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CD28 antibody | E18

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Anti Mouse CD28 Antibody, clone E18 thumbnail image 1 Anti Mouse CD28 Antibody, clone E18 thumbnail image 2
Anti Mouse CD28 Antibody, clone E18 gallery image 1
Figure A. RPE conjugated rat anti mouse CD45 (MCA1258PE) and Alexa647® conjugated Rat IgG2b isotype control. Figure B. RPE conjugated rat anti mouse CD45 (MCA1258PE) and Alexa647® conjugated rat anti mouse CD28 (MCA2473A647). All experiments performed on murine splenocytes in the presence of murine SeroBlock (BUF041A).
Anti Mouse CD28 Antibody, clone E18 gallery image 2
Figure A. FITC conjugated rat anti mouse CD3 (MCA500F) and RPE conjugated mouse IgG1 isotype control (MCA1209PE). Figure B. FITC conjugated mouse anti mouse CD3 (MCA500F) and RPE conjugated mouse anti mouse CD28 (MCA2473PE). All experiments performed on murine splenocytes in the presence of murine SeroBlock (BUF041A).

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Mouse anti Mouse CD28:FITC

Product Type
Monoclonal Antibody
Clone
E18
Isotype
IgG2b
Specificity
CD28

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Product Code Applications Pack Size List Price Your Price Qty
MCA2473F
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.1 mg loader
List Price Your Price
loader

Mouse anti Mouse CD28 antibody, clone E18 recognizes murine CD28, a co-stimulatory cell surface molecule expressed by T cell subsets. Clone E18 has been shown to be able to stimulate murine T lymphocytes in combination with anti-mouse CD3.

Target Species
Mouse
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
1% Bovine Serum Albumin
Immunogen
Alternate immunisation with A20 cells expressing mCD28 and recombinant mCD28Ig.
Approx. Protein Concentrations
IgG concentration 0.1mg/ml
Fusion Partners
Spleen cells from immunised CD28-/-knockout mice were fused with cells of the X63Ag8.653 myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/5
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse Seroblock FcR BUF041A F 0.1 mg loader
List Price Your Price
loader
Description Mouse Seroblock FcR
Mouse Seroblock FcR BUF041B F 0.5 mg loader
List Price Your Price
loader
Description Mouse Seroblock FcR

References for CD28 antibody

  1. Dennehy, K.M. et al. (2006) Cutting edge: monovalency of CD28 maintains the antigen dependence of T cell costimulatory responses.
    J Immunol. 176 (10): 5725-9.
  2. Beyersdorf, N. et al. (2008) Protection from graft-versus-host disease with a novel B7 binding site-specific mouse anti-mouse CD28 monoclonal antibody.
    Blood. 112 (10): 4328-36.
  3. Gogishvili, T. et al. (2012) Interruption of CD28-mediated costimulation during allergen challenge protects mice from allergic airway disease.
    J Allergy Clin Immunol. 130 (6): 1394-403.e4.
  4. Pletinckx, K. et al. (2015) Immature dendritic cells convert anergic nonregulatory T cells into Foxp3- IL-10+ regulatory T cells by engaging CD28 and CTLA-4.
    Eur J Immunol. 45 (2): 480-91.

RRID
AB_566683
UniProt
P31041
Entrez Gene
Cd28
GO Terms
GO:0002863 positive regulation of inflammatory response to antigenic stimulus
GO:0016021 integral to membrane
GO:0006955 immune response
GO:0008624 induction of apoptosis by extracellular signals
GO:0009897 external side of plasma membrane
GO:0031295 T cell costimulation
GO:0045060 negative thymic T cell selection
GO:0045086 positive regulation of interleukin-2 biosynthetic process
GO:0045840 positive regulation of mitosis
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
GO:0046641 positive regulation of alpha-beta T cell proliferation
GO:0048304 positive regulation of isotype switching to IgG isotypes

MCA2473F

148519

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