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CD4 antibody | OX-35

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Anti Rat CD4 (Domain 2) Antibody, clone OX-35 thumbnail image 1 Anti Rat CD4 (Domain 2) Antibody, clone OX-35 thumbnail image 2 Anti Rat CD4 (Domain 2) Antibody, clone OX-35 thumbnail image 3 Anti Rat CD4 (Domain 2) Antibody, clone OX-35 thumbnail image 4 Anti Rat CD4 (Domain 2) Antibody, clone OX-35 thumbnail image 5 Anti Rat CD4 (Domain 2) Antibody, clone OX-35 thumbnail image 6 Anti Rat CD4 (Domain 2) Antibody, clone OX-35 thumbnail image 7 Anti Rat CD4 (Domain 2) Antibody, clone OX-35 thumbnail image 8
Anti Rat CD4 (Domain 2) Antibody, clone OX-35 gallery image 1
Figure A. RPE conjugated Mouse anti Rat CD44 (MCA643PE) and Alexa Fluor 647 conjugated Mouse IgG2a isotype control (MCA1210A647). Figure B. RPE conjugated Mouse anti Rat CD44 (MCA643PE) and Alexa Fluor 647 conjugated Mouse anti Rat CD4 (MCA153A647). All experiments performed on red cell lysed rat blood gated on lymphoid cells in the presence of 10% rat serum. Data acquired on the ZE5™ Cell Analyzer.
Anti Rat CD4 (Domain 2) Antibody, clone OX-35 gallery image 2
Figure A. RPE conjugated Mouse anti Rat CD44 (MCA643PE) and Alexa Fluor 647 conjugated Mouse IgG2a isotype control (MCA1210A647). Figure B. RPE conjugated Mouse anti Rat CD44 (MCA643PE) and RPE conjugated Mouse anti Rat CD4 (MCA153A647). All experiments performed on red cell lysed rat blood gated on mononuclear cells.
Anti Rat CD4 (Domain 2) Antibody, clone OX-35 gallery image 3
Figure A. Alexa Fluor 647 conjugated Mouse anti Rat CD4 (MCA153A647) and RPE conjugated Mouse IgG2a isotype control (MCA1210PE). Figure B. Alexa Fluor 647 conjugated Mouse anti Rat CD4 (MCA153A647) and RPE conjugated Mouse anti Rat CD44 (MCA643PE). All experiments performed on red cell lysed rat blood gated on mononuclear cells.
Anti Rat CD4 (Domain 2) Antibody, clone OX-35 gallery image 4
Immunoperoxidase staining of rat lymph node cryosection using Mouse anti Rat CD4 antibody (MCA153) followed by horseradish peroxidase conjugated Goat anti Mouse IgG2a (STAR133P) as a detection reagent. Low power
Anti Rat CD4 (Domain 2) Antibody, clone OX-35 gallery image 5
Immunoperoxidase staining of rat lymph node cryosection using Mouse anti Rat CD4 antibody (MCA153) followed by horseradish peroxidase conjugated Goat anti Mouse IgG2a (STAR133P) as a detection reagent. Low power
Anti Rat CD4 (Domain 2) Antibody, clone OX-35 gallery image 6
Immunofluorescence staining of rat lymph node cryosection using Mouse anti Rat CD4 antibody (MCA153), red in A and Mouse anti Rat CD8 (MCA48), green in B. C is merged image with nuclear counterstaining with DAPI in blue. Low power
Anti Rat CD4 (Domain 2) Antibody, clone OX-35 gallery image 7
Immunofluorescence staining of rat lymph node cryosection using Mouse anti Rat CD4 antibody (MCA153), red in A and Mouse anti Rat CD8 (MCA48), green in B. C is merged image with nuclear counterstaining with DAPI in blue. High power
Anti Rat CD4 (Domain 2) Antibody, clone OX-35 gallery image 8
Immunoperoxidase staining of rat lymph node cryosection using Mouse anti Rat CD4 antibody (MCA153) followed by horseradish peroxidase conjugated Goat anti Mouse IgG2a (STAR133P) as a detection reagent. High power

Mouse anti Rat CD4 (Domain 2):Alexa Fluor® 647

Mouse anti Rat CD4 (Domain 2)

Product Type
Monoclonal Antibody
Clone
OX-35
Isotype
IgG2a
Product Code Applications Pack Size List Price Quantity
MCA153A647
Datasheet
F
MSDS
100 Tests/1ml loader

MCA153GA
Datasheet
C F IF IP
MSDS
0.1 mg loader

MCA153R
Datasheet
C F IF
MSDS
0.25 mg loader

Mouse anti Rat CD4 (Domain 2) antibody, clone OX-35 recognizes the rat CD4 cell surface antigen, a ~55kDa glycoprotein expressed by helper T cells and weakly by monocytes.

