Mouse anti Rat CD4 (domain 2) antibody, clone OX-35
recognizes the rat CD4 cell surface antigen, a ~55kDa glycoprotein expressed by helper T cells and weakly by monocytes.
Mouse anti Rat CD4 (Domain 2) antibody, clone OX-35 recognizes a different epitope on the CD4 molecule to Mouse anti Rat CD4 antibody, clone W3/25
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- Carrier Free
- MLR generated rat T helper lymphocytes.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
integral to membrane
protein kinase binding
T cell selection
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of CD4 antibody
|Immunohistology - Frozen
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
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Secondary Antibodies Available
Negative Isotype Controls Available
Application Based External Images
Product Specific References
References for CD4 antibody
Wang, C.C. et al. (1996) Immunohistochemical study of amoeboid microglial cells in fetal rat brain.
J Anat. 189 ( Pt 3): 567-74.
Jefferies, W.A. et al. (1985) Authentic T helper CD4 (W3/25) antigen on rat peritoneal macrophages.
J Exp Med. 162 (1): 117-27.
Camelo, S. et al. (2004) The distribution of antigen in lymphoid tissues following its injection into the anterior chamber of the rat eye.
J Immunol. 172: 5388-95.
Elflein, K. et al. (2003) Rapid recovery from T lymphopenia by CD28 superagonist therapy.
Blood. 102: 1764-70.
Scherr, M. et al. (2002) Efficient gene transfer into the CNS by lentiviral vectors purified by anion exchange chromatography.
Gene Ther. 9: 1708-14.
Cho, K.S. et al. (2010) Mechanism analysis of long-term graft survival by monocarboxylate transporter-1 inhibition.
Transplantation. 90: 1299-306.
Chang, C.J. et al (2004) The immunization site of cytokine-secreting tumor cell vaccines influences the trafficking of tumor-specific T lymphocytes and antitumor efficacy against regional tumors.
J Immunol. 173: 6025-32.
Basiri, M. and Doucette, R. (2010) Sensorimotor cortex aspiration: a model for studying Wallerian degeneration-induced glial reactivity along the entire length of a single CNS axonal pathway.
Brain Res Bull. 81: 43-52.
Esquifino, A.I. et al. (2007) Immune response after experimental allergic encephalomyelitis in rats subjected to calorie restriction.
J Neuroinflammation. 4:6.
Zhao, S. et al. (2007) Extensive FDG uptake and its modification with corticosteroid in a granuloma rat model: an experimental study for differentiating granuloma from tumors.
Eur J Nucl Med Mol Imaging 34: 2096-105.
Yan, Y. et al. (2003) Pathogenesis of autoimmunity after xenogeneic thymus transplantation.
J Immunol. 170: 5936-46.
Li, Q. et al. (2019) Dendritic cell-targeted CD40 DNA vaccine suppresses Th17 and ameliorates progression of experimental autoimmune glomerulonephritis
Journal of Leukocyte Biology. 27 Feb [Epub ahead of print].
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