CD71 antibody | OX-26
FITC conjugated Mouse anti Rat CD71 antibody, clone OX-26 (MCA155F) used for the evaluation of CD71 expression on hair follicle stem cells by flow cytometry.
Image caption:
Flow cytometric analysis of follicle stem cells separated by Vario MACS. (a–d) Single-color flow cytometric analysis of the selected follicle stem cells. X axis represents fluorescence intensity, and Y axis represents cell number. (a) Bulge region cell suspension without antibody staining was used as control. (b) CD34bri cells were isolated by Vario MACS. (c) Control for α6 separation. (d) α6bri cells were isolated by Vario MACS. (e–h) Two-color flow cytometric analysis of follicle stem cells selected by Vario MACS. Cells were divided into four subpopulations: a PEbri FITCdim cells; b PEbri FITCbri cells; c PEdim FITCdim cells; and d PEdim FITCbri cells. (e) Control. (f) CD71 and CD34 was detected with FITC (X axis) and with PE (Y axis). The majority of the isolated cells were CD34briCD71dim cells, characterized by high level expression of CD34 and low level of CD71. No CD34dimCD71bri cells were detected in our experiments. (g) Control for α6 and CD71 combined separation; (h) The α6briCD71dim cells were the main subpopulation of the separated cells.
From: Huang E, Lian X, Chen W, Yang T, Yang L.
Characterization of rat hair follicle stem cells selected by vario magnetic activated cell sorting system.
Acta Histochem Cytochem. 2009 Oct 30;42(5):129-36.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Rat CD71
- Product Type
- Monoclonal Antibody
- Clone
- OX-26
- Isotype
- IgG2a
- Specificity
- CD71
Antibody Quality and Performance Guarantee
| Mouse anti Rat CD71 antibody, clone OX-26 recognizes rat CD71, also known as transferrin receptor, a homodimeric type II transmembrane protein, expressed by all proliferating cells and cells with a requirement for iron, including reticulocytes and capillary endothelium in brain. Clone 0X-26 also binds to a number of non-dividing normal tissues. The balance between a sufficient amount of iron uptake and prevention of accumulation of excess iron within a cell, is vitally important to maintain cellular functions such as oxygen and electron transport and mitochondrial energy metabolism, whilst preventing permanent cell and tissue damage. Transferrin receptor (CD71), transferrin and ferritin have been identified as specialised proteins which control the uptake, transport and storage of free iron in tissues, thereby maintaining iron homeostasis (Crihton et al. 1992). An imbalance in iron homeostasis within the brain has been linked with the neurodegenerative diseases, Alzheimer’s, Parkinson’s, Huntington’s and Multiple Sclerosis (Benarroch 2009). Mouse anti rat CD71 clone OX-26 is reported as suitable for use in immunoelectron microscopy (Lipardi et al. 2002). OX-26 detects a band of ~95kDa in Western blotting under reducing conditions and ~195 kDa under non-reducing conditions reflecting it's homodimeric structure. |
- Target Species
- Rat
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- PHA activated rat lymphocytes.
- Approx. Protein Concentrations
- IgG concentration 1 mg/ml
- Fusion Partners
- Spleen cells from immunized Balb/c mice were fused with cells from the NS1 mouse myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/50 Pack Size: 1 mg 1/10 Pack Size: 0.25 mg |
1/200 Pack Size: 1 mg 1/100 Pack Size: 0.25 mg |
| Immunofluorescence | |
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| Immunohistology - Frozen | |
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| Immunohistology - Paraffin | |
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| Western Blotting | |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG2a Negative Control | MCA1210 | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG2a Negative Control | ||||||
References for CD71 antibody
-
Jefferies, W.A. et al. (1985) Analysis of lymphopoietic stem cells with a monoclonal antibody to the rat transferrin receptor.
Immunology. 54 (2): 333-41. -
Yefimova, M.G. et al. (2002) Impaired retinal iron homeostasis associated with defective phagocytosis in Royal College of Surgeons rats.
Invest Ophthalmol Vis Sci. 43 (2): 537-45. -
Jefferies, W.A. et al. (1984) Transferrin receptor on endothelium of brain capillaries.
Nature. 312 (5990): 162-3. -
Lipardi, C. et al. (2002) Differential recognition of a tyrosine-dependent signal in the basolateral and endocytic pathways of thyroid epithelial cells.
Endocrinology. 143 (4): 1291-301. -
Stevenson, K.S. et al. (2009) Isolation, characterization, and differentiation of thy1.1-sorted pancreatic adult progenitor cell populations.
