VEGF antibody | 4B2-8
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Mouse anti Human VEGF:Preservative Free
- Product Type
- Monoclonal Antibody
- Clone
- 4B2-8
- Isotype
- IgG1
- Specificity
- VEGF
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| Mouse anti Human Vascular endothelial growth factor antibody, clone 4B2-8 recognizes human Vascular endothelial growth factor A , also known as VEGF-A or Vascular permeability factor. VEGF-A is a 206 amino acid ~45 kDa disulphide linked homodimeric heparin-binding cytokine and member of the PDGF/VEGF growth factor family, which exists as several splice variants of the VEGF gene, and is a potent stimulator of angiogenesis of both normal and cancerous cells, and acts as a regulator of vasculogenesis. The functional forms of VEGF are classified according to the number of amino acid residues within a constitutive polypeptide chain e.g VEGF165, and all VEGF family members act through binding to the tyrosine kinase receptors VEGFR-1 (Flt-1) and VEGFR-2 (KDR/CD309), whilst the closely-related VEGF-C and VEGF-D, can also bind to VEGFR-3 (Flt-4). |
- Target Species
- Human
- Product Form
- Purified IgG - lyophilized
- Reconstitution
- Reconstitute with 0.5 ml distilled waterCare should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Carrier Free
- Yes
- Immunogen
- Highly pure recombinant human VEGF165 (PHP091).
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml after reconstitution
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
2.0 | 4.0ug/ml |
| Functional Assays | |
0.1 | 0.2ug/ml |
| Western Blotting | |
0.25 | 0.50ug/ml |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Synonyms
- VPF
- RRID
- AB_10897188
- UniProt
- P15692
- Entrez Gene
- VEGFA
View more products with VEGF specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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