Talin antibody | TA205
Mouse anti Human Talin antibody, clone TA205 recognizes human talin, a ~225 kDa cytoskeletal protein. Mouse anti Human Talin antibody, clone TA205 may be used to stain adhesion plaques in fixed and permeabilized fibroblasts. Mouse anti Human Talin antibody, clone recognizes an epitope between residues 139 and 433.
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity Rabbit Chicken
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein G
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Human platelet talin.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0 mouse myeloma cell line.
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- Shelf Life
- 18 months from date of despatch.
- Q9Y490 Related reagents
- Q9Y4G6 Related reagents
- Entrez Gene
- TLN1 Related reagents
- TLN2 Related reagents
- GO Terms
- GO:0002576 platelet degranulation
- GO:0006936 muscle contraction
- GO:0003779 actin binding
- GO:0007155 cell adhesion
- GO:0005576 extracellular region
- GO:0005829 cytosol
- GO:0005158 insulin receptor binding
- GO:0005200 structural constituent of cytoskeleton
- GO:0005813 centrosome
- GO:0005911 cell-cell junction
- GO:0005925 focal adhesion
- GO:0006928 cellular component movement
- GO:0007016 cytoskeletal anchoring at plasma membrane
- GO:0007043 cell-cell junction assembly
- GO:0007411 axon guidance
- GO:0015629 actin cytoskeleton
- GO:0017166 vinculin binding
- GO:0030168 platelet activation
- GO:0030274 LIM domain binding
- GO:0032587 ruffle membrane
- GO:0043231 intracellular membrane-bounded organelle
- GO:0001726 ruffle
- GO:0005737 cytoplasm
- GO:0045202 synapse
- For research purposes only
Applications of Talin antibody
|Application Name||Verified||Min Dilution||Max Dilution|
|Flow Cytometry 1||1/25||1/100|
|Immunohistology - Frozen|
- 1 Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells
- Histology Positive Control Tissue
- Most tissues
- Western Blotting
- Clone TA205 has also been reported to work in Western blotting.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
|Description||Product Code||Pack Size||Applications||List Price||Quantity|
|Mouse IgG1 Negative Control||MCA928||100 Tests||F|
Product Specific References
References for Talin antibody
Porter, R.M. et al. (1993) Monoclonal antibodies to cytoskeletal proteins: an immunohistochemical investigation of human colon cancer.
J Pathol. 170 (4): 435-40.
Bolton, S.J. et al. (1997) Monoclonal antibodies recognizing the N- and C-terminal regions of talin disrupt actin stress fibers when microinjected into human fibroblasts.
Cell Motil Cytoskeleton. 36 (4): 363-76.
Zheng, P.P. et al. (2007) Hela l-CaD is implicated in the migration of endothelial cells/endothelial progenitor cells in human neoplasms.
Cell Adh Migr. 1: 84-91.
Vainionpää, N. et al. (2006) Laminin-10 and Lutheran blood group glycoproteins in adhesion of human endothelial cells.
Am J Physiol Cell Physiol. 290: C764-75.
(2003) Production of Fibronectin and Tenascin Isoforms and Their Role in the Adhesion of Human Immortalized Corneal Epithelial Cells
Invest Ophthalmol Vis Sci. 44: 3317-25.
Calderwood, D.A. et al. (1999) The Talin head domain binds to integrin beta subunit cytoplasmic tails and regulates integrin activation.
J Biol Chem. 274: 28071-4.
Cui, L. et al. (2009) c-Abl kinase is required for beta 2 integrin-mediated neutrophil adhesion.
J Immunol. 182: 3233-42.
Koch, S. et al. (2013) Cathepsin D deficiency induces cytoskeletal changes and affects cell migration pathways in the brain.
Neurobiol Dis. 50: 107-19.
Hasenson, S. et al. (2005) The immortalized human corneal epithelial cells adhere to laminin-10 by using Lutheran glycoproteins and integrin alpha3beta1.
Exp Eye Res. 81 (4): 415-21.
Braga, V.M. et al. (1995) Calcium-induced changes in distribution and solubility of cadherins, integrins and their associated cytoplasmic proteins in human keratinocytes.
Cell Adhes Commun. 3 (3): 201-15.
Valmu, L. et al. (1999) The cytoskeletal association of CD11/CD18 leukocyte integrins in phorbol ester-activated cells correlates with CD18 phosphorylation.
Eur J Immunol. 29 (7): 2107-18.
Zhang C et al. (2013) c-Abl Kinase Is a Regulator of αvβ3 Integrin Mediated Melanoma A375 Cell Migration.
PLoS One. 8 (6): e66108.