Talin antibody | TA205

Mouse anti Human Talin

Product Type
Monoclonal Antibody
Clone
TA205
Isotype
IgG1
Specificity
Talin

Product Code Applications Pack Size List Price Your Price Qty
MCA725G
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F * IF IP WB 0.2 mg loader
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loader
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Mouse anti Human Talin antibody, clone TA205 recognizes human talin, a ~225 kDa cytoskeletal protein. Mouse anti Human Talin antibody, clone TA205 may be used to stain adhesion plaques in fixed and permeabilized fibroblasts. Mouse anti Human Talin antibody, clone recognizes an epitope between residues 139 and 433 and has been sucessfully used in western blotting applications (Bolton et al. 1997).

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rabbit
Chicken
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Carrier Free
Yes
Immunogen
Human platelet talin.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0 mouse myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1 1/25 1/100
Immunofluorescence
Immunohistology - Frozen
Immunoprecipitation
Western Blotting
  1. 1 Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells.

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Source Reference

  1. Bolton, S.J. et al. (1997) Monoclonal antibodies recognizing the N- and C-terminal regions of talin disrupt actin stress fibers when microinjected into human fibroblasts.
    Cell Motil Cytoskeleton. 36 (4): 363-76.

References for Talin antibody

  1. Porter, R.M. et al. (1993) Monoclonal antibodies to cytoskeletal proteins: an immunohistochemical investigation of human colon cancer.
    J Pathol. 170 (4): 435-40.
  2. Calderwood, D.A. et al. (1999) The Talin head domain binds to integrin beta subunit cytoplasmic tails and regulates integrin activation.
    J Biol Chem. 274: 28071-4.
  3. Zheng, P.P. et al. (2007) Hela l-CaD is implicated in the migration of endothelial cells/endothelial progenitor cells in human neoplasms.
    Cell Adh Migr. 1: 84-91.
  4. Vainionpää, N. et al. (2006) Laminin-10 and Lutheran blood group glycoproteins in adhesion of human endothelial cells.
    Am J Physiol Cell Physiol. 290: C764-75.
  5. Filenius, S. (2003) Production of Fibronectin and Tenascin Isoforms and Their Role in the Adhesion of Human Immortalized Corneal Epithelial Cells
    Invest Ophthalmol Vis Sci. 44: 3317-25.
  6. Hasenson, S. et al. (2005) The immortalized human corneal epithelial cells adhere to laminin-10 by using Lutheran glycoproteins and integrin alpha3beta1.
    Exp Eye Res. 81 (4): 415-21.
  7. Cui, L. et al. (2009) c-Abl kinase is required for beta 2 integrin-mediated neutrophil adhesion.
    J Immunol. 182: 3233-42.
  8. Koch, S. et al. (2013) Cathepsin D deficiency induces cytoskeletal changes and affects cell migration pathways in the brain.
    Neurobiol Dis. 50: 107-19.
  9. View The Latest Product References
  10. Valmu, L. et al. (1999) The cytoskeletal association of CD11/CD18 leukocyte integrins in phorbol ester-activated cells correlates with CD18 phosphorylation.
    Eur J Immunol. 29 (7): 2107-18.
  11. Braga, V.M. et al. (1995) Calcium-induced changes in distribution and solubility of cadherins, integrins and their associated cytoplasmic proteins in human keratinocytes.
    Cell Adhes Commun. 3 (3): 201-15.
  12. Zhang C et al. (2013) c-Abl Kinase Is a Regulator of αvβ3 Integrin Mediated Melanoma A375 Cell Migration.
    PLoS One. 8 (6): e66108.

Immunofluorescence

Western Blotting

RRID
AB_2233001
UniProt
Q9Y490
Q9Y4G6
Entrez Gene
TLN1
TLN2
GO Terms
GO:0002576 platelet degranulation
GO:0006936 muscle contraction
GO:0003779 actin binding
GO:0007155 cell adhesion
GO:0005576 extracellular region
GO:0005829 cytosol
GO:0005158 insulin receptor binding
GO:0005200 structural constituent of cytoskeleton
GO:0005813 centrosome
GO:0005911 cell-cell junction
GO:0005925 focal adhesion
GO:0006928 cellular component movement
GO:0007016 cytoskeletal anchoring at plasma membrane
GO:0007043 cell-cell junction assembly
GO:0007411 axon guidance
GO:0015629 actin cytoskeleton
GO:0017166 vinculin binding
GO:0030168 platelet activation
GO:0030274 LIM domain binding
GO:0032587 ruffle membrane
GO:0043231 intracellular membrane-bounded organelle
GO:0001726 ruffle
GO:0005737 cytoplasm
GO:0045202 synapse
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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