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CD45RA antibody | MIL13

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Anti Pig CD45RA Antibody, clone MIL13 thumbnail image 1 Anti Pig CD45RA Antibody, clone MIL13 thumbnail image 2 Anti Pig CD45RA Antibody, clone MIL13 thumbnail image 3 Anti Pig CD45RA Antibody, clone MIL13 thumbnail image 4 Anti Pig CD45RA Antibody, clone MIL13 thumbnail image 5
Anti Pig CD45RA Antibody, clone MIL13 gallery image 1
Figure A. FITC conjugated Mouse anti Porcine CD8b (MCA5954F) and RPE conjugated Mouse IgG1 isotype control (MCA928PE). Figure B. FITC conjugated Mouse anti Porcine CD8b (MCA5954F) and RPE conjugated Mouse anti Porcine CD45RA (MCA1751PE). All experiments performed on red cell lysed porcine blood gated on lymphocytes. Data acquired on the ZE5 Cell Analyzer.
Anti Pig CD45RA Antibody, clone MIL13 gallery image 2
Figure A. FITC conjugated Mouse anti Porcine CD52 (MCA5974F) and RPE conjugated Mouse IgG1 isotype control (MCA928PE). Figure B. FITC conjugated Mouse anti Porcine CD52 (MCA5974F) and RPE conjugated Mouse anti Porcine CD45RA (MCA1751PE). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.
Anti Pig CD45RA Antibody, clone MIL13 gallery image 3
Figure A. RPE conjugated Mouse anti Porcine CD45RA (MCA1751PE) and FITC conjugated Mouse IgG2b isotype control (MCA928F). Figure B. RPE conjugated Mouse anti Porcine CD45RA (MCA1751PE) and FITC conjugated Mouse anti Porcine CD52 (MCA5974F). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.
Anti Pig CD45RA Antibody, clone MIL13 gallery image 4
Figure A. FITC conjugated Mouse anti Porcine CD45RA (MCA1751F) and PE conjugated Mouse IgG1 isotype control (MCA928PE). Figure B. FITC conjugated Mouse anti Porcine CD45RA (MCA1751F) and PE conjugated Mouse anti Porcine CD163 (MCA2311PE). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.
Anti Pig CD45RA Antibody, clone MIL13 gallery image 5
Figure A. PE conjugated Mouse anti Porcine CD163 (MCA2311PE) and FITC conjugated Mouse IgG1 isotype control (MCA928F). Figure B. PE conjugated Mouse anti Porcine CD163 (MCA2311PE) and FITC conjugated Mouse anti Porcine CD45RA (MCA1751F). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.

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Mouse anti Pig CD45RA

Product Type
Monoclonal Antibody
Clone
MIL13
Isotype
IgG1
Specificity
CD45RA

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Product Code Applications Pack Size List Price Your Price Qty
MCA1751GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F P 0.1 mg loader
List Price Your Price
loader

Mouse anti Pig CD45RA, clone MIL13, recognizes an epitope contained in the portion of porcine CD45 encoded by exon A, CD45RA (Lunney et al. 2007).

Mouse anti pig CD45RA, clone MIL13 recognizes both the 210 kDa RA CD45 isoform and the 226 kDa RAC isoform (Zuckermann et al. 2001). Clone MIL13 does not recognize the CD45RC or CD45RO isoforms.

Target Species
Pig
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
Carrier Free
Yes
Immunogen
Cells isolated from porcine mesenteric lymph node
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 1/200
Immunohistology - Frozen
Immunohistology - Paraffin
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 1x106 cells in 100μl

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References for CD45RA antibody

  1. Pakkanen, T.M. et al. (2000) Periadventitial lacZ gene transfer to pig carotid arteries using a biodegradable collagen collar or a wrap of collagen sheet with adenoviruses and plasmid-liposome complexes.
    J Gene Med. 2: 52-60.
  2. Terzic, S. et al. (2002) Immunophenotyping of leukocyte subsets in peripheral blood and palatine tonsils of prefattening pigs.
    Vet Res Commun. 26: 273-83.
  3. Bozić F et al. (2002) Recruitment of intestinal CD45RA+ and CD45RC+ cells induced by a candidate oral vaccine against porcine post-weaning colibacillosis.
    Vet Immunol Immunopathol. 86 (3-4): 137-46.
  4. Schierack, P. et al. (2009) Effects of Bacillus cereus var. toyoi on immune parameters of pregnant sows.
    Vet Immunol Immunopathol. 127: 26-37.
  5. Suzuki, S. et al. (2016) Generation and characterization of RAG2 knockout pigs as animal model for severe combined immunodeficiency.
    Vet Immunol Immunopathol. 178: 37-49.
  6. Li, K.et al. (2019) Generation of porcine monoclonal antibodies based on single cell technologies.
    Vet Immunol Immunopathol. 215: 109913.
  7. López, E. et al. (2019) Identification of very early inflammatory markers in a porcine myocardial infarction model.
    BMC Vet Res. 15 (1): 91.
  8. Forner, R. et al. (2021) Distribution difference of colostrum-derived B and T cells subsets in gilts and sows.
    PLoS One. 16 (5): e0249366.
    9. View The Latest Product References
  9. Zhao, H. et al. (2022) Development of RAG2 -/- IL2Rγ -/Y immune deficient FAH-knockout miniature pig.
    Front Immunol. 13: 950194.
  10. Ogihara, K. et al. (2022) A porcine lymphoma-derived cell line co-expressing IgM, IgG and IgA.
    J Vet Med Sci. Apr 11 [Epub ahead of print].

Further Reading

  1. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
    Vet Res. 39: 54.

MCA1751GA

1114 156541

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