CD32B antibody | AT130-5
Mouse anti Mouse CD32B
- Product Type
- Monoclonal Antibody
- Clone
- AT130-5
- Isotype
- IgG1
- Specificity
- CD32B
Antibody Quality and Performance Guarantee
| Mouse anti Mouse CD32B, clone AT130-5, recognizes mouse CD32B also known as FcRIIB. CD32B (FcRIIB) is a low affinity receptor for IgG1, IgG2a and IgG2b type antibodies. Unlike other FcRs, the intracellular domain of CD32B contains a inhibitory signalling motif (ITIM) that can antagonize signal transduction mediated through other activatory receptors. FcRIIB is expressed on all myeloid populations and on B-cells. |
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- TRIS buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Mouse CD32-rat CD4 fusion protein.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized mice were fused with cells of the NS-1 myeloma cell line.
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/10 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
References for CD32B antibody
-
White, A. et al. (2011) Interaction with FcγRIIB is critical for the agonistic activity of anti-CD40 monoclonal antibody.
J Immunol. 187: 1754-1763 -
Wiiliams, E.L. et al. (2012) Development and characterisation of monoclonal antibodies specific for the murine inhibitory FcγRIIB (CD32B).
Eur J Immunol. 42: 2109-20 -
Jimenez, R.V. et al. (2018) C-Reactive Protein Impairs Dendritic Cell Development, Maturation, and Function: Implications for Peripheral Tolerance.
Front Immunol. 9: 372. -
Merlo, L.M.F. et al. (2017) Therapeutic antibody targeting of indoleamine-2,3-dioxygenase (IDO2) inhibits autoimmune arthritis.
Clin Immunol. 179: 8-16.
MCA6000
155717
180705
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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