CD205 antibody | ZH9F7

Mouse anti Pig CD205

Product Type
Monoclonal Antibody
Clone
ZH9F7
Isotype
IgG1
Specificity
CD205

Product Code Applications Pack Size List Price Your Price Qty
MCA6112GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.1 mg loader
List Price Your Price
loader
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Mouse anti Pig CD205, clone ZH9F7 recognizes the endocytic receptor CD205, also known as DEC205.

CD205 is expressed at high levels by dendritic cell (DC) subsets and can be detected on thymic epithelial cells (Flores-Mendoza et al. 2010).

Mouse anti Pig CD205 antibody, clone ZH9F7, has been used in characterization of the species-conserved features of the cDC1 subset. This subset is characterised by high surface expression of CD205, CD135, CADM1, low levels of CD172a, a lack of CD115, XCR1, and BATF3; and restricted APN gene expression (Auray et al. 2016). CD205 receptor expression was confirmed in cDC1 and cDC2 subsets using flow cytometry on porcine tonsil, submaxillary and mesenteric lymph nodes, and spleen lymphoid tissues (Parra-Sanchez et al. 2018).

Target Species
Pig
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Pig CD205
Approx. Protein Concentrations
1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/100
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul

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References for CD205 antibody

  1. Gael, A. et al. (2016) Characterization and Transcriptomic Analysis of Porcine Blood Conventional and Plasmacytoid Dendritic Cells Reveals Striking Species-Specific Differences
    The Journal of Immunology. 197 (12): 4791-4806.
  2. Héctor, P. et al. (2018) Characterization and expression of DEC205 in the cDC1 and cDC2 subsets of porcine dendritic cells from spleen, tonsil, and submaxillary and mesenteric lymph nodes
    Molecular Immunology. 96: 1-7.
  3. Lorena, B. et al. (2019) Evaluation of a Recombinant Mouse X Pig Chimeric Anti-Porcine DEC205 Antibody Fused with Structural and Nonstructural Peptides of PRRS Virus
    Vaccines. 7 (2): 43.

Further Reading

  1. Lilian, F. et al. (2010) Characterization of porcine CD205
    Developmental & Comparative Immunology. 34 (7): 715-721.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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