Thyroid Stimulating Hormone Receptor antibody | 2C11
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Mouse anti Human Thyroid Stimulating Hormone Receptor
- Product Type
- Monoclonal Antibody
- Clone
- 2C11
- Isotype
- IgG1
- Specificity
- Thyroid Stimulating Hormone Receptor
Antibody Quality and Performance Guarantee
| Mouse anti Human thyroid stimulating hormone receptor antibody, clone 2C11 recognizes the human thyroid stimulating hormone receptor (TSHR) otherwise known as the thyrotropin receptor. TSHR is an important molecule in controlling the growth and function of the normal thyroid. Mouse anti Human thyroid stimulating receptor antibody recognises both native and denatured TSH receptor (binding to an epitope at the carboxy terminus between amino acids 354 and 359). It does inhibit binding of TSH. No cross reactivity has been observed with related LH and FSH receptors. Mouse anti Human thyroid stimulating hormone receptor antibody, clone 2C11 recognizes the mutant TSH receptor known as I167N as well as the wild type molecule (Costagliola et al. 1998) |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Recombinant Human TSH receptor.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the NS1/Ag4.1 mouse myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
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| Flow Cytometry | |
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| Immunohistology - Frozen | |||
| Immunoprecipitation | |
5ug/ml | 10ug/ml |
| Western Blotting | |
5ug/ml | 10ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control | ||||||
Source Reference
-
Johnstone, A.P. et al. (1994) Monoclonal antibodies that recognize the native human thyrotropin receptor.
Mol Cell Endocrinol. 105 (2): R1-9.
References for Thyroid Stimulating Hormone Receptor antibody
-
Costagliola, S. et al. (1998) Genetic immunization against the human thyrotropin receptor causes thyroiditis and allows production of monoclonal antibodies recognizing the native receptor.
J Immunol. 160 (3): 1458-65. -
Shepherd PS et al. (1999) Identification of an important thyrotrophin binding site on the human thyrotrophin receptor using monoclonal antibodies.
Mol Cell Endocrinol. 149 (1-2): 197-206. -
Akamizu, T. et al. (1999) Characterization of recombinant monoclonal antithyrotropin receptor antibodies (TSHRAbs) derived from lymphocytes of patients with Graves' disease: epitope and binding study of two stimulatory TSHRAbs.
Endocrinology. 140 (4): 1594-601. -
Chen, C.R. et al. (2001) A full biological response to autoantibodies in Graves' disease requires a disulfide-bonded loop in the thyrotropin receptor N terminus homologous to a laminin epidermal growth factor-like domain.
J Biol Chem. 276 (18): 14767-72. -
Chazenbalk, G.D. et al. (2002) Thyroid-stimulating autoantibodies in Graves disease preferentially recognize the free A subunit, not the thyrotropin holoreceptor.
J Clin Invest. 110: 209-17. -
Chen, C.R. et al. (2003) Targeted restoration of cleavage in a noncleaving thyrotropin receptor demonstrates that cleavage is insufficient to enhance ligand-independent activity.
Endocrinology. 144: 1324-30. -
Chazenbalk, G.D. et al. (2004) Does thyrotropin cleave its cognate receptor?
Endocrinology. 145 (1): 4-10. -
Li, Y.S. et al. (2004) Transgenic mice producing major histocompatibility complex class II molecules on thyroid cells do not develop apparent autoimmune thyroid diseases.
Endocrinology. 145: 2524-30.
View The Latest Product References
-
Neumann S et al. (2005) Structural determinants for g protein activation and selectivity in the second intracellular loop of the thyrotropin receptor.
Endocrinology. 146 (1): 477-85. -
Claus, M. et al. (2005) A hydrophobic cluster in the center of the third extracellular loop is important for thyrotropin receptor signaling.
Endocrinology. 146 (12): 5197-203. -
Ellerhorst, J.A. et al. (2006) Human melanoma cells express functional receptors for thyroid-stimulating hormone.
Endocr Relat Cancer. 13: 1269-77. -
Frenzel, R. et al. (2006) The human thyrotropin receptor is predominantly internalized by beta-arrestin 2.
Endocrinology. 147: 3114-22. -
Akeno, N. et al. (2011) IFN-{alpha} Mediates the Development of Autoimmunity both by Direct Tissue Toxicity and through Immune Cell Recruitment Mechanisms.
J Immunol. 186: 4693-706. -
Neumann, S. et al. (2011) A new small-molecule antagonist inhibits Graves' disease antibody activation of the TSH receptor.
J Clin Endocrinol Metab. 96: 548-54. -
Narumi S et al. (2011) Nonclassic TSH resistance: TSHR mutation carriers with discrepantly high thyroidal iodine uptake.
J Clin Endocrinol Metab. 96 (8): E1340-5. -
Allen, M.D. et al. (2011) Occupancy of both sites on the thyrotropin (TSH) receptor dimer is necessary for phosphoinositide signaling.
FASEB J. 25: 3687-94. -
Haas, A.K. et al. (2011) Mutations that silence constitutive signaling activity in the allosteric ligand-binding site of the thyrotropin receptor.
Cell Mol Life Sci. 68: 159-167. -
Read, M.L. et al. (2011) Proto-oncogene PBF/PTTG1IP regulates thyroid cell growth and represses radioiodide treatment.
Cancer Res. 71 (19): 6153-64. -
Krause, K. et al. (2012) Comparative proteomic analysis to dissect differences in signal transduction in activating TSH receptor mutations in the thyroid.
Int J Biochem Cell Biol. 44 (2): 290-301.
- RRID
- AB_2208261
- UniProt
- P16473
- Entrez Gene
- TSHR
- GO Terms
- GO:0005515 protein binding
- GO:0004996 thyroid-stimulating hormone receptor activity
- GO:0005887 integral to plasma membrane
- GO:0007187 G-protein signaling, coupled to cyclic nucleotide second messenger
- GO:0007267 cell-cell signaling
- GO:0008284 positive regulation of cell proliferation
MCA1281
148874
155644
157433
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