IgM antibody | NRBM
Mouse anti Rabbit IgM (B Cell Marker):FITC
Mouse anti Rabbit IgM (B Cell Marker)
Mouse anti Rabbit IgM (B Cell Marker):RPE
- Product Type
- Monoclonal Antibody
- Clone
- NRBM
- Isotype
- IgG1
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| MCA812F | F | 0.1 mg | ||||
| MCA812GA | C F | 0.1 mg | ||||
| MCA812PE | F | 100 Tests |
Mouse anti Rabbit IgM (B Cell Marker) antibody, clone NRBM specifically recognizes the μ chain of rabbit Ig.
Mammalian IgM is produced and secreted by plasma cells located in bone marrow, lymph nodes and spleen. IgM is present in both a secreted polymeric form and as cell surface monomeric form on B cells.
Clone NRBM has been used in number of studies to label IgM+ B cells (Dewals et al. 2011, Waclavicek et al. 2009) and as such can be considered a reliable marker of lagomorph (rabbit) B cells in flow cytometry.
Mammalian IgM is produced and secreted by plasma cells located in bone marrow, lymph nodes and spleen. IgM is present in both a secreted polymeric form and as cell surface monomeric form on B cells.
Clone NRBM has been used in number of studies to label IgM+ B cells (Dewals et al. 2011, Waclavicek et al. 2009) and as such can be considered a reliable marker of lagomorph (rabbit) B cells in flow cytometry.
Product Details
- Target Species
- Rabbit
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Reconstitute with 1.0 ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose - Carrier Free
- Yes
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Fusion Partners
- Spleen cells from immunised mice were fused with cells of the Mouse P3X63Ag8.653 myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Prior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. - Guarantee
- 18 months from date of despatch.
- Guarantee
- 18 months from date of despatch.
- Guarantee
- 12 months from date of reconstitution
More Information
- UniProt
- P04221 Related reagents
- P03988 Related reagents
- GO Terms
- GO:0003823 antigen binding
- GO:0005886 plasma membrane
- GO:0016021 integral to membrane
- Regulatory
- For research purposes only
Applications of IgM antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | 1/10 | |
| Flow Cytometry | |||
| Immunohistology - Frozen | |||
| Flow Cytometry | Neat |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Useful Reagents Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse anti Rabbit T Lymphocytes:FITC | MCA800F | 100 Tests | F |
Product Specific References
References for IgM antibody
-
Idogawa, H. et al. (1997) Progression of articular destruction and the production of tumour necrosis factor-alpha in antigen-induced arthritis in rabbits.
Scand J Immunol. 46 (6): 572-80. -
Dewals, B. et al. (2008) Malignant catarrhal fever induced by alcelaphine herpesvirus 1 is associated with proliferation of CD8+ T cells supporting a latent infection.
PLoS One 3: e1627. -
Gillet, L. et al. (2009) Anchoring tick salivary anti-complement proteins IRAC I and IRAC II to membrane increases their immunogenicity.
Vet Res. 40: 51. -
Stich, N. et al. (2010) Staphylococcal Superantigen (TSST-1) Mutant Analysis Reveals that T Cell Activation Is Required for Biological Effects in the Rabbit Including the Cytokine Storm.
Toxins 2: 2272-88. -
Waclavicek, M. et al. (2009) Analysis of the early response to TSST-1 reveals Vbeta-unrestricted extravasation, compartmentalization of the response, and unresponsiveness but not anergy to TSST-1.
J Leukoc Biol. 85 (1): 44-54. -
Anderson, I.E. et al. (2008) Production and utilization of interleukin-15 in malignant catarrhal fever.
J Comp Pathol. 138: 131-44. -
Dewals, B.G. and Vanderplasschen, A. (2011) Malignant catarrhal fever induced by Alcelaphine herpesvirus 1 is characterized by an expansion of activated CD3+CD8+CD4- T cells expressing a cytotoxic phenotype in both lymphoid and non-lymphoid tissues.
Vet Res. 42: 95. -
Dewals, B. et al. (2011) Ex vivo bioluminescence detection of alcelaphine herpesvirus 1 infection during malignant catarrhal fever.
J Virol. 85: 6941-54. -
Milanovic, V. et al. (2017) Histological and immunological changes in uterus during the different reproductive stages at Californian rabbit (Oryctolagus cuniculus).
Kafkas Univ Vet Fak Derg, 23, 137-44. -
Ondruska, L. et al. (2016) Decrease in C-reactive protein levels in rabbits after vaccination with a live attenuated myxoma virus vaccine.
Veterinární Medicína. 61 (No. 10): 571-6. -
Myster, F. et al. (2015) Viral semaphorin inhibits dendritic cell phagocytosis and migration but is not essential for gammaherpesvirus-induced lymphoproliferation in malignant catarrhal fever.
J Virol. 89 (7): 3630-47. -
Sorel, O. et al. (2017) Macavirus latency-associated protein evades immune detection through regulation of protein synthesis in cis depending upon its glycin/glutamate-rich domain.
PLoS Pathog. 13 (10): e1006691.
