CD4 antibody | OX-35






Mouse anti Rat CD4 (Domain 2) antibody, clone OX-35 recognizes a different epitope on the CD4 molecule to Mouse anti Rat CD4 antibody, clone W3/25.
Product Details
- Target Species
- Rat
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- MLR generated rat T helper lymphocytes.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P05540
- Entrez Gene
- Cd4
- GO Terms
- GO:0007155 cell adhesion
- GO:0016021 integral to membrane
- GO:0006955 immune response
- GO:0019901 protein kinase binding
- GO:0045058 T cell selection
- Regulatory
- For research purposes only
Applications of CD4 antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | 1/10 | 1/50 | |
Immunofluorescence | |||
Immunohistology - Frozen | |||
Immunoprecipitation |
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Secondary Antibodies Available
Negative Isotype Controls Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Mouse IgG2a Negative Control | MCA1210 | F | 100 Tests |
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Product Specific References
References for CD4 antibody
-
Wang, C.C. et al. (1996) Immunohistochemical study of amoeboid microglial cells in fetal rat brain.
J Anat. 189 ( Pt 3): 567-74. -
Jefferies, W.A. et al. (1985) Authentic T helper CD4 (W3/25) antigen on rat peritoneal macrophages.
J Exp Med. 162 (1): 117-27. -
Camelo, S. et al. (2004) The distribution of antigen in lymphoid tissues following its injection into the anterior chamber of the rat eye.
J Immunol. 172: 5388-95. -
Elflein, K. et al. (2003) Rapid recovery from T lymphopenia by CD28 superagonist therapy.
Blood. 102: 1764-70. -
Scherr, M. et al. (2002) Efficient gene transfer into the CNS by lentiviral vectors purified by anion exchange chromatography.
Gene Ther. 9: 1708-14. -
Cho, K.S. et al. (2010) Mechanism analysis of long-term graft survival by monocarboxylate transporter-1 inhibition.
Transplantation. 90: 1299-306. -
Chang, C.J. et al (2004) The immunization site of cytokine-secreting tumor cell vaccines influences the trafficking of tumor-specific T lymphocytes and antitumor efficacy against regional tumors.
J Immunol. 173: 6025-32. -
Basiri, M. and Doucette, R. (2010) Sensorimotor cortex aspiration: a model for studying Wallerian degeneration-induced glial reactivity along the entire length of a single CNS axonal pathway.
Brain Res Bull. 81: 43-52. -
Esquifino, A.I. et al. (2007) Immune response after experimental allergic encephalomyelitis in rats subjected to calorie restriction.
J Neuroinflammation. 4:6. -
Zhao, S. et al. (2007) Extensive FDG uptake and its modification with corticosteroid in a granuloma rat model: an experimental study for differentiating granuloma from tumors.
Eur J Nucl Med Mol Imaging 34: 2096-105. -
Yan, Y. et al. (2003) Pathogenesis of autoimmunity after xenogeneic thymus transplantation.
J Immunol. 170: 5936-46. -
Li, Q. et al. (2019) Dendritic cell-targeted CD40 DNA vaccine suppresses Th17 and ameliorates progression of experimental autoimmune glomerulonephritis
Journal of Leukocyte Biology. 27 Feb [Epub ahead of print].
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