CD6 antibody | a38b2

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MCA6047GA
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Mouse anti Pig CD6, clone a38b2, recognizes the porcine homologue of human CD6.

Porcine CD6 is expressed exclusively on T-lymphocytes, clone a38b2 recognizes an epitope on T-lymphocytes which is not present on either monocytes or B-cells (Pauly et al. 1996) . Clone a38b2 immunoprecipitates a monomeric protein of ~150 kDa from an activated T-cell lysate. Clone a38b2 binds to different epitope from clone MIL8 (MCA1221GA) on porcine CD6 due to their total lack of reciprocal binding to CD6 expressing cells (Pescovitz et al. 1998).

Target Species
Pig
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Porcine T-lymphocytes
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/200 1/1000
Immunohistology - Frozen
Immunoprecipitation
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

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References for CD6 antibody

  1. Pauly, T. et al. (1996) Differentiation between MHC-restricted and non-MHC-restricted porcine cytolytic T lymphocytes.
    Immunology. 88 (2): 238-46.
  2. Rau, H. et al. (2006) Immunological properties of recombinant classical swine fever virus NS3 protein in vitro and in vivo.
    Vet Res. 37 (1): 155-68.
  3. Pescovitz, M.D. et al. (1998) Analyses of monoclonal antibodies reacting with porcine wCD6: results from the Second International Swine CD Workshop.
    Vet Immunol Immunopathol. 60 (3-4): 285-9.

UniProt
K7GRE4
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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