CD169 antibody | ED3
Mouse anti Rat CD169 antibody, clone ED3 (MCA343GA) used to identify CD169 expressing cells in rat spleen by innunohistochemistry on cryosections.
Image caption:
Immunostaining of rat spleen. Serial-cut frozen sections were stained with mAbs towards A, rat MCL B, rat CD169 C, rat MHC class II D, human MHC class I (negative control) and visualized with peroxidase conjugated secondary antibody and DAB substrate. RP: red pulp. PALS: periarteriolar lymphoid sheath. FOLL: follicle. MZ: marginal zone.
From: Lobato-Pascual A, Saether PC, Dahle MK, Gaustad P, Dissen E, et al. (2013)
Rat Macrophage C-Type Lectin Is an Activating Receptor Expressed by Phagocytic Cells.
PLoS ONE 8(2): e57406.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Systemically administered liposomes home primarily to splenic marginal zone and red pulp macrophages. (a,b) Healthy rats were injected with 5 mg/kg DiI-labeled PCLs and PSLs. Splenic cryosections were stained with CD169 (a, marginal metallophilic and marginal zone macrophages) and CD68 (b, red pulp macrophages). One representative experiment is shown (20× magnification).
From: Bogie JF, Jorissen W, Mailleux J, Nijland PG, Zelcer N, Vanmierlo T, Van Horssen J, Stinissen P, Hellings N, Hendriks JJ. Myelin alters the inflammatory phenotype of macrophages by activating PPARs. Acta Neuropathol Commun. 2013 Aug 2;1(1):43.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Rat CD169 antibody, clone ED3 (MCA343GA) used to identify CD169 expressing cells in rat spleen by innunohistochemistry on cryosections.
Image caption:
Immunostaining of rat spleen. Serial-cut frozen sections were stained with mAbs towards A, rat MCL B, rat CD169 C, rat MHC class II D, human MHC class I (negative control) and visualized with peroxidase conjugated secondary antibody and DAB substrate. RP: red pulp. PALS: periarteriolar lymphoid sheath. FOLL: follicle. MZ: marginal zone.
From: Lobato-Pascual A, Saether PC, Dahle MK, Gaustad P, Dissen E, et al. (2013)
Rat Macrophage C-Type Lectin Is an Activating Receptor Expressed by Phagocytic Cells.
PLoS ONE 8(2): e57406.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Systemically administered liposomes home primarily to splenic marginal zone and red pulp macrophages. (a,b) Healthy rats were injected with 5 mg/kg DiI-labeled PCLs and PSLs. Splenic cryosections were stained with CD169 (a, marginal metallophilic and marginal zone macrophages) and CD68 (b, red pulp macrophages). One representative experiment is shown (20× magnification).
From: Bogie JF, Jorissen W, Mailleux J, Nijland PG, Zelcer N, Vanmierlo T, Van Horssen J, Stinissen P, Hellings N, Hendriks JJ. Myelin alters the inflammatory phenotype of macrophages by activating PPARs. Acta Neuropathol Commun. 2013 Aug 2;1(1):43.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Rat CD169 antibody, clone ED3 (MCA343R) used to label macrophages in rat kidney by immunohistochemistry on formalin fixed, paraffin embedded tissue sections
Image caption:
Anti-inflammatory effect of EC2319. (A) Immunohistochemistry stained for CD169+ monocyte/macrophages, infiltrates of kidney sections of rats with anti-GBM GN.
From: Garcia GE, Lu YJ, Truong LD, Roncal-Jiménez CA, Miyazaki M, Miyazaki-Anzai S, Cara-Fuentes G, Andres-Hernando A, Lanaspa M, Johnson RJ, Leamon CP.
A Novel Treatment for Glomerular Disease: Targeting the Activated Macrophage Folate Receptor with a Trojan Horse Therapy in Rats.
Cells. 2021 Aug 17;10(8):2113.
doi: 10.3390/cells10082113.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
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C IF P ResetMouse anti Rat CD169
- Product Type
- Monoclonal Antibody
- Clone
- ED3
- Isotype
- IgG2a
- Specificity
- CD169
Antibody Quality and Performance Guarantee
| Mouse anti rat CD169 antibody, clone ED3 recognises the rat CD169 cell surface antigen, a ~185 kDa molecule expressed by macrophages, predominately confined to lymphoid organs only. Monocytes and granulocytes are negative. No other cell types are positive. The most conspicious property of ED3 is it stains marginal zone macrophages and marginal metallophils in the spleen very strongly. Furthermore, macrophages in (auto-immune) diseased tissues express the ED3 antigen. In healthy tissue no expression occurs. CD169 is a receptor for glycoconjugates containing sialic acid. |
- Target Species
- Rat
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- Rat Spleen cell homogenate
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0-Ag 14 mouse myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/500 | |
| Immunofluorescence | |
||
| Immunohistology - Frozen | |
1/50 | 1/250 |
| Immunoprecipitation | |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG2a Negative Control | MCA1210 | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG2a Negative Control | ||||||
Source Reference
-
Dijkstra, C.D. et al. (1985) The heterogeneity of mononuclear phagocytes in lymphoid organs: distinct macrophage subpopulations in the rat recognized by monoclonal antibodies ED1, ED2 and ED3.
