CD68 antibody | ED1
Mouse anti Rat CD68 antibody, clone ED-1 (MCA341GA) used to identify macrophages in formalin fixed, paraffin embedded rat kidney tissue sections by immunohistochemistry.
Image caption:
Decreased interstitial inflammation, increased periglomerular and glomerular macrophages after cessation of Ang II. Ang II increased the influx of interstitial and periglomerular macrophages. After Ang II withdrawal, the number of interstitial ED1 positive cells decreased, but was still elevated versus control rats (A). Periglomerular macrophages slightly increased after Ang II cessation, however this was not signifcant (B). For glomerular macrophages the opposite trend was found, with a decrease of macrophages per glomerulus after Ang II. After stopping infusion, Ang II-infused rats showed an increase in glomerular macrophages (C). Representative photomicrographs of ED-1 positive cells in interstitium (D) and in and around glomeruli (E). (# p<0.05, ## p<0.01, ### p<0.001 vs. control at time of biopsy; **p<0.01, ***p<0.001 vs. Ang II at time of biopsy; $ p<0.05 vs. control at time of sacrifice).
From: Frenay A-RS, Yazdani S, Boersema M, van der Graaf AM, Waanders F, van den Born J, et al. (2015) Incomplete Restoration of Angiotensin II - Induced Renal Extracellular Matrix Deposition and Inflammation Despite Complete Functional Recovery in Rats.
PLoS ONE 10(6): e0129732.
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Mouse anti Rat CD68 antibody, clone ED1 (MCA341R) used for the detection of macrophages in rat liver by immunohistochemistry and western blotting.
Image caption:
Effects of RPE on Kupffer cells activation in liver. (a) Hepatic CD68 expression detected with immunohistology (original magnification, ×400), (b) Hepatic CD68 expression detected with western-blot. Values represent the mean ± SD of three independent experiments. *, versus control; #, versus ethanol.
From: Jing-Hua Peng, Tuan Cui, Zhao-Lin Sun, et al.
Effects of Puerariae Radix Extract on Endotoxin Receptors and TNF- Expression Induced by Gut-Derived Endotoxin in Chronic Alcoholic Liver Injury,
Evidence-Based Complementary and Alternative Medicine, vol. 2012, Article ID 234987, 12 pages, 2012.
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Mouse anti Rat CD68 antibody, clone ED1 (MCA341R) used for the detection of macrophages in rat brain by western blotting.
Image caption:
Western blotting of ED1 (Marker of activated microglia) and TRAIL (tumor necrosis factor-related apoptosis-inducing ligand). Brains were treated with PBS or rAAV2/IL12 and then implanted with tumor on the last day of Week 2 post viral vector injection. The brains were used for Western blotting analysis of the expression of ED1, 34 kDa, and TRAIL, 104 kDa, from microglial cells on the last day of week 1, 2, and 3 following tumor implantation. The brain slices for Western blotting analysis are shown at the bottom. All brains were harvested on the last day of week 1, week 2, and week 3 post tumor implantation. The scale bars indicate 2 mm in brain sections.
From: The treatment of glioblastoma multiforme through activation of microglia and TRAIL induced by rAAV2-mediated IL-12 in a syngeneic rat model. Chiu TL, Wang MJ, Su CC.
J Biomed Sci. 2012 Apr 22;19:45. doi: 10.1186/1423-0127-19-45.
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Mouse anti Rat CD68 antibody, clone ED1 (MCA341GA) used for the detection of macrophages in rat brain by immunohistochemistry.
Image caption:
Immunohistochemistry of ED1 stain in brain sections treated with rAAV2/IL12 (A, B, C, D, n = 4), AAV2/GFP (E, F, n = 2), or PBS (G, H, n = 2), accompanied with tumor implantation; and that in the brain section treated with nothing (n = 1). Immunohistochemistry of brain sections was performed for ED1 on the last day of week 3 after tumor implantation. The brain sections were stained with hematoxylin for nuclei and ED1 for activated microglial cells. The 1st column shows the brain sections pictured before immunostaining; the 2nd column shows the brain sections pictured after staining; the 3rd to 6th columns show pictures taken at four quadrants (black squares in 2nd column) of the tumor adjacent to the normal tissue in the right hemisphere. ED1-positive cells show dark brown. The scale bars indicate 2 mm in 1st and 2nd columns and 100 μm in 3rd-6th columns.
From: Chiu, T.L. Wang, M.J. Su, C.C.
The treatment of glioblastoma multiforme through activation of microglia and TRAIL induced by rAAV2-mediated IL-12 in a syngeneic rat model.
J Biomed Sci. 2012 Apr 22;19:45.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Rat CD68 antibody, clone ED1 (MCA341) used for the detection of macrophages in rat brain by immunohistochemistry.
Image caption:
Immunohistochemical stains of ED1 and TRAIL in brain sections treated with AAV2/IL12 (A, B) or PBS (C, D), accompanied with tumor implantation; and that in the brain treated with nothing (E). Brain sections used in Figure 4 were also stained with hematoxylin for nuclei, ED1, and TRAIL, and pictured as in Figure 4. The 1st and 2nd columns show the low and high power fields of ED1 stain, respectively; the 3rd and 4th columns show the low and high power fields of TRAIL stain. Cells stained with ED1 or TRAIL show dark brown. The scale bars indicate 100 μm in low power field and 50 μm in high power field.
From: Chiu, T.L. Wang, M.J. Su, C.C.
The treatment of glioblastoma multiforme through activation of microglia and TRAIL induced by rAAV2-mediated IL-12 in a syngeneic rat model.
J Biomed Sci. 2012 Apr 22;19:45.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Rat CD68 antibody, clone ED1 (MCA341GA) used for the detection of macrophages in rat brain by flow cytometry.
Image caption:
Fluorescence-activated cell sorting analysis of cells isolated from adjuvant-induced arthritis rat synovial tissue. Twenty-four hours after systemic administration of N-(2-hydroxypropyl)methacrylamide copolymer labeled with Alexa Fluor® 488 (P-Alexa), cells were isolated from adjuvant-induced arthritis rat synovial tissue for fluorescence-activated cell sorting (FACS) analysis. IgG1 was used as control for CD68 and prolyl-4-hydroxylase. IgG2a was used as control for CD11c. The result for IgG2a is similar to that for IgG1 with the upper right quadrant detected at 2.45% (data not shown).
From: Quan, L.D. et al. 2010
Development of a macromolecular prodrug for the treatment of inflammatory arthritis: mechanisms involved in arthrotropism and sustained therapeutic efficacy.
Arthritis Research & Therapy 2010 12:R170.
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Mouse anti Rat CD68 antibody, clone ED1 (MCA341) used for the detection of macrophages in rat joints by immunofluorescence.
Image caption:
Immunohistochemical staining of sections from inflamed joints with anti-CD68 or anti-prolyl-4-hydroxylase antibodies. Representative confocal microscopic images of (a) anti-CD68 and (b) anti-prolyl-4-hydroxylase immunohistochemical staining of decalcified, Alexa Fluor® 488-labeled N-(2-hydroxypropyl)methacrylamide copolymer (P-Alexa)-treated adjuvant-induced arthritis (AA) rat ankle joint sections are summarized in this figure. Each panel is composed of four sub-images: antibody red staining, P-Alexa green signal, differential interference contrast (DIC) image and the co-localization of the three. The co-localization of red and green color in both panels yields a yellow color, which confirms the uptake of the HPMA copolymer conjugate by CD68-positive (macrophage-like) or prolyl-4-hydroxylase-positive (fibroblast-like) synoviocytes in AA rat ankle joints.
From: Quan, L.D. et al. Arthritis Research & Therapy 2010 12:R170.
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Mouse anti Rat CD68 antibody, clone ED1 (MCA341GA) used for the detection of macrophages in rat brain by immunofluorescence.
Image caption:
Immunohistochemical stains of ED1 and TRAIL in brain sections treated with AAV2/IL1. Immunohistochemistry for CD68 in the optic nerve. There is a cellular accumulation indicated by DAPI at the crushed sites of the optic nerve. Some of the cells are positively stained with CD68 suggesting that there is a recruitment of microglia/macrophages to the crushed sites. Bar = 100 μm.
From: Suzuki H, Oku H, Horie T, Morishita S, Tonari M, et al. (2014)
Changes in Expression of Aquaporin-4 and Aquaporin-9 in Optic Nerve after Crushing in Rats.
PLoS ONE 9(12): e114694.
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Mouse anti Rat CD68 antibody, clone ED1 (MCA341R) used for the detection of macrophages in rat optic nerve by immunofluorescence.
Image caption:
Immunohistochemical stains of ED1 and TRAIL in brain sections treated with AAV2/IL1. Immunohistochemistry for CD68 in the optic nerve. There is a cellular accumulation indicated by DAPI at the crushed sites of the optic nerve. Some of the cells are positively stained with CD68 suggesting that there is a recruitment of microglia/macrophages to the crushed sites. Bar = 100 μm.
From: Suzuki H, Oku H, Horie T, Morishita S, Tonari M, et al. (2014)
Changes in Expression of Aquaporin-4 and Aquaporin-9 in Optic Nerve after Crushing in Rats.
PLoS ONE 9(12): e114694.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Rat CD68 antibody, clone ED1 (MCA341) used for the detection of macrophages in rat optic nerve by immunofluorescence.
Image caption:
Immunohistochemical stains of ED1 and TRAIL in brain sections treated with AAV2/IL1. Immunohistochemistry for CD68 in the optic nerve. There is a cellular accumulation indicated by DAPI at the crushed sites of the optic nerve. Some of the cells are positively stained with CD68 suggesting that there is a recruitment of microglia/macrophages to the crushed sites. Bar = 100 μm.
From: Suzuki H, Oku H, Horie T, Morishita S, Tonari M, et al. (2014)
Changes in Expression of Aquaporin-4 and Aquaporin-9 in Optic Nerve after Crushing in Rats.
PLoS ONE 9(12): e114694.
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Mouse anti Rat CD68 antibody, clone ED1 (MCA341GA) used for the detection of CD68 expressing macrophages in the spleen of liposome treated rats by immunofluorescence on formalin fixed cryosections.
Image caption:
Confirmation of Macrophage Cell Depletion Examined in the Spleens of Liposome-Treated Animals. A-B) 1 week after liposome administration no macrophage cells were present in the spleens of treated animals, as confirmed by the absence of CD68 (macrophage marker) immunoreactivity (***P <0.0001; I). C-D) By 2 week after liposome treatment completion, macrophage cells (CD68+) had already began to repopulate in the spleen of treated animals (*P <0.05; I). E-F) At the end of the experimental period (day 35) macrophage numbers in the spleen of treated animals had returned to normal levels, as compared to untreated animals (G-H). I) Quantification of immunoreactive CD68+ cells as shown in panels A-H, demonstrated a significant cellular reduction during liposome administration and during repopulation 2 weeks after treatment completion. No differences in macrophage cells were found at the end of the study. Columns represent an averaged mean (5 sections per animal, n = 5) and error bars indicate error of mean (+/- S.E.). Scale bars A, C, E, G 500 μm, enlarged views B, D, F, H 200 μm.
From: Salegio EA, Pollard AN, Smith M, Zhou XF. Macrophage presence is essential for the regeneration of ascending afferent fibres following a conditioning sciatic nerve lesion in adult rats.
