MHC Class I H-2Kb antibody | AF6-88.5
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Mouse anti Mouse MHC Class I H-2Kb antibody, clone AF6-88.5 recognizes the H-2Kb determinant present on all nucleated cells of mice expressing this MHC class I haplotype.
The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In mice, this complex is referred to as the histocompatibility 2 (H-2) region. There are 3 major MHC class I proteins encoded by the H-2 which are H-2K, H-2L and H-2D. The H- 2K gene is part of the murine H-2 complex on chromosome 17 and there are a large number of variant alleles of this gene.
Mouse anti Mouse MHC Class I H-2Kb antibody, clone AF6-88.5 does not cross react with other haplotypes.
The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In mice, this complex is referred to as the histocompatibility 2 (H-2) region. There are 3 major MHC class I proteins encoded by the H-2 which are H-2K, H-2L and H-2D. The H- 2K gene is part of the murine H-2 complex on chromosome 17 and there are a large number of variant alleles of this gene.
Mouse anti Mouse MHC Class I H-2Kb antibody, clone AF6-88.5 does not cross react with other haplotypes.
Product Details
- Target Species
- Mouse
- Product Form
- Purified IgG conjugated to fluorescein isothiocyanate (FITC) - liquid.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography
- Preparation
- Purified IgG prepared by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- C57BL mouse splenocytes.
- Approx. Protein Concentrations
- IgG concentration 0.5mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.5mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P01901
- Entrez Gene
- H2-K1
- GO Terms
- GO:0001916 positive regulation of T cell mediated cytotoxicity
- GO:0002485 antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-dependent
- GO:0042742 defense response to bacterium
- GO:0042612 MHC class I protein complex
- GO:0016021 integral to membrane
- GO:0042605 peptide antigen binding
- GO:0006955 immune response
- GO:0009897 external side of plasma membrane
- GO:0042590 antigen processing and presentation of exogenous peptide antigen via MHC class I
- Regulatory
- For research purposes only
Applications of MHC Class I H-2Kb antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | 1/10 | 1/20 | |
| Flow Cytometry | 1/10 | 1/20 | |
| Immunohistology - Frozen | |||
| Immunoprecipitation |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
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Secondary Antibodies Available
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse Seroblock FcR | BUF041A | F | 0.1 mg |
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| Mouse Seroblock FcR | BUF041B | F | 0.5 mg |
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