CD25 antibody | K231.3B2
Mouse anti Pig CD25, clone K231.3B2 (MCA1736) used for the evaluation of CD25 expression on lymphocytes in culture by flow cytometry.
Image caption:
Induction of Tregs from lymphocytes co-cultured with PRRSV-infected MoDCs. (A) Representative flow cytometry profile of lymphocytes following 3 days co-culture of PBMCs with PRRSV-infected MoDCs (upper layer), and only culture with PRRSV-infected PBMCs (lower layer). (B) Percentage of Foxp3+ cells in the gated CD4+CD25+ subpopulations of PBMCs co-cultured with PRRSV-infected MoDC. (C) Percentage of Foxp3+ cells in the gated CD4+ CD25+ subpopulations of PRRSV-infected PBMCs alone. (D) Sorted CD4+CD25high cells. (E) Percentage suppression at the indicated Tregs: peripheral lymphocyte ratios. The lymphocytes exposed to Marc-145 lysate-treated MoDCs were used as negative control. The percentage of suppression was calculated as follows: % suppression = 100 × [1 − (% proliferation w/PRRSV/ % proliferation w/mock)] [35]. Data from three independent experiments. All data analysis was done using one-way ANOVA and significant differences are shown (*P<0.05 and **P< 0.01).
From: Fan B, Liu X, Bai J, Li Y, Zhang Q, Jiang P (2015) The 15N and 46R Residues of Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus Nucleocapsid Protein Enhance Regulatory T Lymphocytes Proliferation.
PLoS ONE 10(9): e0138772.
Under a Creative Commons license
Mouse anti Pig CD25, clone K231.3B2 (MCA1736GA) used to identify CD25 expressing cells in porcine lamina propria by immunofluorescence.
Image caption:
Representation of 3 color fluorescence immunohistology of CD4 (red), CD25 (blue), Foxp3 (green), and CD4CD25 (magenta) positive staining in the distal jejunum lamina propria of a 28 day old female and male control (no dietary supplementation) piglets (A). Bar = 10 μm.
From: Christoforidou Z, Mora Ortiz M, Poveda C, Abbas M, Walton G, Bailey M, Lewis MC.
Sexual Dimorphism in Immune Development and in Response to Nutritional Intervention in Neonatal Piglets.
Front Immunol. 2019 Dec 9;10:2705.
doi: 10.3389/fimmu.2019.02705.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
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F IF ResetMouse anti Pig CD25
- Product Type
- Monoclonal Antibody
- Clone
- K231.3B2
- Isotype
- IgG1
- Specificity
- CD25
Antibody Quality and Performance Guarantee
| Mouse anti Pig CD25, clone K231.3B2 recognizes porcine CD25, the alpha chain of the interleukin 2 receptor (IL-2Rα), also known as the low affinity Interleukin 2 receptor. The IL-2 receptor exists in three forms, the high affinity heterodimer, the intermediate affinity β monomer and the low affinity α monomer configurations. Clone K231.3B2 was clustered as CD25 at the First International Workshop to Define Swine Cluster of Differentiation (CD) Antigens (Lunney et al. 1994). Mouse anti pig CD25, clone K231.3B2 immunoprecipitates a protein of ~65-70 kDa from activated lymphocyte preparations (Bailey et al. 1992). CD25 is a 270 amino acid single pass type I transmembrane glycoprotein containing 2 Sushi domains. Low expression of CD25 is seen on resting peripheral blood mononuclear cells, rapidly up-regulated following stimulation by concanavalin A and phorbol myristate acetate, indicative of its role as an activation antigen (Bullido et al. 1999). |
- Target Species
- Pig
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Con A activated porcine peripheral blood lymphocytes.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised mice were fused with cells of the mouse P3-X63-Ag.8.653 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/25 | 1/200 |
| Immunohistology - Frozen | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 1x106 cells in 100μl
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| Mouse anti Pig CD4 Alpha:FITC | MCA1749F | F | 0.1 mg |
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| Description | Mouse anti Pig CD4 Alpha:FITC | ||||||
| Mouse anti Pig CD4 Alpha:RPE | MCA1749PE | F | 100 Tests |
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| Description | Mouse anti Pig CD4 Alpha:RPE | ||||||
| Mouse anti Pig CD14:FITC | MCA1218F | F | 0.1 mg |
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| Description | Mouse anti Pig CD14:FITC | ||||||
References for CD25 antibody
-
Bailey, M. et al. (1992) A monoclonal antibody recognising an epitope associated with pig interleukin-2 receptors.
J Immunol Methods. 153 (1-2): 85-91. -
Silva-Campa, E. et al. (2010) European genotype of porcine reproductive and respiratory syndrome (PRRSV) infects monocyte-derived dendritic cells but does not induce Treg cells.
