CD11b antibody | ED8
Lithium inhibits lipopolysaccharide (LPS)-induced microglial activation. Primary microglial cells were pretreated with LiCl at 0.3, 1 and 3 mM for 30 minutes and then stimulated with LPS at 10 ng/ml for 24 hours. (a) and (b) For flow cytometric analysis, the cells were incubated with PE-conjugated ED8 antibody at 37°C for 1 hour. **P <0.01 versus the response to medium alone. #P <0.05, ##P <0.01 versus LPS treatment groups. The data are presented as the mean ± s.e.m. of three independent experiments. (c) The cells were stained with ED8 antibody and upregulated ED8-immunopositive expression (green) on the activated microglia was observed using confocal scanning. The blue staining represents DAPI. Scale bar 50 μm.
From: Dong H, Zhang X, Dai X, Lu S, Gui B, Jin W, Zhang S, Zhang S, Qian Y. Lithium ameliorates lipopolysaccharide-induced microglial activation via inhibition of toll-like receptor 4 expression by activating the PI3K/Akt/FoxO1 pathway. J Neuroinflammation. 2014 Aug 14;11:140.
Image caption:
TLR4 regulates microglial activation. Primary microglial cells were transfected with TLR4 siRNA (100 nM) or control (siControl) for 48 hours and then stimulated with lipopolysaccharide (LPS) (10 ng/ml) for 24 hours. (a) Suppression of TLR4 expression by TLR4 siRNA. (b) Quantification of IL-6 and TNF-α production in media, **P <0.01 versus the response to medium alone. ##P <0.01 versus LPS treatment groups. The data are presented as the mean ± s.e.m. of three independent experiments. (c) The cells were stained with ED8 antibody and upregulated ED8-immunopositive expression (green) on activated microglia was observed using confocal. The blue staining represents DAPI. Scale bar 50 μm.
From: Dong H, Zhang X, Dai X, Lu S, Gui B, Jin W, Zhang S, Zhang S, Qian Y. Lithium ameliorates lipopolysaccharide-induced microglial activation via inhibition of toll-like receptor 4 expression by activating the PI3K/Akt/FoxO1 pathway. J Neuroinflammation. 2014 Aug 14;11:140.
Mouse anti Rat CD11b antibody, clone ED8 used for the evaluation of CD11b expression on spinal cord mononuclear cells by flow cytometry.
Image caption:
Aging increases the number of all spinal cord mononuclear cells, and CD45 int/hi CD11b+ nonlymphoid cells, recovered on the 7 th d.p.i. from AO rat spinal cord. (a) Bar graph indicates the total number of mononuclear cells (MNC) isolated from spinal cord of young and aged rats on the 7 th d.p.i. (Panel b) Flow cytometry density plots indicate the expression of CD45 on CD11b+ cells from spinal cord of (left) young and (right) aged rats on the 7 th d.p.i. CD11b + cells were gated as shown in the flow cytometry histograms. Numbers in the flow cytometry histograms represent the percentage of CD11b+ cells. R1 = CD11b+ CD45 hi cells; R2 = CD11b+ CD45 int cells. Numbers in the table indicate the percentage of cells in the specified region (R). Bar graph indicates the number of CD11b+ cells in spinal cords from young and aged rats. (Panel c) Bar graphs indicate the number of (left) CD11b+ CD45 int and (right) CD11b+ CD45 hi cells in spinal cords from young and aged rats on the 7 th d.p.i. (d) Bar graph indicates the fold change in expression of mRNA for SOCS1 in spinal cord mononuclear cells from aged relative to young rats on the 7 th d.p.i. as determined by RT-qPCR. Data are normalized to β-actin (ACTB). All results are presented as means ±SEM (n = 9/group). Data are representative of one of two experiments with similar results. *p <0.05; ***p < 0.001
From: Stojić-Vukanić Z, Nacka-Aleksić M, Pilipović I, Vujnović I, Blagojević V, Kosec D, Dimitrijević M, Leposavić G.