Stem Cells Dev. 18 (10): 1389-98. -
Jung, S.H. et al. (2008) Plantaris muscle of aged rats demonstrates iron accumulation and altered expression of iron regulation proteins.
Exp Physiol. 93: 407-14. -
Chen, X. et al. (2000) Oxidative damage in an esophageal adenocarcinoma model with rats.
Carcinogenesis. 21: 257-63. -
Huang, E. et al. (2009) Characterization of rat hair follicle stem cells selected by vario magnetic activated cell sorting system.
Acta Histochem Cytochem. 42: 129-36.
View The Latest Product References
-
Petrusca, D.N. et al. (2010) Sphingolipid-mediated inhibition of apoptotic cell clearance by alveolar macrophages.
J Biol Chem. 285: 40322-32. -
Wu, Y.J. et al. (2007) In vivo leukocyte labeling with intravenous ferumoxides/protamine sulfate complex and in vitro characterization for cellular magnetic resonance imaging.
Am J Physiol Cell Physiol. 293: C1698-708. -
De Luca, M.A. et al. (2015) Lactoferrin- and antitransferrin-modified liposomes for brain targeting of the NK3 receptor agonist senktide: Preparation and in vivo evaluation.
Int J Pharm. 479: 129-137. -
Gosk, S. et al. (2004) Targeting anti-transferrin receptor antibody (OX26) and OX26-conjugated liposomes to brain capillary endothelial cells using in situ perfusion.
J Cereb Blood Flow Metab. 24: 1193-204. -
Pang, Z. (2008) Preparation and brain delivery property of biodegradable polymersomes conjugated with OX26.
J Control Release. 128: 120-7. -
Moos, T, Morgan, E.H. (2001) Restricted transport of anti-transferrin receptor antibody (OX26) through the blood-brain barrier in the rat.
J Neurochem. 79: 119-29. -
Moos, T. et al. (2003) Delivery of transferrin and immunoglobulins to the ventricular system of the rat.
Front Biosci. 8: a102-9. -
Fabriek, B.O. et al. (2007) The macrophage CD163 surface glycoprotein is an erythroblast adhesion receptor.
Blood. 109: 5223-9. -
Rathnasamy, G. et al. (2011) Iron and iron regulatory proteins in amoeboid microglial cells are linked to oligodendrocyte death in hypoxic neonatal rat periventricular white matter through production of proinflammatory cytokines and reactive oxygen/nitrogen species.
J Neurosci. 31 (49): 17982-95. -
Loureiro, J.A. et al. (2016) Cellular uptake of PLGA nanoparticles targeted with anti-amyloid and anti-transferrin receptor antibodies for Alzheimer's disease treatment.
Colloids Surf B Biointerfaces. 145: 8-13. -
Loureiro, J.A. et al. (2015) Dual ligand immunoliposomes for drug delivery to the brain.
Colloids Surf B Biointerfaces. 134: 213-9. -
Picard, E. et al. (2015) Targeting iron-mediated retinal degeneration by local delivery of transferrin.
Free Radic Biol Med. 89: 1105-21. -
Chen, C.N. et al. (2023) Restoration of Foxp3(+) Regulatory T Cells by HDAC-Dependent Epigenetic Modulation Plays a Pivotal Role in Resolving Pulmonary Arterial Hypertension Pathology.
Am J Respir Crit Care Med. 208 (8): 879-95.
- Synonyms
- Transferrin Receptor
- RRID
- AB_2201358
- UniProt
- Q99376
- Entrez Gene
- Tfrc
- GO Terms
- GO:0005886 plasma membrane
- GO:0001666 response to hypoxia
- GO:0051087 chaperone binding
- GO:0006953 acute-phase response
- GO:0005739 mitochondrion
- GO:0008233 peptidase activity
- GO:0016021 integral to membrane
- GO:0004998 transferrin receptor activity
- GO:0006508 proteolysis
- View More GO Terms
- GO:0006897 endocytosis
- GO:0007568 aging
- GO:0009986 cell surface
- GO:0010033 response to organic substance
- GO:0010039 response to iron ion
- GO:0010042 response to manganese ion
- GO:0030544 Hsp70 protein binding
- GO:0032526 response to retinoic acid
- GO:0042470 melanosome
- GO:0046688 response to copper ion
- GO:0070062 extracellular vesicular exosome
MCA155G
0515
166480
MCA155R
157411
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