CD11b antibody | ED8

Product Code Applications Pack Size List Price Your Price Qty
MCA619R
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SDS Safety Datasheet SDS
C * F 0.25 mg loader
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Mouse anti Rat CD11b antibody, clone ED8 recognizes a membrane antigen on rat macrophages, monocytes, dendritic cells and granulocytes. It also recognizes small ramified microglia in the central nervous system. No other cell types are positive for ED8. The recognized antigen is a heterodimer consisting of ~160 and ~95 kDa, belonging to the family of adhesion molecules CD11b/CD18, also designated as Mac-1 antigen or CR3. Mouse anti Rat CD11b antibody, clones ED7 (MCA618R) and ED8 may recognize closely related epitopes on the same molecule. Both clones ED7 and ED8 induce homotypic aggregation of granulocytes (Drasković-Pavlović et al. 1999).

Target Species
Rat
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Rat spleen cell homogenate with Freund's complete adjuvant.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BAL/B/c mice were fused with cells of the Sp2/0 Ag-14 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Immunohistology - Frozen 1
Immunohistology - Paraffin
  1. 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
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Description Mouse IgG1 Negative Control

References for CD11b antibody

  1. Damoiseaux, J.G. et al. (1989) Heterogeneity of macrophages in the rat evidenced by variability in determinants: two new anti-rat macrophage antibodies against a heterodimer of 160 and 95 kd (CD11/CD18).
    J Leukoc Biol. 46 (6): 556-64.
  2. de Groot, C.J. et al. (1988) Discrimination between different types of neuroglial cells in rat central nervous system using combined immuno- and enzyme-histochemical methods.
    Immunobiology. 178 (3): 177-90.
  3. Dijkstra, C.D. & Damoiseaux, J.G. (1993) Macrophage heterogeneity established by immunocytochemistry.
    Prog Histochem Cytochem. 27 (2): 1-65.
  4. Huitinga, I. et al. (1993) Treatment with anti-CR3 antibodies ED7 and ED8 suppresses experimental allergic encephalomyelitis in Lewis rats.
    Eur J Immunol. 23 (3): 709-15.
  5. Zilka, N. et al. (2009) Human misfolded truncated tau protein promotes activation of microglia and leukocyte infiltration in the transgenic rat model of tauopathy.
    J Neuroimmunol. 209 (1-2): 16-25.
  6. Drasković-Pavlović, B. et al. (1999) Differential effects of anti-rat CD11b monoclonal antibodies on granulocyte adhesiveness.
    Immunology. 96: 83-9.
  7. Dong, H. et al. (2014) Lithium ameliorates lipopolysaccharide-induced microglial activation via inhibition of toll-like receptor 4 expression by activating the PI3K/Akt/FoxO1 pathway.
    J Neuroinflammation. 11: 140.
  8. Nacka-Aleksić M et al. (2015) Sexual dimorphism in the aged rat CD4+ T lymphocyte-mediated immune response elicited by inoculation with spinal cord homogenate.
    Mech Ageing Dev. 152: 15-31.
  9. View The Latest Product References
  10. Stojić-Vukanić Z et al. (2015) Aging diminishes the resistance of AO rats to EAE: putative role of enhanced generation of GM-CSF Expressing CD4+ T cells in aged rats.
    Immun Ageing. 12: 16.
  11. Stojić-Vukanić, Z. et al. (2016) Estradiol enhances capacity of TLR-matured splenic dendritic cells to polarize CD4+ lymphocytes into IL-17/GM-CSF-producing cells in vitro.
    Int Immunopharmacol. 40: 244-53.
  12. Pilipović, I. et al. (2020) Propranolol diminished severity of rat EAE by enhancing immunoregulatory/protective properties of spinal cord microglia.
    Neurobiol Dis. 134: 104665.
  13. Nacka-Aleksić, M. et al. (2020) Sex as a confounding factor in the effects of ageing on rat lymph node t cell compartment.
    Exp Gerontol. 142: 111140.
  14. Djuretić, J. et al. (2021) Infrared radiation from cage bedding moderates rat inflammatory and autoimmune responses in collagen-induced arthritis.
    Sci Rep. 11 (1): 2882.
  15. Pilipović, I. et al. (2019) Noradrenaline modulates CD4+ T cell priming in rat experimental autoimmune encephalomyelitis: a role for the α1-adrenoceptor.
    Immunol Res. 67 (2-3): 223-40.
  16. Anderson, L.E. et al. (2021) Injection of Micronized Human Amnion/Chorion Membrane Results in Increased Early Supraspinatus Muscle Regeneration in a Chronic Model of Rotator Cuff Tear.
    Ann Biomed Eng. Nov 11 [Epub ahead of print].

Functional Assays

Synonyms
Integrin Alpha M Chain
MAC-1
RRID
AB_10671912
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