BMC Neurosci. 2011 Jan 20;12:11.
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Mouse anti Rat CD68 antibody, clone ED1 (MCA341R) used for the detection of CD68 expressing macrophages in rat kidney by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Renal ED-1 immunostaining of the rats treated for 28 days: sham (A), losartan (B), leptin (C) and leptin plus losartan (D). ED-1-positive cell quantification (in cells/area) (E). Magnification of the leptin-treated rats ED-1 positive cell area. G = glomeruli; arrowheads = ED-1-positive cells; bar = 100 μm. *p<0.05 versus the sham; #p<0.05 versus the leptin-treated rats and $p<0.05 versus the losartan-treated. For the rats treated for 28 days: sham (n = 6); losartan (n = 6), leptin (n = 8); leptin plus losartan (n = 6).
From: Thieme, K. and Oliveira-Souza, M. (2015)
Renal Hemodynamic and Morphological Changes after 7 and 28 Days of Leptin Treatment: The Participation of Angiotensin II via the AT1 Receptor.
PLoS ONE 10(3): e0122265.
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Mouse anti Rat CD68 antibody, clone ED-1 (MCA341R) used for the identification of CD68 expressing cells in rat kidney by immunohistochemistry
Image caption:
ED-1 stain of kidney tissue. The groups are (A) control, (B) EG-exposed, (C) EG+STS-exposed, (D) EG+SC-exposed, (E) EG+SS-exposed animal groups. The ED-1 stain was markedly increased in EG-exposed animals but decreased in all treatment groups. The significance levels are with reference to the EG group. The data are means ± SD from 7–8 animals per group. ***p < 0.001.
From: Bijarnia RK, Bachtler M, Chandak PG, van Goor H, Pasch A (2015)
Sodium Thiosulfate Ameliorates Oxidative Stress and Preserves Renal Function in Hyperoxaluric Rats. PLoS ONE 10(4): e0124881.
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Mouse anti Rat CD68 antibody, clone ED1 (MCA341R) used for the identification of macrophages in venous thrombi by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Effects of Temporary Dietary Iron Restriction on Leukocyte Recruitment, Neovascularization, and Plasmin System of Stasis-Induced Venous Thrombi in Rats. (A) Representative images of CD68 staining of the venous thrombi. Scale bars: 100 μm. (B) Quantification of CD68 positive cells in the venous thrombi of the DVT and DVT+IR groups (n = 6 in each group). (C) Representative images of vWF staining of the venous thrombi. Scale bars: 50 μm. Arrows show vascular channels. (D) Quantification of vascular channels in the venous thrombi of the DVT and DVT+IR groups (n = 6 in each group). The number of CD68 positive cells or vascular channels in 10 randomly selected high power fields (HPF) was counted in a blinded manner. (E) CD31 protein expression (top; representative gel blots depicting expression of CD31 and GAPDH, bottom; relative levels of expression) in the venous thrombi of the DVT and DVT+IR groups (n = 5 in each group). (F) Intrathrombotic gene expression of uPA, tPA, PAI-1, and fibrinogen α chain in the DVT and DVT+IR groups (n = 4 in each group). *p < 0.05 versus the DVT group.
From: Oboshi M, Naito Y, Sawada H, Hirotani S, Iwasaku T, Okuhara Y, et al. (2015)
Temporary Dietary Iron Restriction Affects the Process of Thrombus Resolution in a Rat Model of Deep Vein Thrombosis.
PLoS ONE 10(5): e0126611.
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Mouse anti Rat CD68 antibody, clone ED-1 (MCA341GA) used for the detection of infiltrating macrophages in lung tissue by immunofluorescence.
Image caption:
IH causes the accumulation of macrophages and upregulates β3AR expression in the lungs. (A) Representative bright-field images of lung sections from the N and IH rats and images of immunofluorescent staining of such sections with anti-ED-1 antibody, anti-β3AR antibody, or both (merged images). Calibration bar = 200 μm for 40 x, 50 μm for 200 x. (B, C) The numbers of ED-1- and β3AR-positive cells per field (200 x) were counted using Image Pro Plus ver. 4.1 (n = 6 each, mean ± S.D.) (D) Ratio of the percentage of β3AR-positive cells to the percentage of ED-1-positive cells (n = 6 each, mean ± S.D.) (E) Representative images of double immunocytochemical staining using anti-ED-1 and β3AR antibody in BALF-derived macrophages. Calibration bar = 50 μm. (F) Western blot analysis of β3AR in lung homogenate solutions from the N and IH rats (n = 6 each, mean ± S.D.) (G) The expression level of β3AR mRNA in lung tissue samples from the N and IH rats (n = 6 each, mean ± S.E.M.) (H) Western blot analysis of β3AR in BALF-derived pulmonary macrophages obtained after 6 weeks of IH or normoxic exposure (n = 5 each, mean ± S.D.) *Significant difference between the N and IH rats (*P<0.05, **P<0.01).
From: Nagai H, Kuwahira I, Schwenke DO, Tsuchimochi H, Nara A, Ogura S, et al. (2015) Pulmonary Macrophages Attenuate Hypoxic Pulmonary Vasoconstriction via β3AR/iNOS Pathway in Rats Exposed to Chronic Intermittent Hypoxia.
PLoS ONE 10(7): e0131923.
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Mouse anti Rat CD68 antibody, clone ED-1 (MCA341GA) used for the detection of infiltrating macrophages in lung tissue by immunofluorescence.
Image caption:
Pulmonary macrophages in IH rats expressed pro-inflammatory markers including iNOS. The pulmonary macrophages were obtained from BALF after 6 weeks of IH or normoxic exposure. (A) Immunocytochemical staining of pro-inflammatory markers iNOS, CD11c, and IL-6 was performed. (B) Western blot analysis of iNOS, IL-6, and TNFα in BALF-derived macrophages (n = 5 each, mean ± S.D.). *Significant difference between the N and IH rats (*P<0.05).
From: Nagai H, Kuwahira I, Schwenke DO, Tsuchimochi H, Nara A, Ogura S, et al. (2015)
Pulmonary Macrophages Attenuate Hypoxic Pulmonary Vasoconstriction via β3AR/iNOS Pathway in Rats Exposed to Chronic Intermittent Hypoxia.
PLoS ONE 10(7): e0131923.
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Mouse anti Rat CD68 antibody, clone ED-1 (MCA341GA) used for the detection of infiltrating macrophages in lung tissue by immunofluorescence.
Image caption:
Acute intratracheal administration of clodronate restores HPV in IH rats. (A) Representative bright-field images and images of immunofluorescent staining using anti-ED-1 antibody of lung sections with or without clodronate. Clodronate (500 μg of clodronate in 100 μL of saline) was injected intratracheally just after the end of the 6-week IH/normoxia exposure period. Calibration bar = 200 μm. (B) Representative microangiographic images of the small pulmonary arteries in the N and IH rats obtained 3 days after the i.t. administration of clodronate. The black arrows point to branches that underwent vasoconstriction. (C) Relationship between vessel size and the extent of the pulmonary vasoconstriction induced in response to acute hypoxia. Data are presented as mean ± S.E.M. values. *Significant change in vessel diameter compared with the baseline conditions (**P<0.01).
From: Nagai H, Kuwahira I, Schwenke DO, Tsuchimochi H, Nara A, Ogura S, et al. (2015)
Pulmonary Macrophages Attenuate Hypoxic Pulmonary Vasoconstriction via β3AR/iNOS Pathway in Rats Exposed to Chronic Intermittent Hypoxia.
PLoS ONE 10(7): e0131923.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Rat CD68 antibody, clone ED1 (MCA341R) used for the identification of macrophages in rat prostate ventral lobe by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
(A) Representative sections from the ventral prostate lobe of Dunning tumor-bearing and control rats stained to visualize CD68+ macrophages (brown, 200X magnifications, T; tumor). (B) Volume density of CD68+ macrophages in the tumor-adjacent prostate tissue (TINT) and in controls. a; significantly different than controls, b; large G tumors significantly different than small G tumors, and c; significantly different than corresponding tumor at day 7, P<0.05, n = 5–13. (C) Scatterplot of the volume density of CD68+ macrophages in the tumor-bearing organ plotted against tumor weight (correlation coefficients are given in the result text).
From: Adamo HH, Strömvall K, Nilsson M, Halin Bergström S, Bergh A (2015)
Adaptive (TINT) Changes in the Tumor Bearing Organ Are Related to Prostate Tumor Size and Aggressiveness.
PLoS ONE 10(11): e0141601.
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Mouse anti Rad CD68 antibody, clone ED1 (MCA341GA) used for the identification of macrophages in mouse retina by immunohistochemistry on formalin fixed, paraffin embedded tissue sections in a model of Neuromyelitisoptica/ spectrum disorder (NMO/SD)
Image caption:
Histological characterization of inflammatory lesions in the retina. Consecutive retinal sections were reacted with antibodies against CD3, ED1, rat IgG, AQP4, GFAP, glutamine synthetase (GS), C9, and human IgG. Positive reaction products are shown in brown or red (C9 only). All sections were counterstained with hematoxylin to reveal nuclei in blue. Bars = 100 μm. The tissue derived from animals injected with AQP4268–285-specific T cells and NMO-IgG (ENMO), with AQP4268–285-specific T cells and human control IgG (T + co IgG) and healthy controls, and show inflammation at the inner blood retinal barrier (IBRB), at the outer blood retinal barrier (OBRB), simultaneously at both retinal barriers (I/OBRB), or an absence of inflammation at these sites in the control animals. The insert shown for the GS stained retina of the rat injected with T cells and co IgG refers to the area labeled by a white star in the adjacent overview. It demonstrates that there is no loss of GS reactivity at this site, but the lumen of a blood vessel. Bar = 100 μm.
From: Zeka B, Hastermann M, Kaufmann N, Schanda K, Pende M, Misu T, Rommer P, Fujihara K, Nakashima I, Dahle C, Leutmezer F, Reindl M, Lassmann H, Bradl M.
Aquaporin 4-specific T cells and NMO-IgG cause primary retinal damage in experimental NMO/SD.
Acta Neuropathol Commun. 2016 Aug 8;4(1):82.
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Mouse anti RatCD68 antibody, clone ED1 (MCA341GA) used for the identification of microglia in rat brain following traumatic brain injury using immunohistochemistry. Lymphocytes (a + b) are identified using Mouse anti Rat CD43 antibody, clone W3/13 (MCA54G) in the same experiment.
Image caption:
AhR double labeling in brain sections from day 1 after TBI. a Most AhR+ non-neuronal cells (brown) co-expressed W3/13 (blue). The boxed areas indicate the regions that were further observed under high-power magnification shown in (b). c and d: However, most AhR+ non-neuronal cells (blue) did not co-localize with ED1+ microglia (brown, c) or GFAP+ astrocytes (brown, d). Scale bar = 100 μm for (a, c) and d; 50 μm for (b)
From: Xu K, Yang Z, Shi R, Luo C, Zhang Z.
Expression of aryl hydrocarbon receptor in rat brain lesions following traumatic brain injury.
Diagn Pathol. 2016 Aug 9;11(1):72.
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Mouse anti Rat CD68 (MCA341GA) used to identify microglia in nanowire neural implants in rat striatum by immunoflurescence.
