IgE antibody | MARE-1
Mouse anti Rat IgE antibody, clone MARE-1 (MCA193) used to stimulate mast cells in vitro as a positive control prior to ELISA measurement of CCL3 production.
Image caption:
Effect of LL-37 on CCL3 production. For CCL3 measurement, mast cells were incubated with different concentrations of LL-37, anti-IgE at 5 μg/mL (positive control), or medium alone (NS) for 2 h. Results are the mean ± SD of four independent experiments (N=8). Comparisons between groups were carried out by using Student's t-test for small groups. Differences were considered significant at P < 0.05 and are labeled with an asterisk (*) on each graph.
From: Agier J, Brzezińska-Błaszczyk E, żelechowska P, Wiktorska M, Pietrzak J, Różalska S.
Cathelicidin LL-37 Affects Surface and Intracellular Toll-Like Receptor Expression in Tissue Mast Cells.
J Immunol Res. 2018 Feb 19;2018:7357162.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Rat IgE antibody, clone MARE1 (MCA193) used as a challenge in a cytokine release assay for the determination of multiple cytokine production values in vitro.
Image caption:
FcεRI-mediated release of de novo synthesized mediators upon rPMC stimulation with TLR3 ligand. rPMCs were treated with medium alone, poly(I:C) at 10 μg/mL, anti-IgE at 5 μg/mL, or both poly (I:C) and anti-IgE for 2 (a) or 12 h (b–f). CysLT (a), IFN-α (b), IFN-β (c), TNF (d), CCL3 (e), and CXCL8 (f) were measured in cell supernatants using ELISA. Bars for the poly(I:C) at 10 μg/mL and medium alone demonstrate the same data set as in Figure 3. Results are presented as the mean ± SD of 4 independent experiments, and each experiment was done in duplicate (n = 8). ∗p <0.05, ∗∗p <0.01, and ∗∗∗p <0.001; n.s.: not significant.
From: Witczak P, Brzezińska-Błaszczyk E, Agier J.
The Response of Tissue Mast Cells to TLR3 Ligand Poly(I:C) Treatment.
J Immunol Res. 2020 Feb 24;2020:2140694.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
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FN ResetMouse anti Rat IgE
- Product Type
- Monoclonal Antibody
- Clone
- MARE-1
- Isotype
- IgG1
- Specificity
- IgE
Antibody Quality and Performance Guarantee
| Mouse anti Rat IgE antibody, clone MARE-1 recognizes rat epsilon heavy chain of immunoglobulin IgE and does not cross-react with other classes of rat immunoglobulin. Mouse anti Rat IgE antibody, clone MARE-1 binds to rat IgE with an avidity of 4x109M-1 |
- Target Species
- Rat
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by immunoaffinity on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.1% Sodium Azide - Immunogen
- Rat IR162, IR1016, IR2 and IR410 IgE myeloma proteins.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
5ug/ml as coating antibody | |
| Western Blotting | |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- ELISA
- This antibody may be used as a coating antibody in a sandwich ELISA in combination with clone MARK-1/MARL-15 (Product code MCA1296P) as detection reagent and purified rat IgE (Product code PRP07A).
References for IgE antibody
-
Negrão-Corrêa, D. et al. (1996) Intestinal transport and catabolism of IgE: a major blood-independent pathway of IgE dissemination during a Trichinella spiralis infection of rats.
J Immunol. 157 (9): 4037-44. -
Bazin, H. et al. (1984) Rat monoclonal antibodies. I. Rapid purification from in vitro culture supernatants.
J Immunol Methods. 66 (2): 261-9. -
Bazin, H. et al. (1974) Transplantable immunoglobulin-secreting tumours in rats. IV. Sixty-three IgE-secreting immunocytoma tumours.
Immunology. 26 (4): 713-23. -
Bazin, H. et al. (1978) Transplantable IgD immunoglobulin-secreting tumors in rat.
J Immunol. 121 (5): 2077-82. -
Bazin, H. et al. (1984) Rat monoclonal antibodies. II. A rapid and efficient method of purification from ascitic fluid or serum.
J Immunol Methods. 71 (1): 9-16. -
Cho, J.K. & Cho, S.W. (2000) Shared epitope for monoclonal IR162 between Anisakis simplex larvae and Clonorchis sinensis and cross-reactivity between antigens.
J Parasitol. 86 (5): 1145-9. -
Silveira, M.R. et al. (2002) Infection with Strongyloides venezuelensis induces transient airway eosinophilic inflammation, an increase in immunoglobulin E, and hyperresponsiveness in rats.
Infect Immun. 70: 6263-72. -
Korinek, M. et al. (2016) Anti-allergic potential of Typhonium blumei: inhibition of degranulation via suppression of PI3K/PLCγ2 phosphorylation and calcium influx.
Phytomedicine. 23 (14): 1706-15.
View The Latest Product References
-
Bąbolewska, E. & Brzezińska-błaszczyk, E. (2015) Human-derived cathelicidin LL-37 directly activates mast cells to proinflammatory mediator synthesis and migratory response.
Cell Immunol. 293 (2): 67-73. -
Agier, J. et al. (2018) Cathelicidin LL-37 Affects Surface and Intracellular Toll-Like Receptor Expression in Tissue Mast Cells.
J Immunol Res. 2018: 7357162. -
Ueta, H. et al. (2018) Single blood transfusion induces the production of donor-specific alloantibodies and regulatory T cells mainly in the spleen.
Int Immunol. 30 (2): 53-67. -
Witczak, P. et al. (2020) The Response of Tissue Mast Cells to TLR3 Ligand Poly(I:C) Treatment.
J Immunol Res. 2020: 2140694. -
Tulinská, J. et al. (2018) Humoral and cellular immune response in Wistar Han RCC rats fed two genetically modified maize MON810 varieties for 90 days (EU 7th Framework Programme project GRACE).
Arch Toxicol. 92 (7): 2385-99. -
Żelechowska, P. et al. (2022) Different effectiveness of fungal pathogen-associated molecular patterns (PAMPs) in activating rat peritoneal mast cells.
Immunol Lett. Jun 06 [Epub ahead of print].
- RRID
- AB_321899
- UniProt
- P01855
- Entrez Gene
- Ighe
- GO Terms
- GO:0003823 antigen binding
MCA193
148596
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