Mouse anti Pig CD52, clone K263.3D7
is a monoclonal antibody recognizing porcine SWC1a, originally assigned at the 1st International Swine Cluster of Differentiation (CD) Workshop.
SWC1a (CD52) is a cell surface molecule which is expressed by most leucocytes including resting T cells, monocytes and granulocytes. SWC1a is not expressed by the majority of B cells, erythrocytes or platelets (Leitner et al. 2012
). SWC1a, expressed at very much higher levels on monocytes than on mature macrophages, can be used with SWC9, expressed exclusively on mature tissue macrophages, to study intermediate stages of monocyte-macrophage differentiation (Sanchez et al. 1999
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Pig peripheral blood mononuclear cells
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of CD52 antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
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Secondary Antibodies Available
Negative Isotype Controls Available
Application Based External Images
Product Specific References
References for CD52 antibody
Weesendorp, E. et al. (2013) Comparative analysis of immune responses following experimental infection of pigs with European porcine reproductive and respiratory syndrome virus strains of differing virulence.
Vet Microbiol. 163 (1-2): 1-12.
Saalmüller A et al. (1994) Analysis of mAb reactive with the porcine SWC1.
Vet Immunol Immunopathol. 43 (1-3): 255-8.
Sorensen, N.S. et al. (2011) Enhancement of muramyldipeptide (MDP) immunostimulatory activity by controlled multimerization on dendrimers.
Macromol Biosci. 11 (11): 1484-90.
Tsai, Y.C. et al. (2010) Porcine circovirus type 2 (PCV2) induces cell proliferation, fusion, and chemokine expression in swine monocytic cells in vitro.
Vet Res. 41 (5): 60.
Tsai, Y-C. et al. (2014) Differences in the Expression of Innate Immune Response-Modulating Genes in Blood Monocytes Between Subclinically Porcine Circovirus Type2 (PCV2)-Infected and PCV2-Free Pigs Prior to and After Lipopolysaccharide Stimulation in vitro.
Taiwan Vet J. 1450005
Abu-El-Haija, M. et al. (2011) An activated immune and inflammatory response targets the pancreas of newborn pigs with cystic fibrosis.
Pancreatology. 11 (5): 506-15.
Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
Vet Res. 39: 54.
McCullough, K.C. et al. (1999) Intermediate stages in monocyte-macrophage differentiation modulate phenotype and susceptibility to virus infection.
Immunology 98 (2): 203-12.
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