Image caption:
Inflammatory tissue response, cell nuclei and neuronal density (inner ROI). Quantification in the inner ROI (0–50 μm) of ED1-positive area (a), GFAP-positive area (b), NeuN density (c), and cell nuclei density (d) at 1 year for HfOx-coated GaP nanowires (biostable) and SiOx-coated GaP nanowires (degradable). The boxes correspond to median values with indication of the 25 and 75 percentiles, and the whiskers show the minimum and maximum values. Mann–Whitney test was used. Representative fluorescent images of the tissue response 1 year post injection of e 2 μm long HfOx-coated nanowires (biostable) and f 2 μm long SiOx-coated GaP nanowires (degradable). ED1-positive cells (green), GFAP-positive cells (red) and cell nuclei (blue). Scale bar 100 μm.
From: Gällentoft L, Pettersson LM, Danielsen N, Schouenborg J, Prinz CN, Linsmeier CE.
Impact of degradable nanowires on long-term brain tissue responses.
J Nanobiotechnology. 2016 Aug 9;14(1):64.
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Mouse anti Rat CD68 (MCA341GA) used to identify microglia in nanowire neural implants in rat striatum by immunoflurescence.
Image caption:
Confocal images 1 year post nanowire injection. Representative laser scanning confocal microscopy images of the scar area after injection of 2 μm long HfOx-coated (a–e) and SiOx-coated GaP (f–j) nanowires 1 year post injection. The intact HfOx-coated and fragmented SiOx-coated GaP nanowires are visualized in white using scattered laser light mode. The images demonstrate the difference in nanowire cell load and degradability of the two types of nanowires studied. Note also the few rod-shaped SiOx-coated nanowires found in single, small ED1-positive cells (arrows). ED1-positive cells (green), GFAP-positive cells (red), cell nuclei (blue) and nanowires (white, scattered laser light). Scale bar 20 μm.
From: Gällentoft L, Pettersson LM, Danielsen N, Schouenborg J, Prinz CN, Linsmeier CE.
Impact of degradable nanowires on long-term brain tissue responses.
J Nanobiotechnology. 2016 Aug 9;14(1):64.
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Mouse anti Rat CD68 (MCA341GA) used to identify microglia in nanowire neural implants in rat striatum by immunoflurescence.
Image caption:
Close-up confocal images. Merged close-up of laser scanning confocal microscopy images showing ED1-positive cells in the scar area after injection of 2 μm long HfOx-coated (a) and SiOx-coated GaP (b) nanowires 1 year post injection. The images show internalization of intact HfOx-coated and degraded SiOx-coated GaP nanowires in microglia/macrophages. ED1-positive cells (green), GFAP-positive cells (red), cell nuclei (blue) and nanowires (white, scattered laser light). Scale bar 5 μm.
From: Gällentoft L, Pettersson LM, Danielsen N, Schouenborg J, Prinz CN, Linsmeier CE.
Impact of degradable nanowires on long-term brain tissue responses.
J Nanobiotechnology. 2016 Aug 9;14(1):64.
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Mouse anti Rat CD68 antibody, clone ED-1 (MCA341R) used for the demonstration of spinal cord infiltrating macrophages in a rat model of multiple sclerosis by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Spinal cord (SC) histopathology. Representative examples of ED1-staining in animal at 4 days post immunization (dpi) shows no abnormalities (a); small level of inflammatory infiltration at 11d pi (b); in contrast heavily infiltrated SC section at 14 dpi (c). Representative Luxol Fast Blue-stained cross-section of the SC shows intact myelin (blue) in animal at 4 dpi (d) only small areas of demyelination, with mainly intact myelin (blue) on 11 dpi (e); a cross-section from diseased animal on day 14 dpi (f) shows extensively demyelinated areas (purple)
From:Herold S, Kumar P, Jung K, Graf I, Menkhoff H, Schulz X, Bähr M, Hein K.
CatWalk gait analysis in a rat model of multiple sclerosis.
BMC Neurosci. 2016 Nov 30;17(1):78.
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Mouse anti Rat CD68 antibody, clone ED-1 (MCA341R) used for the identification of monocytic cells in rat brain by immunofluorescence on vibratome sections.
Image caption:
The influx of monocytes into the injured brain after mTBI.
In these experiments, the depth of brain deformation was set at 1 mm, and the images shown were acquired at 24 h post-mTBI. Double immunostaining of injured brains was performed with anti-CD68 antibody, a monocyte marker, and an antibody to von Willebrand factor (vWF), a marker for endothelial cells. (A, B) Similar to severe TBI, in mTBI, monocytes did not appear in the injured brain until 24 h after the impact. However, only a small number of monocytes invaded the injured brain parenchyma at 24 h post-mTBI. Note that at this time point after injury, these inflammatory cells were predominantly located close to intraparenchymal blood microvessels. (C, D) In contrast to brain parenchyma, a large number of monocytes crossed the pial microvessels and invaded the subarachnoid space (SAS) near the injury site. Note that some of these monocytes appeared to subsequently move along the perivascular space to enter the brain parenchyma (arrows). (E–G) An influx of monocytes into the ipsilateral cistern of velum interpositum (CVI) also occurred at 24 h post-mTBI. Bars: panels A, B, E, 100 μm; panels C, D, F, G, 50 μm.
From: Szmydynger-Chodobska J, Shan R, Thomasian N, Chodobski A (2016)
The Involvement of Pial Microvessels in Leukocyte Invasion after Mild Traumatic Brain Injury.
PLoS ONE 11(12): e0167677.
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Mouse anti Rat CD68 antibody, clone ED1 (MCA341) used for the detection of CD68 expressing cells in rat brain by immunofluorescence on free-floating sections.
Image caption:
Implantation mode and embedding dependent glial reactions. A) Representative images of rigid, flex and flex embedded mode implantations after 6 weeks, showing mode dependent alterations in astrocytosis, as shown in red (GFAP), microglia responses, as shown in green (ED1) and merged (DAPI in blue (total cell nuclei)). Scale bar: 50 μm. Cephalocaudal direction corresponds to top-to-bottom in image. B and C) Quantification of the GFAP and ED1 densities, respectively, that surrounds the different implantation sites in the inner ROI (0–50 μm). The box corresponds to the 25th and 75th percentiles, the median value is indicated by the horizontal line within each box, and the whiskers show the minimum and maximum values. The horizontal lines indicate statistical differences.
From: Köhler P, Wolff A, Ejserholm F, Wallman L, Schouenborg J, et al. (2015)
Influence of Probe Flexibility and Gelatin Embedding on Neuronal Density and Glial Responses to Brain Implants.
PLoS ONE 10(3): e0119340.
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Mouse anti Rat CD68 antibody, clone ED1 (MCA341GA) used for the identification of macrophages in the spleen of rats by immunofluorescence
Image caption:
anti-CD20, anti-CD3, and anti-CD68 immunofluorescence costaining of rat spleen follicle. (A) shows anti-CD20-positive B cells in the B cell zone of a splenic follicle (green), anti-CD3-positive T cells in T cell zone of splenic follicle (red), and CD68-positive macrophages (yellow) in the splenic red pulp.
From: Kühne L, Jung B, Poth H, Schuster A, Wurm S, Ruemmele P, Banas B, Bergler T.
Renal allograft rejection, lymphocyte infiltration, and de novo donor-specific antibodies in a novel model of non-adherence to immunosuppressive therapy.
BMC Immunol. 2017 Dec 19;18(1):52.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Rat CD68 antibody, clone ED-1 (MCA341R) used for the identification in rat pulmonary macrophages by immunohistochemistry on formalin fixed paraffin embedded lung sections.
Image caption:
Quantification of ED1-positive cells (A) revealed significantly increased numbers of monocytes/macrophages in CDH lungs. Immunofluorescence showed an accumulation of ED1-positve cells around pulmonary vessels in nitrofen-exposed CDH lungs (C) compared to controls (B). Statistical analysis by Student's t-test, *p<0.05, **p<0.01, p***<0.001, Scale bar 50μm.
From: Gosemann J-H, Friedmacher F, Hofmann A, Zimmer J, Kuebler JF, Rittinghausen S, et al. (2018)
Prenatal treatment with rosiglitazone attenuates vascular remodeling and pulmonary monocyte influx in experimental congenital diaphragmatic hernia.
PLoS ONE 13(11): e0206975.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Rat CD68 antibody, clone ED-1 (MCA341R) used for the demonstration of macrophages in rat brain cryosections by immunofluorescence.
Image caption:
Sodium butyrate diminishes the generation of microglial/macrophage cells in the ipsilateral hemisphere after HI. Neonatal HI was induced at postnatal day 7. SB was administered directly after the onset of HI and for five consecutive days. BrdU was administered daily for three consecutive days starting 4 days after the onset of HI. Sections from SB-treated as well as untreated ipsilateral rat hemispheres were double-labeled with anti-BrdU antibody (green) and ED1 antibody (marker for microglia and macrophages) (red) 14 days after HI. a Confocal photomicrographs of brain sections show double-labeled cells (yellow). Enlargements present areas marked in rectangles. b Number of BrdU/ED1-positive cells quantified in the ipsilateral hemisphere area (0.36 mm2). The values are mean ± SD from five animals per group. One-way ANOVA and Bonferroni test indicate significant differences between investigated groups, ***p <0.001 Abbreviation: ipsi—ipsilateral.
From: Jaworska J, Zalewska T, Sypecka J, Ziemka-Nalecz M.
Effect of the HDAC Inhibitor, Sodium Butyrate, on Neurogenesis in a Rat Model of Neonatal Hypoxia-Ischemia: Potential Mechanism of Action.
Mol Neurobiol. 2019 Sep;56(9):6341-70..
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Rat CD68 antibody, clone ED-1 (MCA341R) used for the demonstration of macrophages in rat brain cryosections by immunofluorescence.
Image caption:
Sodium butyrate reduces microglial/macrophage cell number in the rat ipsilateral hemisphere after HI. Neonatal HI was induced at postnatal day 7. SB was administered directly after the onset of HI and for five consecutive days. Sections from SB-treated as well as untreated ipsilateral rat hemispheres were labeled with ED1 (marker for microglia and macrophages) antibody 14 days after HI. a Confocal photomicrographs of ipsilateral hemispheres show a large number of ED1-stained cells after HI and marked reduction after SB treatment. Enlargements present areas marked in rectangles. b Number of ED1-positive cells quantified in the whole ipsilateral hemisphere. The values are means ± SD from five animals per group. One-way ANOVA and Bonferroni test indicate significant differences between investigated groups, *p <0.05. Abbreviation: ipsi—ipsilateral.
From: Jaworska J, Zalewska T, Sypecka J, Ziemka-Nalecz M.
Effect of the HDAC Inhibitor, Sodium Butyrate, on Neurogenesis in a Rat Model of Neonatal Hypoxia-Ischemia: Potential Mechanism of Action.
Mol Neurobiol. 2019 Sep;56(9):6341-70..
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Rat CD68 antibody, clone ED-1 (MCA341R) used for the demonstration of macrophages in rat brain cryosections by immunofluorescence.
