CD169 antibody | ED3
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Mouse anti Rat CD169
- Product Type
- Monoclonal Antibody
|Mouse anti rat CD169 antibody, clone ED3 recognises the rat CD169 cell surface antigen, a ~185 kDa molecule expressed by macrophages, predominately confined to lymphoid organs only. Monocytes and granulocytes are negative. No other cell types are positive. The most conspicious property of ED3 is it stains marginal zone macrophages and marginal metallophils in the spleen very strongly. Furthermore, macrophages in (auto-immune) diseased tissues express the ED3 antigen. In healthy tissue no expression occurs. CD169 is a receptor for glycoconjugates containing sialic acid.|
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide
- Carrier Free
- Rat Spleen cell homogenate
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0-Ag 14 mouse myeloma cell line.
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Frozen||1/50||1/250|
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Dijkstra, C.D. et al. (1985) The heterogeneity of mononuclear phagocytes in lymphoid organs: distinct macrophage subpopulations in the rat recognized by monoclonal antibodies ED1, ED2 and ED3.
Immunology. 54 (3): 589-99.
References for CD169 antibody
van Rees, E.P. et al. (1985) The postnatal development of cell populations in the rat popliteal lymph node. An immunohistochemical study.
Cell Tissue Res. 242 (2): 391-8.
van den Berg, T.K. et al. (1992) Sialoadhesin on macrophages: its identification as a lymphocyte adhesion molecule.
J Exp Med. 176 (3): 647-55.
Allen, A.R. et al. (1999) Endothelial expression of VCAM-1 in experimental crescentic nephritis and effect of antibodies to very late antigen-4 or VCAM-1 on glomerular injury.
J Immunol. 162: 5519-27.
Richards, P.J. et al. (1999) Liposomal clodronate eliminates synovial macrophages, reduces inflammation and ameliorates joint destruction in antigen-induced arthritis.
Rheumatology (Oxford). 38: 818-25.
Ikezumi, Y. et al. (2000) An anti-CD5 monoclonal antibody ameliorates proteinuria and glomerular lesions in rat mesangioproliferative glomerulonephritis.
Kidney Int. 58: 100-14.
Nakamura, K. et al. (2002) Lymph node macrophages, but not spleen macrophages, express high levels of unmasked sialoadhesin: implication for the adhesive properties of macrophages in vivo.
Glycobiology. 12: 209-16.
Camelo, S. et al. (2004) The distribution of antigen in lymphoid tissues following its injection into the anterior chamber of the rat eye.
J Immunol. 172: 5388-95.
Homo-Delarche, F. et al. (2006) Islet inflammation and fibrosis in a spontaneous model of type 2 diabetes, the GK rat.
Diabetes. 55: 1625-33.
View The Latest Product References
Camelo, S. et al. (2006) Antigen from the anterior chamber of the eye travels in a soluble form to secondary lymphoid organs via lymphatic and vascular routes.
Invest Ophthalmol Vis Sci. 47: 1039-46.
Fujita, E. et al. (2010) Statin Attenuates Experimental Anti-Glomerular Basement Membrane Glomerulonephritis Together with the Augmentation of Alternatively Activated Macrophages.
Am J Pathol. 177: 1143-54.
Savikko, J. et al. (2011) Early short-term imatinib treatment is sufficient to prevent the development of chronic allograft nephropathy.
Nephrol Dial Transplant. 26 (9): 3026-32.
Lobato-Pascual, A. et al. (2013) Rat macrophage C-type lectin is an activating receptor expressed by phagocytic cells.
PLoS One. 8: e57406.
Rintala, J.M. et al. (2014) Epidermal growth factor inhibition, a novel pathway to prevent chronic allograft injury.
Transplantation. 98 (8): 821-7.
Palin, N.K. et al. (2015) Intensive perioperative simvastatin treatment protects from chronic kidney allograft injury.
Am J Nephrol. 41 (4-5): 383-91.
Rintala, J.M. et al. (2016) Epidermal growth factor receptor inhibition with erlotinib ameliorates anti-Thy 1.1-induced experimental glomerulonephritis.
J Nephrol. 29 (3): 359-65.
Rintala, J.M. et al. (2016) Oral Platelet-Derived Growth Factor and Vascular Endothelial Growth Factor Inhibitor Sunitinib Prevents Chronic Allograft Injury in Experimental Kidney Transplantation Model.
Transplantation. 100 (1): 103-10.
Gonçalves, C. et al. (2016) Potential of mannan or dextrin nanogels as vaccine carrier/adjuvant systems
J Bioactive Compat Polymers. 31 (5): 453-66.
Palin, N.K. et al. (2017) Activin inhibition limits early innate immune response in rat kidney allografts-a pilot study.
Transpl Int. 30 (1): 96-107.
Gonçalves J et al. (2017) Extended-access methamphetamine self-administration elicits neuroinflammatory response along with blood-brain barrier breakdown.
Brain Behav Immun. 62: 306-317.
Garcia, G.E. et al. (2021) A Novel Treatment for Glomerular Disease: Targeting the Activated Macrophage Folate Receptor with a Trojan Horse Therapy in Rats.
Cells. 10(8): 2113.
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