CD6 antibody | MIL8
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|Mouse anti Pig wCD6, clone MIL8 reacts with pig wCD6, the porcine homolog of human CD6.
Clone MIL8 was clustered as wCD6 at the Second International Swine CD Antigen Workshop based on its staining pattern of various cell types which was in complete concordance with those exhibited by the reference antibody for the wCD6 cluster at the second workshop, a38b2 (MCA6047). Clone MIL8 and clone a38b2 immunoprecipitated a monomeric protein of ~150 kDa from an activated T-cell lysate. Further, it was established that clones MIL8 and a38b2 bind to different epitopes on porcine wCD6 due to their total lack of reciprocal binding to wCD6 expressing cells (Pescovitz et al. 1998).
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Porcine Lamina propria leucocytes
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the P3X63 Ag.653 myeloma cell line
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Frozen|
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
References for CD6 antibody
de Groot, J. et al. (2000) Immunity in barren and enriched housed pigs differing in baseline cortisol concentration.
Physiol Behav. 71: 217-23.
Yang, P. et al. (2002) Immune cells in the porcine retina: distribution, characterization and morphological features.
Invest Ophthalmol Vis. 43: 1488-92.
Pescovitz, M.D. et al. (1998) Analyses of monoclonal antibodies reacting with porcine wCD6: results from the Second International Swine CD Workshop.
Vet Immunol Immunopathol. 60: 285-9.
Pedersen, L.E. et al. (2016) Expanding specificity of class I restricted CD8+ T cells for viral epitopes following multiple inoculations of swine with a human adenovirus vectored foot-and-mouth disease virus (FMDV) vaccine.
Vet Immunol Immunopathol. 181: 59-67.
Patch, J.R. et al. (2011) Induction of foot-and-mouth disease virus-specific cytotoxic T cell killing by vaccination.
Clin Vaccine Immunol. 18 (2): 280-8.
Patch, J.R. et al. (2013) Characterization of cytotoxic T lymphocyte function after foot-and-mouth disease virus infection and vaccination.
Viral Immunol. 26 (4): 239-49.
Bosschem, I. et al. (2017) Species-specific immunity to Helicobacter suis.
Helicobacter. 22 (3)Jan 26 [Epub ahead of print].
Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
Vet Res. 39: 54.
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