VEGF

//www.bio-rad-antibodies.com/human-vegf-recombinant-protein-php091.html?RenderPageSpecific=1
VEGF gallery image 1

Purchased this product? Why not be the first to submit a review and upload an image?

Enlarge
  • VEGF thumbnail image 1
  • Recombinant Human VEGF
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Recombinant Protein
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    PHP091E, FN, WBdatasheet pdfdatasheet pdf10 µg
    PHP091
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • VEGF is a homodimeric protein produced by various vascularized tissues. Activities include stimulation of endothelial cell growth, angiogenesis and capillary permeability.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified recombinant protein expressed in E. coli - lyophilised
    • Reconstitution
      Reconstitute with 0.1 ml distilled water
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
    • Preparation
    • Preservative Stabilisers
      None present
    • Purity
      >98% by SDS PAGE and HPLC analysis
    • Approx. Protein Concentrations
      0.1 mg/ml
    • Protein Molecular Weight
      38kD (2 x 165 Amino acid sequence)
    • Reagents In The Kit
    • Preparing The Antibody
    • Endotoxin Level
      <0.1 ng/ug
    • Test Principle
    • Buffer Solution
    • Storage
      Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of reconstitution.
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.20.4ng/well
      Functional Assays1100ng/ml
      Western Blotting1.53.0ng/lane

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the protein for use in their own system using appropriate positive/negative controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
      PHP091 may be used in ELISA applications with either AHP831 or AHP831B.
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      PHP091 may be used under reducing or non-reducing western blotting conditions with either AHP831 or AHP831B.
    • Instructions For Use

    Additional VEGF Formats

    Formats Applications Sizes available
    VEGF : Rec. Protein E, FN, WB 10 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Goat anti Human VEGF:BiotinAHP831B50 µgE, WB
          AHP831B
          Goat anti Human VEGFAHP8310.1 mgE, FN, P *, WB
          AHP831

          Recommended Positive Controls

            Histology Controls

              References

              Write your review

              You may also be interested in...