CD6 antibody | MIL8
Mouse anti Pig wCD6
- Product Type
- Monoclonal Antibody
- Clone
- MIL8
- Isotype
- IgG2a
- Specificity
- CD6
Antibody Quality and Performance Guarantee
| Mouse anti Pig wCD6, clone MIL8 reacts with pig wCD6, the porcine homolog of human CD6. Clone MIL8 was clustered as wCD6 at the Second International Swine CD Antigen Workshop based on its staining pattern of various cell types which was in complete concordance with those exhibited by the reference antibody for the wCD6 cluster at the second workshop, a38b2 (MCA6047). Clone MIL8 and clone a38b2 immunoprecipitated a monomeric protein of ~150 kDa from an activated T-cell lysate. Further, it was established that clones MIL8 and a38b2 bind to different epitopes on porcine wCD6 due to their total lack of reciprocal binding to wCD6 expressing cells (Pescovitz et al. 1998). |
- Target Species
- Pig
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Porcine Lamina propria leucocytes
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the P3X63 Ag.653 myeloma cell line
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/25 | 1/200 |
| Immunohistology - Frozen | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG2a Negative Control | MCA929 | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG2a Negative Control | ||||||
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse anti Pig CD6 | MCA6047GA | C F IP | 0.1 mg |
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| List Price | Your Price | ||||||
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| Description | Mouse anti Pig CD6 | ||||||
References for CD6 antibody
-
Pescovitz, M.D. et al. (1998) Analyses of monoclonal antibodies reacting with porcine wCD6: results from the Second International Swine CD Workshop.
Vet Immunol Immunopathol. 60: 285-9. -
de Groot, J. et al. (2000) Immunity in barren and enriched housed pigs differing in baseline cortisol concentration.
Physiol Behav. 71: 217-23. -
Yang, P. et al. (2002) Immune cells in the porcine retina: distribution, characterization and morphological features.
Invest Ophthalmol Vis. 43: 1488-92. -
Patch, J.R. et al. (2011) Induction of foot-and-mouth disease virus-specific cytotoxic T cell killing by vaccination.
Clin Vaccine Immunol. 18 (2): 280-8. -
Patch, J.R. et al. (2013) Characterization of cytotoxic T lymphocyte function after foot-and-mouth disease virus infection and vaccination.
Viral Immunol. 26 (4): 239-49. -
Pedersen, L.E. et al. (2016) Expanding specificity of class I restricted CD8+ T cells for viral epitopes following multiple inoculations of swine with a human adenovirus vectored foot-and-mouth disease virus (FMDV) vaccine.
Vet Immunol Immunopathol. 181: 59-67. -
Bosschem, I. et al. (2017) Species-specific immunity to Helicobacter suis.
Helicobacter. 22 (3): e12375
Further Reading
-
Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
Vet Res. 39: 54.
MCA1221GA
0214
159414
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