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CD4 antibody | OX-35

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Anti Rat CD4 (Domain 2) Antibody, clone OX-35 thumbnail image 1 Anti Rat CD4 (Domain 2) Antibody, clone OX-35 thumbnail image 2 Anti Rat CD4 (Domain 2) Antibody, clone OX-35 thumbnail image 3 Anti Rat CD4 (Domain 2) Antibody, clone OX-35 thumbnail image 4 Anti Rat CD4 (Domain 2) Antibody, clone OX-35 thumbnail image 5 Anti Rat CD4 (Domain 2) Antibody, clone OX-35 thumbnail image 6 Anti Rat CD4 (Domain 2) Antibody, clone OX-35 thumbnail image 7 Anti Rat CD4 (Domain 2) Antibody, clone OX-35 thumbnail image 8
Anti Rat CD4 (Domain 2) Antibody, clone OX-35 gallery image 1
Immunoperoxidase staining of rat lymph node cryosection using Mouse anti Rat CD4 antibody (MCA153) followed by horseradish peroxidase conjugated Goat anti Mouse IgG2a (STAR133P) as a detection reagent. Low power
Anti Rat CD4 (Domain 2) Antibody, clone OX-35 gallery image 2
Immunoperoxidase staining of rat lymph node cryosection using Mouse anti Rat CD4 antibody (MCA153) followed by horseradish peroxidase conjugated Goat anti Mouse IgG2a (STAR133P) as a detection reagent. Low power
Anti Rat CD4 (Domain 2) Antibody, clone OX-35 gallery image 3
Immunofluorescence staining of rat lymph node cryosection using Mouse anti Rat CD4 antibody (MCA153), red in A and Mouse anti Rat CD8 (MCA48), green in B. C is merged image with nuclear counterstaining with DAPI in blue. Low power
Anti Rat CD4 (Domain 2) Antibody, clone OX-35 gallery image 4
Immunofluorescence staining of rat lymph node cryosection using Mouse anti Rat CD4 antibody (MCA153), red in A and Mouse anti Rat CD8 (MCA48), green in B. C is merged image with nuclear counterstaining with DAPI in blue. High power
Anti Rat CD4 (Domain 2) Antibody, clone OX-35 gallery image 5
Immunoperoxidase staining of rat lymph node cryosection using Mouse anti Rat CD4 antibody (MCA153) followed by horseradish peroxidase conjugated Goat anti Mouse IgG2a (STAR133P) as a detection reagent. High power
Anti Rat CD4 (Domain 2) Antibody, clone OX-35 gallery image 6
Published customer image:
Mouse anti Rat CD4 antibody, clone OX-35 (MCA153R) used to detect CD4 expressing cells in rat tongue by immunohistochemistry on cryostat sections.
Image caption:
Mapping of immune cells in the ventral surface of tongues from rats, dogs, minipigs and monkeys.
Mucosal tissues from animal species were processed for immunohistology analysis, as described in Methods. Slides were stained with the following specific antibodies: anti-MHC-II and anti-CD4 to detect and quantify positive cells in tissue sections (magnification x200). Representative photomicrographs of mucosal tissue sections are shown.

From: Thirion-Delalande C, Gervais F, Fisch C, Cuiné J, Baron-Bodo V, Moingeon P, et al. (2017)
Comparative analysis of the oral mucosae from rodents and non-rodents: Application to the nonclinical evaluation of sublingual immunotherapy products.
PLoS ONE 12(9): e0183398. DOI: 10.1371/journal.pone.0183398
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Rat CD4 (Domain 2) Antibody, clone OX-35 gallery image 7
Published customer image:
Mouse anti Rat CD4 antibody, clone OX-35 (MCA153R) used to label T helper lymphocytes in meningeal sections of rat brain by immunofluorescence. Section also stained for cytotoxic T cells using Mouse anti Rat CD8 antibody, clone OX-8 (MCA48R).
Image caption:
Injection of cytokine expressing vectors into the subarachnoid space of rats leads to meningeal inflammation.
Immunofluorescent staining showed the extensive infiltration of CD4+ and CD8+ T cells in the meninges within the sagittal sulcus and overlying the cortex of cytokine vector injected MOG animals.

From: James RE, Schalks R, Browne E, Eleftheriadou I, Munoz CP, Mazarakis ND, Reynolds R.
Persistent elevation of intrathecal pro-inflammatory cytokines leads to multiple sclerosis-like cortical demyelination and neurodegeneration.
Acta Neuropathol Commun. 2020 May 12;8(1):66.
doi: 10.1186/s40478-020-00938-1.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Anti Rat CD4 (Domain 2) Antibody, clone OX-35 gallery image 8
Published customer image:
Mouse anti Rat CD4 antibody, clone OX-35 (MCA153R) used to label T helper lymphocytes in meningeal sections of rat brain by immunofluorescence. Section also stained for cytotoxic T cells using Mouse anti Rat CD8 antibody, clone OX-8 (MCA48R).
Image caption:
Injection of cytokine expressing vectors into the subarachnoid space of rats leads to meningeal inflammation.
Immunofluorescent staining showed the extensive infiltration of CD4+ and CD8+ T cells in the meninges within the sagittal sulcus and overlying the cortex of cytokine vector injected MOG animals.

