Talin antibody | TA205
Mouse anti Human talin antibody, clone TA205 (MCA725G) used to isolate and recognize talin from A375 human melanoma cell line lysates by immunoprecipitation and western blotting respectively
Image caption:
c-Abl kinase directly associates with talin head domain and regulates β3 integrin ligation concomitantly after PDGF stimulation.
(A) Overnight serum-starved A375 cells were either untreated or treated with 20 ng/ml PDGF for the indicated times. Thereafter, the cells were lysed, and the lysates were immunoblotted with indicated antibodies. In inhibition experiments, cells were preincubated with STI571 for 10 min, and the inhibitor remained in the medium throughout the assay period. (B) Serum-starved A375 cells were stimulated as above. The lysates were immunoprecipitated with anti-talin antibody and immunoblotted with indicated antibodies. (C) Serum-starved A375 cells were treated as above. The lysates were incubated with GST or GST fusion proteins, and the bound proteins were separated by SDS-PAGE and immunoblotted with indicated antibodies. (D) GST or GST fusion proteins were incubated with Ni2+ Sepharose coated with His-talin2-head. Bound proteins were separated by SDS-PAGE and immunoblotted with anti-GST antibody. The immunoblotting bands were quantified by densitometry, and data were normalized with respect to the controls. Data are representative of three independent experiments.
From: Zhang C, Yang C, Wang R, Jiao Y, Ampah KK, Wang X, et al. (2013)
c-Abl Kinase Is a Regulator of αvβ3 Integrin Mediated Melanoma A375 Cell Migration.
PLoS ONE 8(6): e66108.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human Talin
- Product Type
- Monoclonal Antibody
- Clone
- TA205
- Isotype
- IgG1
- Specificity
- Talin
Antibody Quality and Performance Guarantee
| Mouse anti Human Talin antibody, clone TA205 recognizes human talin, a ~225 kDa cytoskeletal protein. Mouse anti Human Talin antibody, clone TA205 may be used to stain adhesion plaques in fixed and permeabilized fibroblasts. Mouse anti Human Talin antibody, clone recognizes an epitope between residues 139 and 433 and has been sucessfully used in western blotting applications (Bolton et al. 1997). |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rabbit Chicken - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3).
- Carrier Free
- Yes
- Immunogen
- Human platelet talin.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0 mouse myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry 1 |  |
1/25 | 1/100 |
| Immunofluorescence | |
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| Immunohistology - Frozen | |
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| Immunoprecipitation | |
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| Western Blotting | |
- 1 Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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Log in | ||
| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control | ||||||
Source Reference
-
Bolton, S.J. et al. (1997) Monoclonal antibodies recognizing the N- and C-terminal regions of talin disrupt actin stress fibers when microinjected into human fibroblasts.
Cell Motil Cytoskeleton. 36 (4): 363-76.
References for Talin antibody
-
Porter, R.M. et al. (1993) Monoclonal antibodies to cytoskeletal proteins: an immunohistochemical investigation of human colon cancer.
J Pathol. 170 (4): 435-40. -
Braga, V.M. et al. (1995) Calcium-induced changes in distribution and solubility of cadherins, integrins and their associated cytoplasmic proteins in human keratinocytes.
Cell Adhes Commun. 3 (3): 201-15. -
Calderwood, D.A. et al. (1999) The Talin head domain binds to integrin beta subunit cytoplasmic tails and regulates integrin activation.
J Biol Chem. 274: 28071-4. -
Valmu, L. et al. (1999) The cytoskeletal association of CD11/CD18 leukocyte integrins in phorbol ester-activated cells correlates with CD18 phosphorylation.
Eur J Immunol. 29 (7): 2107-18. -
Filenius, S.
(2003) Production of Fibronectin and Tenascin Isoforms and Their Role in the Adhesion of Human Immortalized Corneal Epithelial Cells
Invest Ophthalmol Vis Sci. 44: 3317-25. -
Hasenson, S. et al. (2005) The immortalized human corneal epithelial cells adhere to laminin-10 by using Lutheran glycoproteins and integrin alpha3beta1.
Exp Eye Res. 81 (4): 415-21. -
Vainionpää, N. et al. (2006) Laminin-10 and Lutheran blood group glycoproteins in adhesion of human endothelial cells.
Am J Physiol Cell Physiol. 290: C764-75. -
Zheng, P.P. et al. (2007) Hela l-CaD is implicated in the migration of endothelial cells/endothelial progenitor cells in human neoplasms.
Cell Adh Migr. 1: 84-91.
View The Latest Product References
-
Cui, L. et al. (2009) c-Abl kinase is required for beta 2 integrin-mediated neutrophil adhesion.
J Immunol. 182: 3233-42. -
Koch, S. et al. (2013) Cathepsin D deficiency induces cytoskeletal changes and affects cell migration pathways in the brain.
Neurobiol Dis. 50: 107-19. -
Zhang C et al. (2013) c-Abl Kinase Is a Regulator of αvβ3 Integrin Mediated Melanoma A375 Cell Migration.
PLoS One. 8 (6): e66108.
- RRID
- AB_2233001
- UniProt
- Q9Y490
- Q9Y4G6
- Entrez Gene
- TLN1
- TLN2
- GO Terms
- GO:0002576 platelet degranulation
- GO:0006936 muscle contraction
- GO:0003779 actin binding
- GO:0007155 cell adhesion
- GO:0005576 extracellular region
- GO:0005829 cytosol
- GO:0005158 insulin receptor binding
- GO:0005200 structural constituent of cytoskeleton
- GO:0005813 centrosome
- View More GO Terms
- GO:0005911 cell-cell junction
- GO:0005925 focal adhesion
- GO:0006928 cellular component movement
- GO:0007016 cytoskeletal anchoring at plasma membrane
- GO:0007043 cell-cell junction assembly
- GO:0007411 axon guidance
- GO:0015629 actin cytoskeleton
- GO:0017166 vinculin binding
- GO:0030168 platelet activation
- GO:0030274 LIM domain binding
- GO:0032587 ruffle membrane
- GO:0043231 intracellular membrane-bounded organelle
- GO:0001726 ruffle
- GO:0005737 cytoplasm
- GO:0045202 synapse
MCA725G
150624
151575
161369
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