Image caption:
Sodium butyrate promotes the polarization of microglia from M1 to M2-like phenotype after HI. Neonatal HI was induced at postnatal day 7. SB was administered directly after the onset of HI and for five consecutive days. Sections from ipsilateral hemispheres 14 days after HI were stained for anti-ED1 antibody (red); and for anti-IL-1β, marker for M1 phenotype (green) (a); Nuclei were labeled with the Hoechst dye (blue). Enlargement of areas marked in rectangles shows respective double-labeled cells (yellow). Number of IL-1β/ED1-positive cells (c) quantified in the ipsilateral hemisphere area (0.36 mm2). The values are means ± SD from five animals per group. One-way ANOVA and Bonferroni test indicate significant differences between investigated groups, **p <0.01 and ***p <0.001. Abbreviation: ipsi—ipsilateral.
From: Jaworska J, Zalewska T, Sypecka J, Ziemka-Nalecz M.
Effect of the HDAC Inhibitor, Sodium Butyrate, on Neurogenesis in a Rat Model of Neonatal Hypoxia-Ischemia: Potential Mechanism of Action.
Mol Neurobiol. 2019 Sep;56(9):6341-70..
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Rat CD68 antibody, clone ED1 (MCA341R) used for the demonstration of macrophages by immunofluorescence on acetone fixed cardiac cryosections post ischemia/reperfusion.
Image caption:
Effects of Lebetin 2 (L2) and B-type natriuretic peptide (BNP) on post-ischemic macrophage recruitment. Myocardial infarction was induced by 35 min of ischemia followed by a 2-day reperfusion period. (a) Infiltrated macrophages were assessed by CD68 immunolabeling in the infarcted area and its border zone (viable area), as well as in the septum (non-ischemic area) of rats treated with saline (1 μL/g), BNP (50 ng/g) or L2 (100 ng/g) intraperitoneally, 5 min before reperfusion. On each section, several fields were photographed (12–30 images/rat, n = 5–6) and the mean number of macrophages per field was calculated; (b) representative microphotographs showing the variable density of macrophages (CD68 in green and nuclei in blue) in the infarcted area from control and treated animals. Data are mean ± SEM. *, p <0.05, ***, p <0.001 vs. saline (control) corresponding group, $, p <0.05 vs. BNP corresponding group, ✝✝✝, p <0.001 vs. corresponding other LV areas. Scale bars = 50μm. ×20. DAPI, 4′, 6′-diamidino-2-phenylindole.
From: Tourki B, Dumesnil A, Belaidi E, Ghrir S, Godin-Ribuot D, Marrakchi N, Richard V, Mulder P, Messadi E.
Lebetin 2, a Snake Venom-Derived B-Type Natriuretic Peptide, Provides Immediate and Prolonged Protection against Myocardial Ischemia-Reperfusion Injury via Modulation of Post-Ischemic Inflammatory Response.
Toxins (Basel). 2019 Sep 10;11(9). pii: E524.
doi: 10.3390/toxins11090524.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Rat CD68 antibody, clone ED1 (MCA341R) used to detect macrophages in kidneys of rats by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Insert image caption
From: Nozomu Abe, Hiroaki Toyama, Kazutomo Saito, et al.
Delayed Rectifier K+-Channel Is a Novel Therapeutic Target for Interstitial Renal Fibrosis in Rats with Unilateral Ureteral Obstruction,
BioMed Research International, vol. 2019, Article ID 7567638, 11 pages, 2019.
doi: 10.1155/2019/7567638.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Rat CD68 antibody, clone ED-1 (MCA341R) used for the demonstration of macrophages in rat brain cryosections by immunofluorescence.
Image caption:
Sodium butyrate promotes the polarization of microglia from M1 to M2-like phenotype after HI. Neonatal HI was induced at postnatal day 7. SB was administered directly after the onset of HI and for five consecutive days. Sections from ipsilateral hemispheres 14 days after HI were stained for anti-ED1 antibody (red); and for arginase-1 (Arg-1), marker specific for M2 phenotype (green) (a); Nuclei were labeled with the Hoechst dye (blue). Enlargement of areas marked in rectangles shows respective double-labeled cells (yellow). Number of Arg-1/ED1-positive cells (d) quantified in the ipsilateral hemisphere area (0.36 mm2). The values are means ± SD from five animals per group. One-way ANOVA and Bonferroni test indicate significant differences between investigated groups, **p <0.01 and ***p <0.001. Abbreviation: ipsi—ipsilateral.
From: Jaworska J, Zalewska T, Sypecka J, Ziemka-Nalecz M.
Effect of the HDAC Inhibitor, Sodium Butyrate, on Neurogenesis in a Rat Model of Neonatal Hypoxia-Ischemia: Potential Mechanism of Action.
Mol Neurobiol. 2019 Sep;56(9):6341-70..
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Rat CD68 antibody, clone ED-1 (MCA341R) used for the detection of infiltrating macrophages in lung tissue by immunofluorescence.
Image caption:
eNOS and nNOS are not upregulated in BALF-derived macrophages in IH rats. (A) Representative images of double immunocytochemical staining with anti-ED-1, and eNOS or nNOS antibody in BALF-derived macrophages. Calibration bar = 50 μm. (B) Western blot analysis of eNOS in BALF-derived macrophages (n = 5 each, mean ± S.D.) nNOS was undetectable in both N and IH rats.
From: Nagai H, Kuwahira I, Schwenke DO, Tsuchimochi H, Nara A, Ogura S, et al. (2015)
Pulmonary Macrophages Attenuate Hypoxic Pulmonary Vasoconstriction via β3AR/iNOS Pathway in Rats Exposed to Chronic Intermittent Hypoxia.
PLoS ONE 10(7): e0131923.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Rat CD68 antibody, clone ED1 (MCA341GA) used for the detection of macrophages in rat skin by immunohistochemistry.
Image caption:
Histological staining of control and HBOT wounds at post-wounding days 7 and 29. A–D) H&E staining. E–H) CD34 immunohistochemistry. I+J) CD68 immunohistochemistry.
From: Tuk B, Tong M, Fijneman EMG, van Neck JW (2014)
Hyperbaric Oxygen Therapy to Treat Diabetes Impaired Wound Healing in Rats.
PLoS ONE 9(10): e108533.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Rat CD68 antibody, clone ED1 (MCA341GA) used for the detection of macrophages in rat brain by immunohistochemistry.
Image caption:
Immunohistochemical analysis of microglial activation and neuronal loss on day 3 post-treatment with UCN. Representative photomicrographs show: (A) low power images of peri-hematomal regions as indicated by squares; (B) high power images stained with OX-42; (C) ED-1; and (D) NeuN. (E) Average cell numbers (OX 42+, ED-1+, and NeuN+) in each plane (total 6 mm2) was calculated from 54 squares in 9 planes. Values are shown as mean ± SD, calculated and analyzed by Student's t test. *p <0.05 vs. ICH + saline group.
From: Liew HK, Pang CY, Hsu CW, Wang MJ, Li TY, Peng HF, Kuo JS, Wang JY.
Systemic administration of urocortin after intracerebral hemorrhage reduces neurological deficits and neuroinflammation in rats.
J Neuroinflammation. 2012 Jan 19;9:13.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Rat CD68 antibody, clone ED1 (MCA341R) used for the identification of macrophages in rat renal tissue by immunohistochemistry on formalin fixed, paraffin embedded sections.
Representative panel of histological and immunohistochemical analysis. Microphotographies of: PAS staining, to assessment of glomerular lesions (A), Trichromic Masson staining, to evaluate interstitial expansion (B), ED-1 immunohistochemistry, to analyze macrophage infiltration in the interstitial area (C) and in the glomeruli (D), and AII immunohistochemistry, to evaluate the presence of renal cortical interstitial cells positive to AII (E).
From: Teles F, da Silva TM, da Cruz Júnior FP, Honorato VH, de Oliveira Costa H, Barbosa APF, et al. (2015)
Brazilian Red Propolis Attenuates Hypertension and Renal Damage in 5/6 Renal Ablation Model.
PLoS ONE 10(1): e0116535.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Rat CD68 antibody, clone ED1 (MCA341R) used to identify macrophages in rat peritoneal membrane by immunohistochemistry on formalin fixed, paraffin embedded tissue sections
Image caption:
Immunohistochemistry for the detection of macrophages (ED1) (200x). Only a few ED1+ cells were present in the normal peritoneal membrane (a). CKD induced a significant number of macrophages in the peritoneum (b). However, the largest number of macrophages was detected in the thickened peritoneal membrane of the PF (c) and CKD/PF groups (d). TAM (e) and rBMP7 (f) protected the peritoneal membrane against inflammatory infiltrate comprising macrophages, as shown by quantitative analysis.
From: Silva FMO, Costalonga EC, Silva C, Carreira ACO, Gomes SA, Sogayar MC, Fanelli C, Noronha IL.
Tamoxifen and bone morphogenic protein-7 modulate fibrosis and inflammation in the peritoneal fibrosis model developed in uremic rats.
Mol Med. 2019 Aug 28; 25(1): 41.
doi: 10.1186/s10020-019-0110-5.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Rat CD68 antibody, clone ED1 (MCA341R) used for the detection of macrophages in rat kidney by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
ED-1 (macrophage) staining from STNx rats. As illustrated by representative photomicrographs, in sham rats (A), only occasional macrophages were observed in the interstitium, while STNx rats (B) were associated with numerous macrophages. When compared to PPG (C) and Irb (D) mono-therapy, treatment of STNx animals with PPG+Irb (E) was associated with a further reduction in the number of macrophages. Magnification x200. Quantitative data (F) are expressed as mean ± SEM. *, P <0.05 vs sham; #, P <0.05 vs vehicle-treated;f, P <0.05 vs Irb-treated STNx rats. Animal numbers: Sham = 20, STNx = 19, STNx+PPG = 17, STNx+Irb = 19 and STNx+PPG+Irb = 16.
From: Ayoub MA, Zhang Y, Kelly RS, See HB, Johnstone EKM, et al. (2015)
Functional Interaction between Angiotensin II Receptor Type 1 and Chemokine (C-C Motif) Receptor 2 with Implications for Chronic Kidney Disease.
PLoS ONE 10(3): e0119803.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Rat CD68 antibody, clone ED1 (MCA341R) used for the demonstration of macrophages in rat kidney by immunofluorescence.
Image caption:
Immunofluorescence analysis by confocal laser scanning microscopy showed NK-1R (red) on ED1-positive macrophages (green; d). The yellow merge indicates colocalization of NK-1R with ED1-positive cells.
From: Rodionova K, Hilgers KF, Paulus EM, Tiegs G, Ott C, Schmieder R, Schiffer M, Amann K, Veelken R, Ditting T.
Neurogenic tachykinin mechanisms in experimental nephritis of rats.
Pflugers Arch. 2020 Oct 17 [Epub ahead of print].
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat CD68 antibody, clone ED1 (MCA341R) used for the demonstration of macrophages in rat kidney by immunohistochemistry on formalin fixed, paraffin embedded tissue sections..
Image caption:
Representative ED 1 staining in rats suffering from mesangioproliferaitve nephritis induced by anti Thy1.1 monoclonal antibodies (upper panel) and nephritic animals pretreated with 5 mg/kg aprepitant i.p. 12h and 1h before injection of anti-Thy1.1 and every 24h after antibody challenge (lower panel). Seven days after the inducting of Thy1.1 nephritis ED 1 staining was significantly reduced in animals pretreated with Aprepitant.