Immunology. 54 (3): 589-99.
References for CD169 antibody
-
van Rees, E.P. et al. (1985) The postnatal development of cell populations in the rat popliteal lymph node. An immunohistochemical study.
Cell Tissue Res. 242 (2): 391-8. -
van den Berg, T.K. et al. (1992) Sialoadhesin on macrophages: its identification as a lymphocyte adhesion molecule.
J Exp Med. 176 (3): 647-55. -
Allen, A.R. et al. (1999) Endothelial expression of VCAM-1 in experimental crescentic nephritis and effect of antibodies to very late antigen-4 or VCAM-1 on glomerular injury.
J Immunol. 162: 5519-27. -
Richards, P.J. et al. (1999) Liposomal clodronate eliminates synovial macrophages, reduces inflammation and ameliorates joint destruction in antigen-induced arthritis.
Rheumatology (Oxford). 38: 818-25. -
Ikezumi, Y. et al. (2000) An anti-CD5 monoclonal antibody ameliorates proteinuria and glomerular lesions in rat mesangioproliferative glomerulonephritis.
Kidney Int. 58: 100-14. -
Nakamura, K. et al. (2002) Lymph node macrophages, but not spleen macrophages, express high levels of unmasked sialoadhesin: implication for the adhesive properties of macrophages in vivo.
Glycobiology. 12: 209-16. -
Camelo, S. et al. (2004) The distribution of antigen in lymphoid tissues following its injection into the anterior chamber of the rat eye.
J Immunol. 172: 5388-95. -
Homo-Delarche, F. et al. (2006) Islet inflammation and fibrosis in a spontaneous model of type 2 diabetes, the GK rat.
Diabetes. 55: 1625-33.
View The Latest Product References
-
Camelo, S. et al. (2006) Antigen from the anterior chamber of the eye travels in a soluble form to secondary lymphoid organs via lymphatic and vascular routes.
Invest Ophthalmol Vis Sci. 47: 1039-46. -
Fujita, E. et al. (2010) Statin Attenuates Experimental Anti-Glomerular Basement Membrane Glomerulonephritis Together with the Augmentation of Alternatively Activated Macrophages.
Am J Pathol. 177: 1143-54. -
Savikko, J. et al. (2011) Early short-term imatinib treatment is sufficient to prevent the development of chronic allograft nephropathy.
Nephrol Dial Transplant. 26 (9): 3026-32. -
Lobato-Pascual, A. et al. (2013) Rat macrophage C-type lectin is an activating receptor expressed by phagocytic cells.
PLoS One. 8: e57406. -
Rintala, J.M. et al. (2014) Epidermal growth factor inhibition, a novel pathway to prevent chronic allograft injury.
Transplantation. 98 (8): 821-7. -
Palin, N.K. et al. (2015) Intensive perioperative simvastatin treatment protects from chronic kidney allograft injury.
Am J Nephrol. 41 (4-5): 383-91. -
Rintala, J.M. et al. (2016) Epidermal growth factor receptor inhibition with erlotinib ameliorates anti-Thy 1.1-induced experimental glomerulonephritis.
J Nephrol. 29 (3): 359-65. -
Rintala, J.M. et al. (2016) Oral Platelet-Derived Growth Factor and Vascular Endothelial Growth Factor Inhibitor Sunitinib Prevents Chronic Allograft Injury in Experimental Kidney Transplantation Model.
Transplantation. 100 (1): 103-10. -
Gonçalves, C. et al. (2016) Potential of mannan or dextrin nanogels as vaccine carrier/adjuvant systems
J Bioactive Compat Polymers. 31 (5): 453-66. -
Palin, N.K. et al. (2017) Activin inhibition limits early innate immune response in rat kidney allografts-a pilot study.
Transpl Int. 30 (1): 96-107. -
Gonçalves J et al. (2017) Extended-access methamphetamine self-administration elicits neuroinflammatory response along with blood-brain barrier breakdown.
Brain Behav Immun. 62: 306-317. -
Garcia, G.E. et al. (2021) A Novel Treatment for Glomerular Disease: Targeting the Activated Macrophage Folate Receptor with a Trojan Horse Therapy in Rats.
Cells. 10(8): 2113.
- Synonyms
- ED3
- RRID
- AB_2189149
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