CD169 antibody | ED3











Product Details
- Target Species
- Rat
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- Rat Spleen cell homogenate
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0-Ag 14 mouse myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of CD169 antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | 1/500 | ||
Immunofluorescence | |||
Immunohistology - Frozen | 1/50 | 1/250 | |
Immunoprecipitation |
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Secondary Antibodies Available
Negative Isotype Controls Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Mouse IgG2a Negative Control | MCA1210 | F | 100 Tests |
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Product Specific References
References for CD169 antibody
-
Dijkstra, C.D. et al. (1985) The heterogeneity of mononuclear phagocytes in lymphoid organs: distinct macrophage subpopulations in the rat recognized by monoclonal antibodies ED1, ED2 and ED3.
Immunology. 54 (3): 589-99. -
van Rees, E.P. et al. (1985) The postnatal development of cell populations in the rat popliteal lymph node. An immunohistochemical study.
Cell Tissue Res. 242 (2): 391-8. -
van den Berg, T.K. et al. (1992) Sialoadhesin on macrophages: its identification as a lymphocyte adhesion molecule.
J Exp Med. 176 (3): 647-55. -
Fujita, E. et al. (2010) Statin Attenuates Experimental Anti-Glomerular Basement Membrane Glomerulonephritis Together with the Augmentation of Alternatively Activated Macrophages.
Am J Pathol. 177: 1143-54. -
Savikko, J. et al. (2011) Early short-term imatinib treatment is sufficient to prevent the development of chronic allograft nephropathy.
Nephrol Dial Transplant. 26 (9): 3026-32. -
Camelo, S. et al. (2006) Antigen from the anterior chamber of the eye travels in a soluble form to secondary lymphoid organs via lymphatic and vascular routes.
Invest Ophthalmol Vis Sci. 47: 1039-46. -
Camelo, S. et al. (2004) The distribution of antigen in lymphoid tissues following its injection into the anterior chamber of the rat eye.
J Immunol. 172: 5388-95. -
Savikko, J. et al. (2011) Early short-term imatinib treatment is sufficient to prevent the development of chronic allograft nephropathy.
Nephrol Dial Transplant. 26: 3026-32. -
Richards, P.J. et al. (1999) Liposomal clodronate eliminates synovial macrophages, reduces inflammation and ameliorates joint destruction in antigen-induced arthritis.
Rheumatology (Oxford). 38: 818-25. -
Lobato-Pascual, A. et al. (2013) Rat macrophage C-type lectin is an activating receptor expressed by phagocytic cells.
PLoS One. 8: e57406. -
Allen, A.R. et al. (1999) Endothelial expression of VCAM-1 in experimental crescentic nephritis and effect of antibodies to very late antigen-4 or VCAM-1 on glomerular injury.
J Immunol. 162: 5519-27. -
Homo-Delarche, F. et al. (2006) Islet inflammation and fibrosis in a spontaneous model of type 2 diabetes, the GK rat.
Diabetes. 55: 1625-33. -
Ikezumi, Y. et al. (2000) An anti-CD5 monoclonal antibody ameliorates proteinuria and glomerular lesions in rat mesangioproliferative glomerulonephritis.
Kidney Int. 58: 100-14. -
Nakamura, K. et al. (2002) Lymph node macrophages, but not spleen macrophages, express high levels of unmasked sialoadhesin: implication for the adhesive properties of macrophages in vivo.
Glycobiology. 12: 209-16. -
Rintala, J.M. et al. (2016) Epidermal growth factor receptor inhibition with erlotinib ameliorates anti-Thy 1.1-induced experimental glomerulonephritis.
J Nephrol. 29 (3): 359-65. -
Rintala, J.M. et al. (2016) Oral Platelet-Derived Growth Factor and Vascular Endothelial Growth Factor Inhibitor Sunitinib Prevents Chronic Allograft Injury in Experimental Kidney Transplantation Model.
Transplantation. 100 (1): 103-10. -
Palin, N.K. et al. (2015) Intensive perioperative simvastatin treatment protects from chronic kidney allograft injury.
Am J Nephrol. 41 (4-5): 383-91. -
Rintala, J.M. et al. (2014) Epidermal growth factor inhibition, a novel pathway to prevent chronic allograft injury.
Transplantation. 98 (8): 821-7. -
Gonçalves J et al. (2016) Potential of mannan or dextrin nanogels as vaccine carrier/adjuvant systems
Journal of Bioactive and Compatible Polymers. Mar 14 [Epub ahead of print] -
Gonçalves J et al. (2017) Extended-access methamphetamine self-administration elicits neuroinflammatory response along with blood-brain barrier breakdown.
Brain Behav Immun. 62: 306-317. -
Palin, N.K. et al. (2017) Activin inhibition limits early innate immune response in rat kidney allografts-a pilot study.
Transpl Int. 30 (1): 96-107.
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