Talin antibody | TA205

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Mouse anti Human Talin

Product Type
Monoclonal Antibody
Clone
TA205
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA725G
Datasheet
C F* IF IP WB
MSDS
0.2 mg loader

Mouse anti Human Talin antibody, clone TA205 recognizes human talin, a ~225 kDa cytoskeletal protein. Mouse anti Human Talin antibody, clone TA205 may be used to stain adhesion plaques in fixed and permeabilized fibroblasts. Mouse anti Human Talin antibody, clone recognizes an epitope between residues 139 and 433.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rabbit
Chicken
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Human platelet talin.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0 mouse myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
18 months from date of despatch.

More Information

UniProt
Q9Y490 Related reagents
Q9Y4G6 Related reagents
Entrez Gene
TLN1 Related reagents
TLN2 Related reagents
GO Terms
GO:0002576 platelet degranulation
GO:0006936 muscle contraction
GO:0003779 actin binding
GO:0007155 cell adhesion
GO:0005576 extracellular region
GO:0005829 cytosol
GO:0005158 insulin receptor binding
GO:0005200 structural constituent of cytoskeleton
GO:0005813 centrosome
GO:0005911 cell-cell junction
GO:0005925 focal adhesion
GO:0006928 cellular component movement
GO:0007016 cytoskeletal anchoring at plasma membrane
GO:0007043 cell-cell junction assembly
GO:0007411 axon guidance
GO:0015629 actin cytoskeleton
GO:0017166 vinculin binding
GO:0030168 platelet activation
GO:0030274 LIM domain binding
GO:0032587 ruffle membrane
GO:0043231 intracellular membrane-bounded organelle
GO:0001726 ruffle
GO:0005737 cytoplasm
GO:0045202 synapse
Regulatory
For research purposes only

Applications of Talin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1 1/25 1/100
Immunofluorescence
Immunohistology - Frozen
Immunoprecipitation
Western Blotting
  1. 1 Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm (Product Code BUF09) for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells
Histology Positive Control Tissue
Most tissues
Western Blotting
Clone TA205 has also been reported to work in Western blotting.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG1 Negative Control MCA928 F 100 Tests loader

Application Based External Images

Immunofluorescence

Western Blotting

Product Specific References

References for Talin antibody

  1. Porter, R.M. et al. (1993) Monoclonal antibodies to cytoskeletal proteins: an immunohistochemical investigation of human colon cancer.
    J Pathol. 170 (4): 435-40.
  2. Bolton, S.J. et al. (1997) Monoclonal antibodies recognizing the N- and C-terminal regions of talin disrupt actin stress fibers when microinjected into human fibroblasts.
    Cell Motil Cytoskeleton. 36 (4): 363-76.
  3. Zheng, P.P. et al. (2007) Hela l-CaD is implicated in the migration of endothelial cells/endothelial progenitor cells in human neoplasms.
    Cell Adh Migr. 1: 84-91.
  4. Vainionpää, N. et al. (2006) Laminin-10 and Lutheran blood group glycoproteins in adhesion of human endothelial cells.
    Am J Physiol Cell Physiol. 290: C764-75.
  5. Filenius, S. (2003) Production of Fibronectin and Tenascin Isoforms and Their Role in the Adhesion of Human Immortalized Corneal Epithelial Cells
    Invest Ophthalmol Vis Sci. 44: 3317-25.
  6. Calderwood, D.A. et al. (1999) The Talin head domain binds to integrin beta subunit cytoplasmic tails and regulates integrin activation.
    J Biol Chem. 274: 28071-4.
  7. Cui, L. et al. (2009) c-Abl kinase is required for beta 2 integrin-mediated neutrophil adhesion.
    J Immunol. 182: 3233-42.
  8. Koch, S. et al. (2013) Cathepsin D deficiency induces cytoskeletal changes and affects cell migration pathways in the brain.
    Neurobiol Dis. 50: 107-19.
  9. Hasenson, S. et al. (2005) The immortalized human corneal epithelial cells adhere to laminin-10 by using Lutheran glycoproteins and integrin alpha3beta1.
    Exp Eye Res. 81 (4): 415-21.
  10. Braga, V.M. et al. (1995) Calcium-induced changes in distribution and solubility of cadherins, integrins and their associated cytoplasmic proteins in human keratinocytes.
    Cell Adhes Commun. 3 (3): 201-15.
  11. Valmu, L. et al. (1999) The cytoskeletal association of CD11/CD18 leukocyte integrins in phorbol ester-activated cells correlates with CD18 phosphorylation.
    Eur J Immunol. 29 (7): 2107-18.
  12. Zhang C et al. (2013) c-Abl Kinase Is a Regulator of αvβ3 Integrin Mediated Melanoma A375 Cell Migration.
    PLoS One. 8 (6): e66108.
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