From: Rodionova K, Hilgers KF, Paulus EM, Tiegs G, Ott C, Schmieder R, Schiffer M, Amann K, Veelken R, Ditting T.
Neurogenic tachykinin mechanisms in experimental nephritis of rats.
Pflugers Arch. 2020 Oct 17 [Epub ahead of print].
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat CD68 antibody, clone ED1 (MCA341GA) used to illustrate rat microglia by immunofluorescence.
Image caption:
Cell type characterization in SDH primary cultures. Immunocytochemistry was performed to identify neurons (MAP), astrocytes (GFAP), oligodendrocytes (CNPase), and microglial cells (ED1). Percentages represent the number of immunopositive cells of each cell type referring to all investigated immunopositive cells. Scale bar represents 25 μm
From: Leisengang S, Nürnberger F, Ott D, Murgott J, Gerstberger R, Rummel C, Roth J.
Primary culture of the rat spinal dorsal horn: a tool to investigate the effects of inflammatory stimulation on the afferent somatosensory system.
Pflugers Arch. 2020 Oct 24 [Epub ahead of print].
doi: 10.1007/s00424-020-02478-y.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat CD68 antibody, clone ED1 (MCA341GA) used to illustrate rat microglia by immunofluorescence.
Image caption:
After short-term stimulation with LPS immunoreactivity of TNFα and IL-6 is predominantly induced in SDH microglial cells. a, b Using antibodies against TNFα (red) and ED1 (green, microglial marker), an increased TNFα-immunoreactivity is detectable in microglial cells after stimulation with LPS (10 μg/ml, 2 h). c, d LPS stimulation results in increased immunoreactivity for IL-6 (red) in microglial cells (green). Nuclei are stained with DAPI (blue). Scale bar represents 12.5 μm
From: Leisengang S, Nürnberger F, Ott D, Murgott J, Gerstberger R, Rummel C, Roth J.
Primary culture of the rat spinal dorsal horn: a tool to investigate the effects of inflammatory stimulation on the afferent somatosensory system.
Pflugers Arch. 2020 Oct 24 [Epub ahead of print].
doi: 10.1007/s00424-020-02478-y.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat CD68 antibody, clone ED1 (MCA341GA) used to illustrate rat microglia by immunofluorescence.
Image caption:
Nuclear translocation of transcription factors NFκB, NF-IL6, and pCREB in microglial cells of SDH primary cultures after short-term stimulation with LPS. Stimulation with LPS (10 μg/ml) for 2 h leads to an increased nuclear immunoreactivity of transcription factors NFκB (a, b red), NF-IL6 (d, e red), and pCREB (g, h red) in microglial cells (ED1, green). Measuring the mean staining intensity within the area of the nucleus (DAPI, blue) in microglial cells a significantly increased immunoreactivity can be observed due to LPS stimulation (c, f, I ***p <0.001). Columns represent the mean staining intensities ± SEM of all investigated microglial cells (n) from at least 3 distinct experiments. Scale bar represents 12.5 μm.
From: Leisengang S, Nürnberger F, Ott D, Murgott J, Gerstberger R, Rummel C, Roth J.
Primary culture of the rat spinal dorsal horn: a tool to investigate the effects of inflammatory stimulation on the afferent somatosensory system.
Pflugers Arch. 2020 Oct 24 [Epub ahead of print].
doi: 10.1007/s00424-020-02478-y.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat CD68 antibody, clone ED1 (MCA341R) used to demonstrate macrophage infiltration in intestinal tissue sections by immunofluorescence.
Image caption:
Hippocampal HIV-1 Nef expression induces intestinal inflammation.
Immunofluorescence for the macrophage marker CD68 (red) was assessed to determine the levels of macrophage infiltration between the groups while nuclei of the cell are represented (in blue). (D) Macrophages were quantified in three 40x HPFs per rat. N = 3 to 7 rats per group. Student's t-test was performed to determine statistical difference between the Nef-treated group and the GFP-treated group (control). Scale bars indicate 50 μm. Error bars indicate means ± S.E.M.
From: Rivera J, Isidro RA, Loucil-Alicea RY, Cruz ML, Appleyard CB, Isidro AA, et al. (2019)
Infusion of HIV-1 Nef-expressing astrocytes into the rat hippocampus induces enteropathy and interstitial pneumonitis and increases blood–brain-barrier permeability.
PLoS ONE 14(11): e0225760.
doi: 10.1371/journal.pone.0225760
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat CD68 antibody, clone ED1 (MCA341R) used to demonstrate macrophage infiltration in pulmonary tissue sections by immunofluorescence.
Image caption:
Nef expression in the rat hippocampus induces interstitial pneumonitis and increases macrophage infiltration and vascular permeability in the lung.
(B) Immunofluorescence for the macrophage marker CD68 in (red) while nuclei of the cells is represented in (blue) was assessed to compare macrophage infiltration between the groups. (C) Macrophages were quantified in three HPFs per rat. (D) Immunofluorescence of the tight junction protein claudin-5 in (green) while nuclei of the cells is represented in (blue) was assessed to determine tight junction alterations in the lungs. Nef-treated rats showed alterations in claudin-5 expression that were characterized by diffuse and dimmed staining compared to what was observed in the GFP-treated rats. (E) Changes in lung permeability were determined by measuring EB extravasation, which confirmed that Nef-treated rats had increased lung permeability compared to our control group. N = 4 to 6 rats per group. Scale bars indicate 25 μm. Student's t-test was performed to determine differences between the Nef-treated group and the GFP-treated group (control). Data are expressed as means ± S.E.M.
From: Rivera J, Isidro RA, Loucil-Alicea RY, Cruz ML, Appleyard CB, Isidro AA, et al. (2019)
Infusion of HIV-1 Nef-expressing astrocytes into the rat hippocampus induces enteropathy and interstitial pneumonitis and increases blood–brain-barrier permeability.
PLoS ONE 14(11): e0225760.
doi: 10.1371/journal.pone.0225760
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat CD68 antibody, clone ED1 (MCA341R) used to show macrophages in rat kidney by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Effect of CL on inflammation during TAC-induced renal injury.
Immunohistochemistry and quantitative analysis for ED-1 (OPN) in the renal cortex. Note that the number of ED-1-positive cells are increased in the TAC group and that this increase is inhibited in the TAC + CL groups. Magnification, 200×. N = 9 per group. #P <0.05 vs. VH; @P <0.05 vs. TAC
From: Luo K, Lim SW, Jin J, Jin L, Gil HW, Im DS, Hwang HS, Yang CW.
Cilastatin protects against tacrolimus-induced nephrotoxicity via anti-oxidative and anti-apoptotic properties.
BMC Nephrol. 2019 Jun 14;20(1):221.
doi: 10.1186/s12882-019-1399-6.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat CD68 antibody, clone ED-1 (MCA341R) used for the identification of macrophages in rat kee joints by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
CD68 immunostaining in the Im-B and Im-NS groups. CD68-positive cells were observed in both Im-B (a-c) and Im-NS groups (d-f). They were observed mainly in the synovial membrane of the Im-B group. After 8 weeks, there was a small difference in the number of CD68-positive cells between the two groups. F: Femur, S: Synovium, JS: Joint Space, M: Meniscus. The black arrowheads indicate CD68-positive cells. Scale bar = 100 μm
From: Sogi Y, Yabe Y, Hagiwara Y, et al.
Joint hemorrhage accelerates cartilage degeneration in a rat immobilized knee model.
BMC Musculoskelet Disord. 2020;21(1):761.
doi:10.1186/s12891-020-03795-0
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse nti Rat CD68 antibody, clone ED-1 (MCA341GA) used to identify macrophages in rat nerve tissue by immunofluorescence.
Image caption:
Ascorbic acid (AA) enhances the infiltration and phagocytosis of macrophages in the injured nerve. Immediately after sciatic nerve transection surgery, rats were intragastrically administered 400 mg/kg AA, which was followed by 200 mg/kg AA per day for 5 or 8 days. (A–P) ED1 immunofluorescence and ORO counterstaining show macrophages (white arrows) and denatured myelin (white arrows) in the injured nerves at 5 and 8 dpi. The numbers of ED1-positive cells and droplets of denatured myelin per macrophage in the AA group were significantly higher than in the saline group. (a-p) Higher magnification images of the boxes in A-P. Scale bars: 100 μm in A–P, 20 μm in a–p. (Q, R) The numbers of macrophages and droplets of denatured myelin per macrophage at 5 and 8 dpi. Data are expressed as the mean ± SEM (n = 3 per group). *P <0.05 (independent samples t-tests). dpi: Days post-injury; ED1: CD68; ORO: oil red O.
From: Li, L. et al.
Ascorbic acid accelerates Wallerian degeneration after peripheral nerve injury.
Neur Regen Res. 2021, 16:6, 1078-85.
doi: 10.4103/1673-5374.300459
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti rat CD68, clone ED1 (MCA341R) used to identify macrophages in rat choroid plexus following subarachnoid hemorrhage by immunofluorescence.
Image caption:
Representative pictures illustrating double immunostaining to detect heme oxygenase-1 (HO-1) positive ED1+ (A–C) or ED2+ (D–F) macrophages in the choroid plexus 3 days after subarachnoid hemorrhage. Merged pictures with Hoechst stained nuclei (C,F) proved that heme oxygenase-1 positive Kolmer cells are either activated (ED1+) or resident (ED2+) macrophages (arrows). Scale bars – main images 80 μm; insets 20 μm.
From: Solár P, Brázda V, Levin S, Zamani A, Jančálek R, Dubový P and Joukal M (2020)
Subarachnoid Hemorrhage Increases Level of Heme Oxygenase-1 and Biliverdin Reductase in the Choroid Plexus.
Front. Cell. Neurosci. 14:593305.
doi: 10.3389/fncel.2020.593305
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat CD68 antibody, clone ED-1 (MCA341R) used to demonstrate microglia in rat brain by immunofluorescence.
Image caption:
Microglial activation after IC transplantation or IV injection of hUC-MSCs in MCAo rats. (A) Representative confocal images of dual IHC for ED1 (green) and Iba-1 (red). DAPI (blue) counterstain. Scale bar = 50 μm.
From: Noh JE, Oh SH, Park IH, Song J.
Intracerebral Transplants of GMP-Grade Human Umbilical Cord-Derived Mesenchymal Stromal Cells Effectively Treat Subacute-Phase Ischemic Stroke in a Rodent Model.
Front Cell Neurosci. 2020 Sep 25;14:546659.
doi: 10.3389/fncel.2020.546659.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat CD68 antibody, clone ED-1 (MCA341R) used to identify macrophages in rat renal tissue by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Representative photomicrographs of the renal cortex illustrating tubulointerstitial CD68-positive cells (indicated by arrows) by immunohistochemistry. Images were captured using a morphometric program (NIS-Elements, Nikon) with a 20× objective and magnified images shown at the top were captured with a 40× objective.
From: de Araújo L, Costa-Pessoa JM, de Ponte MC, Oliveira-Souza M.
Sodium Oxalate-Induced Acute Kidney Injury Associated With Glomerular and Tubulointerstitial Damage in Rats.
