CD4 antibody | OX-35
Mouse anti Rat CD4 antibody, clone OX-35 (MCA153R) used to detect CD4 expressing cells in rat tongue by immunohistochemistry on cryostat sections.
Image caption:
Mapping of immune cells in the ventral surface of tongues from rats, dogs, minipigs and monkeys.
Mucosal tissues from animal species were processed for immunohistology analysis, as described in Methods. Slides were stained with the following specific antibodies: anti-MHC-II and anti-CD4 to detect and quantify positive cells in tissue sections (magnification x200). Representative photomicrographs of mucosal tissue sections are shown.
From: Thirion-Delalande C, Gervais F, Fisch C, Cuiné J, Baron-Bodo V, Moingeon P, et al. (2017)
Comparative analysis of the oral mucosae from rodents and non-rodents: Application to the nonclinical evaluation of sublingual immunotherapy products.
PLoS ONE 12(9): e0183398. DOI: 10.1371/journal.pone.0183398
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Rat CD4 antibody, clone OX-35 (MCA153R) used to label T helper lymphocytes in meningeal sections of rat brain by immunofluorescence. Section also stained for cytotoxic T cells using Mouse anti Rat CD8 antibody, clone OX-8 (MCA48R).
Image caption:
Injection of cytokine expressing vectors into the subarachnoid space of rats leads to meningeal inflammation.
Immunofluorescent staining showed the extensive infiltration of CD4+ and CD8+ T cells in the meninges within the sagittal sulcus and overlying the cortex of cytokine vector injected MOG animals.
From: James RE, Schalks R, Browne E, Eleftheriadou I, Munoz CP, Mazarakis ND, Reynolds R.
Persistent elevation of intrathecal pro-inflammatory cytokines leads to multiple sclerosis-like cortical demyelination and neurodegeneration.
Acta Neuropathol Commun. 2020 May 12;8(1):66.
doi: 10.1186/s40478-020-00938-1.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat CD4 antibody, clone OX-35 (MCA153R) used to label T helper lymphocytes in meningeal sections of rat brain by immunofluorescence. Section also stained for cytotoxic T cells using Mouse anti Rat CD8 antibody, clone OX-8 (MCA48R).
Image caption:
Injection of cytokine expressing vectors into the subarachnoid space of rats leads to meningeal inflammation.
Immunofluorescent staining showed the extensive infiltration of CD4+ and CD8+ T cells in the meninges within the sagittal sulcus and overlying the cortex of cytokine vector injected MOG animals.
From: James RE, Schalks R, Browne E, Eleftheriadou I, Munoz CP, Mazarakis ND, Reynolds R.
Persistent elevation of intrathecal pro-inflammatory cytokines leads to multiple sclerosis-like cortical demyelination and neurodegeneration.
Acta Neuropathol Commun. 2020 May 12;8(1):66.
doi: 10.1186/s40478-020-00938-1.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat CD4 (domain 2) antibody, clone OX-35 recognizes the rat CD4 cell surface antigen, a ~55kDa glycoprotein expressed by helper T cells and weakly by monocytes.
Mouse anti Rat CD4 (Domain 2) antibody, clone OX-35 recognizes a different epitope on the CD4 molecule to Mouse anti Rat CD4 antibody, clone W3/25.
Mouse anti Rat CD4 (Domain 2) antibody, clone OX-35 recognizes a different epitope on the CD4 molecule to Mouse anti Rat CD4 antibody, clone W3/25.
Product Details
- Target Species
- Rat
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- MLR generated rat T helper lymphocytes.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P05540
- Entrez Gene
- Cd4
- GO Terms
- GO:0007155 cell adhesion
- GO:0016021 integral to membrane
- GO:0006955 immune response
- GO:0019901 protein kinase binding
- GO:0045058 T cell selection
- Regulatory
- For research purposes only
Applications of CD4 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | 1/10 | 1/50 | |
| Immunofluorescence | |||
| Immunohistology - Frozen | |||
| Immunoprecipitation |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG2a Negative Control | MCA1210 | F | 100 Tests |
|
Product Specific References
References for CD4 antibody
-
Wang, C.C. et al. (1996) Immunohistochemical study of amoeboid microglial cells in fetal rat brain.
J Anat. 189 ( Pt 3): 567-74. -
Jefferies, W.A. et al. (1985) Authentic T helper CD4 (W3/25) antigen on rat peritoneal macrophages.
J Exp Med. 162 (1): 117-27. -
Camelo, S. et al. (2004) The distribution of antigen in lymphoid tissues following its injection into the anterior chamber of the rat eye.
J Immunol. 172: 5388-95. -
Elflein, K. et al. (2003) Rapid recovery from T lymphopenia by CD28 superagonist therapy.
Blood. 102: 1764-70. -
Scherr, M. et al. (2002) Efficient gene transfer into the CNS by lentiviral vectors purified by anion exchange chromatography.
Gene Ther. 9: 1708-14. -
Cho, K.S. et al. (2010) Mechanism analysis of long-term graft survival by monocarboxylate transporter-1 inhibition.
Transplantation. 90: 1299-306. -
Chang, C.J. et al (2004) The immunization site of cytokine-secreting tumor cell vaccines influences the trafficking of tumor-specific T lymphocytes and antitumor efficacy against regional tumors.
J Immunol. 173: 6025-32. -
Basiri, M. and Doucette, R. (2010) Sensorimotor cortex aspiration: a model for studying Wallerian degeneration-induced glial reactivity along the entire length of a single CNS axonal pathway.
Brain Res Bull. 81: 43-52. -
Esquifino, A.I. et al. (2007) Immune response after experimental allergic encephalomyelitis in rats subjected to calorie restriction.
J Neuroinflammation. 4:6. -
Zhao, S. et al. (2007) Extensive FDG uptake and its modification with corticosteroid in a granuloma rat model: an experimental study for differentiating granuloma from tumors.
Eur J Nucl Med Mol Imaging 34: 2096-105. -
Yan, Y. et al. (2003) Pathogenesis of autoimmunity after xenogeneic thymus transplantation.
J Immunol. 170: 5936-46. -
Li, Q. et al. (2019) Dendritic cell-targeted CD40 DNA vaccine suppresses Th17 and ameliorates progression of experimental autoimmune glomerulonephritis
Journal of Leukocyte Biology. 27 Feb [Epub ahead of print]. -
Thirion-Delalande, C. et al. (2017) Comparative analysis of the oral mucosae from rodents and non-rodents: Application to the nonclinical evaluation of sublingual immunotherapy products.
PLoS One. 12 (9): e0183398. -
James, R.E. et al. (2020) Persistent elevation of intrathecal pro-inflammatory cytokines leads to multiple sclerosis-like cortical demyelination and neurodegeneration.
Acta Neuropathol Commun. 8 (1): 66. -
Dabrowska, S. et al. (2021) Neuroinflammation evoked by brain injury in a rat model of lacunar infarct.
Exp Neurol. 336: 113531.
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