From: James RE, Schalks R, Browne E, Eleftheriadou I, Munoz CP, Mazarakis ND, Reynolds R.
Persistent elevation of intrathecal pro-inflammatory cytokines leads to multiple sclerosis-like cortical demyelination and neurodegeneration.
Acta Neuropathol Commun. 2020 May 12;8(1):66.
doi: 10.1186/s40478-020-00938-1.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

Mouse anti Rat CD4 (Domain 2)

Product Type
Monoclonal Antibody
Clone
OX-35
Isotype
IgG2a
Product Code Applications Pack Size List Price Quantity
MCA153GA
Datasheet
C F IF IP
SDS
0.1 mg loader

MCA153R
Datasheet
C F IF
SDS
0.25 mg loader

Mouse anti Rat CD4 (domain 2) antibody, clone OX-35 recognizes the rat CD4 cell surface antigen, a ~55kDa glycoprotein expressed by helper T cells and weakly by monocytes.

Mouse anti Rat CD4 (Domain 2) antibody, clone OX-35 recognizes a different epitope on the CD4 molecule to Mouse anti Rat CD4 antibody, clone W3/25.

Product Details

Target Species
Rat
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
MLR generated rat T helper lymphocytes.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P05540
Entrez Gene
Cd4
GO Terms
GO:0007155 cell adhesion
GO:0016021 integral to membrane
GO:0006955 immune response
GO:0019901 protein kinase binding
GO:0045058 T cell selection
Regulatory
For research purposes only

Applications of CD4 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/10 1/50
Immunofluorescence
Immunohistology - Frozen
Immunoprecipitation
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
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Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Human anti Mouse IgG2a:HRP HCA037P E 0.1 mg loader
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG2a Negative Control MCA1210 F 100 Tests loader

Application Based External Images

Flow Cytometry

Product Specific References

References for CD4 antibody

  1. Wang, C.C. et al. (1996) Immunohistochemical study of amoeboid microglial cells in fetal rat brain.
    J Anat. 189 ( Pt 3): 567-74.
  2. Jefferies, W.A. et al. (1985) Authentic T helper CD4 (W3/25) antigen on rat peritoneal macrophages.
    J Exp Med. 162 (1): 117-27.
  3. Camelo, S. et al. (2004) The distribution of antigen in lymphoid tissues following its injection into the anterior chamber of the rat eye.
    J Immunol. 172: 5388-95.
  4. Elflein, K. et al. (2003) Rapid recovery from T lymphopenia by CD28 superagonist therapy.
    Blood. 102: 1764-70.
  5. Scherr, M. et al. (2002) Efficient gene transfer into the CNS by lentiviral vectors purified by anion exchange chromatography.
    Gene Ther. 9: 1708-14.
  6. Cho, K.S. et al. (2010) Mechanism analysis of long-term graft survival by monocarboxylate transporter-1 inhibition.
    Transplantation. 90: 1299-306.
  7. Chang, C.J. et al (2004) The immunization site of cytokine-secreting tumor cell vaccines influences the trafficking of tumor-specific T lymphocytes and antitumor efficacy against regional tumors.
    J Immunol. 173: 6025-32.
  8. Basiri, M. and Doucette, R. (2010) Sensorimotor cortex aspiration: a model for studying Wallerian degeneration-induced glial reactivity along the entire length of a single CNS axonal pathway.
    Brain Res Bull. 81: 43-52.
  9. Esquifino, A.I. et al. (2007) Immune response after experimental allergic encephalomyelitis in rats subjected to calorie restriction.
    J Neuroinflammation. 4:6.
  10. Zhao, S. et al. (2007) Extensive FDG uptake and its modification with corticosteroid in a granuloma rat model: an experimental study for differentiating granuloma from tumors.
    Eur J Nucl Med Mol Imaging 34: 2096-105.
  11. Yan, Y. et al. (2003) Pathogenesis of autoimmunity after xenogeneic thymus transplantation.
    J Immunol. 170: 5936-46.
  12. Li, Q. et al. (2019) Dendritic cell-targeted CD40 DNA vaccine suppresses Th17 and ameliorates progression of experimental autoimmune glomerulonephritis
    Journal of Leukocyte Biology. 27 Feb [Epub ahead of print].
  13. Thirion-Delalande, C. et al. (2017) Comparative analysis of the oral mucosae from rodents and non-rodents: Application to the nonclinical evaluation of sublingual immunotherapy products.
    PLoS One. 12 (9): e0183398.
  14. James, R.E. et al. (2020) Persistent elevation of intrathecal pro-inflammatory cytokines leads to multiple sclerosis-like cortical demyelination and neurodegeneration.
    Acta Neuropathol Commun. 8 (1): 66.

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