CD205 antibody | ZH9F7

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Mouse anti Pig CD205

Product Type
Monoclonal Antibody
Clone
ZH9F7
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA6112GA
Datasheet
F
MSDS
0.1 mg loader

Mouse anti Pig CD205, clone ZH9F7 recognizes the endocytic receptor CD205, also known as DEC205.

CD205 is expressed at high levels by dendritic cell (DC) subsets and can be detected on thymic epithelial cells (Flores-Mendoza et al. 2010).

Mouse anti Pig CD205 antibody, clone ZH9F7, has been used in characterization of the species-conserved features of the cDC1 subset. This subset is characterised by high surface expression of CD205, CD135, CADM1, low levels of CD172a, a lack of CD115, XCR1, and BATF3; and restricted APN gene expression (Auray et al. 2016). CD205 receptor expression was confirmed in cDC1 and cDC2 subsets using flow cytometry on porcine tonsil, submaxillary and mesenteric lymph nodes, and spleen lymphoid tissues (Parra-Sanchez et al. 2018).

Product Details

Target Species
Pig
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Pig CD205
Approx. Protein Concentrations
1.0 mg/ml

Storage Information

Storage
Store at +4°C or at -20°C if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Guarantee
12 months from date of despatch

More Information

Regulatory
For research purposes only

Applications of CD205 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/100
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG1 Negative Control MCA928 F 100 Tests loader

Product Specific References

References for CD205 antibody

  1. Gael, A. et al. (2016) Characterization and Transcriptomic Analysis of Porcine Blood Conventional and Plasmacytoid Dendritic Cells Reveals Striking Species-Specific Differences
    The Journal of Immunology. 197 (12): 4791-4806.
  2. Héctor, P. et al. (2018) Characterization and expression of DEC205 in the cDC1 and cDC2 subsets of porcine dendritic cells from spleen, tonsil, and submaxillary and mesenteric lymph nodes
    Molecular Immunology. 96: 1-7.
  3. Lorena, B. et al. (2019) Evaluation of a Recombinant Mouse X Pig Chimeric Anti-Porcine DEC205 Antibody Fused with Structural and Nonstructural Peptides of PRRS Virus
    Vaccines. 7 (2): 43.

Further Reading

  1. Lilian, F. et al. (2010) Characterization of porcine CD205
    Developmental & Comparative Immunology. 34 (7): 715-721.