VEGF antibody | 4B2-8

Mouse anti Human VEGF:Preservative Free

Product Type
Monoclonal Antibody
Clone
4B2-8
Isotype
IgG1
Specificity
VEGF

Product Code Applications Pack Size List Price Your Price Qty
MCA5750Z
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E FN WB 0.5 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Mouse anti Human Vascular endothelial growth factor antibody, clone 4B2-8 recognizes human Vascular endothelial growth factor A , also known as VEGF-A or Vascular permeability factor. VEGF-A is a 206 amino acid ~45 kDa disulphide linked homodimeric heparin-binding cytokine and member of the PDGF/VEGF growth factor family, which exists as several splice variants of the VEGF gene, and is a potent stimulator of angiogenesis of both normal and cancerous cells, and acts as a regulator of vasculogenesis.

The functional forms of VEGF are classified according to the number of amino acid residues within a constitutive polypeptide chain e.g VEGF165, and all VEGF family members act through binding to the tyrosine kinase receptors VEGFR-1 (Flt-1) and VEGFR-2 (KDR/CD309), whilst the closely-related VEGF-C and VEGF-D, can also bind to VEGFR-3 (Flt-4).

Target Species
Human
Product Form
Purified IgG - lyophilized
Reconstitution
Reconstitute with 0.5 ml distilled water

Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Carrier Free
Yes
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml after reconstitution
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.

This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 2.0 4.0ug/ml
Functional Assays 0.1 0.2ug/ml
Western Blotting 0.25 0.50ug/ml
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
ELISA
MCA5750Z may be used in a sandwich ELISA with AHP831B as the detection antibody and PHP091 as the standard.
Western Blotting
MCA5750Z detects a band of approximately 38.2kDa using recombinant human VEGF165 PHP091 under non-reducing conditions.

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Synonyms
VPF
RRID
AB_10897188
UniProt
P15692
Entrez Gene
VEGFA
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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