Front Physiol. 2020 Aug 25;11:1076.
doi: 10.3389/fphys.2020.01076.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
FITC conjugated Mouse anti Rat CD68 antibody, clone ED-1 (MCA341F) used to evaluate CD68 expression on macrophage populations by flow cytometry.
Image caption:
Changes in the macrophage level in ST36 acupoint after MA. Macrophages labeled with CD45+CD11b+ CD68+ levels in the skin layers of ST36 acupoint at day 7 were quantitatively detected through flow cytometry. Data are expressed as the mean ± SEM; ∗P <0.05 vs. control group, #P <0.05 vs. CFA group.
From: Zhang K, Zhao X, Ding S, Liu Y, Xu Y, Yan Y, Hong S, Yang F, Wang S, Xu Z, Guo Y, Guo Y, Pang G, Wang J, Guo X, Zhao M.
Applying Complex Network and Cell-Cell Communication Network Diagram Methods to Explore the Key Cytokines and Immune Cells in Local Acupoint Involved in Acupuncture Treating Inflammatory Pain.
Evid Based Complement Alternat Med. 2020 Jul 29; 2020: 2585960.
doi: 10.1155/2020/2585960.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat Cd68 antibody, clone ED-1 (MCA341R) used to identify macrophages
Image caption:
Treatment with TG-C induces the expression of IL-10 in the OA knee joints. Synovial lavage fluid or membranes were harvested at day 4 post-treatment for immunofluorescence. Synovial membranes were double immunofluorescent stained with anti-CD68 as a macrophage marker or anti-IL-10 antibodies. The white arrow indicates co-localization of CD68 with IL-10 and is shown only in the TG-C treatment group. The scale bar indicates 50 μm.
From: Lee H, Kim H, Seo J, Choi K, Lee Y, Park K, Kim S, Mobasheri A, Choi H.
TissueGene-C promotes an anti-inflammatory micro-environment in a rat monoiodoacetate model of osteoarthritis via polarization of M2 macrophages leading to pain relief and structural improvement.
Inflammopharmacology. 2020 Oct;28(5):1237-52.
doi: 10.1007/s10787-020-00738-y.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat CD68 antibody, clone ED-1 (MCA341R) used to demonstrate macrophages in rat kidney by immunohistochemistry on formalin fixed paraffin embedded tissue sections.
Image caption:
Findings of macrophage accumulation observed by immunohistochemical staining using ED-1 antibody in renal cortex of salt-loaded stroke-prone spontaneously hypertensive rats (SHRSP)
From: Nagatani, Y.; Higashino, T.; Kinoshita, K.; Higashino, H.
Thromboxane A2 Receptor Antagonist (ONO-8809) Attenuates the Renal Disorders Caused by Salt-Overload in Stroke-Prone Spontaneously Hypertensive Rats.
Preprints 2021, 2021010319.
doi: 10.20944/preprints202101.0319.v1.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat CD68 antibody, clone ED1 (MCA341R) used to stain microglia in rat brain by immunofluorescence.
Image caption:
EC2319 treatment effects in rats during the chronic phase of fDTH-EAE.
a Low- and high-power magnification photomicrographs of double immunofluorescence staining for FR-β and CD68 in fDTH-EAE rat brain cryosections. Scale bars, 50 μm (high power) and 2 mm (low power).
From: Elo P, Li XG, Liljenbäck H, Gardberg M, Moisio O, Miner M, Virta J, Saraste A, Srinivasarao M, Pugh M, Low PS, Knuuti J, Jalkanen S, Airas L, Lu YJ, Roivainen A.
Efficacy and tolerability of folate-aminopterin therapy in a rat focal model of multiple sclerosis.
J Neuroinflammation. 2021 Jan 20;18(1):30.
doi: 10.1186/s12974-021-02073-7.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat CD68 antibody, clone ED1 (MCA341R) used to label macrophages in formalin fixed rat peritoneal membrane sections bt immunohistochemistry.
Image caption:
Illustrative microphotographs of peritoneal samples from the different groups submitted to immunohistochemistry for macrophage (a)
From: Costalonga EC, Fanelli C, Garnica MR, Noronha IL.
Adipose-Derived Mesenchymal Stem Cells Modulate Fibrosis and Inflammation in the Peritoneal Fibrosis Model Developed in Uremic Rats.
Stem Cells Int. 2020 May 20;2020:3768718.
doi: 10.1155/2020/3768718.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat CD68 antibody, clone ED-1 (MCA341G) used to label macrophages in vitro by immunofluorescence.
Image caption:
Microglia morphology (white arrows) at a magnification of 630 × . ED1 staining allowed the classification of microglia as resting ramified (a), intermediate (b) and activated rounded phagocytic (c) phenotype under hyperammonemia.
From: Ismail FS, Faustmann TJ, Corvace F, Tsvetanova A, Moinfar Z, Faustmann PM.
Ammonia induced microglia activation was associated with limited effects on connexin 43 and aquaporin 4 expression in an astrocyte-microglia co-culture model.
BMC Neurosci. 2021 Mar 25;22(1):21.
doi: 10.1186/s12868-021-00628-1.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat CD68 antibody, clone ED1 (MCA341GA) used for the assessment of CD68 expression on formaldehyde fixed rat brain sections by immunofluorescence.
Image caption:
Phenotyping of TTR positive cells.
TTR immunoreactivity (AF488, green) was not found in NeuN positive neurons, GFAP positive astrocytes or CD68 positive microglia/macrophages, 14 days after PT. CD68 shown in red (Cy3). TTR is expressed in the epithelial cells of CP, used as a positive control. Scale bars 50 μm. CP–Choroid plexus;IC–Infarct core; nuclei; PI–peri-infarct area; TTR–Transthyretin.
From: Talhada D, Gonçalves I, Reis Santos C, Ruscher K (2019)
Transthyretin expression in the postischemic brain.
PLoS ONE 14(9): e0221555.
https://doi.org/10.1371/journal.pone.0221555
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Rat CD68 antibody, clone ED1 (MCA341GA) used for visualizing macrophages in rat corneal sections by immunofluorescence.
Image caption:
Localization of CD68 positive cells and VEGF in Ch and 7KCh implants.
Rat eyes were implanted with 20% Ch and 7KCh wafers and cryosections prepared 14 days post implantation. The sections were labeled with AlexaFluor 488 (green) for anti-CD68 or anti-VEGF and the nuclei stained with DAPI (blue). A. Ch implant, anti-CD68. B. 7KCh implant, anti-CD68. C. Ch implant, anti-VEGF. D. 7KCh implant, anti-VEGF.
From: Amaral J, Lee JW, Chou J, Campos MM, Rodríguez IR (2013)
7-Ketocholesterol Induces Inflammation and Angiogenesis In Vivo: A Novel Rat Model.
PLoS ONE 8(2): e56099.
doi: 10.1371/journal.pone.0056099
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat CD68 antibody, clone ED1 (MCA341R) used to label macrophages in rat kidney by immunohistochemistry on formalin fixed, paraffin embedded tissue sections
Image caption:
Anti-inflammatory effect of EC2319. (A) Immunohistochemistry stained for ED1+ monocyte/macrophages, infiltrates of kidney sections of rats with anti-GBM GN.
From: Garcia GE, Lu YJ, Truong LD, Roncal-Jiménez CA, Miyazaki M, Miyazaki-Anzai S, Cara-Fuentes G, Andres-Hernando A, Lanaspa M, Johnson RJ, Leamon CP.
A Novel Treatment for Glomerular Disease: Targeting the Activated Macrophage Folate Receptor with a Trojan Horse Therapy in Rats.
Cells. 2021 Aug 17;10(8):2113.
doi: 10.3390/cells10082113.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat CD68 antibody, clone ED1 (MCA341R) used to assess purity of macrophages from different sources by flow cytometry on Leucoperm permeablized cells.
Image caption:
Adult rat macrophage populations.
Macrophages were isolated from adult male Dark Agouti rats. (B) Permeabilized macrophages were analysed by flow cytometry to assess purity via CD68 expression. Quadrants were set with isotype controls. Dot plots are representative of cells isolated from three rats. Rat alveolar (AM), peritoneal (PM), BM-derived (BMDM) and monocyte-derived (MDM) macrophages
From: Pridans C, Irvine KM, Davis GM, Lefevre L, Bush SJ, Hume DA.
Transcriptomic Analysis of Rat Macrophages.
Front Immunol. 2021 Feb 1;11:594594.
doi: 10.3389/fimmu.2020.594594.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat CD68 antibody, clone ED1 (MCA341R) used to stain macrophages in rat prostate by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Immunostaining of intraprostatic rat tumours. Sections from AT1 tumours growing alone, AT1 tumours growing next to MLL tumours, MLL tumours growing alone, and MLL tumour growing next to AT1 stained for Factor VIII, and CD68. AT1 tumours growing adjacent to MLL showed increased Factor VIII and CD68 staining at the tumour border compared to AT1 tumours growing alone.
From: Halin Bergström S, Rudolfsson S, Lundholm M, Josefsson A, Wikström P, Bergh A.
High-grade tumours promote growth of other less-malignant tumours in the same prostate.
J Pathol. 2021 Apr;253(4):396-403.
doi: 10.1002/path.5604.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat cd68 antibody, clone ED1 (MCA341R) used to label macrophages in rat brain by immunofluorescence on cryostat sections.
Image caption:
Immunofluorescent staining of tissue reactions around the microelectrode cluster array. (A) Visualization of NeuN (neurons; red), ED1 (activated microglia; green) and DAPI (all nuclei; blue) staining in the implantation area. Dashed line encircles the approximate electrode cluster area. Rectangular box delineates the area enlarged in B. (B) High magnification image showing NeuN and ED1 staining near a single microelectrode in an area with high neuronal density (DAPI was omitted from this panel for enhanced clarity of the microglial and neuronal density). (C) GFAP (astrocytes) and (D) RECA (blood vessel)-staining of the same area in a section 80μm below the section shown in A and B. All scale bars are 50μm.
From: Mohammed M, Ivica N, Bjartmarz H, Thorbergsson PT, Pettersson LME, Thelin J, Schouenborg J.
Microelectrode clusters enable therapeutic deep brain stimulation without noticeable side-effects in a rodent model of Parkinson's disease.
J Neurosci Methods. 2022 Jan 1;365:109399.
doi: 10.1016/j.jneumeth.2021.109399.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat CD68 antibody, clone ED1 (MCA341R) used to identify macrophages and osteoclasts in rat knee joints by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Cell types identified in MSU/LPS-injected joints at day 70 (day 14 after the fifth MSU/LPS injection). (a) Main cell types identified in MSU/LPS-injected joints were polymorphonuclear neutrophils, mast cells and macrophages. Scale bars represent 20μm. (b) Immunohistochemistry of CD68 confirmed the presence of macrophages and osteoclasts. Scale bars represent 50μm.
From: Accart N, Dawson J, Obrecht M, Lambert C, Flueckiger M, Kreider J, Hatakeyama S, Richards PJ, Beckmann N.
Degenerative joint disease induced by repeated intra-articular injections of monosodium urate crystals in rats as investigated by translational imaging.
