Thyroid Stimulating Hormone Receptor antibody | 2C11

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Mouse anti Human Thyroid Stimulating Hormone Receptor

Product Type
Monoclonal Antibody
Clone
2C11
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1281 E F IP P WB 0.2 mg
Mouse anti Human thyroid stimulating hormone receptor antibody, clone 2C11 recognizes the human thyroid stimulating hormone receptor (TSHR) otherwise known as the thyrotropin receptor. TSHR is an important molecule in controlling the growth and function of the normal thyroid.

Mouse anti Human thyroid stimulating receptor antibody recognises both native and denatured TSH receptor (binding to an epitope at the carboxy terminus between amino acids 354 and 359). It does inhibit binding of TSH. No cross reactivity has been observed with related LH and FSH receptors.

Mouse anti Human thyroid stimulating hormone receptor antibody, clone 2C11 recognizes the mutant TSH receptor known as I167N as well as the wild type molecule (Costagliola et al. 1998)

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Recombinant Human TSH receptor.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the NS1/Ag4.1 mouse myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P16473 Related reagents
Entrez Gene
TSHR Related reagents
GO Terms
GO:0005515 protein binding
GO:0004996 thyroid-stimulating hormone receptor activity
GO:0005887 integral to plasma membrane
GO:0007187 G-protein signaling, coupled to cyclic nucleotide second messenger
GO:0007267 cell-cell signaling
GO:0008284 positive regulation of cell proliferation
Regulatory
For research purposes only

Applications of Thyroid Stimulating Hormone Receptor antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation 5ug/ml 10ug/ml
Western Blotting 5ug/ml 10ug/ml
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG1 Negative Control MCA928 100 Tests F

Application Based External Images

Flow Cytometry

Immunohistology - Paraffin

Western Blotting

Product Specific References

References for Thyroid Stimulating Hormone Receptor antibody

  1. Shepherd PS et al. (1999) Identification of an important thyrotrophin binding site on the human thyrotrophin receptor using monoclonal antibodies.
    Mol Cell Endocrinol. 149 (1-2): 197-206.
  2. Costagliola, S. et al. (1998) Genetic immunization against the human thyrotropin receptor causes thyroiditis and allows production of monoclonal antibodies recognizing the native receptor.
    J Immunol. 160 (3): 1458-65.
  3. Haas, A.K. et al. (2011) Mutations that silence constitutive signaling activity in the allosteric ligand-binding site of the thyrotropin receptor.
    Cell Mol Life Sci. 68: 159-167.
  4. Frenzel, R. et al. (2006) The human thyrotropin receptor is predominantly internalized by beta-arrestin 2.
    Endocrinology. 147: 3114-22.
  5. Akeno, N. et al. (2011) IFN-{alpha} Mediates the Development of Autoimmunity both by Direct Tissue Toxicity and through Immune Cell Recruitment Mechanisms.
    J Immunol. 186: 4693-706.
  6. Akamizu, T. et al. (1999) Characterization of recombinant monoclonal antithyrotropin receptor antibodies (TSHRAbs) derived from lymphocytes of patients with Graves' disease: epitope and binding study of two stimulatory TSHRAbs.
    Endocrinology. 140: 1594-601.
  7. Chazenbalk, G.D. et al. (2004) Does thyrotropin cleave its cognate receptor?
    Endocrinology. 145: 4-10.
  8. Chen, C.R. et al. (2003) Targeted restoration of cleavage in a noncleaving thyrotropin receptor demonstrates that cleavage is insufficient to enhance ligand-independent activity.
    Endocrinology. 144: 1324-30.
  9. Ellerhorst, J.A. et al. (2006) Human melanoma cells express functional receptors for thyroid-stimulating hormone.
    Endocr Relat Cancer. 13: 1269-77.
  10. Li, Y.S. et al. (2004) Transgenic mice producing major histocompatibility complex class II molecules on thyroid cells do not develop apparent autoimmune thyroid diseases.
    Endocrinology. 145: 2524-30.
  11. Neumann, S. et al. (2011) A new small-molecule antagonist inhibits Graves' disease antibody activation of the TSH receptor.
    J Clin Endocrinol Metab. 96: 548-54.
  12. Narumi S et al. (2011) Nonclassic TSH resistance: TSHR mutation carriers with discrepantly high thyroidal iodine uptake.
    J Clin Endocrinol Metab. 96 (8): E1340-5.
  13. Allen, M.D. et al. (2011) Occupancy of both sites on the thyrotropin (TSH) receptor dimer is necessary for phosphoinositide signaling.
    FASEB J. 25: 3687-94.