IgA antibody | IgA5-3B
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Mouse anti Monkey IgA antibody, clone IgA5-3B recognizes monkey IgA and does not cross-react with IgG or IgM.
Clone IgA5-3B has been shown to detect secreted IgA in cat saliva and rhesus monkey jejunal washes.
Clone IgA5-3B has been shown to detect secreted IgA in cat saliva and rhesus monkey jejunal washes.
Product Details
- Target Species
- Monkey
- Species Cross-Reactivity
-
Target Species Cross Reactivity Cat Rhesus Monkey Dog Human Marmoset Spotted Hyena - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Biotin - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
0.05% Sodium Azide (NaN3) - Immunogen
- Purified IgA from serum.
- Approx. Protein Concentrations
- IgG concentration 0.5mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of IgA antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | |||
| ELISA | |||
| Western Blotting |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- ELISA
- This product may be used in a direct ELISA or as a detection antibody in a sandwich ELISA together with product code MCA1891 as a capture reagent.
- Western Blotting
- MCA2553 detects a band of approximately 55kDa under reducing conditions.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Product Specific References
References for IgA antibody
-
Beatty, J.A. et al. (1998) Feline immunodeficiency virus (FIV)-associated lymphoma: a potential role for immune dysfunction in tumourigenesis.
Vet Immunol Immunopathol. 65 (2-4): 309-22. -
Grant, C.K. (1995) Purification and characterization of feline IgM and IgA isotypes and three subclasses of IgG. In: Feline immunology and immunodeficiency, Eds. Willett, B.J. and Jarrett, O.
Oxford University Press pp. 95-107. -
Grant, C.K. (1995) Immunoglobulin changes associated with feline immunodeficiency virus infections. In: Feline immunology and immunodeficiency Eds. Willett, B.J. and Jarrett, O.
Oxford University Press pp. 170-189. -
Flies, A.S. et al. (2012) Development of a hyena immunology toolbox.
Vet Immunol Immunopathol. 145 (1-2): 110-9. -
Holbrook, B.C. et al. (2015) Nonhuman primate infants have an impaired respiratory but not systemic IgG antibody response following influenza virus infection.
Virology. 476: 124-33.
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