Virology. 396 (2): 264-71. -
Kick, A.R. et al. (2011) Evaluation of peripheral lymphocytes after weaning and vaccination for Mycoplasma hyopneumoniae.
Res Vet Sci. 91 (3): e68-72. -
Silva-Campa, E. et al. (2009) Induction of T helper 3 regulatory cells by dendritic cells infected with porcine reproductive and respiratory syndrome virus.
Virology. 387: 373-9. -
Leroith, T. et al. (2011) A modified live PRRSV vaccine and the pathogenic parent strain induce regulatory T cells in pigs naturally infected with Mycoplasma hyopneumoniae.
Vet Immunol Immunopathol.140: 312-6. -
Barker, E. et al. (2006) The larynx as an immunological organ: immunological architecture in the pig as a large animal model.
Clin Exp Immunol. 143: 6-14. -
Kuo, Y.R. et al. (2011) Prolongation of composite tissue allotransplant survival by treatment with bone marrow mesenchymal stem cells is correlated with T-cell regulation in a swine hind-limb model.
Plast Reconstr Surg. 127: 569-79. -
Young, D. et al. (2012) Soy-derived di- and tripeptides alleviate colon and ileum inflammation in pigs with dextran sodium sulfate-induced colitis.
J Nutr. 142: 363-8.
View The Latest Product References
-
Chattha, K.S. et al. (2013) Divergent immunomodulating effects of probiotics on T cell responses to oral attenuated human rotavirus vaccine and virulent human rotavirus infection in a neonatal gnotobiotic piglet disease model.
J Immunol. 191: 2446-56. -
Fan, B. et al. (2015) The 15N and 46R Residues of Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus Nucleocapsid Protein Enhance Regulatory T Lymphocytes Proliferation.
PLoS One. 10 (9): e0138772. -
Williams, A.R. et al. (2016) Polymerization-dependent activation of porcine γδ T-cells by proanthocyanidins.
Res Vet Sci. 105: 209-15. -
Suradhat, S. et al. (2016) Transdermal delivery of plasmid encoding truncated nucleocapsid protein enhanced PRRSV-specific immune responses.
Vaccine. 34 (5): 609-15. -
Pan, H. et al. (2016) Lymphodepletive effects of rabbit anti-pig thymocyte globulin in neonatal swines.
Transpl Immunol. 39: 74-83. -
Ferrari, L. et al. (2016) Phenotypic modulation of porcine CD14+ monocytes, natural killer/natural killer T cells and CD8αβ+ T cell subsets by an antibody-derived killer peptide (KP).
Res Vet Sci. 109: 29-39. -
Sirisereewan, C. et al. (2017) Positive immunomodulatory effects of heterologous DNA vaccine- modified live vaccine, prime-boost immunization, against the highly-pathogenic PRRSV infection.
Vet Immunol Immunopathol. 183: 7-15. -
Singleton, H. et al. (2016) Establishing Porcine Monocyte-Derived Macrophage and Dendritic Cell Systems for Studying the Interaction with PRRSV-1.
Front Microbiol. 7: 832. -
An, C.H. et al. (2018) Plant synthetic GP4 and GP5 proteins from porcine reproductive and respiratory syndrome virus elicit immune responses in pigs.
Planta. 247 (4): 973-85. -
Nedumpun, T. et al. (2019) Negative Immunomodulatory Effects of Type 2 Porcine Reproductive and Respiratory Syndrome Virus-Induced Interleukin-1 Receptor Antagonist on Porcine Innate and Adaptive Immune Functions.
Front Immunol. 10: 579. -
Christoforidou, Z. et al. (2019) Sexual Dimorphism in Immune Development and in Response to Nutritional Intervention in Neonatal Piglets.
Front Immunol. 10: 2705. -
Uehlein, S. et al. (2021) Human-like Response of Pig T Cells to Superagonistic Anti-CD28 Monoclonal Antibodies.
J Immunol .ji2100174. -
Nielsen, O.L. et al. (2021) A porcine model of subcutaneous Staphylococcus aureus. infection: a pilot study.
APMIS. Mar 01 [Epub ahead of print]. -
Maciag, S.S. et al. (2022) The influence of source of porcine colostrum in development of early immune ontogeny in the piglet
Ses Sq. Mar 24 [Epub ahead of print].
Further Reading
-
Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
Vet Res. 39: 54.
- Synonyms
- IL-2R Alpha Chain
- UniProt
- O02733
- Entrez Gene
- IL2RA
- GO Terms
- GO:0016021 integral to membrane
- GO:0004872 receptor activity
MCA1736GA
150161
161424
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