CD8 Beta antibody | PPT23
Mouse anti Pig CD8 Beta Chain:FITC
- Product Type
- Monoclonal Antibody
- Clone
- PPT23
- Isotype
- IgG1
- Specificity
- CD8 Beta
Antibody Quality and Performance Guarantee
| Mouse anti Pig CD8 beta chain, clone PPT23, recognizes the porcine homologue of the human CD8 beta chain cell surface antigen. Characterization of clone PPT23, also known under the clone designation FYP1C5, has demonstrated that on peripheral blood lymphocytes and spleen, this antibody binds to cells that are CD3+, CD4-, CD8hi and as such defines this antibody as a specific marker of porcine α/β T cells, in thymic tissue both CD8lo and CD8hi cells are recognized (Yang & Parkhouse 1997). Mouse anti Pig CD8 beta chain, clone PPT23 recognizes a different epitope on the CD8 beta chain to clone PPT22. |
- Target Species
- Pig
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Immunogen
- Porcine thymus membrane lysate
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Fusion Partners
- Lymph node cells from immunized BALB/c mice were fused with cells of the NS0 myeloma cell line
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) FITC 490 525 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Neat | 1/10 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control:FITC | MCA928F | F | 100 Tests |
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| Description | Mouse IgG1 Negative Control:FITC | ||||||
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse anti Pig CD4 Alpha | MCA1749GA | C * F | 0.1 mg |
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| List Price | Your Price | ||||||
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| Description | Mouse anti Pig CD4 Alpha | ||||||
| Mouse anti Pig CD4 Alpha:RPE | MCA1749PE | F | 100 Tests | Log in | |||
| List Price | Your Price | ||||||
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| Description | Mouse anti Pig CD4 Alpha:RPE | ||||||
| Mouse anti Pig CD3 | MCA5951GA | C F IP WB | 0.1 mg |
|
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| List Price | Your Price | ||||||
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| Description | Mouse anti Pig CD3 | ||||||
| Mouse anti Pig CD3:RPE | MCA5951PE | F | 100 Tests | Log in | |||
| List Price | Your Price | ||||||
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| Description | Mouse anti Pig CD3:RPE | ||||||
Source Reference
-
Yang, H. & Parkhouse, R.M. (1997) Differential expression of CD8 epitopes amongst porcine CD8-positive functional lymphocyte subsets.
Immunology. 92 (1): 45-52.
References for CD8 Beta antibody
-
Sinkora, M. et al. (2007) Two groups of porcine TCRgammadelta+ thymocytes behave and diverge differently.
J Immunol. 178 (2): 711-9. -
Mair, K.H. et al. (2015) Carbopol improves the early cellular immune responses induced by the modified-life vaccine Ingelvac PRRS® MLV.
Vet Microbiol. 176 (3-4): 352-7.
Further Reading
-
Zuckermann, F.A. et al. (1998) Report on the analyses of mAb reactive with porcine CD8 for the second international swine CD workshop.
Vet Immunol Immunopathol. 60 (3-4): 291-303.
MCA5954F
156646
164910
1807
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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