium Azide
50%Glycerol
Preservative Stabilisers
0.01%Thiomersal
50%Glycerol
Preservative Stabilisers
0.1%Sodium Azide
Immunogen
Rat IR162, IR1016, IR2 and IR410 IgE myeloma proteins.
Approx. Protein Concentrations
IgG concentration 1 mg/ml
Approx. Protein Concentrations
IgG concentration 1 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line.

Storage Information

Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

UniProt
P01855
Entrez Gene
Ighe
GO Terms
GO:0003823 antigen binding
Regulatory
For research purposes only

Applications of IgE antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 500ng/ml as detecting antibody
Western Blotting
Flow Cytometry 5ug/ml
ELISA 0.5ug/ml
Western Blotting
ELISA 5ug/ml as coating antibody
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 50ul of the suggested working dilution to label 2 x 107 cells in 100ul.
ELISA
This antibody may be used as a coating antibody in a sandwich ELISA in combination with clone MARK-1/MARL-15 (Product code MCA1296P) as detection reagent and purified rat IgE (Product code PRP07A).
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG1 Negative Control:FITC MCA1209F F 0.1 mg loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
AbGUARD® HRP Stabilizer Plus BUF052A C E P WB 100 ml
AbGUARD® HRP Stabilizer Plus BUF052B C E P WB 500 ml
AbGUARD® HRP Stabilizer Plus BUF052C C E P WB 1000 ml
TMB Signal+ BUF054A E 100 ml
TMB Core BUF056A E 100 ml
TMB Core+ BUF062A E 100 ml

Application Based External Images

ELISA

Product Specific References

References for IgE antibody

  1. Negrão-Corrêa, D. et al. (1996) Intestinal transport and catabolism of IgE: a major blood-independent pathway of IgE dissemination during a Trichinella spiralis infection of rats.
    J Immunol. 157 (9): 4037-44.
  2. Bazin, H. et al. (1984) Rat monoclonal antibodies. I. Rapid purification from in vitro culture supernatants.
    J Immunol Methods. 66 (2): 261-9.
  3. Bazin, H. et al. (1974) Transplantable immunoglobulin-secreting tumours in rats. IV. Sixty-three IgE-secreting immunocytoma tumours.
    Immunology. 26 (4): 713-23.
  4. Bazin, H. et al. (1978) Transplantable IgD immunoglobulin-secreting tumors in rat.
    J Immunol. 121 (5): 2077-82.
  5. Bazin, H. et al. (1984) Rat monoclonal antibodies. II. A rapid and efficient method of purification from ascitic fluid or serum.
    J Immunol Methods. 71 (1): 9-16.
  6. Cho, J.K. & Cho, S.W. (2000) Shared epitope for monoclonal IR162 between Anisakis simplex larvae and Clonorchis sinensis and cross-reactivity between antigens.
    J Parasitol. 86 (5): 1145-9.
  7. Silveira, M.R. et al. (2002) Infection with Strongyloides venezuelensis induces transient airway eosinophilic inflammation, an increase in immunoglobulin E, and hyperresponsiveness in rats.
    Infect Immun. 70: 6263-72.
  8. Korinek, M. et al. (2016) Anti-allergic potential of Typhonium blumei: inhibition of degranulation via suppression of PI3K/PLCγ2 phosphorylation and calcium influx.
    Phytomedicine. 23 (14): 1706-15.
  9. Bąbolewska, E. & Brzezińska-błaszczyk, E. (2015) Human-derived cathelicidin LL-37 directly activates mast cells to proinflammatory mediator synthesis and migratory response.
    Cell Immunol. 293 (2): 67-73.
  10. Agier, J. et al. (2018) Cathelicidin LL-37 Affects Surface and Intracellular Toll-Like Receptor Expression in Tissue Mast Cells.
    J Immunol Res. 2018: 7357162.
  11. Ueta, H. et al. (2018) Single blood transfusion induces the production of donor-specific alloantibodies and regulatory T cells mainly in the spleen.
    Int Immunol. 30 (2): 53-67.
  12. Witczak, P. et al. (2020) The Response of Tissue Mast Cells to TLR3 Ligand Poly(I:C) Treatment.
    J Immunol Res. 2020: 2140694.

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