VEGF is a homodimeric protein produced by various vascularized tissues. Activities include stimulation of endothelial cell growth, angiogenesis and capillary permeability.
Purified recombinant protein expressed in E. coli - lyophilised
Reconstitute with 0.1 ml distilled water Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
>98% by SDS PAGE and HPLC analysis
Approx. Protein Concentrations
Protein Molecular Weight
38kD (2 x 165 Amino acid sequence)
Reagents In The Kit
Preparing The Antibody
Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the protein for use in their own system using appropriate positive/negative controls.
PHP091 may be used in ELISA applications with either AHP831 or AHP831B.
Histology Positive Control Tissue
PHP091 may be used under reducing or non-reducing western blotting conditions with either AHP831 or AHP831B.