IgE antibody | MARE-1

100% Secure


Mouse anti Rat IgE:Biotin

Mouse anti Rat IgE:FITC

Mouse anti Rat IgE:HRP

Mouse anti Rat IgE

Product Type
Monoclonal Antibody
Clone
MARE-1
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA193B E WB 0.5 mg
MCA193F F 0.5 mg
MCA193P E WB 0.5 mg
MCA193 E WB 0.25 mg
Mouse anti Rat IgE antibody, clone MARE-1 recognizes rat epsilon heavy chain of immunoglobulin IgE and does not cross-react with other classes of rat immunoglobulin.

Mouse anti Rat IgE antibody, clone MARE-1 binds to rat IgE with an avidity of 4x109M-1

Product Details

Target Species
Rat
Product Form
Purified IgG conjugated to Biotin - liquid
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on IR2 rat IgE myeloma from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography from tissue culture supernatant
Preparation
Purified IgG prepared by immunoaffinity on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1%Sodium Azide
50%Glycerol
Preservative Stabilisers
0.1%Sodium Azide
50%Glycerol
Preservative Stabilisers
0.01%Thiomersal
50%Glycerol
Preservative Stabilisers
0.1%Sodium Azide
Immunogen
Rat IR162, IR1016, IR2 and IR410 IgE myeloma proteins.
Approx. Protein Concentrations
IgG concentration 1 mg/ml
Approx. Protein Concentrations
IgG concentration 1 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
18 months from date of despatch.
Guarantee
18 months from date of despatch.
Guarantee
18 months from date of despatch.
Guarantee
18 months from date of despatch.

More Information

UniProt
P01855 Related reagents
Entrez Gene
Ighe Related reagents
GO Terms
GO:0003823 antigen binding
Regulatory
For research purposes only

Applications of IgE antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 500ng/ml as detecting antibody
Western Blotting
Flow Cytometry 5ug/ml
ELISA 0.5ug/ml
Western Blotting
ELISA 5ug/ml as coating antibody
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 50ul of the suggested working dilution to label 2 x 107 cells in 100ul.
ELISA
This antibody may be used as a coating antibody in a sandwich ELISA in combination with clone MARK-1/MARL-15 (Product code MCA1296P) as detection reagent and purified rat IgE (Product code PRP07A).

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG1 Negative Control:FITC MCA1209F 0.1 mg F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
AbGUARD® HRP Stabilizer Plus BUF052A 100 ml C E P WB
AbGUARD® HRP Stabilizer Plus BUF052B 500 ml C E P WB
AbGUARD® HRP Stabilizer Plus BUF052C 1000 ml C E P WB
TMB Signal+ BUF054A 100 ml E
TMB Core BUF056A 100 ml E
TMB Core+ BUF062A 100 ml E

Application Based External Images

ELISA

Product Specific References

References for IgE antibody

  1. Negrão-Corrêa, D. et al. (1996) Intestinal transport and catabolism of IgE: a major blood-independent pathway of IgE dissemination during a Trichinella spiralis infection of rats.
    J Immunol. 157 (9): 4037-44.
  2. Bazin, H. et al. (1984) Rat monoclonal antibodies. I. Rapid purification from in vitro culture supernatants.
    J Immunol Methods. 66 (2): 261-9.
  3. Bazin, H. et al. (1974) Transplantable immunoglobulin-secreting tumours in rats. IV. Sixty-three IgE-secreting immunocytoma tumours.
    Immunology. 26 (4): 713-23.
  4. Bazin, H. et al. (1978) Transplantable IgD immunoglobulin-secreting tumors in rat.
    J Immunol. 121 (5): 2077-82.
  5. Bazin, H. et al. (1984) Rat monoclonal antibodies. II. A rapid and efficient method of purification from ascitic fluid or serum.
    J Immunol Methods. 71 (1): 9-16.
  6. Cho, J.K. & Cho, S.W. (2000) Shared epitope for monoclonal IR162 between Anisakis simplex larvae and Clonorchis sinensis and cross-reactivity between antigens.
    J Parasitol. 86 (5): 1145-9.
  7. Silveira, M.R. et al. (2002) Infection with Strongyloides venezuelensis induces transient airway eosinophilic inflammation, an increase in immunoglobulin E, and hyperresponsiveness in rats.
    Infect Immun. 70: 6263-72.
  8. Korinek, M. et al. (2016) Anti-allergic potential of Typhonium blumei: inhibition of degranulation via suppression of PI3K/PLCγ2 phosphorylation and calcium influx.
    Phytomedicine. 23 (14): 1706-15.
  9. Bąbolewska, E. & Brzezińska-błaszczyk, E. (2015) Human-derived cathelicidin LL-37 directly activates mast cells to proinflammatory mediator synthesis and migratory response.
    Cell Immunol. 293 (2): 67-73.
  10. Agier, J. et al. (2018) Cathelicidin LL-37 Affects Surface and Intracellular Toll-Like Receptor Expression in Tissue Mast Cells.
    J Immunol Res. 2018: 7357162.
  11. Ueta, H. et al. (2018) Single blood transfusion induces the production of donor-specific alloantibodies and regulatory T cells mainly in the spleen.
    Int Immunol. 30 (2): 53-67.
Op-in to our ecommunications and set your preferences to start receiving resources, protocols, product updates, special offers, and more.