Sci Rep. 2022 Jan 7;12(1):157.
doi: 10.1038/s41598-021-04125-7.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse nti rat CD68 antibody. clone ED1 (MCA341R) used to identify macrophages in rat bladder by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Representative immunohistochemical co-staining and line graph for CD68 (brown), CD86 (M1-like macrophages; red) and CD206 (M2-like macrophages; red) in rat bladder tissues at necropsy (n = 5 animals per group). Data are presented as mean values ± S.D. (IHC scores). Statistical analyses done using two-tailed Student’s t-test (*P <0.05).
From: Singh AK, Praharaj M, Lombardo KA, Yoshida T, Matoso A, Baras AS, Zhao L, Srikrishna G, Huang J, Prasad P, Powell JD, Kates M, McConkey D, Pardoll DM, Bishai WR, Bivalacqua TJ.
Re-engineered BCG overexpressing cyclic di-AMP augments trained immunity and exhibits improved efficacy against bladder cancer.
Nat Commun. 2022 Feb 15;13(1):878.
doi: 10.1038/s41467-022-28509-z.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat CD68 antibody, clone ED1 (MCA341R) used to identify macrophage lineage cells in rat tetinal wholemounts by immunofluorescence.
Image caption:
Representative images of retinal explant cryosections cocultured with 1.104 MSCs at DEV 0 and DEV 7 immunolabeled with CD68 (red) (B) and DiO-labeled MSCs (green) at ×200 magnification (scale bar = 100μm). D Quantification of CD68 immunoreactivity (expressed as Raw Integrated Density). n = 4–6 animals/group. Ordinary one-way ANOVA was performed. F RT-qPCR analysis of CD68 expression in retinal explants at DEV 0 and DEV 7. mRNA levels are presented after normalization with the housekeeping gene Rps18. n = 12–13 animals/group. Unpaired t-test was performed for unpaired comparisons. ****P <0.0001
From: Reboussin É, Buffault J, Brignole-Baudouin F, Réaux-Le Goazigo A, Riancho L, Olmiere C, Sahel JA, Mélik Parsadaniantz S, Baudouin C.
Evaluation of neuroprotective and immunomodulatory properties of mesenchymal stem cells in an ex vivo retinal explant model.
J Neuroinflammation. 2022 Mar 2;19(1):63.
doi: 10.1186/s12974-022-02418-w.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat CD68 antibody, clone ED1 (MCA341R) used to identify macrophages in rat renal tissue by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Representative micrographs of renal sections double staining for in situ superoxide formation (indicated by NBT, blue formazan particles, black arrowheads) and CD68 (monocyte and macrophage marker) immunostaining. Left panel: A–D: Non-A (Atorvastatin) experimental groups; Right panel: E–H: A-treated experimental groups. (A) Control group: only a few orange-red stained CD68-positive cells with NBT score of 0+. (B) Hyperlipidemia (HL) group: 3–5 CD68-positive cells in the interstitium, with NBT score of 0+. (C) Rats treated with 5% hydroxyproline (HP): CD68-positive cells located in dilated renal tubule cells and widened interstitium, with NBT of 3+-4+. (D) HL+HP group: many CD68-positive cells located in the interstitium with NBT score of 2+-3+ (E) A group: 1–5 CD68-positive cells in the glomerulus, with NBT score of 0+. (F) A+HL group: 1–5 CD68-positive cells, with NBT score of 0+. (G) A+HP group: many dark-stained CD68-positive cells in the interstitium, with NBT score of 0+-1+. (H) A+HL+HP group: 15–30 CD68-positive cells, with NBT score of 1+. All images above were demonstrated with original magnification 400×. (I) Relationship between changes in NBT scoring and CD68-positive cells in immunostained kidney samples; positive correlation is shown, with r = 0.935, p <0.001 in the non-A treated groups and r = 0.641, p <0.001 in the A-treated group.
From: Liu, C.J. Tsai, Y.S. Huang, H.S.
Atorvastatin Decreases Renal Calcium Oxalate Stone Deposits by Enhancing Renal Osteopontin Expression in Hyperoxaluric Stone-Forming Rats Fed a High-Fat Diet.
Int J Mol Sci. 2022, 23, 3048.
doi: 10.3390/ijms23063048
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Mouse anti Rat CD68 antibody, clone ED1 (MCA341R) used to label macrophages in rat kidney by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Infiltrating macrophages after kidney transplantation in vehicle and Rapa treated rats.
(A) ED1 and (B) ED2 positive macrophages in the cortex calculated as percentage of positive interstitial cells by immunohistochemistry. Representative photomicrographs are shown. n = 3. (C) Comparison of ED1 and ED2 positive infiltrating cells on day 7 in vehicle and Rapa treated rats. Cortex and outer medulla were analyzed together. n = 6–8. (D) Semi-quantitative real-time PCR for mRNA transcripts of inducible NO synthase (iNOS) in whole tissue extracts from kidney allografts. n = 4–6. *P<0.05, **P<0.01, ***P<0.001.
From: Hoff U, Markmann D, Thurn-Valassina D, Nieminen-Kelhä M, Erlangga Z, Schmitz J, et al.. (2022)
The mTOR inhibitor Rapamycin protects from premature cellular senescence early after experimental kidney transplantation.
PLoS ONE 17(4): e0266319.
doi: 10.1371/journal.pone.0266319
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat CD68 antibody, clone ED1 (MCA341R) used to detect macrophages in rat liver by immunohistochemistry on cryosections.
Image caption:
Immunohistochemical detection of MPO (marker for neutrophils) and ED1 (marker for macrophages) in rat livers after TO injection administered intramuscularly into the right and left hindlimbs of rats at a concentration of 5 mL/kg. Control livers, which received only saline (top) and TO-treated liver (bottom). Few MPO+ cells were detected in the control rat liver (A), which were not increased after TO treatment (B). ED1-positive cells were detected in the sinusoidal region of the control livers (C), and their number was also not increased after TO administration in rat liver (D). The presented image is representative of three experiments. Counted MPO (E) and ED-1 (F)-positive cells in the liver. Results represent the mean ± SEM values of three animals (n = 3) and six slides.
From: Malik IA, Ramadori G.
Interleukin-6-Production Is Responsible for Induction of Hepatic Synthesis of Several Chemokines as Acute-Phase Mediators in Two Animal Models: Possible Significance for Interpretation of Laboratory Changes in Severely Ill Patients.
Biology (Basel). 2022 Mar 18;11(3):470.
doi: 10.3390/biology11030470.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat CD68 antibody, clone ED1 (MCA341R) used to label macrophages and monocytes in rat lymph nodes by immunohistochemistry on cryostat sections.
Image caption:
Subcapsular sinus of the rat popliteal lymph node stained for the presence of macrophages and monocytes (CD68+, red). (A) The node of the control animal receiving 0.9% NaCl (days 1–7, node evaluation on day 8). (B) The node after initial massive S. epidermidis infection (days 1–7) with early node evaluation (day 8). (C) The node after secondary. S. epidermidis infection (day 28, node evaluation on day 29). Magnification 200×.
From: Cąkała-Jakimowicz, M.; Puzianowska-Kuznicka, M.
Towards Understanding the Lymph Node Response to Skin Infection with Saprophytic Staphylococcus epidermidis.
Biomedicines 2022, 10, 1021.
doi: 10.3390/biomedicines10051021
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat CD68 antibody, clone ED1 (MCA341R) used to label macrophages and monocytes in rat lymph nodes by immunohistochemistry on cryostat sections.
Image caption:
The medulla of the rat popliteal lymph node in initial massive S. epidermidis infection (days 1–7) with early node’s evaluation (day 8). The node stained for: (A) Dendritic cells (OX62+, red). (B) Macrophages and monocytes (CD68+, red). (C) Stem cells, thymocytes and immature B cells (CD90+, red). (D) T helper lymphocytes and monocytes (CD4+, red). (E) Activated antigen-presenting cells (MHC class II+, red). Magnification 1000×.
From: Cąkała-Jakimowicz, M.; Puzianowska-Kuznicka, M.
Towards Understanding the Lymph Node Response to Skin Infection with Saprophytic Staphylococcus epidermidis.
Biomedicines 2022, 10, 1021.
doi: 10.3390/biomedicines10051021
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat CD68 antibody, clone ED1 (MCA341R) used to stain macrophages in mouose aortic tissue by immunohistochemistry on formalin fixed, paraffin embedded sections.
Image caption:
Histopathology and immunohistochemical staining of thoracic aorta lesions.
Macrophage (anti-CD68) staining. GF: germ free, SPF: specific pathogen free.
From: Chuang H-L, Chiu C-C, Lo C, Hsu C-C, Liu J-Y, Hung S-W, et al. (2022)
Circulating gut microbiota-related metabolites influence endothelium plaque lesion formation in ApoE knockout rats.
PLoS ONE 17(5): e0264934.
doi: 10.1371/journal.pone.0264934
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat CD68 antibody, clone ED1 (MCA341R) used to label macrophages in rat lung by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Immunohistochemistry in lung tissue: immunostaining by CD68 and CD3 (M-T).
In N we identified in pinkish red the immunostaining for CD68 in the pulmonary interstitium of the NP Wistar group, showing a slight number of cells positive for CD68 and when compared with its respective control (M). In contrast, we observed an increase in cells immunostained for CD68 in lung parenchyma of NP Lewis group (P) when compared to its control group (O). In R, note in brown the immunostaining for CD3 lymphocyte in the lung parenchyma NP Wistar group, when compared to the control group (Q) and in T, we identified CD68-positive cells in the thickening of pulmonary interstitium and control (S).
From: Silva EKVB, Bomfim CG, Barbosa AP, Noda P, Noronha IL, Fernandes BHV, et al. (2022)
Immunization with SARS-CoV-2 Nucleocapsid protein triggers a pulmonary immune response in rats.
PLoS ONE 17(5): e0268434.
doi: 10.1371/journal.pone.0268434
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat CD68 antibody, clone ED1 (MCA341R) used to label macrophages in rat nervous tissue by immunofluorescence on cryosections and immunohistochemistry on paraffin sections.
Image caption:
Bupivacaine alleviated microglia immunoreactivity in CCI rats. Rats were intrathecally injected with the indicated concentrations of bupivacaine (0 and 0.5%, diluted in saline) for 30 min prior to being subjected to sham and CCI operation. Seven days later, frozen sections of ipsilateral spinal cords (L4–L6) (A) and paraffin sections of ipsilateral sciatic nerves (B) were prepared and subjected to immunohistochemistry with antibodies recognizing CD68. Scale bar: 50 μm. Representative photomicrographs show immunoreactivity visualized with FITC fluorescence ((A), counterstained with Hoechst 33342) and diaminobenzidine ((B), counterstained with hematoxylin), respectively. Relative intensity of immunoreactivity was measured and depicted under photomicrographs (%). * p <0.05 vs. sham/saline group and # p <0.05 vs. CCI/saline group, n = 3.
From: Wu CC, Chang CY, Tzeng CY, Huang JH, Hung CJ, Chen WY, Liao SL, Kuan YH, Chen CJ.
Preventive Intrathecal Injection of Bupivacaine Alleviated Microglia Activation and Neuropathic Pain in a Rat Model of Chronic Constriction Injury.
Int J Mol Sci. 2022 Jun 28;23(13):7197.
doi: 10.3390/ijms23137197.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat CD68 antibody, clone ED1 MCA341R) used to label macrophages in rat renal tissue by immunofluorescence.
