CD25 antibody | K231.3B2




Mouse anti pig CD25, clone K231.3B2 immunoprecipitates a protein of ~65-70 kDa from activated lymphocyte preparations (Bailey et al. 1992).
CD25 is a 270 amino acid single pass type I transmembrane glycoprotein containing 2 Sushi domains. Low expression of CD25 is seen on resting peripheral blood mononuclear cells, rapidly up-regulated following stimulation by concanavalin A and phorbol myristate acetate, indicative of its role as an activation antigen (Bullido et al. 1999).
Product Details
- Target Species
- Pig
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Con A activated porcine peripheral blood lymphocytes.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised mice were fused with cells of the mouse P3-X63-Ag.8.653 myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- O02733
- Entrez Gene
- IL2RA
- GO Terms
- GO:0016021 integral to membrane
- GO:0004872 receptor activity
- Regulatory
- For research purposes only
Applications of CD25 antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | 1/25 | 1/200 | |
Immunohistology - Frozen |
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
Secondary Antibodies Available
Negative Isotype Controls Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
![]() |
Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Mouse anti Pig CD14:FITC | MCA1218F | F | 0.1 mg |
![]() |
|
Mouse anti Pig CD45:Alexa Fluor® 647 | MCA1222A647 | F | 100 Tests/1ml |
![]() |
|
Mouse anti Pig wCD8 Alpha:FITC | MCA1223F | F | 0.1 mg |
![]() |
|
Mouse anti Pig CD4 Alpha:FITC | MCA1749F | F | 0.1 mg |
![]() |
|
Mouse anti Pig wCD8 Alpha:RPE | MCA1223PE | F | 100 Tests |
![]() |
|
Mouse anti Pig CD4 Alpha:RPE | MCA1749PE | F | 100 Tests |
![]() |
Product Specific References
References for CD25 antibody
-
Bailey, M. et al. (1992) A monoclonal antibody recognising an epitope associated with pig interleukin-2 receptors.
J Immunol Methods. 153 (1-2): 85-91. -
Silva-Campa, E. et al. (2010) European genotype of porcine reproductive and respiratory syndrome (PRRSV) infects monocyte-derived dendritic cells but does not induce Treg cells.
Virology. 396 (2): 264-71. -
Kick, A.R. et al. (2011) Evaluation of peripheral lymphocytes after weaning and vaccination for Mycoplasma hyopneumoniae.
Res Vet Sci. 91 (3): e68-72. -
Silva-Campa, E. et al. (2009) Induction of T helper 3 regulatory cells by dendritic cells infected with porcine reproductive and respiratory syndrome virus.
Virology. 387: 373-9. -
Leroith, T. et al. (2011) A modified live PRRSV vaccine and the pathogenic parent strain induce regulatory T cells in pigs naturally infected with Mycoplasma hyopneumoniae.
Vet Immunol Immunopathol.140: 312-6. -
Barker, E. et al. (2006) The larynx as an immunological organ: immunological architecture in the pig as a large animal model.
Clin Exp Immunol. 143: 6-14. -
Kuo, Y.R. et al. (2011) Prolongation of composite tissue allotransplant survival by treatment with bone marrow mesenchymal stem cells is correlated with T-cell regulation in a swine hind-limb model.
Plast Reconstr Surg. 127: 569-79. -
Young, D. et al. (2012) Soy-derived di- and tripeptides alleviate colon and ileum inflammation in pigs with dextran sodium sulfate-induced colitis.
J Nutr. 142: 363-8. -
Chattha, K.S. et al. (2013) Divergent immunomodulating effects of probiotics on T cell responses to oral attenuated human rotavirus vaccine and virulent human rotavirus infection in a neonatal gnotobiotic piglet disease model.
J Immunol. 191: 2446-56. -
Fan, B. et al. (2015) The 15N and 46R Residues of Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus Nucleocapsid Protein Enhance Regulatory T Lymphocytes Proliferation.
PLoS One. 10 (9): e0138772. -
Williams, A.R. et al. (2016) Polymerization-dependent activation of porcine γδ T-cells by proanthocyanidins.
Res Vet Sci. 105: 209-15. -
Suradhat, S. et al. (2016) Transdermal delivery of plasmid encoding truncated nucleocapsid protein enhanced PRRSV-specific immune responses.
Vaccine. 34 (5): 609-15. -
Pan, H. et al. (2016) Lymphodepletive effects of rabbit anti-pig thymocyte globulin in neonatal swines.
Transpl Immunol. 39: 74-83. -
Ferrari, L. et al. (2016) Phenotypic modulation of porcine CD14+ monocytes, natural killer/natural killer T cells and CD8αβ+ T cell subsets by an antibody-derived killer peptide (KP).
Res Vet Sci. 109: 29-39. -
Sirisereewan, C. et al. (2017) Positive immunomodulatory effects of heterologous DNA vaccine- modified live vaccine, prime-boost immunization, against the highly-pathogenic PRRSV infection.
Vet Immunol Immunopathol. 183: 7-15. -
Singleton, H. et al. (2016) Establishing Porcine Monocyte-Derived Macrophage and Dendritic Cell Systems for Studying the Interaction with PRRSV-1.
Front Microbiol. 7: 832. -
An, C.H. et al. (2018) Plant synthetic GP4 and GP5 proteins from porcine reproductive and respiratory syndrome virus elicit immune responses in pigs.
Planta. 247 (4): 973-85. -
Nedumpun, T. et al. (2019) Negative Immunomodulatory Effects of Type 2 Porcine Reproductive and Respiratory Syndrome Virus-Induced Interleukin-1 Receptor Antagonist on Porcine Innate and Adaptive Immune Functions.
Front Immunol. 10: 579.
Further Reading
-
Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
Vet Res. 39: 54.
Fluorescent Spectraviewer
Watch the Tool Tutorial Video ▸
How to Use the Spectraviewer?
Watch the Tool Tutorial Video ▸
- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra