CD52 antibody | K263.3D7

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Mouse anti Pig CD52

Product Type
Monoclonal Antibody
Clone
K263.3D7
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA2088GA
Datasheet
F
MSDS
0.1 mg loader

Mouse anti Pig CD52, clone K263.3D7 is a monoclonal antibody recognizing porcine SWC1a, originally assigned at the 1st International Swine Cluster of Differentiation (CD) Workshop.

SWC1a (CD52) is a cell surface molecule which is expressed by most leucocytes including resting T cells, monocytes and granulocytes. SWC1a is not expressed by the majority of B cells, erythrocytes or platelets (Leitner et al. 2012). SWC1a, expressed at very much higher levels on monocytes than on mature macrophages, can be usd with SWC9, expressed exclusively on mature tissue macrophages, to study intermediate stages of monocyte-macrophage differentiation (Sanchez et al. 1999).

Product Details

Target Species
Pig
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Pig peripheral blood mononuclear cells
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
18 months from date of despatch

More Information

Regulatory
For research purposes only

Applications of CD52 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG1 Negative Control MCA928 F 100 Tests loader

Application Based External Images

Flow Cytometry

Product Specific References

References for CD52 antibody

  1. Weesendorp, E. et al. (2013) Comparative analysis of immune responses following experimental infection of pigs with European porcine reproductive and respiratory syndrome virus strains of differing virulence.
    Vet Microbiol. 163 (1-2): 1-12.
  2. Saalmüller A et al. (1994) Analysis of mAb reactive with the porcine SWC1.
    Vet Immunol Immunopathol. 43 (1-3): 255-8.
  3. Sorensen, N.S. et al. (2011) Enhancement of muramyldipeptide (MDP) immunostimulatory activity by controlled multimerization on dendrimers.
    Macromol Biosci. 11 (11): 1484-90.
  4. Tsai, Y.C. et al. (2010) Porcine circovirus type 2 (PCV2) induces cell proliferation, fusion, and chemokine expression in swine monocytic cells in vitro.
    Vet Res. 41 (5): 60.
  5. Tsai, Y-C. et al. (2014) Differences in the Expression of Innate Immune Response-Modulating Genes in Blood Monocytes Between Subclinically Porcine Circovirus Type2 (PCV2)-Infected and PCV2-Free Pigs Prior to and After Lipopolysaccharide Stimulation in vitro.
    Taiwan Vet J. 1450005

Further Reading

  1. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
    Vet Res. 39: 54.
  2. McCullough, K.C. et al. (1999) Intermediate stages in monocyte-macrophage differentiation modulate phenotype and susceptibility to virus infection.
    Immunology 98 (2): 203-12.