MHC Class I H-2Kb antibody | AF6-88.5
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Mouse anti Mouse MHC Class I H-2Kb antibody, clone AF6-88.5 recognizes the H-2Kb determinant present on all nucleated cells of mice expressing this MHC class I haplotype.
The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In mice, this complex is referred to as the histocompatibility 2 (H-2) region. There are 3 major MHC class I proteins encoded by the H-2 which are H-2K, H-2L and H-2D. The H- 2K gene is part of the murine H-2 complex on chromosome 17 and there are a large number of variant alleles of this gene.
Mouse anti Mouse MHC Class I H-2Kb antibody, clone AF6-88.5 does not cross react with other haplotypes.
The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In mice, this complex is referred to as the histocompatibility 2 (H-2) region. There are 3 major MHC class I proteins encoded by the H-2 which are H-2K, H-2L and H-2D. The H- 2K gene is part of the murine H-2 complex on chromosome 17 and there are a large number of variant alleles of this gene.
Mouse anti Mouse MHC Class I H-2Kb antibody, clone AF6-88.5 does not cross react with other haplotypes.
Product Details
- Target Species
- Mouse
- Product Form
- Purified IgG conjugated to fluorescein isothiocyanate (FITC) - liquid.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography
- Preparation
- Purified IgG prepared by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- C57BL mouse splenocytes.
- Approx. Protein Concentrations
- IgG concentration 0.5mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.5mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 18 months from date of despatch.
More Information
- UniProt
- P01901 Related reagents
- Entrez Gene
- H2-K1 Related reagents
- GO Terms
- GO:0001916 positive regulation of T cell mediated cytotoxicity
- GO:0002485 antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-dependent
- GO:0042742 defense response to bacterium
- GO:0042612 MHC class I protein complex
- GO:0016021 integral to membrane
- GO:0042605 peptide antigen binding
- GO:0006955 immune response
- GO:0009897 external side of plasma membrane
- GO:0042590 antigen processing and presentation of exogenous peptide antigen via MHC class I
- Regulatory
- For research purposes only
Applications of MHC Class I H-2Kb antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | 1/10 | 1/20 | |
| Flow Cytometry | 1/10 | 1/20 | |
| Immunohistology - Frozen | |||
| Immunoprecipitation |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
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Secondary Antibodies Available
Useful Reagents Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse Seroblock FcR | BUF041A | 0.1 mg | F | ||
| Mouse Seroblock FcR | BUF041B | 0.5 mg | F |
