CD28 antibody | E18

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Anti Mouse CD28 Antibody, clone E18 thumbnail image 1

Anti Mouse CD28 Antibody, clone E18 thumbnail image 2

Anti Mouse CD28 Antibody, clone E18 gallery image 1 — Figure A. RPE conjugated rat anti mouse CD45 (MCA1258PE) and Alexa647® conjugated Rat IgG2b isotype control. Figure B. RPE conjugated rat anti mouse CD45 (MCA1258PE) and Alexa647® conjugated rat anti mouse CD28 (**MCA2473A647**). All experiments performed on murine splenocytes in the presence of murine SeroBlock (BUF041A).

Anti Mouse CD28 Antibody, clone E18 gallery image 2 — Figure A. FITC conjugated rat anti mouse CD3 (MCA500F) and RPE conjugated mouse IgG1 isotype control (MCA1209PE). Figure B. FITC conjugated mouse anti mouse CD3 (MCA500F) and RPE conjugated mouse anti mouse CD28 (**MCA2473PE**). All experiments performed on murine splenocytes in the presence of murine SeroBlock (BUF041A).

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Mouse anti Mouse CD28:FITC

Product Type: Monoclonal Antibody
Clone: E18
Isotype: IgG2b
Specificity: CD28

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References (4)
Applications
Useful Reagents
Storage Information
Batch/Lot Specific Datasheets

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Product Code Applications Pack Size List Price Your Price Qty

List Price	Your Price
MCA2473F Datasheet Datasheet SDS SDS	F	0.1 mg		Log in	Get Quote
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Mouse anti Mouse CD28 antibody, clone E18 recognizes murine CD28, a co-stimulatory cell surface molecule expressed by T cell subsets. Clone E18 has been shown to be able to stimulate murine T lymphocytes in combination with anti-mouse CD3.

Target Species: Mouse
Product Form: Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide
1% Bovine Serum Albumin
Immunogen: Alternate immunisation with A20 cells expressing mCD28 and recombinant mCD28Ig.
Approx. Protein Concentrations: IgG concentration 0.1mg/ml
Fusion Partners: Spleen cells from immunised CD28-/-knockout mice were fused with cells of the X63Ag8.653 myeloma cell line.
Max Ex/Em: Fluorophore Excitation Max (nm) Emission Max (nm)

FITC 490 525
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch

Fluorophore	Excitation Max (nm)	Emission Max (nm)
FITC	490	525

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry		Neat	1/5

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul.

Description	Product Code	Applications	Pack Size	Your Price
Mouse Seroblock FcR	BUF041A	F	0.1 mg	Log in	Get Quote
List Price	Your Price
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Description	Mouse Seroblock FcR
Mouse Seroblock FcR	BUF041B	F	0.5 mg	Log in	Get Quote
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Description	Mouse Seroblock FcR

References for CD28 antibody

Dennehy, K.M. et al. (2006) Cutting edge: monovalency of CD28 maintains the antigen dependence of T cell costimulatory responses.
J Immunol. 176 (10): 5725-9.
Beyersdorf, N. et al. (2008) Protection from graft-versus-host disease with a novel B7 binding site-specific mouse anti-mouse CD28 monoclonal antibody.
Blood. 112 (10): 4328-36.
Gogishvili, T. et al. (2012) Interruption of CD28-mediated costimulation during allergen challenge protects mice from allergic airway disease.
J Allergy Clin Immunol. 130 (6): 1394-403.e4.
Pletinckx, K. et al. (2015) Immature dendritic cells convert anergic nonregulatory T cells into Foxp3- IL-10+ regulatory T cells by engaging CD28 and CTLA-4.
Eur J Immunol. 45 (2): 480-91.

RRID: AB_566683
UniProt: P31041
Entrez Gene: Cd28
GO Terms: GO:0002863 positive regulation of inflammatory response to antigenic stimulus; GO:0016021 integral to membrane; GO:0006955 immune response; GO:0008624 induction of apoptosis by extracellular signals; GO:0009897 external side of plasma membrane; GO:0031295 T cell costimulation; GO:0045060 negative thymic T cell selection; GO:0045086 positive regulation of interleukin-2 biosynthetic process; GO:0045840 positive regulation of mitosis; View More GO Terms; GO:0045944 positive regulation of transcription from RNA polymerase II promoter; GO:0046641 positive regulation of alpha-beta T cell proliferation; GO:0048304 positive regulation of isotype switching to IgG isotypes