CD11b antibody | ED8










Product Details
- Target Species
- Rat
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Reconstitute with 1 ml distilled water
- Preparation
- Purified IgG prepared by ion exchange chromatography
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by ion exchange chromatography
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Carrier Free
- Yes
- Immunogen
- Rat spleen cell homogenate with Freund's complete adjuvant.
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BAL/B/c mice were fused with cells of the Sp2/0 Ag-14 myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of CD11b antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | Neat | ||
Immunohistology - Paraffin | |||
Flow Cytometry | |||
Immunohistology - Frozen 1 | |||
Immunohistology - Paraffin | |||
Flow Cytometry | Neat |
- 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Secondary Antibodies Available
Negative Isotype Controls Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Mouse IgG1 Negative Control:FITC | MCA1209F | F | 0.1 mg |
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Mouse IgG1 Negative Control | MCA1209 | F | 0.1 mg |
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Mouse IgG1 Negative Control:RPE | MCA1209PE | F | 100 Tests |
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Product Specific References
References for CD11b antibody
-
Damoiseaux, J.G. et al. (1989) Heterogeneity of macrophages in the rat evidenced by variability in determinants: two new anti-rat macrophage antibodies against a heterodimer of 160 and 95 kd (CD11/CD18).
J Leukoc Biol. 46 (6): 556-64. -
de Groot, C.J. et al. (1988) Discrimination between different types of neuroglial cells in rat central nervous system using combined immuno- and enzyme-histochemical methods.
Immunobiology. 178 (3): 177-90. -
Dijkstra, C.D. & Damoiseaux, J.G. (1993) Macrophage heterogeneity established by immunocytochemistry.
Prog Histochem Cytochem. 27 (2): 1-65. -
Huitinga, I. et al. (1993) Treatment with anti-CR3 antibodies ED7 and ED8 suppresses experimental allergic encephalomyelitis in Lewis rats.
Eur J Immunol. 23 (3): 709-15. -
Zilka, N. et al. (2009) Human misfolded truncated tau protein promotes activation of microglia and leukocyte infiltration in the transgenic rat model of tauopathy.
J Neuroimmunol. 209 (1-2): 16-25. -
Drasković-Pavlović, B. et al. (1999) Differential effects of anti-rat CD11b monoclonal antibodies on granulocyte adhesiveness.
Immunology. 96: 83-9. -
Dong, H. et al. (2014) Lithium ameliorates lipopolysaccharide-induced microglial activation via inhibition of toll-like receptor 4 expression by activating the PI3K/Akt/FoxO1 pathway.
J Neuroinflammation. 11: 140. -
Nacka-Aleksić M et al. (2015) Sexual dimorphism in the aged rat CD4+ T lymphocyte-mediated immune response elicited by inoculation with spinal cord homogenate.
Mech Ageing Dev. 152: 15-31. -
Stojić-Vukanić Z et al. (2015) Aging diminishes the resistance of AO rats to EAE: putative role of enhanced generation of GM-CSF Expressing CD4+ T cells in aged rats.
Immun Ageing. 12: 16. -
Stojić-Vukanić, Z. et al. (2016) Estradiol enhances capacity of TLR-matured splenic dendritic cells to polarize CD4+ lymphocytes into IL-17/GM-CSF-producing cells in vitro.
Int Immunopharmacol. 40: 244-53. -
Pilipović I et al. (2019) Propranolol diminished severity of rat EAE by enhancing immunoregulatory/protective properties of spinal cord microglia.
Neurobiol Dis. Nov 2 [Epub ahead of print]. -
Nacka-Aleksić et al. (2020) Sex as a confounding factor in the effects of ageing on rat lymph node t cell compartment
Exp Gerontol. Oct 28 [Epub ahead of print]
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