CD6 antibody | MIL8
Mouse anti Pig wCD6, clone MIL8 reacts with pig wCD6, the porcine homolog of human CD6. Clone MIL8 was clustered as wCD6 at the Second International Swine CD Antigen Workshop based on its staining pattern of various cell types which was in complete concordance with those exhibited by the reference antibody for the wCD6 cluster at the second workshop, ab36b2. Clone MIL8 and clone ab36b2 immunoprecipitated a monomeric protein of ~150 kDa from an activated T-cell lysate. Further, it was established that clones MIL8 and ab36b2 bind to different epitopes on porcine wCD6 due to their total lack of reciprocal binding to wCD6 expressing cells (Pescovitz et al. 1998).
Product Details
- Target Species
- Pig
- Product Form
- Purified IgG - liquid
- Product Form
- Tissue Culture Supernatant - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Tissue Culture Supernatant containing 0.2M Tris/HCl pH7.4 and 5-10% foetal calf serum
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
0.1% Sodium Azide - Carrier Free
- Yes
- Immunogen
- Porcine Lamina propria leucocytes
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the P3X63 Ag.653 myeloma cell line
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of CD6 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | 1/25 | 1/200 | |
| Immunohistology - Frozen | |||
| Flow Cytometry | Neat | 1/10 | |
| Immunohistology - Frozen |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul
- Flow Cytometry
- Use 50ul of the suggested working dilution to label 106 cells in 100ul.
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Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG2a Negative Control | MCA929 | F | 100 Tests |
|
|
| Mouse IgG2a Negative Control | MCA929 | F | 100 Tests |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse anti Pig CD6 | MCA6047GA | C F IP | 0.1 mg |
|
Product Specific References
References for CD6 antibody
-
de Groot, J. et al. (2000) Immunity in barren and enriched housed pigs differing in baseline cortisol concentration.
Physiol Behav. 71: 217-23. -
Yang, P. et al. (2002) Immune cells in the porcine retina: distribution, characterization and morphological features.
Invest Ophthalmol Vis. 43: 1488-92. -
Pescovitz, M.D. et al. (1998) Analyses of monoclonal antibodies reacting with porcine wCD6: results from the Second International Swine CD Workshop.
Vet Immunol Immunopathol. 60: 285-9. -
Pedersen, L.E. et al. (2016) Expanding specificity of class I restricted CD8+ T cells for viral epitopes following multiple inoculations of swine with a human adenovirus vectored foot-and-mouth disease virus (FMDV) vaccine.
Vet Immunol Immunopathol. 181: 59-67. -
Patch, J.R. et al. (2011) Induction of foot-and-mouth disease virus-specific cytotoxic T cell killing by vaccination.
Clin Vaccine Immunol. 18 (2): 280-8. -
Patch, J.R. et al. (2013) Characterization of cytotoxic T lymphocyte function after foot-and-mouth disease virus infection and vaccination.
Viral Immunol. 26 (4): 239-49. -
Bosschem, I. et al. (2017) Species-specific immunity to Helicobacter suis.
Helicobacter. 22 (3)Jan 26 [Epub ahead of print].
Further Reading
-
Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
Vet Res. 39: 54.
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