CD52 antibody | K263.3D7
Mouse anti Pig CD52, clone K263.3D7 (MCA2088GA) used for the evaluation of CD52 (SWC1) expression on monocytes by flow cytometry.
Image caption:
Characterisation of porcine blood monocytes (Mo) and monocyte-derived macrophages (MDM) by flow cytometry following staining with anti-SWC1 (A) and anti-SWC9 (B) mAb. In (A) and (B), the filled-histograms represent the Mo collected on day 0, and the opened histograms represent the MDM after 3 days of incubation with 30% of pooled PCV1-, PCV2-, and PRRSV-free porcine plasma. The data of the positive rates of SWC1 and SWC9 (C) as well as MHC I and MHC II (D) are expressed as percentages and shown as mean ± SD of 6 pigs from three independent experiments in triplicate. *The difference between Mo and MDM is statistically significant (p <0.05). (C) ■: SWC1, □: SWC9; (D) ■: MHC I, □: MHC II.
From: Tsai YC, Jeng CR, Hsiao SH, Chang HW, Liu JJ, Chang CC, Lin CM, Chia MY, Pang VF.
Porcine circovirus type 2 (PCV2) induces cell proliferation, fusion, and chemokine expression in swine monocytic cells in vitro.
Vet Res. 2010 Sep-Oct;41(5):60.
doi: 10.1051/vetres/2010032.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Pig CD52
- Product Type
- Monoclonal Antibody
- Clone
- K263.3D7
- Isotype
- IgG1
- Specificity
- CD52
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| Mouse anti Pig CD52, clone K263.3D7 is a monoclonal antibody recognizing porcine SWC1a, originally assigned at the 1st International Swine Cluster of Differentiation (CD) Workshop. SWC1a (CD52) is a cell surface molecule which is expressed by most leucocytes including resting T cells, monocytes and granulocytes. SWC1a is not expressed by the majority of B cells, erythrocytes or platelets (Leitner et al. 2012). SWC1a, expressed at very much higher levels on monocytes than on mature macrophages, can be used with SWC9, expressed exclusively on mature tissue macrophages, to study intermediate stages of monocyte-macrophage differentiation (Sanchez et al. 1999). |
- Target Species
- Pig
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Pig peripheral blood mononuclear cells
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 1x106 cells in 100μl
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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| Description | Mouse IgG1 Negative Control | ||||||
References for CD52 antibody
-
Weesendorp, E. et al. (2013) Comparative analysis of immune responses following experimental infection of pigs with European porcine reproductive and respiratory syndrome virus strains of differing virulence.
Vet Microbiol. 163 (1-2): 1-12. -
Saalmüller A et al. (1994) Analysis of mAb reactive with the porcine SWC1.
Vet Immunol Immunopathol. 43 (1-3): 255-8. -
Sorensen, N.S. et al. (2011) Enhancement of muramyldipeptide (MDP) immunostimulatory activity by controlled multimerization on dendrimers.
Macromol Biosci. 11 (11): 1484-90. -
Tsai, Y.C. et al. (2010) Porcine circovirus type 2 (PCV2) induces cell proliferation, fusion, and chemokine expression in swine monocytic cells in vitro.
Vet Res. 41 (5): 60. -
Tsai, Y-C. et al. (2014) Differences in the Expression of Innate Immune Response-Modulating Genes in Blood Monocytes Between Subclinically Porcine Circovirus Type2 (PCV2)-Infected and PCV2-Free Pigs Prior to and After Lipopolysaccharide Stimulation in vitro.
Taiwan Vet J. 1450005 -
Abu-El-Haija, M. et al. (2011) An activated immune and inflammatory response targets the pancreas of newborn pigs with cystic fibrosis.
Pancreatology. 11 (5): 506-15. -
Nielsen, O.L. et al. (2021) A porcine model of subcutaneous Staphylococcus aureus. infection: a pilot study.
APMIS. Mar 01 [Epub ahead of print].
Further Reading
-
Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
Vet Res. 39: 54. -
McCullough, K.C. et al. (1999) Intermediate stages in monocyte-macrophage differentiation modulate phenotype and susceptibility to virus infection.
Immunology 98 (2): 203-12.
- Synonyms
- SWC1a
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