IgE antibody | MARE-1
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Mouse anti Rat IgE
- Product Type
- Monoclonal Antibody
|Mouse anti Rat IgE antibody, clone MARE-1 recognizes rat epsilon heavy chain of immunoglobulin IgE and does not cross-react with other classes of rat immunoglobulin.
Mouse anti Rat IgE antibody, clone MARE-1 binds to rat IgE with an avidity of 4x109M-1
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by immunoaffinity on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.1% Sodium Azide
- Rat IR162, IR1016, IR2 and IR410 IgE myeloma proteins.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
|ELISA||5ug/ml as coating antibody|
- This antibody may be used as a coating antibody in a sandwich ELISA in combination with clone MARK-1/MARL-15 (Product code MCA1296P) as detection reagent and purified rat IgE (Product code PRP07A).
References for IgE antibody
Negrão-Corrêa, D. et al. (1996) Intestinal transport and catabolism of IgE: a major blood-independent pathway of IgE dissemination during a Trichinella spiralis infection of rats.
J Immunol. 157 (9): 4037-44.
Bazin, H. et al. (1984) Rat monoclonal antibodies. I. Rapid purification from in vitro culture supernatants.
J Immunol Methods. 66 (2): 261-9.
Bazin, H. et al. (1974) Transplantable immunoglobulin-secreting tumours in rats. IV. Sixty-three IgE-secreting immunocytoma tumours.
Immunology. 26 (4): 713-23.
Bazin, H. et al. (1978) Transplantable IgD immunoglobulin-secreting tumors in rat.
J Immunol. 121 (5): 2077-82.
Bazin, H. et al. (1984) Rat monoclonal antibodies. II. A rapid and efficient method of purification from ascitic fluid or serum.
J Immunol Methods. 71 (1): 9-16.
Cho, J.K. & Cho, S.W. (2000) Shared epitope for monoclonal IR162 between Anisakis simplex larvae and Clonorchis sinensis and cross-reactivity between antigens.
J Parasitol. 86 (5): 1145-9.
Silveira, M.R. et al. (2002) Infection with Strongyloides venezuelensis induces transient airway eosinophilic inflammation, an increase in immunoglobulin E, and hyperresponsiveness in rats.
Infect Immun. 70: 6263-72.
Korinek, M. et al. (2016) Anti-allergic potential of Typhonium blumei: inhibition of degranulation via suppression of PI3K/PLCγ2 phosphorylation and calcium influx.
Phytomedicine. 23 (14): 1706-15.
View The Latest Product References
Bąbolewska, E. & Brzezińska-błaszczyk, E. (2015) Human-derived cathelicidin LL-37 directly activates mast cells to proinflammatory mediator synthesis and migratory response.
Cell Immunol. 293 (2): 67-73.
Agier, J. et al. (2018) Cathelicidin LL-37 Affects Surface and Intracellular Toll-Like Receptor Expression in Tissue Mast Cells.
J Immunol Res. 2018: 7357162.
Ueta, H. et al. (2018) Single blood transfusion induces the production of donor-specific alloantibodies and regulatory T cells mainly in the spleen.
Int Immunol. 30 (2): 53-67.
Witczak, P. et al. (2020) The Response of Tissue Mast Cells to TLR3 Ligand Poly(I:C) Treatment.
J Immunol Res. 2020: 2140694.
Tulinská, J. et al. (2018) Humoral and cellular immune response in Wistar Han RCC rats fed two genetically modified maize MON810 varieties for 90 days (EU 7th Framework Programme project GRACE).
Arch Toxicol. 92 (7): 2385-99.
Żelechowska, P. et al. (2022) Different effectiveness of fungal pathogen-associated molecular patterns (PAMPs) in activating rat peritoneal mast cells.
Immunol Lett. Jun 06 [Epub ahead of print].
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"View all Anti-Rat Products
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