Mouse anti Human Talin antibody, clone TA205 recognizes human talin, a ~225 kDa cytoskeletal protein. Mouse anti Human Talin antibody, clone TA205 may be used to stain adhesion plaques in fixed and permeabilized fibroblasts. Mouse anti Human Talin antibody, clone recognizes an epitope between residues 139 and 433.
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein G
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Human platelet talin.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0 mouse myeloma cell line.
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
insulin receptor binding
structural constituent of cytoskeleton
cellular component movement
cytoskeletal anchoring at plasma membrane
cell-cell junction assembly
LIM domain binding
intracellular membrane-bounded organelle
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of Talin antibody
|Flow Cytometry 1
|Immunohistology - Frozen
- 1 Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm� (Product Code BUF09) for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells
- Histology Positive Control Tissue
- Most tissues
- Western Blotting
- Clone TA205 has also been reported to work in Western blotting.
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Secondary Antibodies Available
Negative Isotype Controls Available
Application Based External Images
Product Specific References
References for Talin antibody
Porter, R.M. et al. (1993) Monoclonal antibodies to cytoskeletal proteins: an immunohistochemical investigation of human colon cancer.
J Pathol. 170 (4): 435-40.
Bolton, S.J. et al. (1997) Monoclonal antibodies recognizing the N- and C-terminal regions of talin disrupt actin stress fibers when microinjected into human fibroblasts.
Cell Motil Cytoskeleton. 36 (4): 363-76.
Zheng, P.P. et al. (2007) Hela l-CaD is implicated in the migration of endothelial cells/endothelial progenitor cells in human neoplasms.
Cell Adh Migr. 1: 84-91.
Vainionpää, N. et al. (2006) Laminin-10 and Lutheran blood group glycoproteins in adhesion of human endothelial cells.
Am J Physiol Cell Physiol. 290: C764-75.
Filenius, S. (2003) Production of Fibronectin and Tenascin Isoforms and Their Role in the Adhesion of Human Immortalized Corneal Epithelial Cells
Invest Ophthalmol Vis Sci. 44: 3317-25.
Calderwood, D.A. et al. (1999) The Talin head domain binds to integrin beta subunit cytoplasmic tails and regulates integrin activation.
J Biol Chem. 274: 28071-4.
Cui, L. et al. (2009) c-Abl kinase is required for beta 2 integrin-mediated neutrophil adhesion.
J Immunol. 182: 3233-42.
Koch, S. et al. (2013) Cathepsin D deficiency induces cytoskeletal changes and affects cell migration pathways in the brain.
Neurobiol Dis. 50: 107-19.
Hasenson, S. et al. (2005) The immortalized human corneal epithelial cells adhere to laminin-10 by using Lutheran glycoproteins and integrin alpha3beta1.
Exp Eye Res. 81 (4): 415-21.
Braga, V.M. et al. (1995) Calcium-induced changes in distribution and solubility of cadherins, integrins and their associated cytoplasmic proteins in human keratinocytes.
Cell Adhes Commun. 3 (3): 201-15.
Valmu, L. et al. (1999) The cytoskeletal association of CD11/CD18 leukocyte integrins in phorbol ester-activated cells correlates with CD18 phosphorylation.
Eur J Immunol. 29 (7): 2107-18.
Zhang C et al. (2013) c-Abl Kinase Is a Regulator of αvβ3 Integrin Mediated Melanoma A375 Cell Migration.
PLoS One. 8 (6): e66108.