Image caption:
Renal macrophage infiltration. (a) Immunohistochemistry staining of the macrophage marker CD68 in renal cortex (upper panel) and medulla (lower panel) of the kidney from rats in sham, ischemia/reperfusion (I/R), I/R with capsaicin pretreatment (I/R+CAP), sham with liposome-encapsulated clodronate (LC) treatment (sham+LC), I/R+LC, and CAP+I/R+LC groups. Arrows indicate CD68-positive macrophages. Scale bar, 50μm. Magnification: 40x. Quantification of macrophages in renal cortex (b) and medulla (c). Values are mean ± SE (n = 5). *P<0.05 vs. sham, †P<0.05 vs. I/R, #P<0.05 vs. the corresponding LC-free group.
From: Yu SQ, Ma S, Wang DH.
Ablation of TRPV1-positive nerves exacerbates salt-induced hypertension and tissue injury in rats after renal ischemia-reperfusion via infiltration of macrophages.
Clin Exp Hypertens. 2021 Apr 3;43(3):254-262.
doi: 10.1080/10641963.2020.1860078.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat CD68 antibody, clone ED1 (MCA341R) used to label macrophages in rat brain by immunofluorescence.
Image caption:
Givinostat has no effect on the polarization of microglia after neonatal hypoxia-ischemia. Sections from ipsilateral (hypoxic-ischemic) hemispheres 7 and 14 days after HI were stained for anti-ED1 antibody-labeled reactive phagocytic microglia (red) and for interleukin-1β (IL-1β), a marker specific for the proinflammatory M1 phenotype (green). Nuclei were labeled with DAPI (blue). Representative confocal photomicrographs of brain sections show double-labeled cells (yellow) 7 days (A) and 14 days (B) after HI. Enlargements present areas marked in rectangles. Number of ED1/IL-1β-positive cells quantified in the brain cortex area (0.36 mm2) 7 days (C) and 14 days (D) after HI. The values are means ± SD from five animals per group. One-way ANOVA and Tukey’s test did not indicate any significant differences between the investigated groups.
From: Pawelec, P. Sypecka, J. Zalewska, T. Ziemka-Nalecz, M.
Analysis of Givinostat/ITF2357 Treatment in a Rat Model of Neonatal Hypoxic-Ischemic Brain Damage.
Int J Mol Sci. 2022, 23, 8287.
doi: 10.3390/ijms23158287
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat CD68 antibody, clone ED1 (MCA341R) used to label macrophages in rat spinal cord by immunofluorescence.
Image caption:
Activation of macrophages after SCI. (a–c) Confocal microscopy images show examples of macrophages in the SCI lesion center at 4 dpo. All cells are IR for Iba-1 (a, green) and most also for CD68 (b, red), shown in combination with nuclear staining (b,c, Hoechst 33342, blue). (d–f) CD68 IR macrophages in the spinal cord after laminectomy (d, sham), SCI (e), and SCI 2 × 300 mg/kg TUDCA treatment (f). Same magnifications are used in (a–c) and in (d–f) (scale bars in (c,f)).
From: Wu S, García-Rama C, Romero-Ramírez L, de Munter JPJM, Wolters EC, Kramer BW, Mey J.
Tauroursodeoxycholic Acid Reduces Neuroinflammation but Does Not Support Long Term Functional Recovery of Rats with Spinal Cord Injury.
Biomedicines. 2022 Jun 25;10(7):1501.
doi: 10.3390/biomedicines10071501.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat CD68 antibody, clone ED1 (MCA341R) used to label macrophages in rat brain by immunohistochemistry on formalin fixed cryosections.
Image caption:
Progressive macrophage/microglia activation in the internal capsule following endothelin-1 (ET-1) striatal injection. The inflammatory response is minimal in the control group (A). At 1PLD it is possible to notice a small number of ED1+ cells (B), followed by a significant increase at 3PLD (C), with a decrease at 7PLD (D), as confirmed by quantitative analysis (E) (* p < 0.05; ** p < 0.01; *** p < 0.001, ANOVA, Tukey post hoc test). Values expressed as mean ± SEM. Arrows in B, C and D point to ED1+ cells. Drawings at the left side of the figure show the anatomical localization of the ET-1 injection in the striatum. Ctx: cortex; CPu: caudate putamen (striatum); Di: diencephalon; Hip: hippocampus; ic: internal capsule. Scale bar: 50 μm.
From: Freire, M.A.M.; Lima, R.R.; Bittencourt, L.O.; Guimaraes, J.S.; Falcao, D.; Gomes-Leal, W.
Astrocytosis, Inflammation, Axonal Damage and Myelin Impairment in the Internal Capsule following Striatal Ischemic Injury.
Cells 2023, 12, 457.
doi:10.3390/cells12030457
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti rat CD68 antibody, clone ED1 (MCA341R) used to identify macrophages in renal tissue by immunohistochemistry on formalin fixed, paraffin embedded sections.
Image caption:
Immunolocalization of ED1-positive cells (macrophages) in the outer renal medulla of the SAL (A), SAL + calcitriol (B), CP (C) and CP + calcitriol (D) groups (bar represents 50 μm). The number of ED1-positive cells in all experimental groups’ outer renal medulla (E). Values are given as the mean ± SEM.
From: IOliveira BM, de Almeida LF, Deluque AL, Souza CS, Maciel ALD, Francescato HDC, Costa RS, Giovanini C, de Paula FJA, Coimbra TM.
Calcitriol Reduces the Inflammation, Endothelial Damage and Oxidative Stress in AKI Caused by Cisplatin.
Int J Mol Sci. 2022 Dec 14;23(24):15877.
doi: 10.3390/ijms232415877.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
FITC conjugated Mouse anti Rat CD68 antibody, clone ED1 (MCA341F) used to evaluate CD68 expression on rat bronchiolar lavage cells by flow cytometry.
Image caption:
IBTK inhibition regulated macrophage polarization in MCT-induced PAH. Expression of CD68+ (macrophages), CD68+CD86+ (M1), and CD68+CD163+ (M2) in bronchoalveolar lavage was evaluated by flow cytometry analysis. (b–d) Representative images of flow cytometry analysis. (e–g) Percentages of CD68+ macrophages, CD68+CD86+ macrophages, and CD68+CD163+ macrophages in bronchoalveolar lavage of each treatment group (n = 6–8). Data are presented as mean ± SEM. ∗P < 0.05 vs. control; #P < 0.05 vs. MCT.
From: Yu M, Wu X, Peng L, Yang M, Zhou H, Xu J, Wang J, Wang H, Xie W, Kong H.
Inhibition of Bruton's Tyrosine Kinase Alleviates Monocrotaline-Induced Pulmonary Arterial Hypertension by Modulating Macrophage Polarization.
Oxid Med Cell Longev. 2022 Aug 29;2022:6526036.
doi: 10.1155/2022/6526036.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat CD68 antibody, clone ED1 (MCA341R) used to stain macrophages in rat kidney by immunohistochemistry on methacarn fixed, paraffin embedded tissue sections.
Image caption:
Immunohistochemical analysis for CD68+ cells expression in rat kidney tissue.
(A) Bar graph of CD68+ cells expression values. (B) Representative photomicrographs of immunostaining for CD68+ cells in the renal cortex from a Sham, Li, Gly and Gly+Li rat (x400). Values are means ± SEM. ap<0.001 vs. Sham; dp<0.001 vs. Li; hp<0.01 vs. Gly.
Li, lithium; Gly, glycerol; Gly+Li, glycerol + lithium.
From: Shimizu MHM, Volpini RA, de Bragança AC, Nascimento MM, Bernardo DRD, Seguro AC, et al. (2023)
Administration of a single dose of lithium ameliorates rhabdomyolysis-associated acute kidney injury in rats.
PLoS ONE 18(2): e0281679.
doi: 10.1371/journal.pone.0281679.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
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- CD68
Antibody Quality and Performance Guarantee
| Mouse anti rat CD68 antibody, clone ED1 recognizes the rat ED1 antigen, a heavily glycosylated protein of ~90 -110 kDa, also known as rat CD68 (Dijkstra et al. 1985). The ED1 antigen is expressed on most macrophages populations, as well as on monocytes and is considered as a pan-macrophage marker in the rat (Damoiseaux et al. 1994). ED1 is expressed predominantly on the lysosomal membrane and lightly on the cell surface (Dijkstra et al. 1985). The expression of ED1 antigen being predominantly cytoplasmic (Dijkstra et al. 1985), flow cytometry results are improved by the use of a membrane permeabilization procedure, such as Leucoperm, prior to staining. |
Our CD68 (ED1) Antibody has been referenced in >580 publications* *Based on June 2020 data from CiteAb's antibody search engine. |
- Target Species
- Rat
- Species Cross-Reactivity
-
Target Species Cross Reactivity Bovine Horse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- MCA341GA: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- MCA341R: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
- Buffer Solution
- Phosphate buffered saline.
- Preservative Stabilisers
- Pack Size: 0.1 mg
0.09% Sodium Azide Pack Size: 0.25 mg0.09% sodium azide. - Carrier Free
- Yes
- Immunogen
- Rat spleen cells
- Approx. Protein Concentrations
- Pack Size: 0.1 mgIgG concentration 0.5 mg/mlPack Size: 0.25 mgIgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0-Ag14 mouse myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry 1 |  |
1/50 | 1/100 |
| Immunofluorescence | |
||
| Immunohistology - Frozen | |
||
| Immunohistology - Paraffin 2 | |
1/100 | |
| Immunoprecipitation | |
||
| Western Blotting | |
- 1 Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.
- 2 This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA1209 | F | 0.1 mg |
|
Log in | ||
| List Price | Your Price | ||||||
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Log in | ||||||
| Description | Mouse IgG1 Negative Control | ||||||
Source Reference
-
Dijkstra, C.D. et al. (1985) The heterogeneity of mononuclear phagocytes in lymphoid organs: distinct macrophage subpopulations in the rat recognized by monoclonal antibodies ED1, ED2 and ED3.
Immunology. 54 (3): 589-99.
References for CD68 antibody
-
Damoiseaux, J.G. et al. (1994) Rat macrophage lysosomal membrane antigen recognized by monoclonal antibody ED1.
Immunology. 83 (1): 140-7. -
Bauer, J. et al. (1994) Phagocytic activity of macrophages and microglial cells during the course of acute and chronic relapsing experimental autoimmune encephalomyelitis.
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Thom, S.R. et al. (2004) Delayed neuropathology after carbon monoxide poisoning is immune-mediated.
Proc Natl Acad Sci U S A. 101 (37): 13660-5. -
Wu, L. et al. (2004) Dietary approach to attenuate oxidative stress, hypertension, and inflammation in the cardiovascular system.
Proc Natl Acad Sci U S A. 101 (18): 7094-9. -
Pellkofer, H. et al. (2004) Modelling paraneoplastic CNS disease: T-cells specific for the onconeuronal antigen PNMA1 mediate autoimmune encephalomyelitis in the rat.
Brain. 127 (Pt 8): 1822-30. -
Korn, T. et al. (2004) Modulation of effector cell functions in experimental autoimmune encephalomyelitis by leflunomide--mechanisms independent of pyrimidine depletion.
J Leukoc Biol. 76 (5): 950-60.
View The Latest Product References
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- Synonyms
- ED1
- RRID
- AB_2291300
- UniProt
- Q4FZY1
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