Mouse anti Rat CD4 (Domain 2) antibody, clone OX-35 recognizes a different epitope on the CD4 molecule to Mouse anti Rat CD4 antibody, clone W3/25.

Product Details

Target Species
Rat
Product Form
Purified IgG conjugated to Alexa Fluor® 647 - liquid
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
MLR generated rat T helper lymphocytes.
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
18 months from date of despatch.
Guarantee
18 months from date of despatch.

More Information

UniProt
P05540 Related reagents
Entrez Gene
Cd4 Related reagents
GO Terms
GO:0007155 cell adhesion
GO:0016021 integral to membrane
GO:0006955 immune response
GO:0019901 protein kinase binding
GO:0045058 T cell selection
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Regulatory
For research purposes only

Applications of CD4 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Flow Cytometry 1/10 1/50
Immunofluorescence
Immunohistology - Frozen
Immunoprecipitation
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Human anti Mouse IgG2a:FITC HCA037F F 0.1 mg loader
Human anti Mouse IgG2a:HRP HCA037P E 0.1 mg loader
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG2a Negative Control:Alexa Fluor® 647 MCA1210A647 F 100 Tests/1ml loader
Mouse IgG2a Negative Control MCA1210 F 100 Tests loader

Application Based External Images

Flow Cytometry

Immunofluorescence

Product Specific References

References for CD4 antibody

  1. Wang, C.C. et al. (1996) Immunohistochemical study of amoeboid microglial cells in fetal rat brain.
    J Anat. 189 ( Pt 3): 567-74.
  2. Jefferies, W.A. et al. (1985) Authentic T helper CD4 (W3/25) antigen on rat peritoneal macrophages.
    J Exp Med. 162 (1): 117-27.
  3. Camelo, S. et al. (2004) The distribution of antigen in lymphoid tissues following its injection into the anterior chamber of the rat eye.
    J Immunol. 172: 5388-95.
  4. Elflein, K. et al. (2003) Rapid recovery from T lymphopenia by CD28 superagonist therapy.
    Blood. 102: 1764-70.
  5. Scherr, M. et al. (2002) Efficient gene transfer into the CNS by lentiviral vectors purified by anion exchange chromatography.
    Gene Ther. 9: 1708-14.
  6. Cho, K.S. et al. (2010) Mechanism analysis of long-term graft survival by monocarboxylate transporter-1 inhibition.
    Transplantation. 90: 1299-306.
  7. Chang, C.J. et al (2004) The immunization site of cytokine-secreting tumor cell vaccines influences the trafficking of tumor-specific T lymphocytes and antitumor efficacy against regional tumors.
    J Immunol. 173: 6025-32.
  8. Basiri, M. and Doucette, R. (2010) Sensorimotor cortex aspiration: a model for studying Wallerian degeneration-induced glial reactivity along the entire length of a single CNS axonal pathway.
    Brain Res Bull. 81: 43-52.
  9. Esquifino, A.I. et al. (2007) Immune response after experimental allergic encephalomyelitis in rats subjected to calorie restriction.
    J Neuroinflammation. 4:6.
  10. Zhao, S. et al. (2007) Extensive FDG uptake and its modification with corticosteroid in a granuloma rat model: an experimental study for differentiating granuloma from tumors.
    Eur J Nucl Med Mol Imaging 34: 2096-105.
  11. Yan, Y. et al. (2003) Pathogenesis of autoimmunity after xenogeneic thymus transplantation.
    J Immunol. 170: 5936-46.
  12. Li, Q. et al. (2019) Dendritic cell-targeted CD40 DNA vaccine suppresses Th17 and ameliorates progression of experimental autoimmune glomerulonephritis
    Journal of Leukocyte Biology. 27 Feb [Epub ahead of print].

CD4 resources

CD4 articles

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