Aging diminishes the resistance of AO rats to EAE: putative role of enhanced generation of GM-CSF Expressing CD4+ T cells in aged rats.
Immun Ageing. 2015 Oct 6;12:16.
Mouse anti Rat CD11b antibody, clone ED8 recognizes a membrane antigen on rat macrophages, monocytes, dendritic cells and granulocytes. It also recognizes small ramified microglia in the central nervous system. No other cell types are positive for ED8. The recognized antigen is a heterodimer consisting of ~160 and ~95 kDa, belonging to the family of adhesion molecules CD11b/CD18, also designated as Mac-1 antigen or CR3. Mouse anti Rat CD11b antibody, clones ED7 (MCA618R) and ED8 may recognize closely related epitopes on the same molecule. Both clones ED7 and ED8 induce homotypic aggregation of granulocytes (Drasković-Pavlović et al. 1999).
Product Details
- Target Species
- Rat
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Reconstitute with 1 ml distilled water
- Preparation
- Purified IgG prepared by ion exchange chromatography
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by ion exchange chromatography
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Carrier Free
- Yes
- Immunogen
- Rat spleen cell homogenate with Freund's complete adjuvant.
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BAL/B/c mice were fused with cells of the Sp2/0 Ag-14 myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 12 months from date of reconstitution.
More Information
- Regulatory
- For research purposes only
Applications of CD11b antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | ||
| Immunohistology - Paraffin | |||
| Immunohistology - Frozen 1 | |||
| Immunohistology - Paraffin | |||
| Flow Cytometry | Neat |
- 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control:FITC | MCA1209F | 0.1 mg | F | ||
| Mouse IgG1 Negative Control:RPE | MCA1209PE | 100 Tests | F |
Product Specific References
References for CD11b antibody
-
Damoiseaux, J.G. et al. (1989) Heterogeneity of macrophages in the rat evidenced by variability in determinants: two new anti-rat macrophage antibodies against a heterodimer of 160 and 95 kd (CD11/CD18).
J Leukoc Biol. 46 (6): 556-64. -
de Groot, C.J. et al. (1988) Discrimination between different types of neuroglial cells in rat central nervous system using combined immuno- and enzyme-histochemical methods.
Immunobiology. 178 (3): 177-90. -
Dijkstra, C.D. & Damoiseaux, J.G. (1993) Macrophage heterogeneity established by immunocytochemistry.
Prog Histochem Cytochem. 27 (2): 1-65. -
Huitinga, I. et al. (1993) Treatment with anti-CR3 antibodies ED7 and ED8 suppresses experimental allergic encephalomyelitis in Lewis rats.
Eur J Immunol. 23 (3): 709-15. -
Zilka, N. et al. (2009) Human misfolded truncated tau protein promotes activation of microglia and leukocyte infiltration in the transgenic rat model of tauopathy.
J Neuroimmunol. 209 (1-2): 16-25. -
Drasković-Pavlović, B. et al. (1999) Differential effects of anti-rat CD11b monoclonal antibodies on granulocyte adhesiveness.
Immunology. 96: 83-9. -
Dong, H. et al. (2014) Lithium ameliorates lipopolysaccharide-induced microglial activation via inhibition of toll-like receptor 4 expression by activating the PI3K/Akt/FoxO1 pathway.
J Neuroinflammation. 11: 140. -
Nacka-Aleksić M et al. (2015) Sexual dimorphism in the aged rat CD4+ T lymphocyte-mediated immune response elicited by inoculation with spinal cord homogenate.
Mech Ageing Dev. 152: 15-31. -
Stojić-Vukanić Z et al. (2015) Aging diminishes the resistance of AO rats to EAE: putative role of enhanced generation of GM-CSF Expressing CD4+ T cells in aged rats.
Immun Ageing. 12: 16. -
Stojić-Vukanić, Z. et al. (2016) Estradiol enhances capacity of TLR-matured splenic dendritic cells to polarize CD4+ lymphocytes into IL-17/GM-CSF-producing cells in vitro.
Int Immunopharmacol. 40: 244-53.
