CD169 antibody | ED3
Mouse anti Rat CD169 antibody, clone ED3 (MCA343GA) used to identify CD169 expressing cells in rat spleen by innunohistochemistry on cryosections.
Image caption:
Immunostaining of rat spleen. Serial-cut frozen sections were stained with mAbs towards A, rat MCL B, rat CD169 C, rat MHC class II D, human MHC class I (negative control) and visualized with peroxidase conjugated secondary antibody and DAB substrate. RP: red pulp. PALS: periarteriolar lymphoid sheath. FOLL: follicle. MZ: marginal zone.
From: Lobato-Pascual A, Saether PC, Dahle MK, Gaustad P, Dissen E, et al. (2013)
Rat Macrophage C-Type Lectin Is an Activating Receptor Expressed by Phagocytic Cells.
PLoS ONE 8(2): e57406.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License. Published customer image:
Immunostaining of rat spleen. Serial-cut frozen sections were stained with mAbs towards A, rat MCL B, rat CD169 C, rat MHC class II D, human MHC class I (negative control) and visualized with peroxidase conjugated secondary antibody and DAB substrate. RP: red pulp. PALS: periarteriolar lymphoid sheath. FOLL: follicle. MZ: marginal zone.
From: Lobato-Pascual A, Saether PC, Dahle MK, Gaustad P, Dissen E, et al. (2013) Rat Macrophage C-Type Lectin Is an Activating Receptor Expressed by Phagocytic Cells. PLoS ONE 8(2): e57406.
Systemically administered liposomes home primarily to splenic marginal zone and red pulp macrophages. (a,b) Healthy rats were injected with 5 mg/kg DiI-labeled PCLs and PSLs. Splenic cryosections were stained with CD169 (a, marginal metallophilic and marginal zone macrophages) and CD68 (b, red pulp macrophages). One representative experiment is shown (20× magnification).
From: Bogie JF, Jorissen W, Mailleux J, Nijland PG, Zelcer N, Vanmierlo T, Van Horssen J, Stinissen P, Hellings N, Hendriks JJ. Myelin alters the inflammatory phenotype of macrophages by activating PPARs. Acta Neuropathol Commun. 2013 Aug 2;1(1):43.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti rat CD169 antibody, clone ED3 recognises the rat CD169 cell surface antigen, a ~185 kDa molecule expressed by macrophages, predominately confined to lymphoid organs only. Monocytes and granulocytes are negative. No other cell types are positive. The most conspicious property of ED3 is it stains marginal zone macrophages and marginal metallophils in the spleen very strongly. Furthermore, macrophages in (auto-immune) diseased tissues express the ED3 antigen. In healthy tissue no expression occurs. CD169 is a receptor for glycoconjugates containing sialic acid.
Product Details
- Target Species
- Rat
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- Rat Spleen cell homogenate
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0-Ag 14 mouse myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 18 months from date of despatch.
More Information
- Regulatory
- For research purposes only
Applications of CD169 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | 1/500 | ||
| Immunofluorescence | |||
| Immunohistology - Frozen | 1/50 | 1/250 | |
| Immunoprecipitation |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG2a Negative Control | MCA1210 | F | 100 Tests |
|
Product Specific References
References for CD169 antibody
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Dijkstra, C.D. et al. (1985) The heterogeneity of mononuclear phagocytes in lymphoid organs: distinct macrophage subpopulations in the rat recognized by monoclonal antibodies ED1, ED2 and ED3.
Immunology. 54 (3): 589-99. -
van Rees, E.P. et al. (1985) The postnatal development of cell populations in the rat popliteal lymph node. An immunohistochemical study.
Cell Tissue Res. 242 (2): 391-8. -
van den Berg, T.K. et al. (1992) Sialoadhesin on macrophages: its identification as a lymphocyte adhesion molecule.
J Exp Med. 176 (3): 647-55. -
Fujita, E. et al. (2010) Statin Attenuates Experimental Anti-Glomerular Basement Membrane Glomerulonephritis Together with the Augmentation of Alternatively Activated Macrophages.
Am J Pathol. 177: 1143-54. -
Savikko, J. et al. (2011) Early short-term imatinib treatment is sufficient to prevent the development of chronic allograft nephropathy.
Nephrol Dial Transplant. 26 (9): 3026-32. -
Camelo, S. et al. (2006) Antigen from the anterior chamber of the eye travels in a soluble form to secondary lymphoid organs via lymphatic and vascular routes.
Invest Ophthalmol Vis Sci. 47: 1039-46. -
Camelo, S. et al. (2004) The distribution of antigen in lymphoid tissues following its injection into the anterior chamber of the rat eye.
J Immunol. 172: 5388-95. -
Savikko, J. et al. (2011) Early short-term imatinib treatment is sufficient to prevent the development of chronic allograft nephropathy.
Nephrol Dial Transplant. 26: 3026-32. -
Richards, P.J. et al. (1999) Liposomal clodronate eliminates synovial macrophages, reduces inflammation and ameliorates joint destruction in antigen-induced arthritis.
Rheumatology (Oxford). 38: 818-25. -
Lobato-Pascual, A. et al. (2013) Rat macrophage C-type lectin is an activating receptor expressed by phagocytic cells.
PLoS One. 8: e57406. -
Allen, A.R. et al. (1999) Endothelial expression of VCAM-1 in experimental crescentic nephritis and effect of antibodies to very late antigen-4 or VCAM-1 on glomerular injury.
J Immunol. 162: 5519-27. -
Homo-Delarche, F. et al. (2006) Islet inflammation and fibrosis in a spontaneous model of type 2 diabetes, the GK rat.
Diabetes. 55: 1625-33. -
Ikezumi, Y. et al. (2000) An anti-CD5 monoclonal antibody ameliorates proteinuria and glomerular lesions in rat mesangioproliferative glomerulonephritis.
Kidney Int. 58: 100-14. -
Nakamura, K. et al. (2002) Lymph node macrophages, but not spleen macrophages, express high levels of unmasked sialoadhesin: implication for the adhesive properties of macrophages in vivo.
Glycobiology. 12: 209-16. -
Rintala, J.M. et al. (2016) Epidermal growth factor receptor inhibition with erlotinib ameliorates anti-Thy 1.1-induced experimental glomerulonephritis.
J Nephrol. 29 (3): 359-65. -
Rintala, J.M. et al. (2016) Oral Platelet-Derived Growth Factor and Vascular Endothelial Growth Factor Inhibitor Sunitinib Prevents Chronic Allograft Injury in Experimental Kidney Transplantation Model.
Transplantation. 100 (1): 103-10. -
Palin, N.K. et al. (2015) Intensive perioperative simvastatin treatment protects from chronic kidney allograft injury.
Am J Nephrol. 41 (4-5): 383-91. -
Rintala, J.M. et al. (2014) Epidermal growth factor inhibition, a novel pathway to prevent chronic allograft injury.
Transplantation. 98 (8): 821-7. -
Gonçalves J et al. (2016) Potential of mannan or dextrin nanogels as vaccine carrier/adjuvant systems
Journal of Bioactive and Compatible Polymers. Mar 14 [Epub ahead of print] -
Gonçalves J et al. (2017) Extended-access methamphetamine self-administration elicits neuroinflammatory response along with blood-brain barrier breakdown.
Brain Behav Immun. 62: 306-317. -
Palin, N.K. et al. (2017) Activin inhibition limits early innate immune response in rat kidney allografts-a pilot study.
Transpl Int. 30 (1): 96-107.
