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CD68 antibody | ED1

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Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F * IF IP P * WB 0.1 mg loader
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Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F * IF IP P * WB 0.25 mg loader
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Mouse anti rat CD68 antibody, clone ED1 recognizes the rat ED1 antigen, a heavily glycosylated protein of ~90 -110 kDa, also known as rat CD68 (Dijkstra et al. 1985).

The ED1 antigen is expressed on most macrophages populations, as well as on monocytes and is considered as a pan-macrophage marker in the rat (Damoiseaux et al. 1994). ED1 is expressed predominantly on the lysosomal membrane and lightly on the cell surface (Dijkstra et al. 1985).

The expression of ED1 antigen being predominantly cytoplasmic (Dijkstra et al. 1985), flow cytometry results are improved by the use of a membrane permeabilization procedure, such as Leucoperm, prior to staining.
CiteAb logo - trusted, tested, published

Our CD68 (ED1) Antibody has been referenced in >580 publications*

*Based on June 2020 data from CiteAb's antibody search engine.

Target Species
Species Cross-Reactivity
Target SpeciesCross Reactivity
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
MCA341GA: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
MCA341R: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
Pack Size: 0.1 mg
0.09%Sodium Azide
Pack Size: 0.25 mg
0.09% sodium azide.
Carrier Free
Rat spleen cells
Approx. Protein Concentrations
Pack Size: 0.1 mg
IgG concentration 0.5 mg/ml
Pack Size: 0.25 mg
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0-Ag14 mouse myeloma cell line.
For research purposes only
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1 1/50 1/100
Immunohistology - Frozen
Immunohistology - Paraffin 2 1/100
Western Blotting
  1. 1 Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.
  2. 2 This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

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Source Reference

  1. Dijkstra, C.D. et al. (1985) The heterogeneity of mononuclear phagocytes in lymphoid organs: distinct macrophage subpopulations in the rat recognized by monoclonal antibodies ED1, ED2 and ED3.
    Immunology. 54 (3): 589-99.

References for CD68 antibody

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