CD8 Alpha antibody | 11/295/33
Mouse anti Pig CD8 Alpha:Alexa Fluor® 647
Mouse anti Pig CD8 Alpha:FITC
Mouse anti Pig CD8 Alpha
Mouse anti Pig CD8 Alpha:RPE
- Product Type
- Monoclonal Antibody
- Clone
- 11/295/33
- Isotype
- IgG2a
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| MCA6048A647 | F | 100 Tests/1ml | ||||
| MCA6048F | F | 0.1 mg | ||||
| MCA6048GA | F | 0.1 mg | ||||
| MCA6048PE | F | 100 Tests |
Mouse anti Pig CD8 alpha antibody, clone 11/295/33 recognizes an epitope on the alpha chain of porcine CD8. Clone 11/295/33 detects a different epitope from that bound by clone 76-2-11.
In addition to thymocytes and cytotoxic T cells, CD8 alpha is expressed on porcine NK cells. Swine CD8 alpha+CD3- NK cells are divided into NKp46+ (CD335) and NKp46- cells. A further CD8 alpha dim/- NKp46 high NK cell population can be found in porcine spleen and liver (Mair et al. 2012, 2013). Pigs are also remarkable in having significant levels of peripheral CD4 CD8 double positive T cells (Saalmüller et al. 1987; Zuckermann 1999).
In addition to thymocytes and cytotoxic T cells, CD8 alpha is expressed on porcine NK cells. Swine CD8 alpha+CD3- NK cells are divided into NKp46+ (CD335) and NKp46- cells. A further CD8 alpha dim/- NKp46 high NK cell population can be found in porcine spleen and liver (Mair et al. 2012, 2013). Pigs are also remarkable in having significant levels of peripheral CD4 CD8 double positive T cells (Saalmüller et al. 1987; Zuckermann 1999).
Product Details
- Target Species
- Pig
- Product Form
- Purified IgG conjugated to Alexa Fluor 647 - liquid
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Reconstitute with 1.0 ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose - Carrier Free
- Yes
- Immunogen
- Porcine lymphocytes
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 18 months from date of despatch
- Guarantee
- 12 months from date of reconstitution
More Information
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Regulatory
- For research purposes only
Applications of CD8 Alpha antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | ||
| Flow Cytometry | Neat | 1/10 | |
| Flow Cytometry | |||
| Flow Cytometry | Neat | 1/10 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG2a Negative Control:Alexa Fluor® 647 | MCA929A647 | 100 Tests/1ml | F | ||
| Mouse IgG2a Negative Control:FITC | MCA929F | 100 Tests | F | ||
| Mouse IgG2a Negative Control | MCA929 | 100 Tests | F | ||
| Mouse IgG2a Negative Control:RPE | MCA929PE | 100 Tests | F |
Useful Reagents Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse anti Pig CD3:RPE | MCA5951PE | 100 Tests | F | ||
| Mouse anti Pig CD8 Beta Chain:RPE | MCA5954PE | 100 Tests | F | ||
| Mouse anti Pig CD4:FITC | MCA6045F | 0.1 mg | F IF | ||
| Mouse anti Pig CD3:RPE | MCA5951PE | 100 Tests | F | ||
| Mouse anti Pig CD8 Beta Chain:RPE | MCA5954PE | 100 Tests | F | ||
| Mouse anti Pig CD4:Alexa Fluor® 647 | MCA6045A647 | 100 Tests | F | ||
| Mouse anti Pig CD3 | MCA5951GA | 0.1 mg | C F IP WB | ||
| Mouse anti Pig CD8 Beta Chain | MCA5954GA | 0.1 mg | F | ||
| Mouse anti Pig CD4 | MCA6045GA | 0.1 mg | F | ||
| Mouse anti Pig CD45RC | MCA6049GA | 0.1 mg | F | ||
| Mouse anti Pig CD8 Beta Chain:FITC | MCA5954F | 0.1 mg | F | ||
| Mouse anti Pig CD4:Alexa Fluor® 647 | MCA6045A647 | 100 Tests | F |
Product Specific References
Further Reading
-
Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
Vet Res. 39: 54.
